TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|166240590|ref|XP_643546.2| thyroxine 5'-deiodinase [Dictyostelium discoideum AX4] (257 letters) Database: P.capsici/genome.fa 917 sequences; 64,023,748 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PHYCAscaffold_14 29 5.7 PHYCAscaffold_139 28 9.6 PHYCAscaffold_126 28 9.6 PHYCAscaffold_33 28 9.6 >PHYCAscaffold_14 Length = 1100894 Score = 29.3 bits (64), Expect = 5.7 Identities = 11/34 (32%), Positives = 21/34 (61%) Frame = -2 Query: 154 GDEISLCYRQPKTMEDRREIIKDLKEYAPFCTIP 187 G ++SLCYR+ +T D+ +++K+ C +P Sbjct: 24226 GGKVSLCYREMRTAMDKTASSRNIKQELG*CNMP 24125 >PHYCAscaffold_139 Length = 29350 Score = 28.5 bits (62), Expect = 9.6 Identities = 16/52 (30%), Positives = 31/52 (59%), Gaps = 1/52 (1%) Frame = +2 Query: 56 YKSR-ERLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDRPMVL 106 Y+S+ E + ++DT+ PDA + ++ + + LF SN+N S++ P+ L Sbjct: 25781 YQSKTEPVEPESDTKMPDASDVSEERESKKWIKQLFKSNSNWISMKIHPIKL 25936 >PHYCAscaffold_126 Length = 72628 Score = 28.5 bits (62), Expect = 9.6 Identities = 16/52 (30%), Positives = 31/52 (59%), Gaps = 1/52 (1%) Frame = +1 Query: 56 YKSR-ERLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDRPMVL 106 Y+S+ E + ++DT+ PDA + ++ + + LF SN+N S++ P+ L Sbjct: 40306 YQSKTEPVEPESDTKMPDASDVSEERESKKWIKQLFKSNSNWISMKIHPIKL 40461 >PHYCAscaffold_33 Length = 640966 Score = 28.5 bits (62), Expect = 9.6 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 3/49 (6%) Frame = +1 Query: 8 FEEEK--PIKKAP-IITEECYRNGYHPSKWELAQVLLNGIWKPYKFKSY 53 +EE K P+ AP +I + G+HP W L + LL+ IWK + Y Sbjct: 138688 YEEMKASPVLMAPSLIHRDPLNTGHHPRTWALIRRLLD-IWKRLQVSYY 138831 Database: P.capsici/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 64,023,748 Number of sequences in database: 917 Lambda K H 0.321 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 19,247,639 Number of Sequences: 917 Number of extensions: 265217 Number of successful extensions: 999 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 39 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 852 Number of HSP's gapped (non-prelim): 346 length of query: 257 length of database: 21,341,249 effective HSP length: 103 effective length of query: 154 effective length of database: 21,246,798 effective search space: 3272006892 effective search space used: 3272006892 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.5 bits)