TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|288565202|gb|GG743995.1|:subseq(66495,30000) Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.113, whole genome shotgun sequence:[translate(1)] (474 letters) Database: P.capsici/genome.fa 917 sequences; 64,023,748 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PHYCAscaffold_84 628 e-180 PHYCAscaffold_8 226 6e-59 PHYCAscaffold_51 127 3e-29 PHYCAscaffold_20 106 9e-23 >PHYCAscaffold_84 Length = 182275 Score = 628 bits (1619), Expect = e-180 Identities = 320/494 (64%), Positives = 379/494 (76%), Gaps = 21/494 (4%) Frame = -3 Query: 2 YDWDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLM 61 YD+DLVVIGGGSGGLA SKEAA +GQKV VLDYVKPSPQG++WGLGGTCVNVGCIPKKLM Sbjct: 97736 YDYDLVVIGGGSGGLACSKEAASFGQKVCVLDYVKPSPQGTSWGLGGTCVNVGCIPKKLM 97557 Query: 62 HQSAIMGELLHKDAEAFGWAVPNA-----TFDWTKLVTSVQDYIHGLNFKYRVDLRDKKV 116 HQS+++GE++H D FGW + + TF+W +LV++V YI +NFKY+V+LR K V Sbjct: 97556 HQSSLIGEVIHHDGANFGWNLSSGDGNAPTFNWKQLVSNVDGYIKSINFKYKVELRSKYV 97377 Query: 117 KYENFLGTFKDAHTLTLTNPAKGKPPKDVTFRRALIAVGGRPKSLDCPGADLAISSDDIF 176 KYENFLG+F + HTL L +P KG K +T R +IAVGGRPK L CPG + AISSDDIF Sbjct: 97376 KYENFLGSFVNPHTLELWHPRKGT--KQITTRDVVIAVGGRPKELTCPGGEHAISSDDIF 97203 Query: 177 -SRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVRSILLRGFDTDMAARIGTYMEEE 235 + PGKTLVVGASYVALECAGFLK G+DV VMVRSILLRGFD DMA +IG YME + Sbjct: 97202 WMKKKEPGKTLVVGASYVALECAGFLKGMGYDVKVMVRSILLRGFDQDMADKIGEYMEVQ 97023 Query: 236 AKIDFIKGAVPTSIEQLANG--------------EGIYDTVLNATGRNPDVSGLNLPAAG 281 + I+FI+ VP SI +L NG E +DTVLNATGR+PDV+ LNL AG Sbjct: 97022 SGIEFIRKTVPQSITKLENGQLLVKWTSEDGESCEEAFDTVLNATGRDPDVAKLNLDKAG 96843 Query: 282 VGLNPKTGRIAVTHEQTSAPHIYAVGDVIDGPELTPVAIQAGRLLAKRLFDNSATLMAYD 341 V LN K+GRI V +EQTS P+IYA+GDVID PELTPVAIQAGRLL++RL++NS M Y+ Sbjct: 96842 VKLNEKSGRIWVKNEQTSTPNIYAIGDVIDAPELTPVAIQAGRLLSRRLYNNSTVQMDYE 96663 Query: 342 KVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHD-RALAKECYCKL 400 KVCTAVFTPIEYGCCG+SE+ + E G DN+ VYH +F PLEWSLS D R A+ CY K+ Sbjct: 96662 KVCTAVFTPIEYGCCGMSEEDSKERFGEDNIEVYHTNFVPLEWSLSTDTRTAAESCYLKV 96483 Query: 401 IVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIFTTLEIT 460 I D+T++ VVGFHYLGPNAGEVTQA+G+AMKL +YD V TVGIHPTTAE FTTLE+T Sbjct: 96482 ICDKTRDKFVVGFHYLGPNAGEVTQAMGLAMKLGFTYDQMVDTVGIHPTTAESFTTLEVT 96303 Query: 461 KESGLDASAAGCUG 474 K SG + GC G Sbjct: 96302 KSSGGATTGGGC*G 96261 >PHYCAscaffold_8 Length = 1194346 Score = 226 bits (576), Expect = 6e-59 Identities = 164/479 (34%), Positives = 