TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000036_1.0 # Protein # Thioredoxin reductase 3 (TR3) # Homo sapiens # Complete (754 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 325 e-119 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 166 1e-44 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 154 3e-37 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 125 2e-33 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 86 1e-16 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 82 2e-15 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 325 bits (832), Expect(2) = e-119 Identities = 177/338 (52%), Positives = 233/338 (68%), Gaps = 2/338 (0%) Frame = -2 Query: 398 TNKKGQETYYTAAQFVIATGERPRYLG-IQGDKEYCITSDDLFSLPYCPGKTLVVGASYV 456 T+KKG++T T+ +I+ G RP +L I ++ CITSDD+FSL PGKTL+VGASY+ Sbjct: 68059 TDKKGEKTKITSKYILISVGGRPTFLDDIPNNR*LCITSDDIFSL*KPPGKTLIVGASYI 67880 Query: 457 ALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEK 516 ALECAGFLAGF DVTVMVRSILLRGFD EMA K+G YME+HGVKF+R IP ++Q Sbjct: 67879 ALECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEYMERHGVKFIRGTIPSDIKQ--- 67709 Query: 517 GSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRKIGLEKIGVKINEKSGKIPVNDV 576 K +V K G T VY+T++LAIGR S T IGLE +G+K + +GKI ND Sbjct: 67708 -EGEKKRVFWKDANG-*TQSDVYDTIMLAIGRSSDT*NIGLE*LGIK-TKPNGKIICNDD 67538 Query: 577 EQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFGASLEKCDYINVPTTVFTPLEYG 636 + T++ V+A+GD ++ +PELTP AI++G+LL +RLFG + +Y +PTTVFTPLEYG Sbjct: 67537 DTTSIEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKFMNYHYIPTTVFTPLEYG 67358 Query: 637 CCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAG-RENNTCYAKIICNKFDHDRVIGFHI 695 G SEE A + N+++++++F PLEW + R ++ Y K+I N D+ RV+GFH Sbjct: 67357 TIGFSEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHDDKGYCKLIINSADNKRVVGFHY 67178 Query: 696 LGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGEV 733 LGPNAGEVT GFA A TK+ DD +GIHPT EV Sbjct: 67177 LGPNAGEVT*GFAIAFILKATKEHFDDIVGIHPTSAEV 67064 Score = 125 bits (313), Expect(2) = e-119 Identities = 61/135 (45%), Positives = 79/135 (58%), Gaps = 1/135 (0%) Frame = -1 Query: 266 YDYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNVGCIPKKLM 325 Y YD AKEAA LG KV + DFV PSP G+ WGLGGTCVNVGCIPKKLM Sbjct: 68525 YTYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKKLM 68346 Query: 326 HQAALLGQALCDSRKFGW-EYNQQVRHNWETMTKAIQNHISSLNWGYRLSLREKAVAYVN 384 H +A++G++ + GW + + H WE M + NHI LNWGY+++L E V Y N Sbjct: 68345 HLSAVVGESHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKYFN 68166 Query: 385 SYGEFVEHHKIKATN 399 S + + ++ N Sbjct: 68165 SLATLFDKNTVEVIN 68121 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 166 bits (420), Expect(2) = 1e-44 Identities = 92/187 (49%), Positives = 118/187 (63%), Gaps = 18/187 (9%) Frame = -2 Query: 584 VYAVGDILEDKPELTPVAIQSGKLLAQRLFGASLEK---CDYI--------NVPTTVFTP 632 +YA+G++L+ PELTPVA + G LLA+RL K C Y + PTT+FTP Sbjct: 58837 IYAIGEVLDGIPELTPVA*KQG*LLAKRLIYKR*NKDINCVYF*KLKLDVNDFPTTIFTP 58658 Query: 633 LEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGRENNT-------CYAKIICNKF 