TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium
transferase [Homo sapiens]
         (501 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig19987  1321 1331                                                117   4e-31
Contig8169  4144 7930                                                 121   7e-27
Contig98548  227 227                                                   50   2e-05
Contig6356  7191 13816                                                 32   5.6  
Contig1728  13617 15857                                                32   9.5  

>Contig19987  1321 1331
          Length = 1331

 Score =  117 bits (292), Expect(2) = 4e-31
 Identities = 59/92 (64%), Positives = 70/92 (76%)
 Frame = -3

Query: 251  VNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMY 310
            VNNAYG+Q       I Q A+ GRIDA +QS DKNFMVPVGGAIIAG ++ F+  I+K Y
Sbjct: 1278 VNNAYGLQDHAVCQGITQAAKKGRIDAIIQSTDKNFMVPVGGAIIAGPDEKFMNAIAKNY 1099

Query: 311  PGRASASPSLDVLITLLSLGSNGYKKLLKERK 342
            PGRAS SP LD+ ITL+SLG +GY+KLL ERK
Sbjct: 1098 PGRASGSPVLDLFITLISLGRDGYEKLLHERK 1003



 Score = 39.7 bits (91), Expect(2) = 4e-31
 Identities = 15/30 (50%), Positives = 23/30 (76%)
 Frame = -2

Query: 343 EMFSYLSNQIKKLSEAYNERLLHTPHNPIS 372
           EM++YL  ++  +++ Y ERLL TPHNP+S
Sbjct: 913 EMYNYLKEKMSAIAKKYGERLLLTPHNPVS 824


>Contig8169  4144 7930
          Length = 7930

 Score =  121 bits (304), Expect = 7e-27
 Identities = 84/210 (40%), Positives = 112/210 (53%), Gaps = 47/210 (22%)
 Frame = -3

Query: 40   KCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYR-----FIHG 94
            + PE+GWDE T+E  LH +A MDSNNF+ N GVGEREGRVAS +VA+RHYR     F++ 
Sbjct: 3455 RLPESGWDEGTIEYILHTIAGMDSNNFIKNVGVGEREGRVASGIVAKRHYR*TNYLFLNR 3276

Query: 95   IGRSGDISAVQPKAAGS---------SLLNKITNSLV---------------LDIIKLAG 130
               S  + +   K  G+         S  N   + L+               L I+K   
Sbjct: 3275 H*HSN*VRSWCWKEWGNNRTSAQSCWSFPNF*IDQLLSY*CHACCWYLFFSSLFILKFR* 3096

Query: 131  VH---TVANCFVVPMATGMSLTLCFLTLRHKRP--------------KAKYII-WPRIDQ 172
             +    +    VVP+ATGM+LTL  LTL+ +                K +Y++ WPRIDQ
Sbjct: 3095 FNLGLRIPAAIVVPLATGMALTLTLLTLKQQHDEKQLSDAQIAINDIKNQYVVFWPRIDQ 2916

Query: 173  KSCFKSMITAGFEPVVIENVLEGDELRTDL 202
            KSC KS+ TAGF P+ I   L+GDELRT +
Sbjct: 2915 KSCLKSIHTAGFRPIPIPVKLDGDELRTGM 2826



 Score = 77.8 bits (190), Expect = 1e-13
 Identities = 38/88 (43%), Positives = 58/88 (65%), Gaps = 1/88 (1%)
 Frame = -2

Query: 383 HRDKAVTQLGSMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCA-YLNAASAIG 441
           H  K  T +GSMLF R VSG RV+  G  ++V+G  F GF +HT+ YP + YL AA+A+G
Sbjct: 474 HTKKDATSVGSMLFARNVSGVRVISGGERKSVAGIWFDGFGAHTDKYPFSPYLTAAAAVG 295

Query: 442 MKMQDVDLFIKRLDRCLKAVRKERSKES 469
           M  ++VD+F +R+D+ L +++   +  S
Sbjct: 294 MTKKEVDIFAERIDKVLSSLQPSSATSS 211


>Contig98548  227 227
          Length = 227

 Score = 50.4 bits (119), Expect = 2e-05
 Identities = 22/59 (37%), Positives = 38/59 (64%), Gaps = 3/59 (5%)
 Frame = +1

Query: 198 LRTDLKAVEAKVQELG---PDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNN 253
           + +DL A+E  V++     P   + + +TTSCFAPR PDR++++A +C  Y + H+ N+
Sbjct: 22  ITSDLAALEEAVRKHTTEFPHACVVVMTTTSCFAPRAPDRVDDVATLCQKYGVAHVGNS 198


>Contig6356  7191 13816
          Length = 13816

 Score = 32.3 bits (72), Expect = 5.6
 Identities = 19/55 (34%), Positives = 27/55 (49%)
 Frame = +3

Query: 336  KLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQ 390
            +  K R  +F Y S     +SE    R++H   NP  +    K L+EHR + VTQ
Sbjct: 9027 RTFKNRNTVFLYSSTYFFDISEI---RIVHIFTNPAKIWPKNKNLNEHRHRVVTQ 9182


>Contig1728  13617 15857
          Length = 15857

 Score = 31.6 bits (70), Expect = 9.5
 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 3/41 (7%)
 Frame = +3

Query: 459   KAVRKERSKESD---DNYDKTEDVDIEEMALKLDNVLLDTY 496
             K   K+++K+SD   DN+D TE +DI    LKL N+ L+ Y
Sbjct: 10644 KKSNKQQNKDSDNDNDNFDNTE-IDIASNKLKLININLELY 10763


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.320    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 173,859,559
Number of Sequences: 297657
Number of extensions: 2451361
Number of successful extensions: 11054
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 10418
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 11046
length of query: 501
length of database: 106,339,350
effective HSP length: 118
effective length of query: 383
effective length of database: 71,215,824
effective search space: 27275660592
effective search space used: 27275660592
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 70 (31.6 bits)