TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000081_1.0 # Protein # Thioredoxin reductase 3 (TR3) # Mus musculus # Complete (615 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 317 3e-86 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 173 2e-47 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 147 3e-35 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 115 2e-30 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 87 6e-17 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 79 2e-14 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 317 bits (812), Expect = 3e-86 Identities = 176/338 (52%), Positives = 227/338 (67%), Gaps = 2/338 (0%) Frame = -2 Query: 259 TNKKGQETFYTASKFVIATGERPRYLG-IQGDKEYCITSDDLFSLPYCPGCTLVVGASYV 317 T+KKG++T T+ +I+ G RP +L I ++ CITSDD+FSL PG TL+VGASY+ Sbjct: 68059 TDKKGEKTKITSKYILISVGGRPTFLDDIPNNR*LCITSDDIFSL*KPPGKTLIVGASYI 67880 Query: 318 GLECAGFLAGLGLDVTVMVRSVLLRGFDQEMAEKVGSYLEQQGVKFQRKFTPILVQQLEK 377 LECAGFLAG DVTVMVRS+LLRGFD EMA K+G Y+E+ GVKF R P ++Q Sbjct: 67879 ALECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEYMERHGVKFIRGTIPSDIKQ--- 67709 Query: 378 GLPGKLKVVAKSTEGPETVEGIYNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDV 437 G+ K V T +Y+T++LAIGR S T IGLE +G+K + NGKI ND Sbjct: 67708 --EGEKKRVFWKDANG*TQSDVYDTIMLAIGRSSDT*NIGLE*LGIK-TKPNGKIICNDD 67538 Query: 438 EQTNVPHVYAIGDILDGKPELTPVAIQAGKLLARRLFGVSLEKCDYINIPTTVFTPLEYG 497 + T++ V+AIGD +D +PELTP AI+AG+LL RRLFG + +Y IPTTVFTPLEYG Sbjct: 67537 DTTSIEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKFMNYHYIPTTVFTPLEYG 67358 Query: 498 CCGLSEEKAIEMYKKENLEVYHTLFWPLEWTVAG-RDNNTCYAKIICNKFDNERVVGFHL 556 G SEE A + N++V++++F PLEW + R ++ Y K+I N DN+RVVGFH Sbjct: 67357 TIGFSEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHDDKGYCKLIINSADNKRVVGFHY 67178 Query: 557 LGPNAGEITQGFAAAMKCGLTKQLLDDTIGIHPTCGEV 594 LGPNAGE+T GFA A TK+ DD +GIHPT EV Sbjct: 67177 LGPNAGEVT*GFAIAFILKATKEHFDDIVGIHPTSAEV 67064 Score = 127 bits (318), Expect = 5e-29 Identities = 65/154 (42%), Positives = 87/154 (56%), Gaps = 6/154 (3%) Frame = -1 Query: 127 HDYDXXXXXXXXXXXXCAKEAANLGKKVMVLDFVVPSPQGTTWGLGGTCVNVGCIPKKLM 186 + YD AKEAA+LG KV + DFV PSP G+ WGLGGTCVNVGCIPKKLM Sbjct: 68525 YTYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKKLM 68346 Query: 187 HQAALLGHALQDAKKYGW-EYNQQVKHNWEAMTEAIQSHIGSLNWGYRVTLREKGVTYVN 245 H +A++G + + K GW + + H WE M + +HI LNWGY+V L E V Y N Sbjct: 68345 HLSAVVGESHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKYFN 68166 Query: 246 SFGEFVDLHKIKATN-----KKGQETFYTASKFV 274 S D + ++ N KK + + +K++ Sbjct: 68165 SLATLFDKNTVEVINYFYIYKKNKIYIFKINKYI 68064 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 173 bits (438), Expect(2) = 2e-47 Identities = 98/188 (52%), Positives = 122/188 (64%), Gaps = 18/188 (9%) Frame = -2 Query: 444 HVYAIGDILDGKPELTPVAIQAGKLLARRLFGVSLEK---CDYI--------NIPTTVFT 492 ++YAIG++LDG PELTPVA + G LLA+RL K C Y + PTT+FT Sbjct: 58840 NIYAIGEVLDGIPELTPVA*KQG*LLAKRLIYKR*NKDINCVYF*KLKLDVNDFPTTIFT 58661 Query: 493 