685 EY C GLSEE +I+ Y +EN+E+YH+ + PLE ++ R + YAKIICNK Sbjct: 58657 TEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICNKL 58478 Query: 686 DHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSSGL 745 ++D+++G H LGPNAGEV G+A AMK G TK LD TIGIHPT E FT L ITKSSG Sbjct: 58477 ENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSSGE 58298 Query: 746 DITQKGC 752 + C Sbjct: 58297 PYEKTSC 58277 Score = 78.2 bits (191), Expect = 3e-14 Identities = 52/151 (34%), Positives = 76/151 (50%), Gaps = 21/151 (13%) Frame = -3 Query: 353 WETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYGEFVEHHKIKATNKKGQETYY----- 407 WE + + ++I S+N+GY+ L +K + YVN+ F + + + + K + Y Sbjct: 59979 WENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*DNN 59800 Query: 408 -------------TAAQFVIATGERPRYLG---IQGDKEYCITSDDLFSLPYCPGKTLVV 451 TA VIA G RP L + +Y ITSDD+F P KTLVV Sbjct: 59799 LIN*EK*EDLGKITAD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKTLVV 59620 Query: 452 GASYVALECAGFLAGFGLDVTVMVRSILLRG 482 G Y+A+ECAGFL+ G ++M R + LRG Sbjct: 59619 GGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 53.1 bits (126), Expect = 1e-06 Identities = 21/27 (77%), Positives = 24/27 (88%) Frame = -1 Query: 304 QGTSWGLGGTCVNVGCIPKKLMHQAAL 330 +G +WGLGGTCVNVGCIPKKLMH + L Sbjct: 60197 RGNTWGLGGTCVNVGCIPKKLMHTSGL 60117 Score = 34.7 bits (78), Expect(2) = 1e-44 Identities = 15/33 (45%), Positives = 25/33 (75%) Frame = -3 Query: 539 YNTVLLAIGRDSCTRKIGLEKIGVKINEKSGKI 571 +NT+L AIGR + T K+ L++IG+ IN ++ K+ Sbjct: 59025 FNTILFAIGR*ANT*KLNLDRIGI*INNRNKKV 58927 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 154 bits (390), Expect = 3e-37 Identities = 113/351 (32%), Positives = 167/351 (47%), Gaps = 45/351 (12%) Frame = +3 Query: 257 QKLLQEDLAYDYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQ------------ 304 Q+ + + YDYD A EA LG K ++ D+VVPS Q Sbjct: 14205 QENINNETKYDYDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTY 14384 Query: 305 --------GTSWGLGGTCVNVGCIPKKLMHQAALLGQALCDSRKFGWEYNQQVRHN---- 352 GT WG+GGTCVNVGCIPK+L H A+++ + + FG+ + ++N Sbjct: 14385 LYFIFLKRGT*WGIGGTCVNVGCIPKRLFHTASVIKDNILKANDFGFFDD*NFQNNP*AI 14564 Query: 353 ---WETMTKAIQNHISSLNWGYRLSLREKAVAYVNSYGEFVEHHKIKATNKKG------- 402 WE + I ++IS LN+ + L K + Y+N+ + + I T K Sbjct: 14565 SFSWERLISNI*SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIK 14744 Query: 403 -------QETYYTAAQFVIATGERPRYLGIQGDKEYC---ITSDDLFSLPYCPGKTLVVG 452 ++ TA VI G RP+ L I E ITSDDLFSL PGKTL+VG Sbjct: 14745 NQNFEEIKDNKITA*YIVICVGGRPKSLKINKK*EKI**MITSDDLFSL*KKPGKTLIVG 14924 Query: 453 ASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYM-EQHGVKFLRKFIPVMV 511 Y+A+EC GFL+ G+ TV+ R+ L+ FD+EM + M E V F+ K I + Sbjct: 14925 GGYIAMECGGFLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMKEVKKVNFIDKSII*SI 15104 Query: 512 QQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRKIGLEKIGV 562 ++E K+ V K+ + ++ +L AIGR ++ + L +GV Sbjct: 15105 *KIENNEE-KINVCYKNLNDNKITCD*FDLIL*AIGRKPNSKYLNLNNLGV 15254 Score = 82.8 bits (203), Expect(3) = 1e-22 Identities = 50/123 (40%), Positives = 70/123 (56%), Gaps = 3/123 (2%) Frame = +1 Query: 633 LEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGRENN---TCYAKIICNKFDHDR 689 +EY G+ EE AI Y +N+E+YH F PLE + N + KIIC+K ++D+ Sbjct: 15817 IEYSFVGIHEE*AI*EYGIDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDK 15996 Query: 690 VIGFHILGPNAGEVTQGFAAAMKCGLTKQLLDDTIGIHPTCGEVFTTLEITKSSGLDITQ 749 V+G H LG NAGE+ G+ A L K+ L+ TI IHPT E + I+KSSG + + Sbjct: 15997 VLGIHYLGLNAGEIL*GYVVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSGENFDK 16176 Query: 750 KGC 752 C Sbjct: 16177 DMC 16185 Score = 38.9 bits (89), Expect(3) = 1e-22 Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 9/55 (16%) Frame = +2 Query: 590 ILEDKPELTPVAIQSGKLLAQRLFGASLE---------KCDYINVPTTVFTPLEY 635 I++ PELT A S +LLA R++G K +Y N PTT+FTPL + Sbjct: 15623 IIKGVPELTSTA*MSSRLLAHRIYGYKNNLGED*MKNYKMNYKNTPTTLFTPLGF 15787 Score = 24.6 bits (52), Expect(3) = 1e-22 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = +3 Query: 574 NDVEQTNVPYVYAVGDILE 592 N+ E T++ ++AVGDILE Sbjct: 15519 NNYE*TSIQNIFAVGDILE 15575 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 125 bits (313), Expect(2) = 2e-33 Identities = 78/197 (39%), Positives = 105/197 (53%), Gaps = 24/197 (12%) Frame = +3 Query: 312 GTCVNVGCIPKKLMHQAALLGQALCDSRKFGW-EYNQQVRHNWETMTKAIQNHISSLNWG 370 GT +NVG I KK MH AA G+ G+ + + + H W+ + I N+I + +W Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 371 YRLSLREKAVAYVNSYGEFVEHHKI-----------------------KATNKKGQETYY 407 Y++ L V Y N++ +E +KI K K G Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 408 TAAQFVIATGERPRYLGIQGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGF 467 TA +IA G RP+YL ++G ++ ITSDDLF P KTL+VGASYVALE AGFL F Sbjct: 107220 TAKFIIIAVGSRPKYLDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNSF 107399 Query: 468 GLDVTVMVRSILLRGFD 484 G +V+VMVRS +LRGFD Sbjct: 107400 GYNVSVMVRSKILRGFD 107450 Score = 95.1 bits (235), Expect = 3e-19 Identities = 78/266 (29%), Positives = 135/266 (50%), Gaps = 16/266 (6%) Frame = +3 Query: 485 QEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLL 544 +E+A K+ ++ + HG++F+ K+ + L GS K V K E + E ++T++L Sbjct: 107652 KEIASKIETHYKNHGIRFI-KYSTINKIVL*IGSDKK--VYYKK*E*SYLNEESFDTIIL 107822 Query: 545 AIGRDSCTRKIGLEKIGVKINEKSGKIPVN--DVEQTNVPYVYA------------VGDI 590 AIGR++ T+++ L+KI +KI + +GKI N DV ++Y + + Sbjct: 107823 AIGREA*TKRLNLDKINLKI-KNNGKIICNEDDVFYYISLFIYFYIFISLLVLMVYLQ*V 107999 Query: 591 LEDKPELTPVAIQSGKLL-AQRLFGASLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEVY 649 + +L V Q +L+ +++ F +E F + + Sbjct: 108000 IASIKDLC*VQQQKNRLIVSKKTF*WEIETYVIY*YSDYYF**FRICYNRIFRRGCY*YF 108179 Query: 650 KKENLEIYHTLFWPLEWTVAG-RENNTCYAKIICNKFDHDRVIGFHILGPNAGEVTQGFA 708 +EN+++++++F PLEWT + E + Y K+I N +++V+G H LGP+AGEV G+A Sbjct: 108180 GEENVKVFYSIFRPLEWTFSDTNEEDKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV*GYA 108359 Query: 709 AAMKCGLTKQLLDDTIGIHPTCGEVF 734 A TK+ D GIHPT EVF Sbjct: 108360 LAFLLNGTKEDFDKAAGIHPTSSEVF 108437 Score = 37.7 bits (86), Expect(2) = 2e-33 Identities = 20/46 (43%), Positives = 21/46 (45%) Frame = +2 Query: 267 DYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGG 