TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000001_1.0 # Protein # Iodothyronine deiodinase 1 (DI1) #
Homo sapiens # Complete
         (249 letters)

Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig5077 12231 40311                                                 42   0.003
Contig7521 5445 6810                                                   35   0.24 
Contig16058 2124 4421                                                  34   0.54 
Contig6299 7530 12275                                                  32   2.7  

>Contig5077 12231 40311
          Length = 40311

 Score = 41.6 bits (96), Expect = 0.003
 Identities = 21/50 (42%), Positives = 29/50 (58%)
 Frame = +2

Query: 114  NRPLVLNFGSCTUPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGW 163
            ++PLV+  GS + P   F   Q   L ++FS    F+ IYI EAHA+D W
Sbjct: 4532 DKPLVIIGGSYS*PPMRFVVPQLHALQKEFSEKMSFVCIYIAEAHATDEW 4681


>Contig7521 5445 6810
          Length = 6810

 Score = 35.4 bits (80), Expect = 0.24
 Identities = 33/121 (27%), Positives = 49/121 (40%), Gaps = 44/121 (36%)
 Frame = -2

Query: 141 EDFSSIADFLVIYIEEAHASDGW-----AFKN---NMDIRNH------------------ 174
           + F+S  DFL +YI EAHASD W      FKN   N  I N                   
Sbjct: 419 KSFASEVDFLGVYISEAHASDEWPLGIKVFKNKNKNNKIVNKK*GREGREREGEGVRFLT 240

Query: 175 -----------------QNLQDRLQAAHLLLARSP-QCPVVVDTMQNQSSQLYAALPERL 216
                            + ++ RL+ A+  +       P++VDTM+N+    +A+ PER 
Sbjct: 239 VCFLTFFVFF*YVYEQPKEMETRLKIANGFVNDFKFNLPMLVDTMENEFDSHFASWPERY 60

Query: 217 Y 217
           +
Sbjct: 59  F 57


>Contig16058 2124 4421
          Length = 4421

 Score = 34.3 bits (77), Expect = 0.54
 Identities = 13/33 (39%), Positives = 23/33 (69%)
 Frame = +1

Query: 195  PVVVDTMQNQSSQLYAALPERLYIIQEGRILYK 227
            PVVVDTM+N+ ++ + A P R +I+    +++K
Sbjct: 3163 PVVVDTMKNEFNEQFGAWPARFFIVLNNTLVFK 3261


>Contig6299 7530 12275
          Length = 12275

 Score = 32.0 bits (71), Expect = 2.7
 Identities = 14/36 (38%), Positives = 20/36 (55%)
 Frame = -3

Query: 53   NPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTEL 88
            +PHF   N++PTFF  + +      +WQR    TEL
Sbjct: 1806 HPHFCTHNFLPTFFLVKKYSKKKGQKWQRKNKATEL 1699


  Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa
    Posted date:  Sep 30, 2011 12:44 AM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.324    0.138    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,198,290
Number of Sequences: 297657
Number of extensions: 1585685
Number of successful extensions: 8132
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 7803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8130
length of query: 249
length of database: 106,339,350
effective HSP length: 111
effective length of query: 138
effective length of database: 73,299,423
effective search space: 10115320374
effective search space used: 10115320374
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 66 (30.0 bits)