TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|29165348|gb|AAO65268.1| iodothyronine deiodinase type I
Deio1 [Danio rerio]
         (241 letters)

Database: C.fasciculata/genome.fa 
           1089 sequences; 37,048,325 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Cf_Contig912  |  | 1 to 41032                                          31   1.0  
Cf_Contig894  |  | 1 to 240449                                         28   5.2  
Cf_Contig1039  |  | 1 to 222123                                        28   6.7  
Cf_Contig930  |  | 1 to 140823                                         28   8.8  

>Cf_Contig912  |  | 1 to 41032
          Length = 41032

 Score = 30.8 bits (68), Expect = 1.0
 Identities = 23/81 (28%), Positives = 35/81 (43%), Gaps = 3/81 (3%)
 Frame = +3

Query: 94   EGHAAPDSALITLDRQKTSVHRFLKGNRPLVLSFGSCTUPPF---LYKLDEFKQLVKDFS 150
            +GHAA    L T +    S HR  K       + G    P     + +   FK L+++ S
Sbjct: 2268 DGHAATTVTLQTSNTASASAHRQHKQGAAAGAAAGKADRPAMPAEVLRTRHFKDLIQNPS 2447

Query: 151  NVADFLIVYLAEAHATDAWAF 171
            +VA  L  Y+ ++ A D   F
Sbjct: 2448 SVARRLYRYVLQSFADDCRLF 2510


>Cf_Contig894  |  | 1 to 240449
          Length = 240449

 Score = 28.5 bits (62), Expect = 5.2
 Identities = 21/60 (35%), Positives = 31/60 (51%)
 Frame = -1

Query: 70    AFFSLAFIKTLFFVNWCSLGLEAFEGHAAPDSALITLDRQKTSVHRFLKGNRPLVLSFGS 129
             AFF+ +F  +LFF  + S  L A+ G   P  +L     ++   HR+L    PL L+F S
Sbjct: 96524 AFFTTSF--SLFFFCFPSPTLYAYIGVHIPTLSLFLRSAEQKGAHRYLV--TPLYLTFSS 96357


>Cf_Contig1039  |  | 1 to 222123
          Length = 222123

 Score = 28.1 bits (61), Expect = 6.7
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +1

Query: 63    RYEDWGPAFFSLAFIKTLFFVNWCSLGLEAFE 94
             + + W   FF L+F+ +LFF+  CSL   AF+
Sbjct: 59863 KLDRWRSFFFFLSFLFSLFFLLPCSLRYFAFD 59958


>Cf_Contig930  |  | 1 to 140823
          Length = 140823

 Score = 27.7 bits (60), Expect = 8.8
 Identities = 21/70 (30%), Positives = 29/70 (41%), Gaps = 11/70 (15%)
 Frame = -3

Query: 72    FSLAFIKTLFFVNWCSLGLEAFEGHAAPDSALIT-LDRQKTS----------VHRFLKGN 120
             F   F+  L FV   + GL  F  H    S + + +D++ T           +H  L G 
Sbjct: 74260 FFHVFVIILLFVAAAAAGLLQFASHRRSGSDVTSGMDKRATPSRTRGRS*VPLHTRLYGR 74081

Query: 121   RPLVLSFGSC 130
             RPL    GSC
Sbjct: 74080 RPLYFQVGSC 74051


  Database: C.fasciculata/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 37,048,325
  Number of sequences in database:  1089
  
Lambda     K      H
   0.324    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,450,746
Number of Sequences: 1089
Number of extensions: 138858
Number of successful extensions: 852
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 741
Number of HSP's gapped (non-prelim): 237
length of query: 241
length of database: 12,349,441
effective HSP length: 98
effective length of query: 143
effective length of database: 12,242,719
effective search space: 1750708817
effective search space used: 1750708817
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)