TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|166240590|ref|XP_643546.2| thyroxine 5'-deiodinase
[Dictyostelium discoideum AX4]
         (257 letters)

Database: D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|256541943|gb|CM000151.3| Dictyostelium discoideum AX4 chromos...   518   e-147
gi|93569080|gb|CM000153.2| Dictyostelium discoideum AX4 chromoso...    31   0.82 
gi|93569058|gb|CM000155.2| Dictyostelium discoideum AX4 chromoso...    30   1.8  
gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromoso...    29   4.1  

>gi|256541943|gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2,
               whole genome shotgun sequence
          Length = 8484197

 Score =  518 bits (1334), Expect = e-147
 Identities = 248/257 (96%), Positives = 248/257 (96%)
 Frame = +3

Query: 1       MNYLFNIFEEEKPIKKAPIITEECYRNGYHPSKWELAQVLLNGIWKPYKFKSYMQYKSRE 60
               MNYLFNIFEEEKPIKKAPIITEECYRNGYHPSKWELAQVLLNGIWKPYKFKSYMQYKSRE
Sbjct: 5884722 MNYLFNIFEEEKPIKKAPIITEECYRNGYHPSKWELAQVLLNGIWKPYKFKSYMQYKSRE 5884901

Query: 61      RLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDRPMVLICGSFTUPPFRDKM 120
               RLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDRPMVLICGSFT PPFRDKM
Sbjct: 5884902 RLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQDRPMVLICGSFT*PPFRDKM 5885081

Query: 121     CMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGDEISLCYRQPKTMEDRREIIKDLKEY 180
               CMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGDEISLCYRQPKTMEDRREIIKDLKEY
Sbjct: 5885082 CMFQDIFVDFKEWVDIYIVYLKEIHPADEWYIGGDEISLCYRQPKTMEDRREIIKDLKEY 5885261

Query: 181     APFCTIPFLIDKMDNNFNKVYDAVPERLYVLEDKKFKYVXXXXXXXXIPEELREFLTKRY 240
               APFCTIPFLIDKMDNNFNKVYDAVPERLYVLEDKKFKYV        IPEELREFLTKRY
Sbjct: 5885262 APFCTIPFLIDKMDNNFNKVYDAVPERLYVLEDKKFKYVGGPGPFGFIPEELREFLTKRY 5885441

Query: 241     KPHLLDLKNNLSIPPSQ 257
               KPHLLDLKNNLSIPPSQ
Sbjct: 5885442 KPHLLDLKNNLSIPPSQ 5885492



 Score = 29.6 bits (65), Expect = 2.4
 Identities = 15/39 (38%), Positives = 25/39 (64%)
 Frame = -1

Query: 77      DDQTNQEVSLYDLFNSNNNEKSVQDRPMVLICGSFTUPP 115
               D+++N+ ++L DL   ++ EKS+ +RP   I  SFT  P
Sbjct: 5484164 DEKSNKIINLKDLLQQSSIEKSIYNRP---IQSSFTVTP 5484057


>gi|93569080|gb|CM000153.2| Dictyostelium discoideum AX4 chromosome 4,
              whole genome shotgun sequence
          Length = 5450249

 Score = 31.2 bits (69), Expect = 0.82
 Identities = 14/30 (46%), Positives = 19/30 (63%)
 Frame = +2

Query: 174    IKDLKEYAPFCTIPFLIDKMDNNFNKVYDA 203
              I+D+K      T PF+ D +DNNF K YD+
Sbjct: 553442 IRDIKLTLFILTTPFITDILDNNFYKHYDS 553531


>gi|93569058|gb|CM000155.2| Dictyostelium discoideum AX4 chromosome 6, whole
               genome shotgun sequence
          Length = 3602379

 Score = 30.0 bits (66), Expect = 1.8
 Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 5/60 (8%)
 Frame = +1

Query: 42      NGIWKPYKFKSY----MQYKSRERLNLQADTQCPDAILLDDQTNQ-EVSLYDLFNSNNNE 96
               N +WKP   K +    + +K R  ++ ++  Q  ++I  +D TN  +    +LFN+NNNE
Sbjct: 3350560 NTLWKPLYIKKWNVITLPHKKRISID-KSQNQIKNSISFNDFTNYYQDDKNELFNNNNNE 3350736


>gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromosome 3, whole
               genome shotgun sequence
          Length = 6357299

 Score = 28.9 bits (63), Expect = 4.1
 Identities = 20/60 (33%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
 Frame = -3

Query: 48      YKFKSYMQYKSRERLNLQADTQCPDAILLDDQTNQEVSLYDLF-NSNNNEKSVQDRPMVL 106
               ++ K Y +Y S +RLN  +  Q    I   D  NQ +SL++ + N NN EK  Q   +++
Sbjct: 5315796 FEIKQY-EYNSIKRLNKGSKKQINRLIKTLDLKNQFLSLFENYINDNNIEKLTQANKILI 5315620


  Database: D.discoideum_AX4/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.321    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,611,326
Number of Sequences: 6
Number of extensions: 200731
Number of successful extensions: 1121
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 961
Number of HSP's gapped (non-prelim): 522
length of query: 257
length of database: 11,314,357
effective HSP length: 98
effective length of query: 159
effective length of database: 11,313,769
effective search space: 1798889271
effective search space used: 1798889271
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)