TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|29165348|gb|AAO65268.1| iodothyronine deiodinase type I
Deio1 [Danio rerio]
         (241 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig5077  12231 40311                                                48   3e-05
Contig19460  1960 7624                                                 36   0.14 
Contig7521  5445 6810                                                  35   0.23 
Contig9812  3307 4188                                                  32   3.4  
Contig1448  17924 22224                                                31   5.7  
Contig155789  1092 1092                                                30   7.5  
Contig59987  398 398                                                   30   7.5  
Contig15263  1674 1674                                                 30   7.5  
Contig946  23669 31464                                                 30   7.5  
Contig16058  2124 4421                                                 30   9.8  

>Contig5077  12231 40311
          Length = 40311

 Score = 48.1 bits (113), Expect = 3e-05
 Identities = 21/50 (42%), Positives = 32/50 (64%)
 Frame = +2

Query: 120  NRPLVLSFGSCTUPPFLYKLDEFKQLVKDFSNVADFLIVYLAEAHATDAW 169
            ++PLV+  GS + PP  + + +   L K+FS    F+ +Y+AEAHATD W
Sbjct: 4532 DKPLVIIGGSYS*PPMRFVVPQLHALQKEFSEKMSFVCIYIAEAHATDEW 4681


>Contig19460  1960 7624
          Length = 7624

 Score = 36.2 bits (82), Expect = 0.14
 Identities = 14/38 (36%), Positives = 26/38 (68%)
 Frame = -1

Query: 201  PVVVDEMNNITASKYGALPERLYVIQSGKVIYQASDLG 238
            P+VVD M+N     +GA P R +V+Q+G ++++  ++G
Sbjct: 7210 PMVVDTMSNQFDEHFGAWPVRFFVVQNGTLVFKVFNIG 7097


>Contig7521  5445 6810
          Length = 6810

 Score = 35.4 bits (80), Expect = 0.23
 Identities = 18/43 (41%), Positives = 25/43 (58%)
 Frame = -2

Query: 140 DEFKQLVKDFSNVADFLIVYLAEAHATDAWAFKNNVDISVHKN 182
           DE     K F++  DFL VY++EAHA+D W     V  + +KN
Sbjct: 440 DELICTQKSFASEVDFLGVYISEAHASDEWPLGIKVFKNKNKN 312


>Contig9812  3307 4188
          Length = 4188

 Score = 31.6 bits (70), Expect = 3.4
 Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
 Frame = +1

Query: 95   GHAAPDSALITLDRQKTSVHRFLKGNR--PLVLSFGSCTUPPF 135
            G A PD  L T     TS+H  L   R  P+V++ GS T PPF
Sbjct: 4027 GDAVPDVPLTTTRGCSTSLHSLLSPLRDVPIVIAAGSYT*PPF 4155


>Contig1448  17924 22224
          Length = 22224

 Score = 30.8 bits (68), Expect = 5.7
 Identities = 14/35 (40%), Positives = 22/35 (62%)
 Frame = -3

Query: 108   RQKTSVHRFLKGNRPLVLSFGSCTUPPFLYKLDEF 142
             R++  +   LK ++ LV  FG+CT PP L+ + EF
Sbjct: 13834 RREVGMLSRLKPHKHLVRYFGACTQPPHLFIITEF 13730


>Contig155789  1092 1092
          Length = 1092

 Score = 30.4 bits (67), Expect = 7.5
 Identities = 25/69 (36%), Positives = 32/69 (46%)
 Frame = -2

Query: 74  LAFIKTLFFVNWCSLGLEAFEGHAAPDSALITLDRQKTSVHRFLKGNRPLVLSFGSCTUP 133
           + F+ T F  N  SL +  F   +  +S+L  L  Q   V  FLKG  PL  +F SC   
Sbjct: 974 VVFV*T*FLFNHSSLIVLTFPSDS*SESSLFNL-LQFLYVVAFLKGLDPLPKNFSSCFPS 798

Query: 134 PFLYKLDEF 142
            FL  L  F
Sbjct: 797 FFLSFLSSF 771


>Contig59987  398 398
          Length = 398

 Score = 30.4 bits (67), Expect = 7.5
 Identities = 21/61 (34%), Positives = 26/61 (42%)
 Frame = +1

Query: 82  FVNWCSLGLEAFEGHAAPDSALITLDRQKTSVHRFLKGNRPLVLSFGSCTUPPFLYKLDE 141
           F  W + GL  +EG   P    +   R  T++ RFL       LSF S   PP L  L  
Sbjct: 4   FSLWDTCGLHVYEGSFLP----VVRSRGVTAIVRFLSPLSLSSLSFFSLLSPPLLSLLSS 171

Query: 142 F 142
           F
Sbjct: 172 F 174


>Contig15263  1674 1674
          Length = 1674

 Score = 30.4 bits (67), Expect = 7.5
 Identities = 17/43 (39%), Positives = 22/43 (51%)
 Frame = -1

Query: 41   GRMRKIHMKMGERSTMTQNPKFRYEDWGPAFFSLAFIKTLFFV 83
            G  RK H K+  ++T    P F  +DW   FF   F+K  FFV
Sbjct: 1467 GSFRKFHEKLEIQNTSF--PSFIGQDWSR*FFFAYFVKN*FFV 1345


>Contig946  23669 31464
          Length = 31464

 Score = 30.4 bits (67), Expect = 7.5
 Identities = 25/69 (36%), Positives = 32/69 (46%)
 Frame = -3

Query: 74   LAFIKTLFFVNWCSLGLEAFEGHAAPDSALITLDRQKTSVHRFLKGNRPLVLSFGSCTUP 133
            + F+ T F  N  SL +  F   +  +S+L  L  Q   V  FLKG  PL  +F SC   
Sbjct: 4693 VVFV*T*FLFNHSSLIVLTFPSDS*SESSLFNL-LQFLYVVAFLKGLDPLPKNFSSCFPS 4517

Query: 134  PFLYKLDEF 142
             FL  L  F
Sbjct: 4516 FFLSFLSSF 4490


>Contig16058  2124 4421
          Length = 4421

 Score = 30.0 bits (66), Expect = 9.8
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +1

Query: 201  PVVVDEMNNITASKYGALPERLYVIQSGKVIYQ 233
            PVVVD M N    ++GA P R +++ +  ++++
Sbjct: 3163 PVVVDTMKNEFNEQFGAWPARFFIVLNNTLVFK 3261


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.324    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,048,601
Number of Sequences: 297657
Number of extensions: 1330916
Number of successful extensions: 9001
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 8391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8997
length of query: 241
length of database: 106,339,350
effective HSP length: 110
effective length of query: 131
effective length of database: 73,597,080
effective search space: 9641217480
effective search space used: 9641217480
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 66 (30.0 bits)