TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|33285002|ref|NP_060915.2| selenoprotein S [Homo sapiens]
         (189 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501382|gb|GL984282.1| Ichthyophthirius multifiliis unplace...    35   0.047
gi|340499430|gb|GL984923.1| Ichthyophthirius multifiliis unplace...    33   0.23 
gi|340507794|gb|GL983394.1| Ichthyophthirius multifiliis unplace...    31   0.88 
gi|340501650|gb|GL984247.1| Ichthyophthirius multifiliis unplace...    29   3.3  
gi|340503888|gb|GL983999.1| Ichthyophthirius multifiliis unplace...    28   4.4  
gi|340505964|gb|GL983749.1| Ichthyophthirius multifiliis unplace...    28   4.4  
gi|340506843|gb|GL983551.1| Ichthyophthirius multifiliis unplace...    28   5.7  
gi|340506985|gb|GL983521.1| Ichthyophthirius multifiliis unplace...    28   5.7  
gi|340505486|gb|GL983812.1| Ichthyophthirius multifiliis unplace...    28   7.4  
gi|340507744|gb|GL983410.1| Ichthyophthirius multifiliis unplace...    27   9.7  

>gi|340501382|gb|GL984282.1| Ichthyophthirius multifiliis unplaced genomic
              scaffold scaff_1120509251321, whole genome shotgun sequence
          Length = 303348

 Score = 35.0 bits (79), Expect = 0.047
 Identities = 20/76 (26%), Positives = 38/76 (50%)
 Frame = +1

Query: 77     KRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQ 136
              KR++     + + +EE   + EK KEK K+ EEE+  +K       ++ K  K   KK +
Sbjct: 211435 KRRKRRKRRKRRKEEEEENRKEKEKEKEKEEEEEEEEEKKNKKKKKKKNKKKKKKKKKKK 211614

Query: 137    EEDSPGPSTSSVLKRK 152
              ++        +++K+K
Sbjct: 211615 KK*KKKKKKKTMMKKK 211662



 Score = 33.9 bits (76), Expect = 0.10
 Identities = 23/86 (26%), Positives = 42/86 (48%)
 Frame = +2

Query: 71     EPDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKG 130
              E ++  +++E     + + +EE   + EK KEK K+ EEE+  +K +  +  +E K  K 
Sbjct: 211271 EINIEEEKKEEEKEEKEEEEEEEENEKEKEKEKEKEKEEEEEEEKKKEEEKKEEEKEEK- 211447

Query: 131    NAKKPQEEDSPGPSTSSVLKRKSDRK 156
                K+ +EE           K+K  +K
Sbjct: 211448 EEKEEKEEKKKKKKIEKKKKKKRKKK 211525



 Score = 30.4 bits (67), Expect = 1.1
 Identities = 21/72 (29%), Positives = 34/72 (47%), Gaps = 2/72 (2%)
 Frame = +2

Query: 90     QEELNAQVEKHKEKLKQLEEEKRR--QKIEMWDSMQEGKSYKGNAKKPQEEDSPGPSTSS 147
              + E   + EK KEK ++ EEEK++  +K E     +E K  K   KK ++ +        
Sbjct: 211340 ENEKEKEKEKEKEKEEEEEEEKKKEEEKKEEEKEEKEEKEEKEEKKKKKKIEKKKKKKRK 211519

Query: 148    VLKRKSDRKPLR 159
                K+K  +K  R
Sbjct: 211520 KKKKKKKKKKKR 211555



 Score = 29.3 bits (64), Expect = 2.6
 Identities = 19/83 (22%), Positives = 37/83 (44%)
 Frame = +1

Query: 77     KRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQ 136
              +R+      R K ++E   +  K KEK K+ EEE+  ++ +     ++ K+ K   KK +
Sbjct: 211429 RRKRRKRRKRRKRRKEEEEENRKEKEKEKEKEEEEEEEEEKKNKKKKKKKNKKKKKKKKK 211608

