TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MSP, [Se user]; [Dictyostelium discoideum AX4
(Eukaryota/Dictyosteliida)]
         (227 letters)

Database: /users/rg/didac/GENOMES/I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GL983849.1| Ichthyophthirius multifiliis unplaced genomic sca...    29   3.6  

>gb|GL983849.1| Ichthyophthirius multifiliis unplaced genomic scaffold
              scaff_1120509250885, whole genome shotgun sequence
          Length = 253131

 Score = 29.3 bits (64), Expect = 3.6
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
 Frame = +3

Query: 163    WTIHSFISPAAYR-PGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKI 219
              W   SFIS  +   P      I  +     FS+I+  + GY C +  TIW  D++P +
Sbjct: 223980 WIYESFISSISIGFPFSF*RIINSIDAFIVFSSIIIEFCGYKCRMF*TIW--DILPNV 224147


  Database: /users/rg/didac/GENOMES/I.multifiliis_strain_G5/genome.fa
    Posted date:  Sep 29, 2011 11:36 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.321    0.136    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,936,388
Number of Sequences: 2017
Number of extensions: 102616
Number of successful extensions: 197
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 181
Number of HSP's gapped (non-prelim): 22
length of query: 227
length of database: 16,266,656
effective HSP length: 99
effective length of query: 128
effective length of database: 16,066,973
effective search space: 2056572544
effective search space used: 2056572544
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
