TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|323448484|gb|EGB04382.1| selenoprotein [Aureococcus anophagefferens] (113 letters) Database: P.polycephalum/genome.fa 297,657 sequences; 319,018,052 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Contig6920 5927 5957 28 7.8 >Contig6920 5927 5957 Length = 5957 Score = 28.5 bits (62), Expect = 7.8 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = -1 Query: 29 EGGVVVVEACKSUGAFKTRANKVLKALEGKAEVKINEEKPRKGAFVVSV 77 EGG ++E C K + + +EG ++K+ + + ++G +V V Sbjct: 2348 EGGDKLIELCSKGNIDKRLRERGNREVEGSTKIKVGQSRGKRGNRLVEV 2202 Database: P.polycephalum/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 319,018,052 Number of sequences in database: 297,657 Lambda K H 0.311 0.127 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,638,990 Number of Sequences: 297657 Number of extensions: 171780 Number of successful extensions: 487 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 479 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 487 length of query: 113 length of database: 106,339,350 effective HSP length: 81 effective length of query: 32 effective length of database: 82,229,133 effective search space: 2631332256 effective search space used: 2631332256 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 61 (28.1 bits)