TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000052_1.0 # Protein # Selenoprotein H1 (SelH1) # Anopheles gambiae # Complete (131 letters) Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa 6 sequences; 33,943,072 total letters Searching......done Score E Sequences producing significant alignments: (bits) Value gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole ... 28 1.8 >gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole genome shotgun sequence Length = 5125352 Score = 28.1 bits (61), Expect = 1.8 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = +2 Query: 90 IAKGKDGEKVLVWSGLPKGPPRKDKFPEPKTIL 122 + K K +K LVW+ GP + + FP PK IL Sbjct: 3513563 LGKKKKKKKNLVWAPRFLGPKKLNFFPTPKKIL 3513661 Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa Posted date: Sep 29, 2011 8:31 PM Number of letters in database: 33,943,072 Number of sequences in database: 6 Lambda K H 0.315 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 2,893,857 Number of Sequences: 6 Number of extensions: 27703 Number of successful extensions: 55 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 52 Number of HSP's gapped (non-prelim): 3 length of query: 131 length of database: 11,314,357 effective HSP length: 89 effective length of query: 42 effective length of database: 11,313,823 effective search space: 475180566 effective search space used: 475180566 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 55 (25.8 bits)