TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SelQ_toxoplasma_gondii_gladyshev # QUERY (66 letters) Database: /users/rg/didac/GENOMES/F.cylindrus/genome.fa 271 sequences; 80,540,407 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value scaffold_46 32 0.23 scaffold_6 32 0.23 scaffold_71 30 0.52 scaffold_1 28 2.0 >scaffold_46 Length = 481344 Score = 31.6 bits (70), Expect = 0.23 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = +3 Query: 25 QPEHRRTWQFRGTIPQNPHLAPRFRPNVNDRYQIRRGR 62 +P RR W T NPHL FRP ++Y++R+ R Sbjct: 191328 RPLIRRFWPVTSTDDTNPHLV--FRPREKEKYKLRKKR 191435 Score = 28.9 bits (63), Expect = 1.5 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +3 Query: 25 QPEHRRTWQFRGTIPQNPH 43 Q E RRTW F G IP+N H Sbjct: 458925 Q*ECRRTWNFHGRIPRNYH 458981 >scaffold_6 Length = 2967614 Score = 31.6 bits (70), Expect = 0.23 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = +2 Query: 25 QPEHRRTWQFRGTIPQNPHLAPRFRPNVNDRYQIRRGR 62 +P RR W T NPHL FRP ++Y++R+ R Sbjct: 1971800 RPLIRRFWPVTSTDDTNPHLV--FRPREKEKYKLRKKR 1971907 >scaffold_71 Length = 263701 Score = 30.4 bits (67), Expect = 0.52 Identities = 11/28 (39%), Positives = 19/28 (67%) Frame = -2 Query: 39 PQNPHLAPRFRPNVNDRYQIRRGRGGUC 66 P+ PH+A + +++ RY++RR R G C Sbjct: 212043 PKKPHIATTWDLSLDHRYRLRRSRHGTC 211960 >scaffold_1 Length = 5926375 Score = 28.5 bits (62), Expect = 2.0 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -1 Query: 39 PQNPHLAPRFRPNVNDRYQIRRGRGGUC 66 P+ PH+A +++ RY++RR R G C Sbjct: 3974383 PKKPHIATT*DLSLDHRYRLRRSRHGTC 3974300 Database: /users/rg/didac/GENOMES/F.cylindrus/genome.fa Posted date: Sep 29, 2011 10:40 PM Number of letters in database: 80,540,407 Number of sequences in database: 271 Lambda K H 0.326 0.143 0.484 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,493,673 Number of Sequences: 271 Number of extensions: 44931 Number of successful extensions: 294 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 271 Number of HSP's gapped (non-prelim): 39 length of query: 66 length of database: 26,846,802 effective HSP length: 41 effective length of query: 25 effective length of database: 26,835,691 effective search space: 670892275 effective search space used: 670892275 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 56 (26.2 bits)