TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|166240590|ref|XP_643546.2| thyroxine 5'-deiodinase [Dictyostelium discoideum AX4] (257 letters) Database: C.fasciculata/genome.fa 1089 sequences; 37,048,325 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value Cf_Contig876 | | 1 to 197301 28 9.7 >Cf_Contig876 | | 1 to 197301 Length = 197301 Score = 27.7 bits (60), Expect = 9.7 Identities = 19/53 (35%), Positives = 26/53 (49%), Gaps = 4/53 (7%) Frame = +3 Query: 58 SRERLNLQADTQCPDAILLDDQTNQEVSLYDLFNSNNNEKSVQ----DRPMVL 106 SR RL ++ TQ AILLD SLY + ++Q DRP++L Sbjct: 12069 SRRRLPIEVSTQITKAILLDHYGGTSHSLYLFILIISFTSALQNLDKDRPLIL 12227 Database: C.fasciculata/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 37,048,325 Number of sequences in database: 1089 Lambda K H 0.321 0.140 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,076,907 Number of Sequences: 1089 Number of extensions: 137918 Number of successful extensions: 498 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 42 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 439 Number of HSP's gapped (non-prelim): 105 length of query: 257 length of database: 12,349,441 effective HSP length: 99 effective length of query: 158 effective length of database: 12,241,630 effective search space: 1934177540 effective search space used: 1934177540 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 60 (27.7 bits)