TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000081_1.0 # Protein # Thioredoxin reductase 3 (TR3) # Mus musculus # Complete (615 letters) Database: G.niphandrodes/genome.fa 3680 sequences; 15,362,869 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|338242564|gb|AFNH01001883.1| Gregarina niphandrodes asmbl.266... 363 e-100 gi|338240660|gb|AFNH01002836.1| Gregarina niphandrodes asmbl.354... 162 3e-40 gi|338242696|gb|AFNH01001817.1| Gregarina niphandrodes asmbl.260... 132 3e-31 gi|338244915|gb|AFNH01000707.1| Gregarina niphandrodes asmbl.160... 50 2e-06 >gi|338242564|gb|AFNH01001883.1| Gregarina niphandrodes asmbl.2669, whole genome shotgun sequence Length = 19534 Score = 363 bits (931), Expect = e-100 Identities = 227/558 (40%), Positives = 306/558 (54%), Gaps = 86/558 (15%) Frame = +1 Query: 144 AKEAANLGKKVMVLDFVVPSPQGTTWGLGGTCVNVGCIPKKLMHQAALLGHALQ-DAKKY 202 AKEAA LG KV+ D+V PSPQG+ WGLGGTCVNVGCIPK +MH +A LG + A Sbjct: 12505 AKEAARLGAKVVCFDYVEPSPQGSKWGLGGTCVNVGCIPKIIMHNSAKLGQLMDHSAAPL 12684 Query: 203 GWEYNQQVKHNWEAMTEAIQSHIGSLNWGYRVTLREKGVTYVNSFGEFVDL-HKIKATNK 261 GW + W+ + + +Q +I LN+ YR LR GV Y+N+ + + ++ K Sbjct: 12685 GWSGTDGLTFVWQDLVKTVQLYIKQLNFSYRRGLRSAGVEYINAKATLLAAPERAQSGAK 12864 Query: 262 KGQETFYT---------ASKFVIATGERPRYL--GIQGDKEYCITSDDLFSLPYCPGCTL 310 G+ YT A ++A G RP Y+ + G E +TSDDLFSL P TL Sbjct: 12865 SGRVVEYTLKGERKEVRAKCVLVAVGGRP-YIPRDVPGAVELAVTSDDLFSLSREPKKTL 13041 Query: 311 VVGASYVGLECAGFLAGLGLDVTVMVRSVLLRGFDQEMAEKVGSYLEQQGVKF------- 363 VVGASY+ LECAGFL L DVTV VRS++LRGFD++ AE + +++QG KF Sbjct: 13042 VVGASYIALECAGFLTHLRYDVTVCVRSIVLRGFDRQCAEHIQLVMKEQGTKFLEHEQVV 13221 Query: 364 --QRKFTPILVQQLEKGLPGKLKVVAKSTEGPETV--------EGIYNTVLLAIGRDSCT 413 QR P + G G + +G + + E +++TV+ A+GR +CT Sbjct: 13222 SLQRVGEPDVAASDLAGDRGADTTSNTTADGGDKILVRFASGREEMFDTVVYAVGRRACT 13401 Query: 414 RKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDILDGKPELTPVAIQAGKLLARRL 473 + +G E G+ ++ +GKI V+++EQT+ P VYAIGD+ G+PELTPVAI+AG+LLARRL Sbjct: 13402 QSLGCEAAGIAMHPASGKIVVDELEQTSAPGVYAIGDVCHGRPELTPVAIKAGELLARRL 13581 Query: 474 FGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYKKENLEVYHTLFWPL-----EWT 528 FG + E Y NIPT VFTP+EYGCCGLSEE AI Y ++N+EV+ F + W Sbjct: 13582 FGGANEPMVYTNIPTVVFTPIEYGCCGLSEEDAITRYGQDNIEVFLFGFNSVPVSACHWK 13761 Query: 529 VAGRDNNTCY---------AKIICNKFDNERVVGFHLLGPN------------------- 560 A + +KIIC K DN ++VG+H + N Sbjct: 13762 KALSTRKDEFDLDVTPLNLSKIICKKPDN-KIVGYHCIAMNVSGHTSRAQLGITPRVDWS 13938 Query: 561 ----------------------AGEITQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTL 598 