TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MSP, [Se user]; [Dictyostelium discoideum AX4
(Eukaryota/Dictyosteliida)]
         (227 letters)

Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig22495 1206 1206                                                  54   6e-07
Contig867 23543 35590                                                  33   0.79 
Contig61064 433 433                                                    32   1.8  
Contig4439 9863 17962                                                  30   8.7  

>Contig22495 1206 1206
          Length = 1206

 Score = 53.9 bits (128), Expect = 6e-07
 Identities = 27/47 (57%), Positives = 32/47 (68%), Gaps = 1/47 (2%)
 Frame = -2

Query: 117 YHFHKCKKRPSDRG-HWIKFGPFYLTAIAVPLATFDILRHILVDNSI 162
           Y +HKC  R   RG HW +FGPFYL  I+VPL   D++RHIL DN I
Sbjct: 182 YVYHKCCLR---RGTHWHRFGPFYLCLISVPLVCADLMRHILEDNGI 51


>Contig867 23543 35590
          Length = 35590

 Score = 33.5 bits (75), Expect = 0.79
 Identities = 20/60 (33%), Positives = 31/60 (51%)
 Frame = +2

Query: 111  LSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFIS 170
            L +L    F K KK  + +   IK       ++A+PL T  +LRH L+  +I  + SF+S
Sbjct: 8933 LLSLRGKKF*KIKKSKNKKLKIIKNTSGVFNSVALPLITVRLLRHSLISTAILALRSFVS 9112


>Contig61064 433 433
          Length = 433

 Score = 32.3 bits (72), Expect = 1.8
 Identities = 11/27 (40%), Positives = 14/27 (51%)
 Frame = -2

Query: 201 GYACLLVGTIWAADLIPKIKKVWTQLR 227
           GY CL +G  W  D   K++  W Q R
Sbjct: 429 GYICLFIGNFWCIDFHLKVRDAWNQRR 349


>Contig4439 9863 17962
          Length = 17962

 Score = 30.0 bits (66), Expect = 8.7
 Identities = 16/45 (35%), Positives = 23/45 (51%)
 Frame = +2

Query: 162   IWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLL 206
             I+   SF+S +   P C N N+   SV     ++ +TYT   CLL
Sbjct: 17663 IYAAVSFVSTSTLNPSCTNGNLGSFSVKCQGGSLPYTYT--VCLL 17791


  Database: /users/rg/didac/GENOMES/P.polycephalum/genome.fa
    Posted date:  Sep 30, 2011 12:44 AM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.321    0.136    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,945,116
Number of Sequences: 297657
Number of extensions: 1205798
Number of successful extensions: 6961
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6691
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6960
length of query: 227
length of database: 106,339,350
effective HSP length: 110
effective length of query: 117
effective length of database: 73,597,080
effective search space: 8610858360
effective search space used: 8610858360
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 66 (30.0 bits)
