TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|288565202|gb|GG743995.1|:subseq(66495,30000) Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.113, whole genome shotgun sequence:[translate(1)] (474 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 276 5e-74 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 174 3e-43 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 147 2e-35 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 112 6e-35 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 60 1e-13 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 60 7e-09 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 276 bits (705), Expect = 5e-74 Identities = 170/356 (47%), Positives = 216/356 (60%), Gaps = 16/356 (4%) Frame = -2 Query: 114 KKVKYENFLGTFKDAHTLTLTNPAKGKPPKDVTFRRALIAVGGRPKSLDCPGAD--LAIS 171 KK+KY T+ + LT+ KG+ K +T + LI+VGGRP LD + L I+ Sbjct: 68104 KKIKY-----TYLK*INI*LTDK-KGEKTK-ITSKYILISVGGRPTFLDDIPNNR*LCIT 67946 Query: 172 SDDIFSRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVRSILLRGFDTDMAARIGTY 231 SDDIFS PPGKTL+VGASY+ALECAGFL +DVTVMVRSILLRGFD +MA +IG Y Sbjct: 67945 SDDIFSL*KPPGKTLIVGASYIALECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEY 67766 Query: 232 MEEEAKIDFIKGAVPTSIEQL----------ANG---EGIYDTVLNATGRNPDVSGLNLP 278 ME + FI+G +P+ I+Q ANG +YDT++ A GR+ D + L Sbjct: 67765 MERHG-VKFIRGTIPSDIKQEGEKKRVFWKDANG*TQSDVYDTIMLAIGRSSDT*NIGLE 67589 Query: 279 AAGVGLNPKTGRIAVTHEQTSAPHIYAVGDVID-GPELTPVAIQAGRLLAKRLFDNSATL 337 G+ P I + TS ++A+GD +D PELTP AI+AGRLL +RLF Sbjct: 67588 *LGIKTKPNGKIICNDDDTTSIEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKF 67409 Query: 338 MAYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHDRALAKECY 397 M Y + T VFTP+EYG G SE+ A G N+ V++ F PLEW+ S R K Y Sbjct: 67408 MNYHYIPTTVFTPLEYGTIGFSEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHDDKG-Y 67232 Query: 398 CKLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEI 453 CKLI++ N RVVGFHYLGPNAGEVT IA L A+ + F VGIHPT+AE+ Sbjct: 67231 CKLIINSADNKRVVGFHYLGPNAGEVT*GFAIAFILKATKEHFDDIVGIHPTSAEV 67064 Score = 138 bits (347), Expect = 2e-32 Identities = 73/136 (53%), Positives = 93/136 (68%), Gaps = 2/136 (1%) Frame = -1 Query: 2 YDWDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLM 61 Y +DL VIGGGSGGLAA+KEAA G KV + D+VKPSP GS WGLGGTCVNVGCIPKKLM Sbjct: 68525 YTYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKKLM 68346 Query: 62 HQSAIMGELLHKDAEAFGWA--VPNATFDWTKLVTSVQDYIHGLNFKYRVDLRDKKVKYE 119 H SA++GE H+ + GW+ + W +V V ++I LN+ Y+V L + +VKY Sbjct: 68345 HLSAVVGE-SHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKYF 68169 Query: 120 NFLGTFKDAHTLTLTN 135 N L T D +T+ + N Sbjct: 68168 NSLATLFDKNTVEVIN 68121 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 174 bits (440), Expect = 3e-43 Identities = 123/343 (35%), Positives = 173/343 (50%), Gaps = 62/343 (18%) Frame = +3 Query: 2 YDWDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQ--------------------G 41 YD+D+ +IGGGS GLA + EA K G K ++ DYV PS Q G Sbjct: 14232 YDYDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTYLYFIFLKRG 14411 Query: 42 STWGLGGTCVNVGCIPKKLMHQSAIMGELLHKDAEAFGWAVP--------NATFDWTKLV 93 + WG+GGTCVNVGCIPK+L H ++++ + + K A FG+ +F W +L+ Sbjct: 14412 T*WGIGGTCVNVGCIPKRLFHTASVIKDNILK-ANDFGFFDD*NFQNNP*AISFSWERLI 14588 Query: 94 TSVQDYIHGLNFKYRVDLRDKKVKYENFLGTFKDAHTLTLT-------------NPAKGK 140 +++ YI LN+K+ L K++ Y N L T KD +T+ T N + K Sbjct: 14589 SNI*SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIKNQNFEEIK 14768 Query: 141 PPKDVTFRRALIAVGGRPKSLDCPGADLAI----SSDDIFSRPTPPGKTLVVGASYVALE 196 K +T +I VGGRPKSL I +SDD+FS PGKTL+VG Y+A+E Sbjct: 14769 DNK-ITA*YIVICVGGRPKSLKINKK*EKI**MITSDDLFSL*KKPGKTLIVGGGYIAME 14945 Query: 197 CAGFLKAQGFDVTVMVRSILLRGFDTDMAARIGTYMEEEAKIDFIKGAVPTSIEQLANGE 256 C GFL G TV+ R+ L+ FD +M I M+E K++FI ++ SI ++ N E Sbjct: 14946 CGGFLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMKEVKKVNFIDKSII*SI*KIENNE 15125 Query: 257 -----------------GIYDTVLNATGRNPDVSGLNLPAAGV 282 +D +L A GR P+ LNL GV Sbjct: 15126 EKINVCYKNLNDNKITCD*FDLIL*AIGRKPNSKYLNLNNLGV 15254 Score = 71.6 bits (174), Expect(2) = 1e-14 Identities = 46/115 (40%), Positives = 63/115 (54%), Gaps = 1/115 (0%) Frame = +1 Query: 351 IEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHD-RALAKECYCKLIVDRTQNDR 409 IEY G+ E+ AI G DN+ VYH F PLE L+ + + K+I D++ ND+ Sbjct: 15817 IEYSFVGIHEE*AI*EYGIDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDK 15996 Query: 410 VVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIFTTLEITKESG 464 V+G HYLG NAGE+ +A N T+ IHPT+AE + I+K SG Sbjct: 15997 VLGIHYLGLNAGEIL*GYVVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSG 16161 Score = 27.3 bits (59), Expect(2) = 1e-14 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 9/50 (18%) Frame = +2 Query: 313 PELTPVAIQAGRLLAKRLFDNSATL---------MAYDKVCTAVFTPIEY 353 PELT A + RLLA R++ L M Y T +FTP+ + Sbjct: 15638 PELTSTA*MSSRLLAHRIYGYKNNLGED*MKNYKMNYKNTPTTLFTPLGF 15787 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 147 bits (372), Expect = 2e-35 Identities = 85/188 (45%), Positives = 116/188 (61%), Gaps = 17/188 (9%) Frame = -2 Query: 302 HIYAVGDVIDG-PELTPVAIQAGRLLAKRLF----DNSATLMAYDKVC-------TAVFT 349 +IYA+G+V+DG PELTPVA + G LLAKRL + + + K+ T +FT Sbjct: 58840 NIYAIGEVLDGIPELTPVA*KQG*LLAKRLIYKR*NKDINCVYF*KLKLDVNDFPTTIFT 58661 Query: 350 PIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLS--HDR---ALAKECYCKLIVDR 404 P EY C GLSE+ +I+ G +N+ VYH +TPLE +S +D +L ++ Y K+I ++ Sbjct: 58660 PTEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICNK 58481 Query: 405 TQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIFTTLEITKESG 464 +ND++VG HYLGPNAGEV +AMKL + D T+GIHPTT+E FT L ITK SG Sbjct: 58480 LENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSSG 58301 Query: 465 LDASAAGC 472 C Sbjct: 58300 EPYEKTSC 58277 Score = 91.3 bits (225), Expect = 2e-18 Identities = 62/153 (40%), Positives = 78/153 (50%), Gaps = 20/153 (13%) Frame = -3 Query: 87 FDWTKLVTSVQDYIHGLNFKYRVDLRDKKVKYENFLGTFKDAHTLT-------------- 132 F W LV +V YI +NF Y+ L DK + Y N L TF D +TL Sbjct: 59985 FIWENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*D 59806 Query: 133 --LTNPAKGKPPKDVTFRRALIAVGGRPKSLD---CPGA-DLAISSDDIFSRPTPPGKTL 186 L N K + +T +IAVGGRP L C + I+SDDIF PP KTL Sbjct: 59805 NNLIN*EK*EDLGKITAD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKTL 59626 Query: 187 VVGASYVALECAGFLKAQGFDVTVMVRSILLRG 219 VVG Y+A+ECAGFL G+ ++M R + LRG Sbjct: 59625 VVGGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 55.5 bits (132), Expect = 1e-07 Identities = 22/27 (81%), Positives = 25/27 (92%) Frame = -1 Query: 40 QGSTWGLGGTCVNVGCIPKKLMHQSAI 66 +G+TWGLGGTCVNVGCIPKKLMH S + Sbjct: 60197 RGNTWGLGGTCVNVGCIPKKLMHTSGL 60117 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 112 bits (281), Expect(2) = 6e-35 Identities = 78/197 (39%), Positives = 110/197 (55%), Gaps = 23/197 (11%) Frame = +3 Query: 48 GTCVNVGCIPKKLMHQSAIMGELLHKDAE-AFGWAVPNATFDWTKLVTSVQDYIHGLNFK 106 GT +NVG I KK MH +A GEL + +G + +W KL++ + +YI ++ Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 107 YRVDLRDKKVKYENFLGTFKDAHTLT--------LTNPAKGK-------------PPKDV 145 Y++DL + +VKY N + + + + L N K K + V Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 146 TFRRALIAVGGRPKSLDCPGADL-AISSDDIFSRPTPPGKTLVVGASYVALECAGFLKAQ 204 T + +IAVG RPK LD G+ I+SDD+F PP KTL+VGASYVALE AGFL + Sbjct: 107220 TAKFIIIAVGSRPKYLDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNSF 107399 Query: 205 GFDVTVMVRSILLRGFD 221 G++V+VMVRS +LRGFD Sbjct: 107400 GYNVSVMVRSKILRGFD 107450 Score = 85.9 bits (211), Expect(2) = 1e-22 Identities = 41/87 (47%), Positives = 58/87 (66%) Frame = +3 Query: 368 GADNLGVYHQSFTPLEWSLSHDRALAKECYCKLIVDRTQNDRVVGFHYLGPNAGEVTQAV 427 G +N+ V++ F PLEW+ S D + YCKLIV+ +N++VVG HYLGP+AGEV Sbjct: 108180 GEENVKVFYSIFRPLEWTFS-DTNEEDKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV*GY 108356 Query: 428 GIAMKLNASYDDFVSTVGIHPTTAEIF 454 +A LN + +DF GIHPT++E+F Sbjct: 108357 ALAFLLNGTKEDFDKAAGIHPTSSEVF 108437 Score = 54.7 bits (130), Expect(2) = 6e-35 Identities = 25/46 (54%), Positives = 32/46 (69%) Frame = +2 Query: 3 DWDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGG 48 D+D VIGGG GGL+ + EA +G K + D+V PSP G+ WGLGG Sbjct: 106616 DYDFFVIGGGCGGLSFA*EAC*FGAKGAIADFVYPSP*GNKWGLGG 106753 Score = 40.4 bits (93), Expect(2) = 1e-22 Identities = 19/47 (40%), Positives = 30/47 (63%) Frame = +1 Query: 321 QAGRLLAKRLFDNSATLMAYDKVCTAVFTPIEYGCCGLSEDAAIETI 367 + G LLA+RLF+ + LM Y + T +F+ +EY G SE+ AI+ + Sbjct: 108040 KTG*LLARRLFNGKSKLM*YINIPTIIFSSLEYATIGFSEEDAIDIL 108180 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 60.5 bits (145), Expect(2) = 1e-13 Identities = 62/232 (26%), Positives = 97/232 (41%), Gaps = 19/232 (8%) Frame = +2 Query: 148 