AGE TQG+A A+KCGLTK LD +GIHPT E F ++ Sbjct: 13939 GNWGRLARSDWLVWSGRKMHV*AGETTQGYALALKCGLTKDDLDAVVGIHPTDAESFHSM 14118 Query: 599 EITKSSGLD-ITQKGCUG 615 IT+ SG + GC G Sbjct: 14119 TITRRSGESFMAAGGCGG 14172 >gi|338240660|gb|AFNH01002836.1| Gregarina niphandrodes asmbl.3549, whole genome shotgun sequence Length = 8558 Score = 162 bits (410), Expect = 3e-40 Identities = 122/383 (31%), Positives = 188/383 (49%), Gaps = 40/383 (10%) Frame = +3 Query: 255 KIKATNKKGQETFYTASKFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGCTLVVGA 314 K KAT E A + V+A G R + LG+ G+ + SD F+L P VVGA Sbjct: 6981 KGKATTGGVPEVRLCAERVVVAVGSRSKKLGVPGEATVAVGSDGFFALERRPEKVAVVGA 7160 Query: 315 SYVGLECAGFLAGLGLDVTVMVR-SVLLRGFDQEMAEKVGSYLEQQGVKFQRKFTPILVQ 373 Y+ +E AG L GLG + + +R +LR FD+ ++ K +LE+QG R Sbjct: 7161 GYIAVEFAGVLTGLGCETHLFLRHEKVLRSFDEMLSTKCTEFLERQGCHLHRG------A 7322 Query: 374 QLEKGLPGKLKVVAKSTEGPETVEGIYNTVLLAIGRDSCTRKIGLEKIGVKINEKNGKIP 433 Q+E+ + + +G T + ++++++AIGR+ T +GL+ + V+ + G I Sbjct: 7323 QVEEFIVDANGKQIRLKDG-RTFDH-FDSIIVAIGREPNTDALGLDALNVRCDSA-GHIQ 7493 Query: 434 VNDVEQ------------------------TNVPHV----------YAIGDILDGKPELT 459 V+D + TNV V YA+GD++ G+ ELT Sbjct: 7494 VDDFQNVCFGQDNHAKDNHAKDDHAKDDHATNVSDVGPAVGADTGLYALGDVI-GRVELT 7670 Query: 460 PVAIQAGKLLARRLF--GVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYKKENLEV 517 P+AI AG+ LA RL+ G K Y ++PT VF+ G GLSE +A E + +++ V Sbjct: 7671 PMAIAAGRRLADRLYLEGFEAAKVAYEDVPTVVFSHPPIGTVGLSEAQARERFGNDDVLV 7850 Query: 518 YHTLFWPLEWTVAG---RDNNTCYAKIICNKFDNERVVGFHLLGPNAGEITQGFAAAMKC 574 Y L + V + Y K++C K +E+VVG H++G A EI QGF A+K Sbjct: 7851 YTNTAVNLYYNVFDVIPTEKPKTYIKLVCVKHLHEQVVGIHVIGMGADEIIQGFGLALKL 8030 Query: 575 GLTKQLLDDTIGIHPTCGEVFTT 597 TK LD+ + +HP+ E T Sbjct: 8031 HATKNDLDNVVAVHPSAAEEIVT 8099 Score = 50.4 bits (119), Expect = 2e-06 Identities = 28/75 (37%), Positives = 37/75 (49%) Frame = +1 Query: 129 YDXXXXXXXXXXXXCAKEAANLGKKVMVLDFVVPSPQGTTWGLGGTCVNVGCIPKKLMHQ 188 YD CAK A++ G +V V++ GGTCVNVGC+PKK+M Sbjct: 6433 YDLVVIGAGSGGVACAKRASSHGARVAVVE---------AGAFGGTCVNVGCVPKKIMWW 6585 Query: 189 AALLGHALQDAKKYG 203 A+ LQ+A YG Sbjct: 6586 ASQAREFLQEAHHYG 6630 >gi|338242696|gb|AFNH01001817.1| Gregarina niphandrodes asmbl.2608, whole genome shotgun sequence Length = 26051 Score = 132 bits (333), Expect = 3e-31 Identities = 127/437 (29%), Positives = 196/437 (44%), Gaps = 15/437 (3%) Frame = -3 Query: 172 GGTCVNVGCIPKKLM-------HQAALLGHALQDAKKYGWEYNQQVKHNWEAMTEAIQSH 224 GGTC+NVGCIP K M QA L+D G E + VKH E + + + Sbjct: 5976 GGTCLNVGCIPTKTMLHITELAEQAGQQAKVLRDKPGSGVELAKLVKHR-EQVVQQLGKG 5800 