TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000034_1.0 # Protein # Thioredoxin reductase 1 (TR1) # Homo sapiens # Complete (499 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 325 7e-89 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 167 1e-45 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 138 2e-32 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 119 6e-30 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 83 3e-20 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 84 4e-16 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 325 bits (833), Expect = 7e-89 Identities = 177/338 (52%), Positives = 230/338 (68%), Gaps = 2/338 (0%) Frame = -2 Query: 143 TNNKGKEKIYSAERFLIATGERPRYLG-IPGDKEYCISSDDLFSLPYCPGKTLVVGASYV 201 T+ KG++ +++ LI+ G RP +L IP ++ CI+SDD+FSL PGKTL+VGASY+ Sbjct: 68059 TDKKGEKTKITSKYILISVGGRPTFLDDIPNNR*LCITSDDIFSL*KPPGKTLIVGASYI 67880 Query: 202 ALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEA 261 ALECAGFLAG DVTVMVRSILLRGFD +MANKIGE+ME HG+KFIR +P ++Q Sbjct: 67879 ALECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEYMERHGVKFIRGTIPSDIKQ--- 67709 Query: 262 GTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDE 321 G + V + Y+T+MLAIGR + T IGLE +G+K + GKI D+ Sbjct: 67708 --EGEKKRVFWKDANG*TQSDVYDTIMLAIGRSSDT*NIGLE*LGIK-TKPNGKIICNDD 67538 Query: 322 EQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYG 381 + T++ ++AIGD ++ + ELTP AI+AGRLL +RL+ G +Y +PTTVFTPLEYG Sbjct: 67537 DTTSIEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKFMNYHYIPTTVFTPLEYG 67358 Query: 382 ACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPS-RDNNKCYAKIICNTKDNERVVGFHV 440 G SEE A KFG+ NI+V++S F PLEW R ++K Y K+I N+ DN+RVVGFH Sbjct: 67357 TIGFSEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHDDKGYCKLIINSADNKRVVGFHY 67178 Query: 441 LGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEV 478 LGPNAGEVT GFA A TK+ D +GIHP AEV Sbjct: 67177 LGPNAGEVT*GFAIAFILKATKEHFDDIVGIHPTSAEV 67064 Score = 121 bits (303), Expect = 2e-27 Identities = 59/154 (38%), Positives = 87/154 (56%), Gaps = 6/154 (3%) Frame = -1 Query: 11 YDYDLIIIXXXXXXXXXXXXXXXXXXXVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLM 70 Y YDL +I V + DFV P+P+G++WGLGGTCVNVGCIPKKLM Sbjct: 68525 YTYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKKLM 68346 Query: 71 HQAALLGQALQDSRNYGWK-VEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYEN 129 H +A++G++ + +N GW +++ H W+ M+ V NHI LNWGY+VAL E +V Y N Sbjct: 68345 HLSAVVGESHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKYFN 68166 Query: 130 AYGQFIGPHRIKATN-----NKGKEKIYSAERFL 158 + + ++ N K K I+ +++ Sbjct: 68165 SLATLFDKNTVEVINYFYIYKKNKIYIFKINKYI 68064 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 167 bits (422), Expect(2) = 1e-45 Identities = 95/187 (50%), Positives = 118/187 (63%), Gaps = 18/187 (9%) Frame = -2 Query: 329 IYAIGDILEDKVELTPVAIQAGRLLAQRLYAGS-----------TVKCDYENVPTTVFTP 377 IYAIG++L+ ELTPVA + G LLA+RL +K D + PTT+FTP Sbjct: 58837 IYAIGEVLDGIPELTPVA*KQG*LLAKRLIYKR*NKDINCVYF*KLKLDVNDFPTTIFTP 58658 Query: 378 LEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSR---DNN----KCYAKIICNTK 430 EY GLSEE +++K+GEENIEVYHS + PLE I R D N K YAKIICN