PLEYGCCGLSEEKAIEMYKKENLEVYHTLFWPLEWTVAGR---DNNT----CYAKIICNK 545 P EY C GLSEE +I+ Y +EN+EVYH+ + PLE ++ R D N+ YAKIICNK Sbjct: 58660 PTEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICNK 58481 Query: 546 FDNERVVGFHLLGPNAGEITQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSSG 605 +N+++VG H LGPNAGE+ G+A AMK G TK LD TIGIHPT E FT L ITKSSG Sbjct: 58480 LENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSSG 58301 Query: 606 LDITQKGC 613 + C Sbjct: 58300 EPYEKTSC 58277 Score = 77.8 bits (190), Expect = 3e-14 Identities = 53/151 (35%), Positives = 75/151 (49%), Gaps = 21/151 (13%) Frame = -3 Query: 214 WEAMTEAIQSHIGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKATNKKGQETFY----- 268 WE + + S+I S+N+GY+ L +K + YVN+ F D + + + K + Y Sbjct: 59979 WENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*DNN 59800 Query: 269 -------------TASKFVIATGERPRYLG---IQGDKEYCITSDDLFSLPYCPGCTLVV 312 TA VIA G RP L + +Y ITSDD+F P TLVV Sbjct: 59799 LIN*EK*EDLGKITAD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKTLVV 59620 Query: 313 GASYVGLECAGFLAGLGLDVTVMVRSVLLRG 343 G Y+ +ECAGFL+ LG ++M R + LRG Sbjct: 59619 GGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 54.7 bits (130), Expect = 3e-07 Identities = 22/27 (81%), Positives = 24/27 (88%) Frame = -1 Query: 165 QGTTWGLGGTCVNVGCIPKKLMHQAAL 191 +G TWGLGGTCVNVGCIPKKLMH + L Sbjct: 60197 RGNTWGLGGTCVNVGCIPKKLMHTSGL 60117 Score = 36.6 bits (83), Expect(2) = 2e-47 Identities = 16/33 (48%), Positives = 25/33 (75%) Frame = -3 Query: 400 YNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKI 432 +NT+L AIGR + T K+ L++IG+ IN +N K+ Sbjct: 59025 FNTILFAIGR*ANT*KLNLDRIGI*INNRNKKV 58927 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 147 bits (372), Expect = 3e-35 Identities = 111/351 (31%), Positives = 167/351 (47%), Gaps = 45/351 (12%) Frame = +3 Query: 118 QKLLQDDSAHDYDXXXXXXXXXXXXCAKEAANLGKKVMVLDFVVPSPQ------------ 165 Q+ + +++ +DYD A EA LG K ++ D+VVPS Q Sbjct: 14205 QENINNETKYDYDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTY 14384 Query: 166 --------GTTWGLGGTCVNVGCIPKKLMHQAALLGHALQDAKKYGWEYNQQVKHN---- 213 GT WG+GGTCVNVGCIPK+L H A+++ + A +G+ + ++N Sbjct: 14385 LYFIFLKRGT*WGIGGTCVNVGCIPKRLFHTASVIKDNILKANDFGFFDD*NFQNNP*AI 14564 Query: 214 ---WEAMTEAIQSHIGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKATNKKG------- 263 WE + I S+I LN+ + L K +TY+N+ D + I T K Sbjct: 14565 SFSWERLISNI*SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIK 14744 Query: 264 -------QETFYTASKFVIATGERPRYLGIQGDKEYC---ITSDDLFSLPYCPGCTLVVG 313 ++ TA VI G RP+ L I E ITSDDLFSL PG TL+VG Sbjct: 14745 NQNFEEIKDNKITA*YIVICVGGRPKSLKINKK*EKI**MITSDDLFSL*KKPGKTLIVG 14924 Query: 314 ASYVGLECAGFLAGLGLDVTVMVRSVLLRGFDQEMA-EKVGSYLEQQGVKFQRKFTPILV 372 Y+ +EC GFL+ LG+ TV+ R+ L+ FD+EM + + E + V F K + Sbjct: 14925 GGYIAMECGGFLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMKEVKKVNFIDKSII*SI 15104 Query: 373 QQLEKGLPGKLKVVAKSTEGPETVEGIYNTVLLAIGRDSCTRKIGLEKIGV 423 ++E K+ V K+ + ++ +L AIGR ++ + L +GV Sbjct: 15105 *KIENN-EEKINVCYKNLNDNKITCD*FDLIL*AIGRKPNSKYLNLNNLGV 