TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000017_1.0 # Protein # Selenoprotein M (SelM) # Homo sapiens # Complete (145 letters) Database: P.polycephalum/genome.fa 297,657 sequences; 319,018,052 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Contig39280 635 635 32 0.67 >Contig39280 635 635 Length = 635 Score = 32.3 bits (72), Expect = 0.67 Identities = 23/61 (37%), Positives = 31/61 (50%), Gaps = 17/61 (27%) Frame = -1 Query: 1 MSLLLPPLALLLLLAALVAPA-----------------TAATAYRPDWNRLSGLTRARVE 43 +S LL PL LLL L +L++P T+AT +R + NRLS RAR+ Sbjct: 230 LSSLLSPLFLLLFLFSLLSPLSSLAYVFPARKACDDWLTSATRFRLEVNRLSHAFRARIA 51 Query: 44 T 44 T Sbjct: 50 T 48 Database: P.polycephalum/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 319,018,052 Number of sequences in database: 297,657 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 59,850,234 Number of Sequences: 297657 Number of extensions: 1038964 Number of successful extensions: 4797 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 4545 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4783 length of query: 145 length of database: 106,339,350 effective HSP length: 102 effective length of query: 43 effective length of database: 75,978,336 effective search space: 3267068448 effective search space used: 3267068448 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 62 (28.5 bits)