Query: 225 IGSLNWGYRVTLREKGVTYVNSFGEFVDLHKIKATNKKGQETFYTASKFVIATGERPRYL 284 IG L + K FV K+ N++ + A ++ATG P L Sbjct: 5799 IGFL------FKKNKVEALWGESARFVSPTKLAFGNREVE-----AKHVIVATGSTPAEL 5653 Query: 285 -GIQGDKEYCITSDDLFSLPYCPGCTLVVGASYVGLECAGFLAGLGLDVTVMVRSVLLRG 343 G++ D+++ ++SD SL P LVVG VGLE A LG V V+ + G Sbjct: 5652 PGLKIDEKHILSSDGALSLTQAPKKLLVVGGGVVGLEFATVFRRLGSSVEVLEFLPQIGG 5473 Query: 344 -FDQEMAEKVGSYLEQQGVKFQRKFTPILVQQLEKGLPGKLKVVAKSTEGPETVEGIYNT 402 D ++A+ + LE+QGVK T + VQ + G V G T++ + Sbjct: 5472 ALDTQVAQSLRKILEKQGVKIH---TGVAVQGAT--VEGDHVTVTAKANG-TTMQFAGDK 5311 Query: 403 VLLAIGRDSCTRKIGLEKIGVKINEKNGKIPVNDVEQTNVPHVYAIGDILDGKPELTPVA 462 VL+ +GR + T+ +GLE++GV+ ++ G+I V++ TNV + AIGD + G P L A Sbjct: 5310 VLVCVGRRANTQNLGLEELGVE-KDRAGRILVDEHYNTNVAPILAIGDCIPG-PMLAHKA 5137 Query: 463 IQAGKLLARRLFGVSLEKCDYINIPTTVFTPLEYGCCGLSEEKAIEMYKKENLEVYHTLF 522 + G + G Y +P V+T E G +E++ K+ Y Sbjct: 5136 EEDGVAAVENILGHPASNHFYKYVPAVVYTSPECATVGATEDEL-----KQARVAYKAAS 4972 Query: 523 WPLEWTVAGRDNNTC------YAKIICNKFDNERVVGFHLLGPNAGEITQGFAAAMKCGL 576 +P+ A + C + K++ + R++G ++ AGE+ A+K G+ Sbjct: 4971 FPM----AANSRSKCDGHTDGFVKVLVDP-ATTRILGASIIAECAGEMITELTLAVKYGI 4807 Query: 577 TKQLLDDTIGIHPTCGE 593 T L TI HPT E Sbjct: 4806 TATQLAATIHPHPTISE 4756 >gi|338244915|gb|AFNH01000707.1| Gregarina niphandrodes asmbl.16012, whole genome shotgun sequence Length = 77023 Score = 50.4 bits (119), Expect = 2e-06 Identities = 24/66 (36%), Positives = 38/66 (57%), Gaps = 2/66 (3%) Frame = +2 Query: 40 VMIFSKSYCPHSTRVKELFSSLGV--VYNILELDQVDDGASVQEVLTEISNQKTVPNIFV 97 V++FSK+YCP+ K+ + ELD + +G+S+Q L +S KTVP +F+ Sbjct: 76109 VVVFSKTYCPYCKIAKDALQKAVTDEYVKVYELDTMSEGSSIQAQLPALSGIKTVPQVFL 76288 Query: 98 NKVHVG 103 NK +G Sbjct: 76289 NKEFIG 76306 Database: G.niphandrodes/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 15,362,869 Number of sequences in database: 3680 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,414,643 Number of Sequences: 3680 Number of extensions: 166731 Number of successful extensions: 791 Number of sequences better than 1.0e-04: 4 Number of HSP's better than 0.0 without gapping: 282 Number of HSP's successfully gapped in prelim test: 38 Number of HSP's that attempted gapping in prelim test: 466 Number of HSP's gapped (non-prelim): 397 length of query: 615 length of database: 5,120,956 effective HSP length: 101 effective length of query: 514 effective length of database: 4,749,276 effective search space: 2441127864 effective search space used: 2441127864 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 104 (44.7 bits)