Sbjct: 58657 TEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICNKL 58478 Query: 431 DNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGA 490 +N+++VG H LGPNAGEV G+A A+K G TK LD TIGIHP +E FT L++TK SG Sbjct: 58477 ENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSSGE 58298 Query: 491 SILQAGC 497 + C Sbjct: 58297 PYEKTSC 58277 Score = 70.5 bits (171), Expect = 4e-12 Identities = 48/151 (31%), Positives = 74/151 (49%), Gaps = 21/151 (13%) Frame = -3 Query: 98 WDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIY----- 152 W+ ++ V ++I S+N+GY+ L +K + Y NA F + + + Y Sbjct: 59979 WENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*DNN 59800 Query: 153 -------------SAERFLIATGERPRYLG---IPGDKEYCISSDDLFSLPYCPGKTLVV 196 +A+ +IA G RP L +Y I+SDD+F P KTLVV Sbjct: 59799 LIN*EK*EDLGKITAD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKTLVV 59620 Query: 197 GASYVALECAGFLAGIGLDVTVMVRSILLRG 227 G Y+A+ECAGFL+ +G ++M R + LRG Sbjct: 59619 GGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 52.0 bits (123), Expect = 2e-06 Identities = 21/26 (80%), Positives = 22/26 (84%) Frame = -1 Query: 50 GTRWGLGGTCVNVGCIPKKLMHQAAL 75 G WGLGGTCVNVGCIPKKLMH + L Sbjct: 60194 GNTWGLGGTCVNVGCIPKKLMHTSGL 60117 Score = 36.2 bits (82), Expect(2) = 1e-45 Identities = 29/106 (27%), Positives = 58/106 (54%), Gaps = 17/106 (16%) Frame = -3 Query: 228 FDQDMANKIGEHM-EEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQST--NSEE------ 278 FD D+A +I ++ +E+G+ + +P +E+++ ++++V T +E+ Sbjct: 59238 FD*DIAYRIVYNL*KENGVNIVPTSLPSSIEKVDENL-FKVKIVN*VT**ETEDCIFLYI 59062 Query: 279 --------IIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKI 316 II+G +NT++ AIGR A T K+ L+ +G+ IN + K+ Sbjct: 59061 YYLIHFIIIIKG-FNTILFAIGR*ANT*KLNLDRIGI*INNRNKKV 58927 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 138 bits (347), Expect = 2e-32 Identities = 100/342 (29%), Positives = 166/342 (48%), Gaps = 45/342 (13%) Frame = +3 Query: 11 YDYDLIIIXXXXXXXXXXXXXXXXXXXVMVLDFVTPTPL--------------------G 50 YDYD+ II ++ D+V P+ G Sbjct: 14232 YDYDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTYLYFIFLKRG 14411 Query: 51 TRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHD-------WDRMIE 103 T WG+GGTCVNVGCIPK+L H A+++ + + ++G+ + +++ W+R+I Sbjct: 14412 T*WGIGGTCVNVGCIPKRLFHTASVIKDNILKANDFGFFDD*NFQNNP*AISFSWERLIS 14591 Query: 104 AVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKG--------------KE 149 + ++I LN+ + L K++ Y NA + I T +K K+ Sbjct: 14592 NI*SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIKNQNFEEIKD 14771 Query: 150 KIYSAERFLIATGERPRYLGIPGDKEYC---ISSDDLFSLPYCPGKTLVVGASYVALECA 206 +A +I G RP+ L I E I+SDDLFSL PGKTL+VG Y+A+EC Sbjct: 14772 NKITA*YIVICVGGRPKSLKINKK*EKI**MITSDDLFSL*KKPGKTLIVGGGYIAMECG 14951 Query: 207 GFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEE-HGIKFIRQFVPIKVEQIEAGTPG 265 GFL+ +G+ TV+ R+ L+ FD++M I E+M+E + FI + + + +IE Sbjct: 14952 GFLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMKEVKKVNFIDKSII*SI*KIE-NNEE 15128 Query: 266 RLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGV 307 ++ V ++ N +I ++ ++ AIGR ++ + L +GV Sbjct: 15129 KINVCYKNLNDNKITCD*FDLIL*AIGRKPNSKYLNLNNLGV 15254 Score = 86.3 bits (212), Expect(2) = 9e-22 Identities = 50/123 (40%), Positives = 72/123 (58%), Gaps = 3/123 (2%) Frame = +1 Query: 378 LEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNN---KCYAKIICNTKDNER 434 +EY G+ EE A+ ++G +NIEVYH F PLE + N K + KIIC+ +N++ Sbjct: 15817 IEYSFVGIHEE*AI*EYGIDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDK 15996 Query: 435 VVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQ 494 V+G H LG NAGE+ G+ A L KK L+ TI IHP AE + +++K SG + + Sbjct: 15997 VLGIHYLGLNAGEIL*GYVVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSGENFDK 16176 Query: 495 AGC 497 C Sbjct: 16177 DMC 16185 Score = 37.0 bits (84), Expect(2) = 9e-22 Identities = 25/68 (36%), Positives = 33/68 (48%), Gaps = 15/68 (22%) Frame = +2 Query: 328 YIYAIGDI------LEDKVELTPVAIQAGRLLAQRLYAGST---------VKCDYENVPT 372 YIY I I ++ ELT A + RLLA R+Y K +Y+N PT Sbjct: 15584 YIYIINIIFFFIFIIKGVPELTSTA*MSSRLLAHRIYGYKNNLGED*MKNYKMNYKNTPT 15763 Query: 373 TVFTPLEY 380 T+FTPL + Sbjct: 15764 TLFTPLGF 15787 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 119 bits (299), Expect(2) = 6e-30 Identities = 76/197 (38%), Positives = 106/197 (53%), Gaps = 24/197 (12%) Frame = +3 Query: 57 GTCVNVGCIPKKLMHQAALLGQALQDSRNYGW-KVEETVKHDWDRMIEAVQNHIGSLNWG 115 GT +NVG I KK MH AA G+ N G+ V+ H+W ++I + N+I + +W Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 116 YRVALREKKVVYENAYGQFIGPHRI-----------------------KATNNKGKEKIY 152 Y++ L +V Y NA+ I ++I K G + Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 153 SAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGI 212 +A+ +IA G RP+YL + G ++ I+SDDLF P KTL+VGASYVALE AGFL Sbjct: 107220 TAKFIIIAVGSRPKYLDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNSF 107399 Query: 213 GLDVTVMVRSILLRGFD 229 G +V+VMVRS +LRGFD Sbjct: 107400 GYNVSVMVRSKILRGFD 107450 Score = 107 bits (268), Expect = 2e-23 Identities = 81/270 (30%), Positives = 133/270 (49%), Gaps = 20/270 (7%) Frame = +3 Query: 230 QDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVML 289 +++A+KI H + HGI+FI+ + +I +V + + E ++T++L Sbjct: 107652 KEIASKIETHYKNHGIRFIKYST---INKIVL*IGSDKKVYYKK*E*SYLNEESFDTIIL 107822 Query: 290 AIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQ----TNVPYIYAIGDILEDKV----- 340 AIGR+A T+++ L+ + +KI + GKI +++ + Y Y +L V Sbjct: 107823 AIGREA*TKRLNLDKINLKI-KNNGKIICNEDDVFYYISLFIYFYIFISLLVLMVYLQ*V 107999 Query: 341 -----ELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEK-- 393 +L V Q RL+ + T + E ++ + + + + Sbjct: 108000 IASIKDLC*VQQQKNRLIVSK----KTF*WEIETYVIY*YSDYYF**FRICYNRIFRRGC 108167 Query: 394 ---FGEENIEVYHSYFWPLEWTIP-SRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVT 449 FGEEN++V++S F PLEWT + + +K Y K+I N K N +VVG H LGP+AGEV Sbjct: 108168 Y*YFGEENVKVFYSIFRPLEWTFSDTNEEDKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV 108347 Query: 450 QGFAAALKCGLTKKQLDSTIGIHPVCAEVF 479 G+A A TK+ D GIHP +EVF Sbjct: 108348 *GYALAFLLNGTKEDFDKAAGIHPTSSEVF 108437 Score = 30.8 bits (68), Expect(2) = 6e-30 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +2 Query: 40 VLDFVTPTPLGTRWGLGG 57 + DFV P+P G +WGLGG Sbjct: 106700 IADFVYPSP*GNKWGLGG 106753 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 83.2 bits (204), Expect(2) = 3e-20 