TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|166240590|ref|XP_643546.2| thyroxine 5'-deiodinase [Dictyostelium discoideum AX4] (257 letters) Database: S.arctica/genome.fa 15,618 sequences; 121,588,341 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.1013 of Sphaeroforma arctica JP610 32 2.0 supercont1.882 of Sphaeroforma arctica JP610 31 3.5 supercont1.1135 of Sphaeroforma arctica JP610 30 5.9 supercont1.127 of Sphaeroforma arctica JP610 30 5.9 >supercont1.1013 of Sphaeroforma arctica JP610 Length = 26287 Score = 31.6 bits (70), Expect = 2.0 Identities = 30/104 (28%), Positives = 45/104 (43%), Gaps = 7/104 (6%) Frame = +1 Query: 6 NIFEEEKPIKKAPIITEECYRNGYHPSKWELAQVLLNGIWKPYKFKSYMQYKSR------ 59 ++F KPI I ECY N H S WE ++ + +M+ +R Sbjct: 25156 SVFRSCKPIASCRCIWRECYPNCPHQSGWEANVTE*ISAYENH*M--HMRLNTRLWHIVV 25329 Query: 60 -ERLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDR 102 ERL L ++ IL DD + QE L N N+ K +++R Sbjct: 25330 IERLRLTSNAY---*ILYDDGSTQERFADMLANGNDEFKFIRNR 25452 >supercont1.882 of Sphaeroforma arctica JP610 Length = 34137 Score = 30.8 bits (68), Expect = 3.5 Identities = 27/105 (25%), Positives = 43/105 (40%) Frame = -3 Query: 96 EKSVQDRPMVLICGSFTUPPFRDKMCMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGD 155 ++ V+DR ++ C F P F+DKM +EI+P+ W+ G Sbjct: 25234 DRGVEDRDVLTACIKFVVPKFKDKM-----------------NTPAEEINPSASWHPTGT 25106 Query: 156 EISLCYRQPKTMEDRREIIKDLKEYAPFCTIPFLIDKMDNNFNKV 200 S QP + RE+ + + P TIP + NF+ V Sbjct: 25105 PASW---QPTS---TRELSEQMHAAGPIRTIP----QRSTNFHHV 25001 >supercont1.1135 of Sphaeroforma arctica JP610 Length = 21729 Score = 30.0 bits (66), Expect = 5.9 Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 5/37 (13%) Frame = -2 Query: 78 DQTNQEVSLYDLF-----NSNNNEKSVQDRPMVLICG 109 +QTN++ S+Y ++ N NNN K +R + L+CG Sbjct: 13442 EQTNKK*SVYSIYLWPSKNVNNNMKCENERKIPLVCG 13332 >supercont1.127 of Sphaeroforma arctica JP610 Length = 142785 Score = 30.0 bits (66), Expect = 5.9 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = -2 Query: 79 QTNQEVSLYDLFNSNNNEKSVQDRPMVLICGSFTUPPFRD 118 +TN+ +S N N NE V +R +V+IC F + D Sbjct: 90392 KTNERISTRSASNMNLNEIGVLERSIVIICAQFVN*QYPD 90273 Database: S.arctica/genome.fa Posted date: Nov 21, 2011 7:47 PM Number of letters in database: 121,588,341 Number of sequences in database: 15,618 Lambda K H 0.321 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 41,436,616 Number of Sequences: 15618 Number of extensions: 684418 Number of successful extensions: 2346 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1173 Number of HSP's successfully gapped in prelim test: 114 Number of HSP's that attempted gapping in prelim test: 940 Number of HSP's gapped (non-prelim): 1697 length of query: 257 length of database: 40,529,447 effective HSP length: 107 effective length of query: 150 effective length of database: 38,858,321 effective search space: 5828748150 effective search space used: 5828748150 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 64 (29.3 bits)