Query: 137    EEDSPGPSTSSVLKRKSDRKPLR 159
              ++             K  RK ++
Sbjct: 211609 KKKK*KKKKKKKTMMKKKRKKIK 211677



 Score = 28.5 bits (62), Expect = 4.4
 Identities = 18/74 (24%), Positives = 35/74 (47%), Gaps = 9/74 (12%)
 Frame = +2

Query: 91     EELNAQVEKHKEKLKQLEE---------EKRRQKIEMWDSMQEGKSYKGNAKKPQEEDSP 141
              EE+N + EK +E+ ++ EE         EK ++K +  +  +E +  K   KK +E++  
Sbjct: 211268 EEINIEEEKKEEEKEEKEEEEEEEENEKEKEKEKEKEKEEEEEEEKKKEEEKKEEEKEEK 211447

Query: 142    GPSTSSVLKRKSDR 155
                      K+K  +
Sbjct: 211448 EEKEEKEEKKKKKK 211489


>gi|340499430|gb|GL984923.1| Ichthyophthirius multifiliis unplaced
           genomic scaffold scaff_5495, whole genome shotgun
           sequence
          Length = 1353

 Score = 32.7 bits (73), Expect = 0.23
 Identities = 15/51 (29%), Positives = 31/51 (60%)
 Frame = -3

Query: 88  KMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEE 138
           +++ E   ++EK KEK K+ E+EK ++K +  +  +E +  K   K+ ++E
Sbjct: 649 EIENEKENEIEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKEKE 497


>gi|340507794|gb|GL983394.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509249958, whole genome shotgun
             sequence
          Length = 43560

 Score = 30.8 bits (68), Expect = 0.88
 Identities = 15/42 (35%), Positives = 28/42 (66%)
 Frame = +3

Query: 72    PDVVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRR 113
             PD++  +QE +     K + E N ++ KHKE++K+ +EEK++
Sbjct: 28506 PDII--KQENIK----KKENEKNQELNKHKEEIKKNKEEKKK 28613


>gi|340501650|gb|GL984247.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251286, whole genome shotgun
             sequence
          Length = 122534

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 19/78 (24%), Positives = 44/78 (56%), Gaps = 6/78 (7%)
 Frame = -2

Query: 88    KMQEELNAQVEKHKEKLKQLEE--EKRRQKIEMWDSMQEGKSYKGNAKKPQE----EDSP 141
             K +E+     + +KEK K  ++  EK+++K ++ D  ++GKS K   +K +     ++  
Sbjct: 38170 KKKEDKKKNKKNYKEKKKNKKDLKEKKKKKKDLKD--KKGKSKKDLKEKKKNKKDLKEKK 37997

Query: 142   GPSTSSVLKRKSDRKPLR 159
             G +   ++++K ++K L+
Sbjct: 37996 GKNKKCLIEKKKNKKDLK 37943


>gi|340503888|gb|GL983999.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251036, whole genome shotgun
             sequence
          Length = 50548

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 10/51 (19%)
 Frame = -1

Query: 77    KRQEALAAARLKMQEELNAQVEKHKE----------KLKQLEEEKRRQKIE 117
             +++E     R K +EE   + E+ KE          K++Q EEE++RQK E
Sbjct: 37597 RKEEEEEEMRQKEEEEKRKEQERRKEEEEMRLK*EEKMRQKEEEEKRQKEE 37445



 Score = 27.3 bits (59), Expect = 9.7
 Identities = 18/69 (26%), Positives = 34/69 (49%)
 Frame = -3

Query: 88    KMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEEDSPGPSTSS 147
             K QEE   + ++ +EK ++ EEEK R+K E     +E +  +   ++ +EE+        
Sbjct: 38582 KKQEEKEKKEKEEEEKREKEEEEK*REKEE-----KERREIEEKERREKEEEREKEKEEK 38418

