TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|166240590|ref|XP_643546.2| thyroxine 5'-deiodinase
[Dictyostelium discoideum AX4]
         (257 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig5077  12231 40311                                                44   0.001
Contig7521  5445 6810                                                  39   0.030
Contig48363  488 488                                                   33   1.3  
Contig997  24542 24642                                                 31   6.4  

>Contig5077  12231 40311
          Length = 40311

 Score = 43.5 bits (101), Expect = 0.001
 Identities = 18/51 (35%), Positives = 29/51 (56%)
 Frame = +2

Query: 101  DRPMVLICGSFTUPPFRDKMCMFQDIFVDFKEWVDIYIVYLKEIHPADEWY 151
            D+P+V+I GS++ PP R  +     +  +F E +    +Y+ E H  DEWY
Sbjct: 4532 DKPLVIIGGSYS*PPMRFVVPQLHALQKEFSEKMSFVCIYIAEAHATDEWY 4684


>Contig7521  5445 6810
          Length = 6810

 Score = 38.5 bits (88), Expect = 0.030
 Identities = 32/120 (26%), Positives = 46/120 (38%), Gaps = 40/120 (33%)
 Frame = -2

Query: 130 FKEWVDIYIVYLKEIHPADEWYIG---------------------GDE--------ISLC 160
           F   VD   VY+ E H +DEW +G                     G E        +++C
Sbjct: 413 FASEVDFLGVYISEAHASDEWPLGIKVFKNKNKNNKIVNKK*GREGREREGEGVRFLTVC 234

Query: 161 -----------YRQPKTMEDRREIIKDLKEYAPFCTIPFLIDKMDNNFNKVYDAVPERLY 209
                      Y QPK ME R +I         F  +P L+D M+N F+  + + PER +
Sbjct: 233 FLTFFVFF*YVYEQPKEMETRLKIANGFVNDFKF-NLPMLVDTMENEFDSHFASWPERYF 57


>Contig48363  488 488
          Length = 488

 Score = 33.1 bits (74), Expect = 1.3
 Identities = 20/52 (38%), Positives = 28/52 (53%)
 Frame = -3

Query: 116 FRDKMCMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGDEISLCYRQPKTM 167
           F +   +F+ ++  F+E V    VY+ E H  DEW IG D   L   QPKT+
Sbjct: 174 FTNSKVLFR-MYEAFQEKVKFLTVYILEAHTQDEWPIGDD--VLLATQPKTV 28


>Contig997  24542 24642
          Length = 24642

 Score = 30.8 bits (68), Expect = 6.4
 Identities = 15/53 (28%), Positives = 28/53 (52%)
 Frame = -3

Query: 119  KMCMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGDEISLCYRQPKTMEDRR 171
            +M +   + + F+++   Y +YL  I  +  W++GG      YRQP+ +  RR
Sbjct: 8557 EMLIHLHVPISFRKYF*KYFIYLLNIV*SANWFLGG------YRQPEVVHGRR 8417


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.321    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 109,680,227
Number of Sequences: 297657
Number of extensions: 1558126
Number of successful extensions: 5148
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 5013
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5147
length of query: 257
length of database: 106,339,350
effective HSP length: 111
effective length of query: 146
effective length of database: 73,299,423
effective search space: 10701715758
effective search space used: 10701715758
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)