TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|288565202|gb|GG743995.1|:subseq(66495,30000) Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.113, whole genome shotgun sequence:[translate(1)] (474 letters) Database: G.niphandrodes/genome.fa 3680 sequences; 15,362,869 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|338242564|gb|AFNH01001883.1| Gregarina niphandrodes asmbl.266... 366 e-102 gi|338240660|gb|AFNH01002836.1| Gregarina niphandrodes asmbl.354... 145 4e-35 gi|338242696|gb|AFNH01001817.1| Gregarina niphandrodes asmbl.260... 117 7e-27 >gi|338242564|gb|AFNH01001883.1| Gregarina niphandrodes asmbl.2669, whole genome shotgun sequence Length = 19534 Score = 366 bits (940), Expect = e-102 Identities = 241/569 (42%), Positives = 305/569 (53%), Gaps = 99/569 (17%) Frame = +1 Query: 5 DLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLMHQS 64 DLVVIGGGSGGLAA+KEAA+ G KV+ DYV+PSPQGS WGLGGTCVNVGCIPK +MH S Sbjct: 12463 DLVVIGGGSGGLAAAKEAARLGAKVVCFDYVEPSPQGSKWGLGGTCVNVGCIPKIIMHNS 12642 Query: 65 AIMGELLHKDAEAFGWA-VPNATFDWTKLVTSVQDYIHGLNFKYRVDLRDKKVKYENFLG 123 A +G+L+ A GW+ TF W LV +VQ YI LNF YR LR V+Y N Sbjct: 12643 AKLGQLMDHSAAPLGWSGTDGLTFVWQDLVKTVQLYIKQLNFSYRRGLRSAGVEYINAKA 12822 Query: 124 TFKDAHTLTLTNPAKGK--------PPKDVTFRRALIAVGGRPK-SLDCPGA-DLAISSD 173 T A + G+ K+V + L+AVGGRP D PGA +LA++SD Sbjct: 12823 TLLAAPERAQSGAKSGRVVEYTLKGERKEVRAKCVLVAVGGRPYIPRDVPGAVELAVTSD 13002 Query: 174 DIFSRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVRSILLRGFDTDMAARIGTYME 233 D+FS P KTLVVGASY+ALECAGFL +DVTV VRSI+LRGFD A I M+ Sbjct: 13003 DLFSLSREPKKTLVVGASYIALECAGFLTHLRYDVTVCVRSIVLRGFDRQCAEHIQLVMK 13182 Query: 234 EE---------------------AKIDFI--KGAVPTS----------IEQLANG-EGIY 259 E+ A D +GA TS + + A+G E ++ Sbjct: 13183 EQGTKFLEHEQVVSLQRVGEPDVAASDLAGDRGADTTSNTTADGGDKILVRFASGREEMF 13362 Query: 260 DTVLNATGRNPDVSGLNLPAAGVGLNPKTGRIAVTH-EQTSAPHIYAVGDVIDG-PELTP 317 DTV+ A GR L AAG+ ++P +G+I V EQTSAP +YA+GDV G PELTP Sbjct: 13363 DTVVYAVGRRACTQSLGCEAAGIAMHPASGKIVVDELEQTSAPGVYAIGDVCHGRPELTP 13542 Query: 318 VAIQAGRLLAKRLFDNSATLMAYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQ 377 VAI+AG LLA+RLF + M Y + T VFTPIEYGCCGLSE+ AI G DN+ V+ Sbjct: 13543 VAIKAGELLARRLFGGANEPMVYTNIPTVVFTPIEYGCCGLSEEDAITRYGQDNIEVFLF 13722 Query: 378 SFTPLEWSLSH-DRALAKE----------CYCKLIVDRTQNDRVVGFHYLGPN------- 419 F + S H +AL+ I+ + ++++VG+H + N Sbjct: 13723 GFNSVPVSACHWKKALSTRKDEFDLDVTPLNLSKIICKKPDNKIVGYHCIAMNVSGHTSR 