TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000004_1.0 # Protein # Glutathione peroxidase 1 (GPx1) # Homo sapiens # Complete (203 letters) Database: D.fasciculatum/genome.fa 25 sequences; 31,019,208 total letters Searching.........................done Score E Sequences producing significant alignments: (bits) Value gi|328871378|gb|GL883013.1| Dictyostelium fasciculatum unplaced ... 28 3.3 >gi|328871378|gb|GL883013.1| Dictyostelium fasciculatum unplaced genomic scaffold Scf_ET2XIPL01CNAVH-3, whole genome shotgun sequence Length = 1908399 Score = 28.5 bits (62), Expect = 3.3 Identities = 15/37 (40%), Positives = 21/37 (56%) Frame = -3 Query: 30 VSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRL 66 VSLG +++I NV SL +T +DY +N Q L Sbjct: 1304209 VSLGIQMVNIMVITNVLSLQSSTNKDYCLLNIYQNML 1304099 Database: D.fasciculatum/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 31,019,208 Number of sequences in database: 25 Lambda K H 0.321 0.137 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,606,315 Number of Sequences: 25 Number of extensions: 72466 Number of successful extensions: 223 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 219 Number of HSP's gapped (non-prelim): 16 length of query: 203 length of database: 10,339,736 effective HSP length: 95 effective length of query: 108 effective length of database: 10,337,361 effective search space: 1116434988 effective search space used: 1116434988 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 58 (26.9 bits)