TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000036_1.0 # Protein # Thioredoxin reductase 3 (TR3) # Homo sapiens # Complete (754 letters) Database: P.capsici/genome.fa 917 sequences; 64,023,748 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PHYCAscaffold_84 478 e-134 PHYCAscaffold_8 232 1e-60 PHYCAscaffold_20 122 2e-35 PHYCAscaffold_51 140 7e-33 PHYCAscaffold_16 84 8e-16 PHYCAscaffold_40 83 1e-15 PHYCAscaffold_18 82 2e-15 PHYCAscaffold_4 75 4e-13 PHYCAscaffold_71 49 4e-05 >PHYCAscaffold_84 Length = 182275 Score = 478 bits (1231), Expect = e-134 Identities = 258/504 (51%), Positives = 335/504 (66%), Gaps = 11/504 (2%) Frame = -3 Query: 262 EDLAYDYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIP 321 E+ YDYD C+KEAA G+KV VLD+V PSPQGTSWGLGGTCVNVGCIP Sbjct: 97748 EETNYDYDLVVIGGGSGGLACSKEAASFGQKVCVLDYVKPSPQGTSWGLGGTCVNVGCIP 97569 Query: 322 KKLMHQAALLGQALC-DSRKFGWEYNQ----QVRHNWETMTKAIQNHISSLNWGYRLSLR 376 KKLMHQ++L+G+ + D FGW + NW+ + + +I S+N+ Y++ LR Sbjct: 97568 KKLMHQSSLIGEVIHHDGANFGWNLSSGDGNAPTFNWKQLVSNVDGYIKSINFKYKVELR 97389 Query: 377 EKAVAYVNSYGEFVEHHKIKATNKKGQETYYTAAQFVIATGERPRYLGIQGDKEYCITSD 436 K V Y N G FV H ++ + + T VIA G RP+ L G E+ I+SD Sbjct: 97388 SKYVKYENFLGSFVNPHTLELWHPRKGTKQITTRDVVIAVGGRPKELTCPGG-EHAISSD 97212 Query: 437 DLFSLPYC-PGKTLVVGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYM 495 D+F + PGKTLVVGASYVALECAGFL G G DV VMVRSILLRGFDQ+MA+K+G YM Sbjct: 97211 DIFWMKKKEPGKTLVVGASYVALECAGFLKGMGYDVKVMVRSILLRGFDQDMADKIGEYM 97032 Query: 496 E-QHGVKFLRKFIPVMVQQLEKGSPGKLKVLAK-STEGTETIEGVYNTVLLAIGRDSCTR 553 E Q G++F+RK +P + +LE G ++L K ++E E+ E ++TVL A GRD Sbjct: 97031 EVQSGIEFIRKTVPQSITKLENG-----QLLVKWTSEDGESCEEAFDTVLNATGRDPDVA 96867 Query: 554 KIGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLF 613 K+ L+K GVK+NEKSG+I V + EQT+ P +YA+GD++ D PELTPVAIQ+G+LL++RL+ Sbjct: 96866 KLNLDKAGVKLNEKSGRIWVKN-EQTSTPNIYAIGDVI-DAPELTPVAIQAGRLLSRRLY 96693 Query: 614 GASLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGREN 673 S + DY V T VFTP+EYGCCG+SEE + E + ++N+E+YHT F PLEW+++ Sbjct: 96692 NNSTVQMDYEKVCTAVFTPIEYGCCGMSEEDSKERFGEDNIEVYHTNFVPLEWSLSTDTR 96513 Query: 674 ---NTCYAKIICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTC 730 +CY K+IC+K V+GFH LGPNAGEVTQ AMK G T + DT+GIHPT Sbjct: 96512 TAAESCYLKVICDKTRDKFVVGFHYLGPNAGEVTQAMGLAMKLGFTYDQMVDTVGIHPTT 96333 Query: 731 GEVFTTLEITKSSGLDITQKGCUG 754 E FTTLE+TKSSG T GC G Sbjct: 96332 AESFTTLEVTKSSGGATTGGGC*G 96261 >PHYCAscaffold_8 Length = 1194346 Score = 232 bits (592), Expect = 1e-60 Identities = 164/491 (33%), Positives = 244/491 (49%), Gaps = 8/491 (1%) Frame = +2 Query: 255 LLQKLLQEDLAYDYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTC 314 + ++ D+ YDY A+ AA G KV+V++ + GLGGTC Sbjct: 676595 IFPSIMSTDVVYDY--MVIGGGSGGMASARRAATYGAKVLVVE---RGREFDGMGLGGTC 676759 Query: 315 VNVGCIPKKLMHQAALLGQALCDSRKFGWEYNQQVRH------NWETMTKAIQNHISSLN 368 VN GC+PKK+M A + L +R + N + +W M +I LN Sbjct: 676760 