TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|323451867|gb|EGB07743.1| selenoprotein [Aureococcus anophagefferens] (498 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplace... 281 e-108 gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplace... 149 6e-36 gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplace... 142 7e-34 gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplace... 97 8e-27 gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplace... 79 9e-15 gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplace... 65 2e-10 >gi|340502618|gb|GL984165.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251204, whole genome shotgun sequence Length = 85820 Score = 281 bits (718), Expect(2) = e-108 Identities = 160/338 (47%), Positives = 211/338 (62%), Gaps = 5/338 (1%) Frame = -2 Query: 144 LTDKKGNKTSVTAARFIVAVGGRPNKLGCPGGEH--AIDSDDLFSLPKAPGRVLCVGGGY 201 LTDKKG KT +T+ +++VGGRP L I SDD+FSL K PG+ L VG Y Sbjct: 68062 LTDKKGEKTKITSKYILISVGGRPTFLDDIPNNR*LCITSDDIFSL*KPPGKTLIVGASY 67883 Query: 202 IALECGGFTAGMGYPTTCMVRSILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEKDA 261 IALEC GF AG Y T MVRSILLRGFD E KI +++ HGV+ G P+ I+++ Sbjct: 67882 IALECAGFLAGFHYDVTVMVRSILLRGFD*EMANKIGEYMERHGVKFIRGTIPSDIKQEG 67703 Query: 262 ATGVLTVTDSGGGVHE--YDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQ 319 + D+ G YDT+++A GR +DT +G++ + G N K + C D+ Sbjct: 67702 EKKRVFWKDANG*TQSDVYDTIMLAIGRSSDT*NIGLE*L-GIKTKPNGK---IICNDDD 67535 Query: 320 LPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGT 379 V+AIGD V+ RPELTP AI+AG L RRLFGG+ + M+Y + TT+FTPLEYGT Sbjct: 67534 TTSIEGVFAIGDCVDKRPELTPTAIKAGRLLVRRLFGGEKKFMNYHYIPTTVFTPLEYGT 67355 Query: 380 IGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGK-VVGFH 438 IG SE+DAK + G+ N++ + S F PLE+ S+ R + D + KL+++ + K VVGFH Sbjct: 67354 IGFSEEDAKVKFGDANIKVFYSIFKPLEWNYSDHRHD--DKGYCKLIINSADNKRVVGFH 67181 Query: 439 YLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAE 476 YLGPNAGE+T GF+IA AT F VGIHPT AE Sbjct: 67180 YLGPNAGEVT*GFAIAFILKATKEHFDDIVGIHPTSAE 67067 Score = 130 bits (326), Expect(2) = e-108 Identities = 66/135 (48%), Positives = 80/135 (59%) Frame = -1 Query: 10 HGFDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKK 69 H + YD LA +KE A LGAKVA+ DFVKPSP GS WGLGGTCVNVGCIPKK Sbjct: 68531 H*YTYDLFVIGGGSGGLAAAKEAASLGAKVALADFVKPSPIGSKWGLGGTCVNVGCIPKK 68352 Query: 70 LMHTASIIGETLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITY 129 LMH ++++GE+ H WE M V +HIK LN+ Y+VAL E + Y Sbjct: 68351 LMHLSAVVGESHEA*KNMGWSDIKDKYSHQWEPMV*RV*NHIKKLNWGYKVALAENEVKY 68172 Query: 130 LNRLGEFEDKNTISL 144 N L DKNT+ + Sbjct: 68171 FNSLATLFDKNTVEV 68127 >gi|340503816|gb|GL984010.