13902 Query: 420 ----------------------------------AGEVTQAVGIAMKLNASYDDFVSTVG 445 AGE TQ +A+K + DD + VG Sbjct: 13903 AQLGITPRVDWSGNWGRLARSDWLVWSGRKMHV*AGETTQGYALALKCGLTKDDLDAVVG 14082 Query: 446 IHPTTAEIFTTLEITKESGLDASAAGCUG 474 IHPT AE F ++ IT+ SG AAG G Sbjct: 14083 IHPTDAESFHSMTITRRSGESFMAAGGCG 14169 >gi|338240660|gb|AFNH01002836.1| Gregarina niphandrodes asmbl.3549, whole genome shotgun sequence Length = 8558 Score = 145 bits (365), Expect = 4e-35 Identities = 116/368 (31%), Positives = 172/368 (46%), Gaps = 50/368 (13%) Frame = +3 Query: 139 GKPPKDVTFRRALIAVGGRPKSLDCPG-ADLAISSDDIFSRPTPPGKTLVVGASYVALEC 197 G P + R ++AVG R K L PG A +A+ SD F+ P K VVGA Y+A+E Sbjct: 7002 GVPEVRLCAERVVVAVGSRSKKLGVPGEATVAVGSDGFFALERRPEKVAVVGAGYIAVEF 7181 Query: 198 AGFLKAQGFDVTVMVR-SILLRGFDTDMAARIGTYMEEEAKIDFIKGAVPTSIEQLANGE 256 AG L G + + +R +LR FD ++ + ++E + +GA ANG+ Sbjct: 7182 AGVLTGLGCETHLFLRHEKVLRSFDEMLSTKCTEFLERQG-CHLHRGAQVEEFIVDANGK 7358 Query: 257 GI----------YDTVLNATGRNPDVSGLNLPAAGVGLNPKTGRIAVTHEQT-------- 298 I +D+++ A GR P+ L L A V + G I V Q Sbjct: 7359 QIRLKDGRTFDHFDSIIVAIGREPNTDALGLDALNVRCD-SAGHIQVDDFQNVCFGQDNH 7535 Query: 299 ---------------------------SAPHIYAVGDVIDGPELTPVAIQAGRLLAKRLF 331 + +YA+GDVI ELTP+AI AGR LA RL+ Sbjct: 7536 AKDNHAKDDHAKDDHATNVSDVGPAVGADTGLYALGDVIGRVELTPMAIAAGRRLADRLY 7715 Query: 332 DNS--ATLMAYDKVCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHD 389 A +AY+ V T VF+ G GLSE A E G D++ VY + L +++ Sbjct: 7716 LEGFEAAKVAYEDVPTVVFSHPPIGTVGLSEAQARERFGNDDVLVYTNTAVNLYYNVFDV 7895 Query: 390 RALAK-ECYCKLIVDRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHP 448 K + Y KL+ + +++VVG H +G A E+ Q G+A+KL+A+ +D + V +HP Sbjct: 7896 IPTEKPKTYIKLVCVKHLHEQVVGIHVIGMGADEIIQGFGLALKLHATKNDLDNVVAVHP 8075 Query: 449 TTAEIFTT 456 + AE T Sbjct: 8076 SAAEEIVT 8099 Score = 62.4 bits (150), Expect = 3e-10 Identities = 33/76 (43%), Positives = 48/76 (63%) Frame = +1 Query: 4 WDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLMHQ 63 +DLVVIG GSGG+A +K A+ +G +V V++ GGTCVNVGC+PKK+M Sbjct: 6433 YDLVVIGAGSGGVACAKRASSHGARVAVVE---------AGAFGGTCVNVGCVPKKIMWW 6585 Query: 64 SAIMGELLHKDAEAFG 79 ++ E L ++A +G Sbjct: 6586 ASQAREFL-QEAHHYG 6630 >gi|338242696|gb|AFNH01001817.1| Gregarina niphandrodes asmbl.2608, whole genome shotgun sequence Length = 26051 Score = 117 bits (294), Expect = 7e-27 Identities = 138/487 (28%), Positives = 208/487 (42%), Gaps = 21/487 (4%) Frame = -3 Query: 