15254 Score = 83.6 bits (205), Expect(3) = 1e-23 Identities = 52/123 (42%), Positives = 70/123 (56%), Gaps = 3/123 (2%) Frame = +1 Query: 494 LEYGCCGLSEEKAIEMYKKENLEVYHTLFWPLEWTVAGRDNN---TCYAKIICNKFDNER 550 +EY G+ EE AI Y +N+EVYH F PLE + N + KIIC+K +N++ Sbjct: 15817 IEYSFVGIHEE*AI*EYGIDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDK 15996 Query: 551 VVGFHLLGPNAGEITQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSSGLDITQ 610 V+G H LG NAGEI G+ A L K+ L+ TI IHPT E + I+KSSG + + Sbjct: 15997 VLGIHYLGLNAGEIL*GYVVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSGENFDK 16176 Query: 611 KGC 613 C Sbjct: 16177 DMC 16185 Score = 39.7 bits (91), Expect(3) = 1e-23 Identities = 22/55 (40%), Positives = 31/55 (56%), Gaps = 9/55 (16%) Frame = +2 Query: 451 ILDGKPELTPVAIQAGKLLARRLFG---------VSLEKCDYINIPTTVFTPLEY 496 I+ G PELT A + +LLA R++G + K +Y N PTT+FTPL + Sbjct: 15623 IIKGVPELTSTA*MSSRLLAHRIYGYKNNLGED*MKNYKMNYKNTPTTLFTPLGF 15787 Score = 26.6 bits (57), Expect(3) = 1e-23 Identities = 9/20 (45%), Positives = 17/20 (85%) Frame = +3 Query: 435 NDVEQTNVPHVYAIGDILDG 454 N+ E T++ +++A+GDIL+G Sbjct: 15519 NNYE*TSIQNIFAVGDILEG 15578 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 115 bits (288), Expect(2) = 2e-30 Identities = 74/197 (37%), Positives = 101/197 (51%), Gaps = 24/197 (12%) Frame = +3 Query: 173 GTCVNVGCIPKKLMHQAALLGHALQDAKKYGW-EYNQQVKHNWEAMTEAIQSHIGSLNWG 231 GT +NVG I KK MH AA G G+ + + + H W+ + I ++I + +W Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 232 YRVTLREKGVTYVNSFGEFVDLHKI-----------------------KATNKKGQETFY 268 Y++ L V Y N+F ++ +KI K K G Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 269 TASKFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGCTLVVGASYVGLECAGFLAGL 328 TA +IA G RP+YL ++G ++ ITSDDLF P TL+VGASYV LE AGFL Sbjct: 107220 TAKFIIIAVGSRPKYLDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNSF 107399 Query: 329 GLDVTVMVRSVLLRGFD 345 G +V+VMVRS +LRGFD Sbjct: 107400 GYNVSVMVRSKILRGFD 107450 Score = 78.2 bits (191), Expect = 3e-14 Identities = 38/87 (43%), Positives = 55/87 (63%), Gaps = 1/87 (1%) Frame = +3 Query: 510 YKKENLEVYHTLFWPLEWTVAG-RDNNTCYAKIICNKFDNERVVGFHLLGPNAGEITQGF 568 + +EN++V++++F PLEWT + + + Y K+I N N +VVG H LGP+AGE+ G+ Sbjct: 108177 FGEENVKVFYSIFRPLEWTFSDTNEEDKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV*GY 108356 Query: 569 AAAMKCGLTKQLLDDTIGIHPTCGEVF 595 A A TK+ D GIHPT EVF Sbjct: 108357 ALAFLLNGTKEDFDKAAGIHPTSSEVF 108437 Score = 49.3 bits (116), Expect = 1e-05 Identities = 25/55 (45%), Positives = 35/55 (63%) Frame = +1 Query: 464 QAGKLLARRLFGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYKKENLEVY 518 + G LLARRLF + YINIPT +F+ LEY G SEE AI++ +++ L + Sbjct: 108040 KTG*LLARRLFNGKSKLM*YINIPTIIFSSLEYATIGFSEEDAIDILERKMLRFF 108204 Score = 37.4 bits (85), Expect(2) = 2e-30 Identities = 20/46 (43%), Positives = 21/46 (45%) Frame = +2 Query: 128 DYDXXXXXXXXXXXXCAKEAANLGKKVMVLDFVVPSPQGTTWGLGG 173 DYD A EA G K + DFV PSP G WGLGG Sbjct: 106616 