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251048, whole genome shotgun sequence Length = 45969 Score = 149 bits (377), Expect = 6e-36 Identities = 104/285 (36%), Positives = 135/285 (47%), Gaps = 46/285 (16%) Frame = +3 Query: 4 DGFTKEHGFDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPA------------- 50 + E +DYD LA + E KLG K + D+V PS Sbjct: 14208 ENINNETKYDYDVAIIGGGSAGLAFAFEA*KLGLKTIIFDYVVPSKQ****KNEKTYTYL 14387 Query: 51 -------GSTWGLGGTCVNVGCIPKKLMHTASIIGETLXXXXXXXXXXXXXXXXH----- 98 G+ WG+GGTCVNVGCIPK+L HTAS+I + + + Sbjct: 14388 YFIFLKRGT*WGIGGTCVNVGCIPKRLFHTASVIKDNILKANDFGFFDD*NFQNNP*AIS 14567 Query: 99 -DWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLT-DK--------- 147 WE + S + +I LN+K+ L ITY+N L +DKNTI T DK Sbjct: 14568 FSWERLISNI*SYISDLNYKFLF*LNSKEITYINALATLKDKNTILHT*DKN*LKKCIKN 14747 Query: 148 ------KGNKTSVTAARFIVAVGGRPNKLGCPGGEHAI----DSDDLFSLPKAPGRVLCV 197 K NK +TA ++ VGGRP L I SDDLFSL K PG+ L V Sbjct: 14748 QNFEEIKDNK--ITA*YIVICVGGRPKSLKINKK*EKI**MITSDDLFSL*KKPGKTLIV 14921 Query: 198 GGGYIALECGGFTAGMGYPTTCMVRSILLRGFDRECVGKIEAHIK 242 GGGYIA+ECGGF + +G TT + R+ L+ FDRE V I ++K Sbjct: 14922 GGGYIAMECGGFLSNLGI*TTVLTRNGWLKDFDREMV*AIIENMK 15056 Score = 73.2 bits (178), Expect(2) = 7e-17 Identities = 49/123 (39%), Positives = 66/123 (53%), Gaps = 1/123 (0%) Frame = +1 Query: 375 LEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTG-K 433 +EY +G+ E+ A + G N+E Y F PLE L+ + F K++ DKS K Sbjct: 15817 IEYSFVGIHEE*AI*EYGIDNIEVYH*RFLPLED*LTIS*YN***KSFIKIICDKSNNDK 15996 Query: 434 VVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEFTSMTVTKSSGESAAK 493 V+G HYLG NAGEI G+ +A T+ IHPT AEE S ++KSSGE+ K Sbjct: 15997 VLGIHYLGLNAGEIL*GYVVAFN*NLFKK*LN*TITIHPTSAEEILSTNISKSSGENFDK 16176 Query: 494 GGC 496 C Sbjct: 16177 DMC 16185 Score = 33.5 bits (75), Expect(2) = 7e-17 Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 9/55 (16%) Frame = +2 Query: 332 VVEGRPELTPVAIEAGLRLARRLFGGKTE---------PMDYECVATTIFTPLEY 377 +++G PELT A + LA R++G K M+Y+ TT+FTPL + Sbjct: 15623 IIKGVPELTSTA*MSSRLLAHRIYGYKNNLGED*MKNYKMNYKNTPTTLFTPLGF 15787 >gi|340500396|gb|GL984375.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251414, whole genome shotgun sequence Length = 118652 Score = 142 bits (359), Expect = 7e-34 Identities = 84/189 (44%), Positives = 113/189 (59%), Gaps = 17/189 (8%) Frame = -2 Query: 325 HVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECV------------ATTIF 372 ++YAIG+V++G PELTPVA + G LA+RL K D CV TTIF Sbjct: 58840 NIYAIGEVLDGIPELTPVA*KQG*LLAKRLIY-KR*NKDINCVYF*KLKLDVNDFPTTIF 58664 Query: 373 TPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDG----CFAKLVVD 428 TP EY GLSE+ + + GE N+E Y S++ PLE +S E + +AK++ + Sbjct: 58663 TPTEYSCTGLSEE*SIKKYGEENIEVYHSKYTPLEE*ISPRYDEDYNSLQRKAYAKIICN 58484 Query: 429 K-STGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEFTSMTVTKSS 487 K K+VG HYLGPNAGE+ G+++AM+ G T D T+GIHPT +EEFT + +TKSS Sbjct: 58483 KLENDKIVGIHYLGPNAGEVM*GYAVAMKLGTTKFDLDRTIGIHPTTSEEFTGLNITKSS 58304 Query: 488 GESAAKGGC 496 GE K C Sbjct: 58303 GEPYEKTSC 58277 Score = 89.0 bits (219), Expect = 1e-17 Identities = 57/151 (37%), Positives = 80/151 (52%), Gaps = 22/151 (14%) Frame = -3 Query: 100 WESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTDKKGNKTS------ 153 WE++ V +IKS+NF Y+ L + I Y+N L F DKNT+ + K + +S Sbjct: 59979 WENLV*NV*SYIKSINFGYKK*LVDKNIDYVNALATFYDKNTLVFSPKS*HISSYL*DNN 59800 Query: 154 ------------VTAARFIVAVGGRPNKLG---CPGG-EHAIDSDDLFSLPKAPGRVLCV 197 +TA ++AVGGRP L C ++ I SDD+F P + L V Sbjct: 59799 LIN*EK*EDLGKITAD*IVIAVGGRPLILPDE*CKNSYKYGITSDDIFM*KCPPRKTLVV 59620 Query: 198 GGGYIALECGGFTAGMGYPTTCMVRSILLRG 228 GGGYIA+EC GF + +GY T+ M R + LRG Sbjct: 59619 GGGYIAVECAGFLSTLGYHTSMMTR*LYLRG 59527 Score = 56.2 bits (134), Expect = 8e-08 Identities = 22/26 (84%), Positives = 25/26 (96%) Frame = -1 Query: 51 GSTWGLGGTCVNVGCIPKKLMHTASI 76 G+TWGLGGTCVNVGCIPKKLMHT+ + Sbjct: 60194 GNTWGLGGTCVNVGCIPKKLMHTSGL 60117 >gi|340500124|gb|GL984390.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251429, whole genome shotgun sequence Length = 121512 Score = 97.4 bits (241), Expect(2) = 8e-27 Identities = 67/197 (34%), Positives = 89/197 (45%), Gaps = 24/197 (12%) Frame = +3 Query: 58 GTCVNVGCIPKKLMHTASIIGETLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFK 117 GT +NVG I KK MH A+ GE H+W+ + S + ++IK+ ++ Sbjct: 106860 GTNLNVGSIAKKFMHIAA*NGELHN*YVNMGYGDVD*KSNHEWKKLISRI*NYIKNTSW* 107039 Query: 118 YRVALREAGITYLNRLGEFEDKNTIS-----------------------LTDKKGNKTSV 154 Y++ L + Y N +KN I L K G+ V Sbjct: 107040 YKIDL*N*EVKYYNAFASIIEKNKIEVYIINL*ILKNIIKFKYI*YL*KLKYKNGDIEKV 107219 Query: 155 TAARFIVAVGGRPNKLGCPGG-EHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGM 213 TA I+AVG RP L G +H I SDDLF P + L VG Y+ALE GF Sbjct: 107220 TAKFIIIAVGSRPKYLDVEGSPQHVITSDDLF**ENPPNKTLIVGASYVALETAGFLNSF 107399 Query: 214 GYPTTCMVRSILLRGFD 230 GY + MVRS +LRGFD Sbjct: 107400 GYNVSVMVRSKILRGFD 107450 Score = 81.3 bits (199), Expect(2) = 4e-23 Identities = 50/124 (40%), Positives = 65/124 (52%), Gaps = 17/124 (13%) Frame = +3 Query: 392 GECNVESYISEFAPLEYALSETRSERGDGCFAKLVVD-KSTGKVVGFHYLGPNAGEITQG 450 GE NV+ + S F PLE+ S+T E D + KL+V+ K KVVG HYLGP+AGE+ G Sbjct: 108180 GEENVKVFYSIFRPLEWTFSDTNEE--DKGYCKLIVNVKKNNKVVGLHYLGPHAGEVV*G 108353 Query: 451 FSIAMRKGATYADFISTVGIHPTVAE----------------EFTSMTVTKSSGESAAKG 494 +++A T DF GIHPT +E EF M K SG+ A + Sbjct: 108354 YALAFLLNGTKEDFDKAAGIHPTSSEVFKYIIFDFLFKLKKKEFIGMK*FKGSGKMAKES 108533 Query: 495 GCUG 498 GC G Sbjct: 108534 GC*G 108545 Score = 46.6 bits (109), Expect(2) = 4e-23 Identities = 24/43 (55%), Positives = 30/43 (69%) Frame = +1 Query: 345 EAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDA 387 + G LARRLF GK++ M Y + T IF+ LEY TIG SE+DA Sbjct: 108040 KTG*LLARRLFNGKSKLM*YINIPTIIFSSLEYATIGFSEEDA 108168 Score = 42.7 bits (99), Expect(2) = 8e-27 Identities = 22/49 (44%), Positives = 26/49 (53%) Frame = +2 Query: 10 HGFDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGG 58 H DYD L+ + E GAK A+ DFV PSP G+ WGLGG Sbjct: 106607 HKCDYDFFVIGGGCGGLSFA*EAC*FGAKGAIADFVYPSP*GNKWGLGG 106753 >gi|340509195|gb|GL983048.