TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000016_1.0 # Protein # Selenoprotein K (SelK) # Homo sapiens # Complete (94 letters) Database: P.capsici/genome.fa 917 sequences; 64,023,748 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PHYCAscaffold_2 45 2e-05 >PHYCAscaffold_2 Length = 1925921 Score = 45.1 bits (105), Expect = 2e-05 Identities = 19/38 (50%), Positives = 25/38 (65%) Frame = -2 Query: 1 MVYISNGQVLDSRSQSPWRLSLITDFFWGIAEFVVLFF 38 M Y+S G V+ RS PWRLS++TD WG+ FV L + Sbjct: 491284 MTYVSGGDVVAKRS--PWRLSIVTDMIWGVVNFVGLLY 491177 Database: P.capsici/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 64,023,748 Number of sequences in database: 917 Lambda K H 0.325 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,821,264 Number of Sequences: 917 Number of extensions: 44809 Number of successful extensions: 313 Number of sequences better than 1.0e-04: 1 Number of HSP's better than 0.0 without gapping: 35 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 253 Number of HSP's gapped (non-prelim): 107 length of query: 94 length of database: 21,341,249 effective HSP length: 69 effective length of query: 25 effective length of database: 21,277,976 effective search space: 531949400 effective search space used: 531949400 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 98 (42.4 bits)