Identities = 75/287 (26%), Positives = 131/287 (45%), Gaps = 8/287 (2%) Frame = +2 Query: 109 IGSLNWGYRVALREKKVVYENAYGQFIGPHRIKA--TNNKGKEKIYSAERFLIATGERPR 166 + L G ++ KV Y YG+ + + I NN G K+ S++ +IATG P Sbjct: 17903 VTGLTGGIEYLFKKNKVDYIKGYGKLLDKNTINVDLVNN*G**KL-SSKNIIIATGSEPT 18079 Query: 167 -YLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILL 225 + G+ D++ +SS SLP P K +V+GA + LE +G VTV+ + + Sbjct: 18080 PFPGLEFDEKTIVSSTGALSLPVIPEKLIVIGAGVIGLEMGSVY*RLGTKVTVIEYAD*I 18259 Query: 226 RGF-DQDMANKIGEHMEEHGIKFIRQFVPIKVE-QIEAGTPGRLRVVAQSTNSEEIIEGE 283 F D ++A + + ++G++ + KV GT G + V + + Sbjct: 18260 CPFLDNEIAKAFHKTLTKYGLEILTGH---KVTGG*NKGTNGIVNVEPVKGGESKSLSA- 18427 Query: 284 YNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELT 343 N +++A GR + + + +G++ + K G+I + TN+P IYAIGD++ Sbjct: 18428 -NHILVATGRKPFVKGLNAQEIGIEFDNK-GRIVTNNLL*TNIPNIYAIGDVVAG----- 18586 Query: 344 PVAIQAGR---LLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSE 387 P+ G + A AG DY+ +P ++T E G +E Sbjct: 18587 PMLAHKGEEEGIAAVENIAGKGGHVDYDAIPNVIYTHPEIAWVGKNE 18727 Score = 34.7 bits (78), Expect(2) = 3e-20 Identities = 20/53 (37%), Positives = 32/53 (60%), Gaps = 1/53 (1%) Frame = +3 Query: 52 RWGLGGTCVNVGCIPKK-LMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIE 103 R LGGTC+NVGCIP K L++ + ++ ++G + + VK +W R I+ Sbjct: 17676 RGALGGTCLNVGCIPSKALLNIS*KYFDSIHKFADFGIEA-KGVKVNWGRKIK 17831 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 84.0 bits (206), Expect = 4e-16 Identities = 75/294 (25%), Positives = 132/294 (44%), Gaps = 5/294 (1%) Frame = +1 Query: 99 DRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFL 158 D++ E + + L G ++ KV Y +G+ + + + G + S + + Sbjct: 28375 DKV*EKKGSIVKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*TVSTKNII 28554 Query: 159 IATGERPR-YLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVT 217 IATG P + G+P D++ +SS +L P K +++GA + LE G VT Sbjct: 28555 IATGSEPSPFPGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVT 28734 Query: 218 VMVRSILLRGF-DQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNS 276 V+ + + F D D+A + ++ HGI+ + I + GT + + Sbjct: 28735 VVEFAD*ICPFLDSDIAKVFHKSLKHHGIEILTGHKVISG*NL--GTHAVVNIEPVKGGP 28908 Query: 277 EEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDIL 336 + E+ V++A GR +G + VGVK+++K G+I D TN+P IYAIGD++ Sbjct: 28909 SISL*AEH--VLVATGRRPFLGGLG*DKVGVKMDDK-GRIITNDNL*TNIPNIYAIGDVV 29079 Query: 337 EDKVELTPVAIQAGR---LLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSE 387 P+ G + A AG +Y+ +P ++T E G +E Sbjct: 29080 AG-----PMLAHKGEEEGIAAVENIAGKAGHVNYDTIPNVIYTHPEIAWVGKNE 29226 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 18,514,724 Number of Sequences: 2017 Number of extensions: 200122 Number of successful extensions: 399 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 32 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 344 Number of HSP's gapped (non-prelim): 49 length of query: 499 length of database: 16,266,656 effective HSP length: 107 effective length of query: 392 effective length of database: 16,050,837 effective search space: 6291928104 effective search space used: 6291928104 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 108 (46.2 bits)