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509249415, whole genome shotgun sequence Length = 81071 Score = 79.3 bits (194), Expect = 9e-15 Identities = 76/281 (27%), Positives = 118/281 (41%), Gaps = 7/281 (2%) Frame = +1 Query: 111 IKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTDKKGNKTSVTAARFIVAVGGRPNKL 170 +K L ++ + Y+ G+ DKNT+ + G K +V+ I+A G P+ Sbjct: 28405 VKGLTSGIEFLFKKNKVDYIKGFGKISDKNTL*VDLSNG*K*TVSTKNIIIATGSEPSPF 28584 Query: 171 -GCPGGEHAI-DSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRG 228 G P E I S +L K P +++ +G G I LE G G T + + + Sbjct: 28585 PGLPFDEKIILSSTGALAL*KIPEKMIIIGAGVIGLEMGSVY*RFGTKVTVVEFAD*ICP 28764 Query: 229 FDRECVGKI-EAHIKHHGVQLQVGVTPAKIEKDAATGVLTVTDSGGGVHEY---DTVLVA 284 F + K+ +KHHG+++ G V+ + GG + VLVA Sbjct: 28765 FLDSDIAKVFHKSLKHHGIEILTGHKVISG*NLGTHAVVNIEPVKGGPSISL*AEHVLVA 28944 Query: 285 TGRYADTAALGIDNVPGASANVNAKTKKLECVDEQL-PGAPHVYAIGDVVEGRPELTPVA 343 TGR LG D V V K ++ L P++YAIGDVV G P L Sbjct: 28945 TGRRPFLGGLG*DKV-----GVKMDDKGRIITNDNL*TNIPNIYAIGDVVAG-PMLAHKG 29106 Query: 344 IEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSE 384 E G+ + GK ++Y+ + I+T E +G +E Sbjct: 29107 EEEGIAAVENI-AGKAGHVNYDTIPNVIYTHPEIAWVGKNE 29226 >gi|340506218|gb|GL983679.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509250595, whole genome shotgun sequence Length = 37305 Score = 64.7 bits (156), Expect = 2e-10 Identities = 71/270 (26%), Positives = 110/270 (40%), Gaps = 12/270 (4%) Frame = +2 Query: 127 ITYLNRLGEFEDKNTISLTDKKGNKT--SVTAARFIVAVGGRPNKLGCPGGEH----AID 180 + Y+ G+ DKNTI++ D N +++ I+A G P PG E + Sbjct: 17951 VDYIKGYGKLLDKNTINV-DLVNN*G**KLSSKNIIIATGSEPTPF--PGLEFDEKTIVS 18121 Query: 181 SDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGF-DRECVGKIEA 239 S SLP P +++ +G G I LE G +G T + + + F D E Sbjct: 18122 STGALSLPVIPEKLIVIGAGVIGLEMGSVY*RLGTKVTVIEYAD*ICPFLDNEIAKAFHK 18301 Query: 240 HIKHHGVQLQVGVTPAKIEKDAATGVLTVTDSGGGVHEY---DTVLVATGRYADTAALGI 296 + +G+++ G G++ V GG + + +LVATGR L Sbjct: 18302 TLTKYGLEILTGHKVTGG*NKGTNGIVNVEPVKGGESKSLSANHILVATGRKPFVKGLNA 18481 Query: 297 DNVPGASANVNAKTKKLECVDEQL--PGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRL 354 + N K V L P++YAIGDVV G P L E G+ + Sbjct: 18482 QEIGIEFDN------KGRIVTNNLL*TNIPNIYAIGDVVAG-PMLAHKGEEEGIAAVENI 18640 Query: 355 FGGKTEPMDYECVATTIFTPLEYGTIGLSE 384 GK +DY+ + I+T E +G +E Sbjct: 18641 -AGKGGHVDYDAIPNVIYTHPEIAWVGKNE 18727 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,575,450 Number of Sequences: 2017 Number of extensions: 102950 Number of successful extensions: 186 Number of sequences better than 1.0e-04: 6 Number of HSP's better than 0.0 without gapping: 19 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 145 Number of HSP's gapped (non-prelim): 28 length of query: 498 length of database: 16,266,656 effective HSP length: 107 effective length of query: 391 effective length of database: 16,050,837 effective search space: 6275877267 effective search space used: 6275877267 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 108 (46.2 bits)