TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000080_1.0 # Protein # Thioredoxin reductase 2 (TR2) # Mus musculus # Complete (496 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 233 e-102 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 158 2e-38 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 104 1e-33 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 95 1e-23 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 57 6e-08 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 53 7e-07 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 233 bits (595), Expect(2) = e-102 Identities = 139/340 (40%), Positives = 199/340 (58%), Gaps = 32/340 (9%) Frame = -2 Query: 174 DKGGKATLLSAEHIVIATGGRPRYPTQVKGALEYGITSDDIFWLKESPGKTSVPAAEDVA 233 DK G+ T +++++I+I+ GGRP + + ITSDDIF L + PGKT + A +A Sbjct: 68056 DKKGEKTKITSKYILISVGGRPTFLDDIPNNR*LCITSDDIFSL*KPPGKTLIVGASYIA 67877 Query: 234 LECAGFLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTN 293 LECAGFL G D TVM+RSI LRGFD +M++ + E+ME HG +F++G +PS IK+ Sbjct: 67876 LECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEYMERHGVKFIRGTIPSDIKQ-EGE 67700 Query: 294 QLQVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQEATS 353 + +V W+D A+G + +DT++ AIGR +T + LE GI T P N KII + + TS Sbjct: 67699 KKRVFWKD-ANG*TQSDVYDTIMLAIGRSSDT*NIGLE*LGIKTKP-NGKIICNDDDTTS 67526 Query: 354 VPHIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKSSTLMDYS----------------- 396 + ++AIGD + RPELTPTAIKAG+LL +RLFG M+Y Sbjct: 67525 IEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKFMNYHYIPTTVFTPLEYGTIGF 67346 Query: 397 --------------NVYHAYYKPLEFTVAD-RDASQCYIKMVCMREPPQLVLGLHFLGPN 441 V+++ +KPLE+ +D R + Y K++ + V+G H+LGPN Sbjct: 67345 SEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHDDKGYCKLIINSADNKRVVGFHYLGPN 67166 Query: 442 AGEVTQGFALGIKCGASYAQVMQTVGIHPTCSEEVVKLHI 481 AGEVT GFA+ A+ VGIHPT S EV+ ++I Sbjct: 67165 AGEVT*GFAIAFILKATKEHFDDIVGIHPT-SAEVIYIYI 67049 Score = 158 bits (400), Expect(2) = e-102 Identities = 75/134 (55%), Positives = 98/134 (73%), Gaps = 1/134 (0%) Frame = -1 Query: 42 SFDLLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSPRGTKWGLGGTCVNVGCIPKKLMH 101 ++DL VIGGGSGGLA AKEAA LG KVA+AD+V+PSP G+KWGLGGTCVNVGCIPKKLMH Sbjct: 68522 TYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKKLMH 68343 Query: 102 QAALLGGMIRDAHHYGW-EVAQPVQHNWKTMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI 160 +A++G + GW ++ H W+ M V NH+K LNWG++V L + +VKYFN Sbjct: 68342 LSAVVGESHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKYFNS 68163 Query: 161 KASFVDEHTVRGVD 174 A+ D++TV ++ Sbjct: 68162 LATLFDKNTVEVIN 68121 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 158 bits (399), Expect = 2e-38 Identities = 114/343 (33%), Positives = 176/343 (51%), Gaps = 47/343 (13%) Frame = +3 Query: 43 FDLLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSP--------------------RGTK 82 +D+ +IGGGS GLA A EA +LG K + DYV PS RGT Sbjct: 14238 YDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTYLYFIFLKRGT* 14417 Query: 83 WGLGGTCVNVGCIPKKLMHQAALLGGMIRDAHHYGWEVAQPVQHN-------WKTMAEAV 135 WG+GGTCVNVGCIPK+L H A+++ I A+ +G+ Q+N W+ + + Sbjct: 14418 WGIGGTCVNVGCIPKRLFHTASVIKDNILKANDFGFFDD*NFQNNP*AISFSWERLISNI 14597 Query: 136 QNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGV-DKGG-------------KATL 181 +++ LN+ L +++ Y N A+ D++T+ DK K Sbjct: 14598 *SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIKNQNFEEIKDNK 14777 Query: 182 LSAEHIVIATGGRPRYPTQVKGALEY---GITSDDIFWLKESPGKTSVPAAEDVALECAG 238 ++A +IVI GGRP+ ++ E ITSDD+F L + PGKT + +A+EC G Sbjct: 14778 ITA*YIVICVGGRPK-SLKINKK*EKI**MITSDDLFSL*KKPGKTLIVGGGYIAMECGG 14954 Query: 239 FLTGIGLDTTVMMRSIPLRGFDQQMSSLVTEHM-ESHGTQFLKGCVPSHIKKLPTNQ--L 295 FL+ +G+ TTV+ R+ L+ FD++M + E+M E F+ + I K+ N+ + Sbjct: 14955 FLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMKEVKKVNFIDKSII*SI*KIENNEEKI 15134 Query: 296 QVTWEDHASGKEDTGTFDTVLWAIGRVPETRTLNLEKAGISTN 338 V +++ K FD +L AIGR P ++ LNL G+ T+ Sbjct: 15135 NVCYKNLNDNKITCD*FDLIL*AIGRKPNSKYLNLNNLGV*TS 15263 Score = 55.5 bits (132), Expect = 1e-07 Identities = 33/97 (34%), Positives = 53/97 (54%), Gaps = 3/97 (3%) Frame = +1 Query: 393 MDYSNVYHAYYKPLEFTVADRDAS---QCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGF 449 +D VYH + PLE + + + +IK++C + VLG+H+LG NAGE+ G+ Sbjct: 15871 IDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDKVLGIHYLGLNAGEIL*GY 16050 Query: 450 ALGIKCGASYAQVMQTVGIHPTCSEEVVKLHISKRSG 486 + + T+ IHPT +EE++ +ISK SG Sbjct: 16051 VVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSG 16161 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 104 bits (259), Expect(2) = 1e-33 Identities = 68/198 (34%), Positives = 106/198 (53%), Gaps = 24/198 (12%) Frame = +3 Query: 87 GTCVNVGCIPKKLMHQAALLGGMIRDAHHYGW-EVAQPVQHNWKTMAEAVQNHVKSLNWG 145 GT +NVG I KK MH AA G + + G+ +V H WK + + N++K+ +W Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 146 HRVQLQDRKVKYFNIKASFVDEHTV-----------------------RGVDKGGKATLL 182 +++ L + +VKY+N AS ++++ + + K G + Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 183 SAEHIVIATGGRPRYPTQVKGALEYGITSDDIFWLKESPGKTSVPAAEDVALECAGFLTG 242 +A+ I+IA G RP+Y V+G+ ++ ITSDD+F + P KT + A VALE AGFL Sbjct: 107220 TAKFIIIAVGSRPKY-LDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNS 107396 Query: 243 IGLDTTVMMRSIPLRGFD 260 G + +VM+RS LRGFD Sbjct: 107397 FGYNVSVMVRSKILRGFD 107450 Score = 58.9 bits (141), Expect(2) = 1e-33 Identities = 28/45 (62%), Positives = 32/45 (71%) Frame = +2 Query: 43 FDLLVIGGGSGGLACAKEAAQLGKKVAVADYVEPSPRGTKWGLGG 87 +D VIGGG GGL+ A EA G K A+AD+V PSP G KWGLGG Sbjct: 106619 YDFFVIGGGCGGLSFA*EAC*FGAKGAIADFVYPSP*GNKWGLGG 106753 Score = 58.9 bits (141), Expect = 1e-08 Identities = 29/78 (37%), Positives = 47/78 (60%), Gaps = 1/78 (1%) Frame = +3 Query: 398 VYHAYYKPLEFTVAD-RDASQCYIKMVCMREPPQLVLGLHFLGPNAGEVTQGFALGIKCG 456 V+++ ++PLE+T +D + + Y K++ + V+GLH+LGP+AGEV G+AL Sbjct: 108198 VFYSIFRPLEWTFSDTNEEDKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV*GYALAFLLN 