Query: 148   VLKRKSDRK 156
               K K + +
Sbjct: 38417 *KKEKEEEE 38391


>gi|340505964|gb|GL983749.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250725, whole genome shotgun
             sequence
          Length = 58153

 Score = 28.5 bits (62), Expect = 4.4
 Identities = 16/60 (26%), Positives = 35/60 (58%)
 Frame = +1

Query: 74    VVVKRQEALAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAK 133
             + +K+ +     + KM+++L  ++ KHK+K KQ ++ K RQK +  +  ++ + +K   K
Sbjct: 12508 IKIKK*KLKLKLKHKMKQKLKQKL-KHKQKQKQNKKNKLRQKKK*KNKQKQKQKHKNKHK 12684


>gi|340506843|gb|GL983551.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250345, whole genome shotgun
             sequence
          Length = 63840

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 1/57 (1%)
 Frame = +1

Query: 82    LAAARLKMQEELNAQVEKHKEKLKQLEEEKRRQK-IEMWDSMQEGKSYKGNAKKPQE 137
             LAA RLK  EE   +  K +++ K+ EEE+ R + +E+    +E K  K   K+ +E
Sbjct: 45430 LAAERLKKIEEEKLEQ*KLEDE*KR*EEEEERMR*LEISKQEEEKKKKKDEKKRLKE 45600


>gi|340506985|gb|GL983521.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509250296, whole genome shotgun
             sequence
          Length = 79067

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 18/51 (35%), Positives = 28/51 (54%)
 Frame = +1

Query: 88    KMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQEGKSYKGNAKKPQEE 138
             K QEE   Q EK++EK ++ +EEK  +  E     ++ K  +   K+ QEE
Sbjct: 46576 KKQEE--NQEEKYEEKQEENKEEK*EENQEEKQKKKQEKKQEEKQKEKQEE 46722


>gi|340505486|gb|GL983812.1| Ichthyophthirius multifiliis unplaced genomic
              scaffold scaff_1120509250848, whole genome shotgun sequence
          Length = 184484

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 20/64 (31%), Positives = 34/64 (53%), Gaps = 3/64 (4%)
 Frame = -3

Query: 77     KRQEALAAARLKMQEELNAQ--VEKHKEKLKQLEEEKRRQ-KIEMWDSMQEGKSYKGNAK 133
              K++E     + + +EE N +   EK+KEK     EEK+ + K E  +  +E K  + N +
Sbjct: 121119 KKEEKKEEKKEEKKEEKNEENLEEKNKEKKGDKNEEKKEEKKEEKKEEKKEEKKEEKNEE 120940

Query: 134    KPQE 137
              K +E
Sbjct: 120939 KNKE 120928


>gi|340507744|gb|GL983410.1| Ichthyophthirius multifiliis unplaced
            genomic scaffold scaff_1120509250066, whole genome
            shotgun sequence
          Length = 28596

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 19/76 (25%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
 Frame = +3

Query: 86   RLKMQEELNAQVEKHKEKLKQLEEEKRRQKIEMWDSMQ-----EGKSYKGNAKKPQEEDS 140
            +++ +EE   +    K+ +++  EEK+ +KIE  +  +     E K  K   KK +++D 
Sbjct: 8313 KVEKKEEKK*EKTIEKKTIEKKREEKKHEKIEKKNEKKQENTIEKKEIKKEVKKDEKKDE 8492

Query: 141  PGPSTSSVLKRKSDRK 156
                     K+K D+K
Sbjct: 8493 KKDD-----KKKDDKK 8525


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.313    0.130    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,503,515
Number of Sequences: 2017
Number of extensions: 53674
Number of successful extensions: 527
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 325
Number of HSP's gapped (non-prelim): 252
length of query: 189
length of database: 16,266,656
effective HSP length: 97
effective length of query: 92
effective length of database: 16,071,007
effective search space: 1478532644
effective search space used: 1478532644
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)