235/479 (49%), Gaps = 25/479 (5%) Frame = +2 Query: 4 WDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLMHQ 63 +D +VIGGGSGG+A+++ AA YG KVLV V+ + GLGGTCVN GC+PKK+M Sbjct: 676628 YDYMVIGGGSGGMASARRAATYGAKVLV---VERGREFDGMGLGGTCVNFGCVPKKVMFN 676798 Query: 64 SAIMGELLHKDAEAFGWAVPNAT-------FDWTKLVTSVQDYIHGLNFKYRVDLRDKKV 116 +A E L+++ + +++ A FDW + YI LN Y +L + V Sbjct: 676799 TAAHVEHLNRNKD---YSINTADKEFQFGDFDWNNMKIKRDAYIKRLNGIYERNLGNANV 676969 Query: 117 KYENFLGTFKDAHTLTLTNPAKGKPPKDVTFRRALIAVGGRPKSLDCPGADLAISSDDIF 176 + + + + + K T + L+A GG P D PG + I SD F Sbjct: 676970 DHVQGIAKLVAKDKIQVGD-------KVYTGKHVLVAPGGVPSVPDLPGMENVIDSDGFF 677128 Query: 177 SRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVR-SILLRGFDTDMAARIGTYMEEE 235 P K VVGA Y+A+E AG D T+ R +LR FD + + M E+ Sbjct: 677129 KLEQQPKKVAVVGAGYIAVEMAGIFNVLKSDTTLFCRFDQVLRKFDPIVRDLVNEEM-EK 677305 Query: 236 AKIDFIKGAVPTSIEQLANGE--------------GIYDTVLNATGRNPDVSGLNLPAAG 281 A + F++ +E+ NG+ +DT+L A GR P L L G Sbjct: 677306 AGVKFVRNMRAARVEKQDNGKLTFVVTINGEEQKFPDFDTILYAVGREPRTKDLGLEEVG 677485 Query: 282 VGLNPKTGRIAV-THEQTSAPHIYAVGD-VIDGPELTPVAIQAGRLLAKRLFDNSA-TLM 338 V L+ + G I V E TS P +YA+GD + G ELTPVAI AGR L+ RLF + Sbjct: 677486 VKLS-EGGFIEVDAQENTSVPGVYAIGDATVTGWELTPVAIAAGRRLSDRLFGGEKDACL 677662 Query: 339 AYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHDRALAKECYC 398 Y ++ T +F+ G G +E A+E G +N+ VY F L S++ K Sbjct: 677663 NYHQIPTVIFSHPPIGTIGYTEPEAVEKYGEENVKVYTSKFVNLLHSMADPERKGKT--A 677836 Query: 399 KLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIFTTL 457 +V + + VVG H G A E+ Q G+A+K+NA+ DF + V IHPT AE T+ Sbjct: 677837 MKLVTVGEKETVVGVHVAGEGADEMIQGFGVAVKMNATKADFDNIVAIHPTAAEELVTM 678013 Score = 65.9 bits (159), Expect = 1e-10 Identities = 93/371 (25%), Positives = 145/371 (39%), Gaps = 61/371 (16%) Frame = +1 Query: 145 VTFRRALIAVGGRP-KSLDCP-GADLAISSDDIF--SRPTPPGKTLVVGASYVALECAGF 200 +T + L+ VG RP + D P + SD + T P + ++VGA V +E A Sbjct: 170890 LTADKFLVCVGTRPARRPDIPFDGETIFDSDQLLWGKVKTVPRRLIIVGAGVVGMEYASM 171069 Query: 201 LKA-QGFDVTVMV-RSILLRGFDTDMAARIGTYMEEEAKIDFIKGAVPTSIEQLANGEG- 257 + G DVTV+ R +L D +++ + Y + F+ G S+E+ A GE Sbjct: 171070 MTIIPGTDVTVIDGRQEILNMADKEVSEAL-CYSMAQTGTRFLTGETIKSVEKTAKGEVF 171246 Query: 258 ---------IYDTVLNATGRNPDVSGLNLPAAGVGLNP-KTGRIAVTHE-QTSAPHIYA- 305 + D +L GR +V GLNL A VGL P K GRI V + QT+ PH+YA Sbjct: 171247 VHLESGKTVVGDGLLYTVGRQGNVEGLNLEA--VGLEPDKRGRIKVDNNFQTAVPHMYAR 171420 Query: 306 ---------------------VGDVIDGPELTPVAIQAGRLLA-----------KRLFDN 333 GD+I P L +++ GRL + K L D+ Sbjct: 171421 VIVHK*VQGLTAMFLCWDSYAAGDIIGFPGLASTSMEQGRLASVHMRTSKTLNTKELSDD 171600 Query: 334 SATLMAYDKVCT----------AVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLE 383 + D+V T ++T E G +E + +G Sbjct: 171601 K-KMDDPDRVRTRMRSGEVFPFGIYTVPEISMVGKNEQQLTKEQVPYEVGKARYEELARG 171777 Query: 384 WSLSHDRALAKECYCKLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVST 443 L K +C + +++G H +G A E+ + M + F + Sbjct: 171778 QMLGGVPGFLKIIFC------PETLKILGVHAIGEGATEIIHIGQVVMSTGGDLEYFRNA 171939 Query: 444 VGIHPTTAEIF 454 V +PT AE + Sbjct: 171940 VFNYPTLAEAY 171972 >PHYCAscaffold_51 Length = 529628 Score = 127 bits (320), Expect = 3e-29 Identities = 133/478 (27%), Positives = 209/478 (43%), Gaps = 24/478 (5%) Frame = +2 Query: 1 TYDWDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKK- 59 T D+D+VVIGGG GG A+ +AA+ G K ++ S LGGTC+NVGCIP K Sbjct: 380129 TNDYDVVVIGGGPGGYVAAIKAAQLGMKTACIE--------SRGKLGGTCLNVGCIPSKA 380284 Query: 60 LMHQSAIMGELLHKDAEAFGWAVPNATFDWTKLVTSVQDYIHGLNFKYRVDLRDKKVKYE 119 L+H + ++ H D +++G P ++ +++ + + + L + KV Y Sbjct: 380285 LLHSTHLLHTAQH-DFKSYGIDAPEVKANFPQMMKAKEKAVTTLTGGIESLFKKNKVTYI 380461 Query: 120 NFLGTFKDAHTLTLT-NPAKGKPPKDVTFRRALIAVGGRPKSLDCPGADLA----ISSDD 174 G +++ N KG + V + +IA G L D A I S Sbjct: 380462 KGFGKVSAQGEVSVALNDNKGN--ETVKAKNIIIATGSEVTPLPPVPVDNAAGKIIDSTG 380635 Query: 175 IFSRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVM-VRSILLRGFDTDMAARIGTYME 233 S P +VVGA + LE K G VTV+ G D A + T + Sbjct: 380636 ALSLTRVPEHLVVVGAGVIGLELGSVYKRLGSKVTVVEYLDAACPGMDKG-AVKEFTKLL 380812 Query: 234 EEAKIDFIKGAVPTSIEQLANGEGI----------------YDTVLNATGRNPDVSGLNL 277 ++ +DF T+ E NG+ + DTVL ATGR +GL L Sbjct: 380813 KKQGLDFKFSTKVTASE--VNGDVVKLTTEPSKGGDASSIECDTVLVATGRRAFTAGLGL 380986 Query: 278 PAAGVGLNPKTGRIAVTHE-QTSAPHIYAVGDVIDGPELTPVAIQAGRLLAKRLFDNSAT 336 G+ + K GRI V +T P I+A+GDVI G L A + G + + Sbjct: 380987 EQMGIQTD-KLGRIEVDDAFRTQVPGIFAIGDVIKGAMLAHKAEEEGIACVENIAGKHGH 381163 Query: 337 LMAYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHDRALAKEC 396 + Y + ++T E+ G +E E + A+ + F + S + A A + Sbjct: 381164 -VNYGAIPGVIYTFPEFASVGKTE----EELKAEGVEYNVGKFPMMANSRARTVAEA-DG 381325 Query: 397 YCKLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIF 454 K++ D+ + D+++G H + NAGE+ I ++ A+ +D T HPT +E F Sbjct: 381326 LVKVLADK-KTDKLLGVHIIASNAGEMISEGVIGIEYGAASEDLARTCHAHPTLSEAF 381496 >PHYCAscaffold_20 Length = 899172 Score = 106 bits (264), Expect = 9e-23 Identities = 73/207 (35%), Positives = 106/207 (51%), Gaps = 8/207 (3%) Frame = +1 Query: 259 