VNFGCVPKKVMFNTAAHVEHL--NRNKDYSINTADKEFQFGDFDWNNMKIKRDAYIKRLN 676933 Query: 369 WGYRLSLREKAVAYVNSYGEFVEHHKIKATNKKGQETYYTAAQFVIATGERPRYLGIQGD 428 Y +L V +V + V KI+ +K YT ++A G P + G Sbjct: 676934 GIYERNLGNANVDHVQGIAKLVAKDKIQVGDK-----VYTGKHVLVAPGGVPSVPDLPG- 677095 Query: 429 KEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDVTVMVR-SILLRGFDQEM 487 E I SD F L P K VVGA Y+A+E AG D T+ R +LR FD + Sbjct: 677096 MENVIDSDGFFKLEQQPKKVAVVGAGYIAVEMAGIFNVLKSDTTLFCRFDQVLRKFDPIV 677275 Query: 488 AEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIG 547 + V ME+ GVKF+R + ++EK GKL + + G E ++T+L A+G Sbjct: 677276 RDLVNEEMEKAGVKFVRN---MRAARVEKQDNGKLTFVV-TINGEEQKFPDFDTILYAVG 677443 Query: 548 RDSCTRKIGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKL 607 R+ T+ +GLE++GVK++E G I V+ E T+VP VYA+GD ELTPVAI +G+ Sbjct: 677444 REPRTKDLGLEEVGVKLSE-GGFIEVDAQENTSVPGVYAIGDATVTGWELTPVAIAAGRR 677620 Query: 608 LAQRLFGASLEKC-DYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEW 666 L+ RLFG + C +Y +PT +F+ G G +E +A+E Y +EN+++Y + F L Sbjct: 677621 LSDRLFGGEKDACLNYHQIPTVIFSHPPIGTIGYTEPEAVEKYGEENVKVYTSKFVNLLH 677800 Query: 667 TVAGRENNTCYAKIICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGI 726 ++A E A + + + V+G H+ G A E+ QGF A+K TK D+ + I Sbjct: 677801 SMADPERKGKTAMKLVTVGEKETVVGVHVAGEGADEMIQGFGVAVKMNATKADFDNIVAI 677980 Query: 727 HPTCGEVFTTL 737 HPT E T+ Sbjct: 677981 HPTAAEELVTM 678013 >PHYCAscaffold_20 Length = 899172 Score = 122 bits (305), Expect(2) = 2e-35 Identities = 91/253 (35%), Positives = 130/253 (51%), Gaps = 10/253 (3%) Frame = +1 Query: 495 MEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRK 554 ME+ GV V V+Q + + VLA +E + Y V+ A GR T Sbjct: 776287 MEKDGVHLCPHSNVVRVEQAADNT--RTLVLADGSEHSG-----YEVVIYAAGRVPLTTS 776445 Query: 555 IGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFG 614 + L+K GV + G I V++ +QT+ V+AVGD P LTPVAI +G+ L+ RLFG Sbjct: 776446 LQLDKAGVT-TDNHGFIQVDEYQQTSNSNVFAVGDAC-GTPALTPVAIAAGRRLSDRLFG 776619 Query: 615 ASLE-KCDYINVPTTVFTPLEYGCCGLSEEKA--------IEVYKKENLEIYHTLFWPL- 664 + K Y N+PT VF+ G GL+E +A I+VY + +Y+ L + Sbjct: 776620 GMKDAKISYENIPTVVFSHPPIGTIGLTEAQARCKYGDDEIKVYTSRFVNMYYGLINEID 776799 Query: 665 EWTVAGRENNTCYAKIICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTI 724 E T ++ K++C D ++V+G H++G A E+ QGF A+K G TK DD I Sbjct: 776800 EETGEPKDKPKTAMKLVCAGKD-EKVVGLHMIGMAADEILQGFGVAVKMGATKADFDDCI 776976 Query: 725 GIHPTCGEVFTTL 737 IHPT GE TL Sbjct: 776977 AIHPTAGEELVTL 777015 Score = 52.4 bits (124), Expect = 3e-06 Identities = 37/130 (28%), Positives = 57/130 (43%), Gaps = 47/130 (36%) Frame = -2 Query: 179 VVIFSKSYCPHSTRVKELFS--------------------SLGVECNVLELDQVDD---G 215 V +FSKSYCP+ V+++ S GV+ +V+ELDQ +D G Sbjct: 623669 VTVFSKSYCPYCNLVRKVLSFGSLKELIRQVIR*AKGVLDEAGVKYHVVELDQKNDVPTG 623490 Query: 216 ARVQEVLSEITNQ------------------------KTVPNIFVNKVHVGGCDQTFQAY 251 A +Q L+ T + +TVPN+F+ K +GG + Sbjct: 623489 AEIQSALAAATGEHSSKWQQGNFNVVIDTSLVFALGRRTVPNVFIKKESIGGGTDVQALF 