TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|298566227|ref|NP_001177288.1| disulfide bond formation
protein A [Ciona intestinalis]
         (214 letters)

Database: C.fasciculata/genome.fa 
           1089 sequences; 37,048,325 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Cf_Contig876  |  | 1 to 197301                                         61   8e-10
Cf_Contig932  |  | 1 to 156640                                         30   1.1  
Cf_Contig911  |  | 1 to 78501                                          29   2.5  
Cf_Contig970  |  | 1 to 287441                                         28   4.2  
Cf_Contig1069  |  | 1 to 371363                                        27   9.4  

>Cf_Contig876  |  | 1 to 197301
          Length = 197301

 Score = 60.8 bits (146), Expect = 8e-10
 Identities = 53/233 (22%), Positives = 100/233 (42%), Gaps = 26/233 (11%)
 Frame = +1

Query: 2     SKFLHIRVVSDIMXPWCWVGKRNLETA---MKAS--ENEYKFKVTWEPFLLRPNMPL--- 53
             +K + + V SD+  PWCWVGKR +E A   + AS  ++ ++ ++ W PF L P +     
Sbjct: 60376 AKKVVVNVTSDVACPWCWVGKRAIEVAAANLNASHPDSPFELELHWLPFQLDPTIQSGTT 60555

Query: 54    -------------EGIAKQGDFGPHTPGAQRLINVGRKVGVEFAFKQPRYPYTLF-GHCA 99
                            +A+Q    P T    R     +   +EF        ++ +  H  
Sbjct: 60556 HNLLDHLSAMFGNPRLAEQWVSNPETIPMNRSCVASKLPNIEFRMTAQAVRFSTYRAHTL 60735

Query: 100   LEFAIKKDPSGSIQTQLQESLFKSYFTDGEYPD-VETVSTVAATCGL--NREEVKSFISD 156
             L      + + S Q +L+E++ +    +G+  D ++ ++  A   G+   ++EV+  + +
Sbjct: 60736 LTHTGPMEKNWSAQNRLKEAMLRMTHKEGKNLDSLDALTAAAQEAGIAQTQQEVEQLLGN 60915

Query: 157   ESNLAAVKRKAAQWS-ANGVSGVPYFIINDCPVFSGAQEPAAFQNIFAKVAEK 208
                 A + ++  Q       +GVP+F   +   FSG Q    F+    +V  K
Sbjct: 60916 ADVTAVLDKELRQSRLLPHFNGVPHFTFPNGKSFSGGQPVEVFEAALEEVLTK 61074


>Cf_Contig932  |  | 1 to 156640
          Length = 156640

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 18/57 (31%), Positives = 29/57 (50%)
 Frame = +2

Query: 44    PFLLRPNMPLEGIAKQGDFGPHTPGAQRLINVGRKVGVEFAFKQPRYPYTLFGHCAL 100
             PF+L   + +    ++GD  P+ P  + +INV    G  + FKQ  +P+T     AL
Sbjct: 49538 PFILNGKLKVCLTDREGDAVPNEP--RFIINVSAMEGQFYRFKQTTHPHTNMAKAAL 49702


>Cf_Contig911  |  | 1 to 78501
          Length = 78501

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
 Frame = -2

Query: 127   DGEYPDVETVSTVAATCGL-NREEVKSFISDESNLAAVKRKAAQWSANGVSGVPYFII 183
             DGE  ++   S +AA C    R    +F S     A    +++    + VSG+PY  I
Sbjct: 17732 DGEEEELSASSLLAAACSTPERSNATAFASSPDTAALPTSESSLLQTSPVSGIPYATI 17559


>Cf_Contig970  |  | 1 to 287441
          Length = 287441

 Score = 28.5 bits (62), Expect = 4.2
 Identities = 29/103 (28%), Positives = 42/103 (40%), Gaps = 5/103 (4%)
 Frame = +2

Query: 105   KKDPSGSIQTQLQE----SLFKSYFTDGEYPDVETVSTVAATCGLNREEVKSFISDESNL 160
             +++  GS+   +Q      L  SY +    P    ++T  A CGL       FI+DES  
Sbjct: 54515 REELKGSLTAYVQSVEAGQLISSYDSPFLSPFDTLLATEPALCGLLEALPSLFIADESFW 54694

Query: 161   A-AVKRKAAQWSANGVSGVPYFIINDCPVFSGAQEPAAFQNIF 202
             A  VK    +W A    G      ++ P  +G Q  A  Q  F
Sbjct: 54695 ATTVKPAVERWCAAVRGGGKCVEEHEAPQHNGMQWAAQLQLSF 54823


>Cf_Contig1069  |  | 1 to 371363
          Length = 371363

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = -1

Query: 64     PHTPGAQRLINVGRKVGVEFAFKQPRYPYTLFGHCA 99
              PH PG ++L++ G  +GV  A    R P  L G  A
Sbjct: 105278 PHVPGPRQLLHRGLVLGVAAAGPAQRRPQLLRGDAA 105171


  Database: C.fasciculata/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 37,048,325
  Number of sequences in database:  1089
  
Lambda     K      H
   0.319    0.134    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,770,652
Number of Sequences: 1089
Number of extensions: 133079
Number of successful extensions: 548
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 432
Number of HSP's gapped (non-prelim): 205
length of query: 214
length of database: 12,349,441
effective HSP length: 97
effective length of query: 117
effective length of database: 12,243,808
effective search space: 1432525536
effective search space used: 1432525536
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)