TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000003_1.0 # Protein # Iodothyronine deiodinase 3 (DI3) #
Homo sapiens # Complete
         (278 letters)

Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig5077 12231 40311                                                 36   0.17 
Contig19460 1960 7624                                                  33   1.4  
Contig48363 488 488                                                    32   2.5  
Contig26091 732 732                                                    31   5.5  
Contig11805 3283 9197                                                  31   5.5  
Contig253014 261 261                                                   31   7.2  
Contig16495 2190 2200                                                  31   7.2  
Contig7521 5445 6810                                                   31   7.2  

>Contig5077 12231 40311
          Length = 40311

 Score = 36.2 bits (82), Expect = 0.17
 Identities = 17/50 (34%), Positives = 27/50 (54%)
 Frame = +2

Query: 132  NRPLVLNFGSCTUPPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGW 181
            ++PLV+  GS + PP    +     L  ++   + F+ IYI EAH +D W
Sbjct: 4532 DKPLVIIGGSYS*PPMRFVVPQLHALQKEFSEKMSFVCIYIAEAHATDEW 4681


>Contig19460 1960 7624
          Length = 7624

 Score = 33.1 bits (74), Expect = 1.4
 Identities = 12/32 (37%), Positives = 24/32 (75%)
 Frame = -1

Query: 215  LVLDTMANSSSSAYGAYFERLYVIQSGTIMYQ 246
            +V+DTM+N     +GA+  R +V+Q+GT++++
Sbjct: 7207 MVVDTMSNQFDEHFGAWPVRFFVVQNGTLVFK 7112


>Contig48363 488 488
          Length = 488

 Score = 32.3 bits (72), Expect = 2.5
 Identities = 26/92 (28%), Positives = 41/92 (44%), Gaps = 6/92 (6%)
 Frame = -3

Query: 111 NSEVVLPDGFQSQ------HILDYAQGNRPLVLNFGSCTUPPFMARMSAFQRLVTKYQRD 164
           NS ++L   F SQ      +++ ++Q  +  V N   C    F      F R+   +Q  
Sbjct: 297 NSRILLIQFFFSQSQKCVNNLIFFSQYPK-CVKNLKCCNSRIFTNSKVLF-RMYEAFQEK 124

Query: 165 VDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSL 196
           V FL +YI EAH  D W   D   +  Q +++
Sbjct: 123 VKFLTVYILEAHTQDEWPIGDDVLLATQPKTV 28


>Contig26091 732 732
          Length = 732

 Score = 31.2 bits (69), Expect = 5.5
 Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 2/47 (4%)
 Frame = -1

Query: 113 EVVLPDGFQSQHILDYAQGNRPLVLNFGSCTUPPFM--ARMSAFQRL 157
           EV++  G ++ +++ Y     PLV+ +G+   PP++     SAF RL
Sbjct: 669 EVIVATGLRASNVIYYTHTRLPLVVAWGNLCVPPYLLYPLFSAFARL 529


>Contig11805 3283 9197
          Length = 9197

 Score = 31.2 bits (69), Expect = 5.5
 Identities = 13/23 (56%), Positives = 17/23 (73%)
 Frame = +1

Query: 47   GRRRRGQPEPEVELNSEGEEVPP 69
            GRR++G+ E EVE+  EGEE  P
Sbjct: 8593 GRRKKGREEEEVEVEGEGEEGTP 8661


>Contig253014 261 261
          Length = 261

 Score = 30.8 bits (68), Expect = 7.2
 Identities = 23/72 (31%), Positives = 30/72 (41%), Gaps = 2/72 (2%)
 Frame = -2

Query: 64  GEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQA-HEGGPA-PNSEVVLPDGFQ 121
           G  V    PP+C+   +      S+ A       D   Q+ HEG    P  E V+P G  
Sbjct: 248 GSSVKTTPPPVCLLTSHSASDSLSIVAANQFHCCDIGSQSLHEGHVIFPGEEDVIP-GML 72

Query: 122 SQHILDYAQGNR 133
            QH+LD   G R
Sbjct: 71  GQHLLDVPSGER 36


>Contig16495 2190 2200
          Length = 2200

 Score = 30.8 bits (68), Expect = 7.2
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +1

Query: 56   PEVELNSEGEEVPPDDPPICVSDDNRLCTLASLK 89
            P + +++    +    PP+CVS   RLCT ASL+
Sbjct: 1981 PSIPISASMRRLCAPPPPLCVSASLRLCTSASLR 2082


>Contig7521 5445 6810
          Length = 6810

 Score = 30.8 bits (68), Expect = 7.2
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = -2

Query: 161 YQRDVDFLIIYIEEAHPSDGW 181
           +  +VDFL +YI EAH SD W
Sbjct: 413 FASEVDFLGVYISEAHASDEW 351


  Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa
    Posted date:  Sep 30, 2011 12:44 AM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.322    0.139    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 124,857,935
Number of Sequences: 297657
Number of extensions: 2390754
Number of successful extensions: 10961
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 10549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10951
length of query: 278
length of database: 106,339,350
effective HSP length: 112
effective length of query: 166
effective length of database: 73,001,766
effective search space: 12118293156
effective search space used: 12118293156
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 67 (30.4 bits)