623310 Query: 252 QSGLLQKLLQ 261 QSG LQ++L+ Sbjct: 623309 QSGKLQEMLR 623280 Score = 48.1 bits (113), Expect(2) = 2e-35 Identities = 28/74 (37%), Positives = 38/74 (51%), Gaps = 1/74 (1%) Frame = +2 Query: 412 FVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDV 471 ++ TG+ + KE CI SD F L P K VVGA Y+A+E AG L G + Sbjct: 775964 YLFTTGKLGGTDFVLAGKELCIDSDGFFELETLPKKVAVVGAGYIAVELAGVLNALGSET 776143 Query: 472 TVMVRSI-LLRGFD 484 ++ R +LR FD Sbjct: 776144 SIFCRKEGVLRTFD 776185 >PHYCAscaffold_51 Length = 529628 Score = 140 bits (353), Expect = 7e-33 Identities = 129/475 (27%), Positives = 211/475 (44%), Gaps = 7/475 (1%) Frame = +2 Query: 267 DYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIPKK-LM 325 DYD A +AA LG K ++ + LGGTC+NVGCIP K L+ Sbjct: 380135 DYDVVVIGGGPGGYVAAIKAAQLGMKTACIE--------SRGKLGGTCLNVGCIPSKALL 380290 Query: 326 HQAALLGQALCDSRKFGWEYNQQVRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVNS 385 H LL A D + +G + +V+ N+ M KA + +++L G ++ V Y+ Sbjct: 380291 HSTHLLHTAQHDFKSYGIDA-PEVKANFPQMMKAKEKAVTTLTGGIESLFKKNKVTYIKG 380467 Query: 386 YGEFVEHHKIKAT--NKKGQETYYTAAQFVIATGERPRYLG---IQGDKEYCITSDDLFS 440 +G+ ++ + KG ET A +IATG L + I S S Sbjct: 380468 FGKVSAQGEVSVALNDNKGNETV-KAKNIIIATGSEVTPLPPVPVDNAAGKIIDSTGALS 380644 Query: 441 LPYCPGKTLVVGASYVALECAGFLAGFGLDVTVM-VRSILLRGFDQEMAEKVGSYMEQHG 499 L P +VVGA + LE G VTV+ G D+ ++ +++ G Sbjct: 380645 LTRVPEHLVVVGAGVIGLELGSVYKRLGSKVTVVEYLDAACPGMDKGAVKEFTKLLKKQG 380824 Query: 500 VKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRKIGLEK 559 + F KF + G KL +IE +TVL+A GR + T +GLE+ Sbjct: 380825 LDF--KFSTKVTASEVNGDVVKLTTEPSKGGDASSIE--CDTVLVATGRRAFTAGLGLEQ 380992 Query: 560 IGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFGASLEK 619 +G++ +K G+I V+D +T VP ++A+GD+++ L A + G + + G Sbjct: 380993 MGIQ-TDKLGRIEVDDAFRTQVPGIFAIGDVIKGAM-LAHKAEEEGIACVENIAGKH-GH 381163 Query: 620 CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGRENNTCYAK 679 +Y +P ++T E+ G +EE+ + N+ + + TVA + K Sbjct: 381164 VNYGAIPGVIYTFPEFASVGKTEEELKAEGVEYNVGKFPMMANSRARTVAEADG---LVK 381334 Query: 680 IICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGEVF 734 ++ +K D+++G HI+ NAGE+ ++ G + L T HPT E F Sbjct: 381335 VLADK-KTDKLLGVHIIASNAGEMISEGVIGIEYGAASEDLARTCHAHPTLSEAF 381496 >PHYCAscaffold_16 Length = 1020712 Score = 84.0 bits (206), Expect = 8e-16 Identities = 38/89 (42%), Positives = 60/89 (67%) Frame = +1 Query: 174 IERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPN 233 I S VV++SKSYCP+ TR K L + LG + +V+ELD+++ G+ Q+ L ++T Q TVPN Sbjct: 324109 ISASPVVVYSKSYCPYCTRTKTLLTELGAKFDVVELDEIEGGSEQQDALEDLTGQSTVPN 324288 Query: 234 IFVNKVHVGGCDQTFQAYQSGLLQKLLQE 262 +FV +GG + +++G L+ LL+E Sbjct: 324289 VFVGGKSIGGNSDVRKLHKAGNLEPLLKE 324375 >PHYCAscaffold_40 Length = 570383 Score = 83.2 bits (204), Expect = 1e-15 Identities = 38/93 (40%), Positives = 61/93 (65%) Frame = +1 Query: 174 IERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPN 233 