YDTVLNATGRNPDVSGLNLPAAGVGLNPKTGRIAVT-HEQTSAPHIYAVGDVIDGPELTP 317 Y+ V+ A GR P + L L AGV + G I V ++QTS +++AVGD P LTP Sbjct: 776398 YEVVIYAAGRVPLTTSLQLDKAGVTTD-NHGFIQVDEYQQTSNSNVFAVGDACGTPALTP 776574 Query: 318 VAIQAGRLLAKRLFDNSATL-MAYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYH 376 VAI AGR L+ RLF ++Y+ + T VF+ G GL+E A G D + VY Sbjct: 776575 VAIAAGRRLSDRLFGGMKDAKISYENIPTVVFSHPPIGTIGLTEAQARCKYGDDEIKVYT 776754 Query: 377 QSFTPLEWSLSHD------RALAKECYCKLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIA 430 F + + L ++ K +V ++++VVG H +G A E+ Q G+A Sbjct: 776755 SRFVNMYYGLINEIDEETGEPKDKPKTAMKLVCAGKDEKVVGLHMIGMAADEILQGFGVA 776934 Query: 431 MKLNASYDDFVSTVGIHPTTAEIFTTL 457 +K+ A+ DF + IHPT E TL Sbjct: 776935 VKMGATKADFDDCIAIHPTAGEELVTL 777015 Score = 72.8 bits (177), Expect = 1e-12 Identities = 45/113 (39%), Positives = 60/113 (53%), Gaps = 9/113 (7%) Frame = +1 Query: 4 WDLVVIGGGSGGLAASKEAAKY-GQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLMH 62 +D +VIG GSGG+A+++ AA Y G +V V++ + LGGTCVNVGC+PKKLM Sbjct: 775417 YDYLVIGAGSGGMASARRAASYPGTRVAVVEQAR---------LGGTCVNVGCVPKKLMF 775569 Query: 63 QSAIMGELLHKDAEAFGW--------AVPNATFDWTKLVTSVQDYIHGLNFKY 107 +A M LH D +G+ FDW KL Y+ LN Y Sbjct: 775570 IAADMSHKLHHDFLHYGFEDEVTGGHLGKRTHFDWPKLKARRDAYVLRLNGIY 775728 Score = 54.7 bits (130), Expect = 3e-07 Identities = 36/98 (36%), Positives = 47/98 (47%), Gaps = 19/98 (19%) Frame = +2 Query: 143 KDVTFRRALIAVGGRPKSLDCPGA------------------DLAISSDDIFSRPTPPGK 184 K++ +IAVGG+P D PGA +L I SD F T P K Sbjct: 775892 KEILADNVMIAVGGKPLIPDIPGAYLFTTGKLGGTDFVLAGKELCIDSDGFFELETLPKK 776071 Query: 185 TLVVGASYVALECAGFLKAQGFDVTVMVRSI-LLRGFD 221 VVGA Y+A+E AG L A G + ++ R +LR FD Sbjct: 776072 VAVVGAGYIAVELAGVLNALGSETSIFCRKEGVLRTFD 776185 Database: P.capsici/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 64,023,748 Number of sequences in database: 917 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 35,799,854 Number of Sequences: 917 Number of extensions: 551599 Number of successful extensions: 2388 Number of sequences better than 1.0e-04: 4 Number of HSP's better than 0.0 without gapping: 66 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1822 Number of HSP's gapped (non-prelim): 1085 length of query: 474 length of database: 21,341,249 effective HSP length: 109 effective length of query: 365 effective length of database: 21,241,296 effective search space: 7753073040 effective search space used: 7753073040 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 108 (46.2 bits)