108377 Query: 457 ASYAQVMQTVGIHPTCSE 474 + + GIHPT SE Sbjct: 108378 GTKEDFDKAAGIHPTSSE 108431 Score = 38.1 bits (87), Expect(3) = 3e-07 Identities = 27/102 (26%), Positives = 53/102 (51%), Gaps = 2/102 (1%) Frame = +3 Query: 251 MRSIPLRGF--DQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTNQLQVTWEDHASGKED 308 M+ IP+ + ++++S + H ++HG +F+K + I + +V ++ + Sbjct: 107616 MKLIPIINYK*QKEIASKIETHYKNHGIRFIKYSTINKIVL*IGSDKKVYYKK*E*SYLN 107795 Query: 309 TGTFDTVLWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQE 350 +FDT++ AIGR T+ LNL+K + N KII + + Sbjct: 107796 EESFDTIILAIGREA*TKRLNLDKINLKIK-NNGKIICNEDD 107918 Score = 28.5 bits (62), Expect(3) = 3e-07 Identities = 15/33 (45%), Positives = 19/33 (57%) Frame = +1 Query: 376 KAGKLLAQRLFGKSSTLMDYSNVYHAYYKPLEF 408 K G LLA+RLF S LM Y N+ + LE+ Sbjct: 108040 KTG*LLARRLFNGKSKLM*YINIPTIIFSSLEY 108138 Score = 25.8 bits (55), Expect(3) = 3e-07 Identities = 14/26 (53%), Positives = 16/26 (61%) Frame = +2 Query: 351 ATSVPHIYAIGDVAEGRPELTPTAIK 376 +TSV I+AIGD RP L TA K Sbjct: 107966 STSVDGIFAIGDCVYKRPMLGSTAKK 108043 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 94.7 bits (234), Expect(2) = 1e-23 Identities = 66/188 (35%), Positives = 92/188 (48%), Gaps = 49/188 (26%) Frame = -2 Query: 356 HIYAIGDVAEGRPELTPTAIKAGKLLAQRLFGKS------------------------ST 391 +IYAIG+V +G PELTP A K G LLA+RL K T Sbjct: 58840 NIYAIGEVLDGIPELTPVA*KQG*LLAKRLIYKR*NKDINCVYF*KLKLDVNDFPTTIFT 58661 Query: 392 LMDYS------------------NVYHAYYKPLEFTVA---DRDAS----QCYIKMVCMR 426 +YS VYH+ Y PLE ++ D D + + Y K++C + Sbjct: 58660 PTEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICNK 58481 Query: 427 EPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMQTVGIHPTCSEEVVKLHISKRSG 486 ++G+H+LGPNAGEV G+A+ +K G + + +T+GIHPT SEE L+I+K SG Sbjct: 58480 LENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSSG 58301 Query: 487 LEPTVTGC 494 T C Sbjct: 58300 EPYEKTSC 58277 Score = 81.6 bits (200), Expect = 2e-15 Identities = 57/154 (37%), Positives = 83/154 (53%), Gaps = 23/154 (14%) Frame = -3 Query: 128 WKTMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKG----------- 176 W+ + V +++KS+N+G++ L D+ + Y N A+F D++T+ K Sbjct: 59979 WENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*DNN 59800 Query: 177 ----------GKATLLSAEHIVIATGGRPRY-PTQ-VKGALEYGITSDDIFWLKESPGKT 224 GK T A+ IVIA GGRP P + K + +YGITSDDIF K P KT Sbjct: 59799 LIN*EK*EDLGKIT---AD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKT 59629 Query: 225 SVPAAEDVALECAGFLTGIGLDTTVMMRSIPLRG 258 V +A+ECAGFL+ +G T++M R + LRG Sbjct: 59628 LVVGGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 53.9 bits (128), Expect = 4e-07 Identities = 22/27 (81%), Positives = 23/27 (85%) Frame = -1 Query: 79 RGTKWGLGGTCVNVGCIPKKLMHQAAL 105 RG WGLGGTCVNVGCIPKKLMH + L Sbjct: 60197 RGNTWGLGGTCVNVGCIPKKLMHTSGL 60117 Score = 35.0 bits (79), Expect(2) = 1e-23 Identities = 16/34 (47%), Positives = 25/34 (73%) Frame = -3 Query: 312 FDTVLWAIGRVPETRTLNLEKAGISTNPKNQKII 