TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000002_1.0 # Protein # Iodothyronine deiodinase 2 (DI2) # Homo sapiens # Complete (265 letters) Database: /users/rg/didac/GENOMES/I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL983107.1| Ichthyophthirius multifiliis unplaced genomic sca... 30 3.5 >gb|GL983107.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249536, whole genome shotgun sequence Length = 30310 Score = 29.6 bits (65), Expect = 3.5 Identities = 17/51 (33%), Positives = 25/51 (49%) Frame = -1 Query: 24 FLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQ 74 F+ L+ VILL+H+V L R+L + +WKSF+ Y Q Sbjct: 21007 FIVLFS*VILLRHIVRNLEMGNMRVFHLFRVLQNNSRWWLWKSFMKKNYCQ 20855 Database: /users/rg/didac/GENOMES/I.multifiliis_strain_G5/genome.fa Posted date: Sep 29, 2011 11:36 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,689,042 Number of Sequences: 2017 Number of extensions: 135446 Number of successful extensions: 957 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 118 Number of HSP's successfully gapped in prelim test: 25 Number of HSP's that attempted gapping in prelim test: 803 Number of HSP's gapped (non-prelim): 239 length of query: 265 length of database: 16,266,656 effective HSP length: 101 effective length of query: 164 effective length of database: 16,062,939 effective search space: 2634321996 effective search space used: 2634321996 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 61 (28.1 bits)