312 DYD A EA G K + DFV PSP G WGLGG Sbjct: 106616 DYDFFVIGGGCGGLSFA*EAC*FGAKGAIADFVYPSP*GNKWGLGG 106753 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 86.3 bits (212), Expect = 1e-16 Identities = 73/288 (25%), Positives = 137/288 (47%), Gaps = 4/288 (1%) Frame = +2 Query: 364 ISSLNWGYRLSLREKAVAYVNSYGEFVEHHKIKA--TNKKGQETYYTAAQFVIATGERPR 421 ++ L G ++ V Y+ YG+ ++ + I N G ++ +IATG P Sbjct: 17903 VTGLTGGIEYLFKKNKVDYIKGYGKLLDKNTINVDLVNN*G**KL-SSKNIIIATGSEPT 18079 Query: 422 -YLGIQGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDVTVMVRSILL 480 + G++ D++ ++S SLP P K +V+GA + LE G VTV+ + + Sbjct: 18080 PFPGLEFDEKTIVSSTGALSLPVIPEKLIVIGAGVIGLEMGSVY*RLGTKVTVIEYAD*I 18259 Query: 481 RGF-DQEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVY 539 F D E+A+ + ++G++ L + KG+ G + V + +G E+ Sbjct: 18260 CPFLDNEIAKAFHKTLTKYGLEILTGH--KVTGG*NKGTNGIVNV--EPVKGGESKSLSA 18427 Query: 540 NTVLLAIGRDSCTRKIGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTP 599 N +L+A GR + + ++IG++ + K G+I N++ TN+P +YA+GD++ P L Sbjct: 18428 NHILVATGRKPFVKGLNAQEIGIEFDNK-GRIVTNNLL*TNIPNIYAIGDVVAG-PMLAH 18601 Query: 600 VAIQSGKLLAQRLFGASLEKCDYINVPTTVFTPLEYGCCGLSEEKAIE 647 + G + + G DY +P ++T E G +E + I+ Sbjct: 18602 KGEEEGIAAVENIAGKG-GHVDYDAIPNVIYTHPEIAWVGKNE*ELIK 18742 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 82.0 bits (201), Expect = 2e-15 Identities = 69/289 (23%), Positives = 134/289 (46%), Gaps = 2/289 (0%) Frame = +1 Query: 364 ISSLNWGYRLSLREKAVAYVNSYGEFVEHHKIKATNKKGQETYYTAAQFVIATGERPR-Y 422 + L G ++ V Y+ +G+ + + + G + + +IATG P + Sbjct: 28405 VKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*TVSTKNIIIATGSEPSPF 28584 Query: 423 LGIQGDKEYCITSDDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDVTVMVRSILLRG 482 G+ D++ ++S +L P K +++GA + LE FG VTV+ + + Sbjct: 28585 PGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVTVVEFAD*ICP 28764 Query: 483 F-DQEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNT 541 F D ++A+ ++ HG++ L ++ G+ + + + +G +I Sbjct: 28765 FLDSDIAKVFHKSLKHHGIEILTGH--KVISG*NLGTHAVVNI--EPVKGGPSISL*AEH 28932 Query: 542 VLLAIGRDSCTRKIGLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVA 601 VL+A GR +G +K+GVK+++K G+I ND TN+P +YA+GD++ P L Sbjct: 28933 VLVATGRRPFLGGLG*DKVGVKMDDK-GRIITNDNL*TNIPNIYAIGDVVAG-PMLAHKG 29106 Query: 602 IQSGKLLAQRLFGASLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEVYK 650 + G + + G + +Y +P ++T E G +E + I+ K Sbjct: 29107 EEEGIAAVENIAGKA-GHVNYDTIPNVIYTHPEIAWVGKNE*ELIKAGK 29250 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 26,534,745 Number of Sequences: 2017 Number of extensions: 310508 Number of successful extensions: 608 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 28 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 546 Number of HSP's gapped (non-prelim): 60 length of query: 754 length of database: 16,266,656 effective HSP length: 111 effective length of query: 643 effective length of database: 16,042,769 effective search space: 10315500467 effective search space used: 10315500467 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 109 (46.6 bits)