TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000003_1.0 # Protein # Iodothyronine deiodinase 3 (DI3) #
Homo sapiens # Complete
         (278 letters)

Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2, whole ...    94   9e-20
gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole ...    37   0.022

>gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2, whole genome shotgun sequence
          Length = 8484197

 Score = 94.4 bits (233), Expect = 9e-20
 Identities = 61/165 (36%), Positives = 89/165 (53%), Gaps = 5/165 (3%)
 Frame = +3

Query: 111     NSEVVLPDGFQSQHILDYAQGNRPLVLNFGSCTUPPFMARMSAFQRLVTKYQRDVDFLII 170
               N EV L D F S +     Q +RP+VL  GS T PPF  +M  FQ +   ++  VD  I+
Sbjct: 5884962 NQEVSLYDLFNSNNNEKSVQ-DRPMVLICGSFT*PPFRDKMCMFQDIFVDFKEWVDIYIV 5885138

Query: 171     YIEEAHPSDGWVT--TDSPYIIPQHRSLEDRVSAARVLQQGAPGCAL--VLDTMANSSSS 226
               Y++E HP+D W     +      Q +++EDR    + L++ AP C +  ++D M N+ + 
Sbjct: 5885139 YLKEIHPADEWYIGGDEISLCYRQPKTMEDRREIIKDLKEYAPFCTIPFLIDKMDNNFNK 5885318

Query: 227     AYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWL-ERYDEQL 270
                Y A  ERLYV++     Y GG GP G+   ELR +L +RY   L
Sbjct: 5885319 VYDAVPERLYVLEDKKFKYVGGPGPFGFIPEELREFLTKRYKPHL 5885453


>gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole genome shotgun sequence
          Length = 5125352

 Score = 36.6 bits (83), Expect = 0.022
 Identities = 33/95 (34%), Positives = 44/95 (46%), Gaps = 1/95 (1%)
 Frame = -1

Query: 44      HFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDD-NRLCTLASLKAVWHGQKLDFFKQ 102
               HF+ +   G  E +  L SE  E+P DD  I + ++   L  L S K  WH   LD FK+
Sbjct: 4274066 HFILKIEFGSGE-KYYLYSENSELPKDDVKIALGEECGSLLKLKSNKNFWHVILLDGFKE 4273890

Query: 103     AHEGGPAPNSEVVLPDGFQSQHILDYAQGNRPLVL 137
               + E G       VLP G +   I D      PLV+
Sbjct: 4273889 SLEPG-------VLPLGIEYLDIYDI---KTPLVI 4273815


  Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa
    Posted date:  Sep 29, 2011  8:31 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.322    0.139    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,190,122
Number of Sequences: 6
Number of extensions: 99755
Number of successful extensions: 346
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 310
Number of HSP's gapped (non-prelim): 60
length of query: 278
length of database: 11,314,357
effective HSP length: 99
effective length of query: 179
effective length of database: 11,313,763
effective search space: 2025163577
effective search space used: 2025163577
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)