I + VV++SKSYCP+ + K L + LG + +V+ELDQ+ G+ Q+ L EIT Q TVPN Sbjct: 467998 ISSTPVVVYSKSYCPYCAKTKTLLTQLGAKYDVVELDQISGGSEQQDALEEITGQSTVPN 468177 Query: 234 IFVNKVHVGGCDQTFQAYQSGLLQKLLQEDLAY 266 +FV +GG + +++G L+ LL+++ A+ Sbjct: 468178 VFVGGKSIGGNSDVQKLHKAGNLESLLEQNGAF 468276 >PHYCAscaffold_18 Length = 984210 Score = 82.4 bits (202), Expect = 2e-15 Identities = 36/89 (40%), Positives = 55/89 (61%) Frame = +1 Query: 174 IERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPN 233 I + +V++FSK++CP+ RVK L V+ELD DDG +Q +L +IT Q+TVPN Sbjct: 221992 IAKEKVLVFSKTHCPYCARVKGTLDVLDANFEVVELDTRDDGPEIQSLLLDITGQRTVPN 222171 Query: 234 IFVNKVHVGGCDQTFQAYQSGLLQKLLQE 262 +F+N H+GGCD + L +L++ Sbjct: 222172 VFINGKHIGGCDAVMDLHAKSELVPMLEK 222258 >PHYCAscaffold_4 Length = 1587695 Score = 75.1 bits (183), Expect = 4e-13 Identities = 39/94 (41%), Positives = 56/94 (59%) Frame = -1 Query: 174 IERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPN 233 I ++ +V+FSKSYCP+ + KE + LG + V ELD +DGA +Q L +T Q TVPN Sbjct: 325100 INQNPLVLFSKSYCPYCKKTKETLAGLGAKPVVFELDTREDGAAIQAFLFRLTRQSTVPN 324921 Query: 234 IFVNKVHVGGCDQTFQAYQSGLLQKLLQEDLAYD 267 +F+ VGG D + +SG L L++ A D Sbjct: 324920 LFIKGKSVGGNDNVQELQRSGELIDRLKKARAID 324819 >PHYCAscaffold_71 Length = 252329 Score = 48.5 bits (114), Expect = 4e-05 Identities = 53/220 (24%), Positives = 82/220 (37%), Gaps = 23/220 (10%) Frame = -3 Query: 268 YDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIPKKLMHQ 327 YD CA E+A GK+V ++D LGG CV+ G IP K + Sbjct: 40593 YDLVVIGSGPSATQCALESAKQGKRVAIVD--------KKTRLGGVCVHTGTIPSKTFRE 40438 Query: 328 AALLGQALCDSRKFGWEYNQQVRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYG 387 A L +G Y+ + E + +Q +SS R L+ V + + Sbjct: 40437 AVLHLSGYRHHGFYGKSYSMKT-VTIEDILYRVQRVVSSEEDVVRAQLKAARVDVIPGFA 40261 Query: 388 EFVEHHKIK--------------ATNKKGQE------TYYTAAQFVIATGERPRYLG-IQ 426 F H+I N G + A +F+IA G RP + + Sbjct: 40260 RFENQHEISIIRDDADVKKNGAVGANASGSSCNLDSLSRVHADKFLIACGTRPAHNPLVP 40081 Query: 427 GDKEYCITSDDLFS--LPYCPGKTLVVGASYVALECAGFL 464 D + I SD + S + P +V+GA + +E A + Sbjct: 40080 IDGKVIIDSDQILSRDMHQLPRSLIVMGAGVIGMEYASMV 39961 Database: P.capsici/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 64,023,748 Number of sequences in database: 917 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 57,441,009 Number of Sequences: 917 Number of extensions: 891477 Number of successful extensions: 4718 Number of sequences better than 1.0e-04: 9 Number of HSP's better than 0.0 without gapping: 64 Number of HSP's successfully gapped in prelim test: 45 Number of HSP's that attempted gapping in prelim test: 3707 Number of HSP's gapped (non-prelim): 2272 length of query: 754 length of database: 21,341,249 effective HSP length: 113 effective length of query: 641 effective length of database: 21,237,628 effective search space: 13613319548 effective search space used: 13613319548 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 110 (47.0 bits)