RRALIAVGGRPKSLDCPGADL----AISSDDIFSRPTPPGKTLVVGASYVALECAGFLKA 203 + +IA G P PG + +SS S P P K +V+GA + LE Sbjct: 18044 KNIIIATGSEPTPF--PGLEFDEKTIVSSTGALSLPVIPEKLIVIGAGVIGLEMGSVY*R 18217 Query: 204 QGFDVTVMVRSILLRGFDTDMAARIGTYMEEEAKIDFIKGAVPT-----------SIEQL 252 G VTV+ + + F + A+ + ++ + G T ++E + Sbjct: 18218 LGTKVTVIEYAD*ICPFLDNEIAKAFHKTLTKYGLEILTGHKVTGG*NKGTNGIVNVEPV 18397 Query: 253 ANGEG---IYDTVLNATGRNPDVSGLNLPAAGVGLNPKTGRIAVTHEQ-TSAPHIYAVGD 308 GE + +L ATGR P V GLN G+ + K GRI + T+ P+IYA+GD Sbjct: 18398 KGGESKSLSANHILVATGRKPFVKGLNAQEIGIEFDNK-GRIVTNNLL*TNIPNIYAIGD 18574 Query: 309 VIDGPELTPVAIQAGRLLAKRLFDNSATLMAYDKVCTAVFTPIEYGCCGLSE 360 V+ GP L + G + A + YD + ++T E G +E Sbjct: 18575 VVAGPMLAHKGEEEG-IAAVENIAGKGGHVDYDAIPNVIYTHPEIAWVGKNE 18727 Score = 35.4 bits (80), Expect(2) = 1e-13 Identities = 21/68 (30%), Positives = 34/68 (50%), Gaps = 2/68 (2%) Frame = +3 Query: 387 SHDRALAKECYCKLIVDRTQ--NDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTV 444 ++ RA A + LI T+ D+++G H +G AGE+ + + AS +D T Sbjct: 18825 ANSRAKANNDFDGLIKVLTEINTDKILGVHIMGD*AGELISEAVLGISYGASAEDIGKTC 19004 Query: 445 GIHPTTAE 452 HPT +E Sbjct: 19005 HAHPTLSE 19028 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 59.7 bits (143), Expect = 7e-09 Identities = 78/284 (27%), Positives = 120/284 (42%), Gaps = 23/284 (8%) Frame = +1 Query: 100 IHGLNFKYRVDLRDKKVKYENFLGTFKDAHTL--TLTNPAKGKPPKDVTFRRALIAVGGR 157 + GL + KV Y G D +TL L+N K V+ + +IA G Sbjct: 28405 VKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*T----VSTKNIIIATGSE 28572 Query: 158 PKSLDCPGAD----LAISSDDIFSRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVR 213 P PG + +SS + P K +++GA + LE G VTV+ Sbjct: 28573 PSPF--PGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVTVVEF 28746 Query: 214 SILLRGF-DTDMAARIGTYMEEEAKIDFIKGAVPTS-----------IEQLANGEGIY-- 259 + + F D+D+A ++ I+ + G S IE + G I Sbjct: 28747 AD*ICPFLDSDIAKVFHKSLKHHG-IEILTGHKVISG*NLGTHAVVNIEPVKGGPSISL* 28923 Query: 260 -DTVLNATGRNPDVSGLNLPAAGVGLNPKTGRIAVTHEQ--TSAPHIYAVGDVIDGPELT 316 + VL ATGR P + GL GV ++ K GRI +T++ T+ P+IYA+GDV+ GP L Sbjct: 28924 AEHVLVATGRRPFLGGLG*DKVGVKMDDK-GRI-ITNDNL*TNIPNIYAIGDVVAGPMLA 29097 Query: 317 PVAIQAGRLLAKRLFDNSATLMAYDKVCTAVFTPIEYGCCGLSE 360 + G + A A + YD + ++T E G +E Sbjct: 29098 HKGEEEG-IAAVENIAGKAGHVNYDTIPNVIYTHPEIAWVGKNE 29226 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,369,402 Number of Sequences: 2017 Number of extensions: 165281 Number of successful extensions: 429 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 40 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 363 Number of HSP's gapped (non-prelim): 66 length of query: 474 length of database: 16,266,656 effective HSP length: 107 effective length of query: 367 effective length of database: 16,050,837 effective search space: 5890657179 effective search space used: 5890657179 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 107 (45.8 bits)