DYDFFVIGGGCGGLSFA*EAC*FGAKGAIADFVYPSP*GNKWGLGG 106753 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 87.0 bits (214), Expect = 6e-17 Identities = 78/290 (26%), Positives = 136/290 (46%), Gaps = 4/290 (1%) Frame = +2 Query: 223 SHIGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKA--TNKKGQETFYTASKFVIATGER 280 S + L G ++ V Y+ +G+ +D + I N G ++ +IATG Sbjct: 17897 SIVTGLTGGIEYLFKKNKVDYIKGYGKLLDKNTINVDLVNN*G**KL-SSKNIIIATGSE 18073 Query: 281 PR-YLGIQGDKEYCITSDDLFSLPYCPGCTLVVGASYVGLECAGFLAGLGLDVTVMVRSV 339 P + G++ D++ ++S SLP P +V+GA +GLE LG VTV+ + Sbjct: 18074 PTPFPGLEFDEKTIVSSTGALSLPVIPEKLIVIGAGVIGLEMGSVY*RLGTKVTVIEYAD 18253 Query: 340 LLRGF-DQEMAEKVGSYLEQQGVKFQRKFTPILVQQLEKGLPGKLKVVAKSTEGPETVEG 398 + F D E+A+ L + G++ + KG G + V + +G E+ Sbjct: 18254 *ICPFLDNEIAKAFHKTLTKYGLEILTGHK--VTGG*NKGTNGIVNV--EPVKGGESKSL 18421 Query: 399 IYNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDILDGKPEL 458 N +L+A GR + + ++IG++ + K G+I N++ TN+P++YAIGD++ G P L Sbjct: 18422 SANHILVATGRKPFVKGLNAQEIGIEFDNK-GRIVTNNLL*TNIPNIYAIGDVVAG-PML 18595 Query: 459 TPVAIQAGKLLARRLFGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIE 508 + G + G DY IP ++T E G +E + I+ Sbjct: 18596 AHKGEEEGIAAVENIAGKG-GHVDYDAIPNVIYTHPEIAWVGKNE*ELIK 18742 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 79.0 bits (193), Expect = 2e-14 Identities = 76/292 (26%), Positives = 131/292 (44%), Gaps = 3/292 (1%) Frame = +1 Query: 223 SHIGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKATNKKGQETFYTASKFVIATGERPR 282 S + L G ++ V Y+ FG+ D + + G + + +IATG P Sbjct: 28399 SIVKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*TVSTKNIIIATGSEPS 28578 Query: 283 -YLGIQGDKEYCITSDDLFSLPYCPGCTLVVGASYVGLECAGFLAGLGLDVTVMVRSVLL 341 + G+ D++ ++S +L P +++GA +GLE G VTV+ + + Sbjct: 28579 PFPGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVTVVEFAD*I 28758 Query: 342 RGF-DQEMAEKVGSYLEQQGVKFQRKFTPILVQQLEKGLPGKLKVV-AKSTEGPETVEGI 399 F D ++A+ L+ G++ I L G VV + +G ++ Sbjct: 28759 CPFLDSDIAKVFHKSLKHHGIEILTGHKVISG*NL-----GTHAVVNIEPVKGGPSISL* 28923 Query: 400 YNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDILDGKPELT 459 VL+A GR +G +K+GVK+++K G+I ND TN+P++YAIGD++ G P L Sbjct: 28924 AEHVLVATGRRPFLGGLG*DKVGVKMDDK-GRIITNDNL*TNIPNIYAIGDVVAG-PMLA 29097 Query: 460 PVAIQAGKLLARRLFGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYK 511 + G + G +Y IP ++T E G +E + I+ K Sbjct: 29098 HKGEEEGIAAVENIAG-KAGHVNYDTIPNVIYTHPEIAWVGKNE*ELIKAGK 29250 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,199,392 Number of Sequences: 2017 Number of extensions: 261812 Number of successful extensions: 579 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 36 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 505 Number of HSP's gapped (non-prelim): 68 length of query: 615 length of database: 16,266,656 effective HSP length: 109 effective length of query: 506 effective length of database: 16,046,803 effective search space: 8119682318 effective search space used: 8119682318 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 108 (46.2 bits)