TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|74761781|sp|Q9NX07.1|TSAP1_HUMAN RecName: Full=tRNA
selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName:
Full=tRNA selenocysteine-associated protein 1
         (287 letters)

Database: L.donovani_BPK282A1/genome.fa 
           36 sequences; 32,444,998 total letters

Searching....................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

contig_35                                                              76   3e-14
contig_25                                                              59   5e-09

>contig_35
          Length = 2113966

 Score = 75.9 bits (185), Expect = 3e-14
 Identities = 55/168 (32%), Positives = 90/168 (53%), Gaps = 4/168 (2%)
 Frame = +3

Query: 3       ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62
               AS+++GDL+  ++E  +   F   G T+++V++ R+ +T    GY +V F +  +AEK +
Sbjct: 2082417 ASIYVGDLDAAINEPQLVELFKPFG-TILNVRVCRDIITQRSLGYGYVNFDNHDSAEKAI 2082593

Query: 63      HKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYS----LFVGDLTPDVDDGMLYEFFVK 118
                 +N K +      K  +L +    +Q D +  YS    +FV +L  DVD   L++ F K
Sbjct: 2082594 ESMNFKRVGD----KCVRLMW----QQRDPALRYSGNGNVFVKNLEKDVDSKSLHDIFTK 2082749

Query: 119     VYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSK 166
                + S    KV+ D+ G S+GYGFV F DE   K A+ +  GA    S+
Sbjct: 2082750 -FGSILSCKVMQDEEGKSRGYGFVHFKDETSAKDAIVKMNGAADHASE 2082890



 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 31/87 (35%), Positives = 47/87 (54%), Gaps = 3/87 (3%)
 Frame = +1

Query: 88      KQPD---NSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKF 144
               KQP      P  S++VG L PD+ +  LY++FV++ P       V   T  S GYG+V F
Sbjct: 1655728 KQPTMAFTGPNPSIWVGGLDPDLQEQKLYDYFVRIGPVTSVRVCVDSATQKSLGYGYVNF 1655907

Query: 145     TDELEQKRALTECQGAVGLGSKPVRLS 171
                D  + ++AL   Q    LGS+ +R++
Sbjct: 1655908 QDPADAEKALD--QAGSKLGSRYLRIA 1655982



 Score = 49.3 bits (116), Expect = 3e-06
 Identities = 46/158 (29%), Positives = 68/158 (43%), Gaps = 2/158 (1%)
 Frame = +1

Query: 4       SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63
               S+W+G L+P + E  +   F  +G  V SV++  +  T    GY +V F D A AEK L 
Sbjct: 1655764 SIWVGGLDPDLQEQKLYDYFVRIG-PVTSVRVCVDSATQKSLGYGYVNFQDPADAEKALD 1655940

Query: 64      KINGKPLPGATPAKRFKLNYATYGKQPD--NSPEYSLFVGDLTPDVDDGMLYEFFVKVYP 121
               +       G+    R+ L  A   + P    S   ++ V  L   VD   L E F K + 
Sbjct: 1655941 Q------AGSKLGSRY-LRIAKIQRDPSKRRSGVNNILVKKLPKSVDTYALKEMFSK-FG 1656096

Query: 122     SCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQG 159
                     +  D+ G S+GY  + F  E     A+ E  G
Sbjct: 1656097 RLTAIGLACDEKGESRGYARISFEREESAVDAVREMDG 1656210



 Score = 47.8 bits (112), Expect = 1e-05
 Identities = 48/195 (24%), Positives = 89/195 (45%), Gaps = 14/195 (7%)
 Frame = +1

Query: 4       SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63
               +L++ +L+P + +  +   FA  GE V S K+         AG  +V F     A + + 
Sbjct: 1656289 NLYVKNLDPAVTDEKLRAFFAKYGE-VSSAKVRDLGAVQSEAGLGYVAFQKHEDAARAVE 1656465

Query: 64      KINGK---------PLPGATPAKRFKLNYATYGKQPDNSPEYS----LFVGDLTPDVDDG 110
               ++NGK         PL  +    R +       ++ + + ++S    L+V      V   
Sbjct: 1656466 ELNGKECEISKAGSPLDVSRFRSREERQRDRERQRRERAQQHSKYPNLYVKGFDDTVTSE 1656645

Query: 111     MLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVR 169
                L E F + Y       V++D +TGVS+ +GFV   D+    +A+ E  G+  L  +P+ 
Sbjct: 1656646 RLEELFQR-YGETVSVTVMMDKETGVSRCFGFVSMKDQNAASQAIQELNGSTFLSPRPLF 1656822

Query: 170     LSVAIPKASRVKPVE 184
               ++ A+ K +R + +E
Sbjct: 1656823 VTYALRKDARRQNLE 1656867


>contig_25
          Length = 897883

 Score = 58.5 bits (140), Expect = 5e-09
 Identities = 54/180 (30%), Positives = 86/180 (47%), Gaps = 4/180 (2%)
 Frame = -1

Query: 4     SLWMGDLEPYMD--ENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61
             S+++GDL   +   E  I+  F+T+   V+SVK+ R+  T    GY +V F   A AEK 
Sbjct: 21100 SVYVGDLPIDLPRPEEAINNLFSTVAP-VVSVKVCRDMATQRSLGYGYVNFQTTADAEKV 20924

Query: 62    LHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKV-- 119
             +  +N     G  P ++ ++ ++        S   ++FV  L   ++   L   F K   
Sbjct: 20923 IDALN---YTGIAPGRQIRVMFSIRDPLQRKSGMNNVFVKKLDTAINAKELQAAFTKCGR 20753

Query: 120   YPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179
               SC   KV LD  G SKGYGFV+F +  E  +A  +  G+  LG   V ++  + +  R
Sbjct: 20752 VLSC---KVALDSAGNSKGYGFVQF-ETAEGAKAALDMNGS-KLGDSEVVVAPFVRRVDR 20588


  Database: L.donovani_BPK282A1/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 32,444,998
  Number of sequences in database:  36
  
Lambda     K      H
   0.317    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,977,511
Number of Sequences: 36
Number of extensions: 111335
Number of successful extensions: 487
Number of sequences better than 1.0e-04: 2
Number of HSP's better than  0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 421
Number of HSP's gapped (non-prelim): 117
length of query: 287
length of database: 10,814,999
effective HSP length: 99
effective length of query: 188
effective length of database: 10,811,435
effective search space: 2032549780
effective search space used: 2032549780
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 103 (44.3 bits)