345 F+T+L+AIGR T LNL++ GI N +N+K++ Sbjct: 59025 FNTILFAIGR*ANT*KLNLDRIGI*INNRNKKVL 58924 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 56.6 bits (135), Expect = 6e-08 Identities = 68/268 (25%), Positives = 117/268 (43%), Gaps = 3/268 (1%) Frame = +2 Query: 139 VKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKGGKAT--LLSAEHIVIATGGRPR 196 V L G + KV Y +D++T+ VD LS+++I+IATG P Sbjct: 17903 VTGLTGGIEYLFKKNKVDYIKGYGKLLDKNTIN-VDLVNN*G**KLSSKNIIIATGSEPT 18079 Query: 197 YPTQVKGALEYGITSDDIFWLKESPGKTSVPAAEDVALECAGFLTGIGLDTTVMMRSIPL 256 ++ + ++S L P K V A + LE +G TV+ + + Sbjct: 18080 PFPGLEFDEKTIVSSTGALSLPVIPEKLIVIGAGVIGLEMGSVY*RLGTKVTVIEYAD*I 18259 Query: 257 RGF-DQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTV 315 F D +++ + + +G + L G + TN + V E G+ + + + + Sbjct: 18260 CPFLDNEIAKAFHKTLTKYGLEILTGHKVTGG*NKGTNGI-VNVEPVKGGESKSLSANHI 18436 Query: 316 LWAIGRVPETRTLNLEKAGISTNPKNQKIIVDAQEATSVPHIYAIGDVAEGRPELTPTAI 375 L A GR P + LN ++ GI + K +I+ + T++P+IYAIGDV G P L Sbjct: 18437 LVATGRKPFVKGLNAQEIGIEFDNKG-RIVTNNLL*TNIPNIYAIGDVVAG-PMLAHKGE 18610 Query: 376 KAGKLLAQRLFGKSSTLMDYSNVYHAYY 403 + G + + GK +DY + + Y Sbjct: 18611 EEGIAAVENIAGKGGH-VDYDAIPNVIY 18691 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 53.1 bits (126), Expect = 7e-07 Identities = 67/266 (25%), Positives = 112/266 (42%), Gaps = 1/266 (0%) Frame = +1 Query: 139 VKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVRGVDKGGKATLLSAEHIVIATGGRPRYP 198 VK L G + KV Y D++T+ G +S ++I+IATG P Sbjct: 28405 VKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*TVSTKNIIIATGSEPSPF 28584 Query: 199 TQVKGALEYGITSDDIFWLKESPGKTSVPAAEDVALECAGFLTGIGLDTTVMMRSIPLRG 258 + + ++S L + P K + A + LE G TV+ + + Sbjct: 28585 PGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVTVVEFAD*ICP 28764 Query: 259 F-DQQMSSLVTEHMESHGTQFLKGCVPSHIKKLPTNQLQVTWEDHASGKEDTGTFDTVLW 317 F D ++ + + ++ HG + L G L T+ + V E G + + VL Sbjct: 28765 FLDSDIAKVFHKSLKHHGIEILTGHKVISG*NLGTHAV-VNIEPVKGGPSISL*AEHVLV 28941 Query: 318 AIGRVPETRTLNLEKAGISTNPKNQKIIVDAQEATSVPHIYAIGDVAEGRPELTPTAIKA 377 A GR P L +K G+ + K +II + T++P+IYAIGDV G P L + Sbjct: 28942 ATGRRPFLGGLG*DKVGVKMDDKG-RIITNDNL*TNIPNIYAIGDVVAG-PMLAHKGEEE 29115 Query: 378 GKLLAQRLFGKSSTLMDYSNVYHAYY 403 G + + GK+ ++Y + + Y Sbjct: 29116 GIAAVENIAGKAGH-VNYDTIPNVIY 29190 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.318 0.134 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,274,668 Number of Sequences: 2017 Number of extensions: 147501 Number of successful extensions: 365 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 34 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 303 Number of HSP's gapped (non-prelim): 64 length of query: 496 length of database: 16,266,656 effective HSP length: 107 effective length of query: 389 effective length of database: 16,050,837 effective search space: 6243775593 effective search space used: 6243775593 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 108 (46.2 bits)