4 WDLVVIGGGSGGLAASKEAAKYGQKVLVLDYVKPSPQGSTWGLGGTCVNVGCIPKKLMHQ 63 +DL V+G G GG A+ +AA+ G +V L+ + GGTC+NVGCIP K M Sbjct: 6081 FDLAVVGAGPGGYVAAIKAAQAGLRVACLERRALA--------GGTCLNVGCIPTKTMLH 5926 Query: 64 SAIMGELLHKDAEAFGWAVPNATFDWTKLVTS----VQDYIHGLNFKYRVDLRDKKVKYE 119 + E + A+ P + + KLV VQ G+ F + KK K E Sbjct: 5925 ITELAEQAGQQAKVLR-DKPGSGVELAKLVKHREQVVQQLGKGIGFLF------KKNKVE 5767 Query: 120 NFLGTFKDAHTLTLTNPAKGKPPKDVTFRRALIAVGGRPKSLDCPGADL----AISSDDI 175 G + A ++ T A G ++V + ++A G P L PG + +SSD Sbjct: 5766 ALWG--ESARFVSPTKLAFGN--REVEAKHVIVATGSTPAEL--PGLKIDEKHILSSDGA 5605 Query: 176 FSRPTPPGKTLVVGASYVALECAGFLKAQGFDVTVMVRSILLRG-FDTDMAARIGTYMEE 234 S P K LVVG V LE A + G V V+ + G DT +A + +E+ Sbjct: 5604 LSLTQAPKKLLVVGGGVVGLEFATVFRRLGSSVEVLEFLPQIGGALDTQVAQSLRKILEK 5425 Query: 235 E-AKID---FIKGAV----PTSIEQLANGEGIY---DTVLNATGRNPDVSGLNLPAAGVG 283 + KI ++GA ++ ANG + D VL GR + L L GV Sbjct: 5424 QGVKIHTGVAVQGATVEGDHVTVTAKANGTTMQFAGDKVLVCVGRRANTQNLGLEELGVE 5245 Query: 284 LNPKTGRIAV-THEQTSAPHIYAVGDVIDGPELTPVAIQAGRLLAKRLFDNSATLMAYDK 342 + GRI V H T+ I A+GD I GP L A + G + + + A+ Y Sbjct: 5244 -KDRAGRILVDEHYNTNVAPILAIGDCIPGPMLAHKAEEDGVAAVENILGHPASNHFYKY 5068 Query: 343 VCTAVFTPIEYGCCGLSEDAAIETIGADNLGVYHQSFTPLEWSLSHDRALAKECYCKLIV 402 V V+T E G +ED + A Y + P+ + + + K++V Sbjct: 5067 VPAVVYTSPECATVGATEDELKQARVA-----YKAASFPMAANSRSKCDGHTDGFVKVLV 4903 Query: 403 DRTQNDRVVGFHYLGPNAGEVTQAVGIAMKLNASYDDFVSTVGIHPTTAEIFTTLEITKE 462 D R++G + AGE+ + +A+K + +T+ HPT + E KE Sbjct: 4902 D-PATTRILGASIIAECAGEMITELTLAVKYGITATQLAATIHPHPTIS------EAVKE 4744 Query: 463 SGLDASA 469 + L ASA Sbjct: 4743 ACLAASA 4723 Database: G.niphandrodes/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 15,362,869 Number of sequences in database: 3680 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,634,066 Number of Sequences: 3680 Number of extensions: 161497 Number of successful extensions: 796 Number of sequences better than 1.0e-04: 3 Number of HSP's better than 0.0 without gapping: 451 Number of HSP's successfully gapped in prelim test: 37 Number of HSP's that attempted gapping in prelim test: 293 Number of HSP's gapped (non-prelim): 575 length of query: 474 length of database: 5,120,956 effective HSP length: 98 effective length of query: 376 effective length of database: 4,760,316 effective search space: 1789878816 effective search space used: 1789878816 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 103 (44.3 bits)