TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MSP, [Se user]; [Dictyostelium discoideum AX4
(Eukaryota/Dictyosteliida)]
         (227 letters)

Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2, whole ...   367   e-102
gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole ...    33   0.23 
gb|CM000153.2| Dictyostelium discoideum AX4 chromosome 4, whole ...    27   9.7  

>gb|CM000151.3| Dictyostelium discoideum AX4 chromosome 2, whole genome shotgun sequence
          Length = 8484197

 Score =  367 bits (941), Expect = e-102
 Identities = 194/270 (71%), Positives = 194/270 (71%), Gaps = 43/270 (15%)
 Frame = -1

Query: 1       MSLFNLPKVDLGEDCEGGVXARP-----------DESTPLISKTNDEEKANIGISSTSNS 49
               MSLFNLPKVDLGEDCEGGV ARP           DESTPLISKTNDEEKANIGISSTSNS
Sbjct: 7225967 MSLFNLPKVDLGEDCEGGV*ARPSSSSSSINNASDESTPLISKTNDEEKANIGISSTSNS 7225788

Query: 50      PQEEQTKKPXXXXXXXXXXXXPALVG--------------------------------SX 77
               PQEEQTKKP            PALVG                                S 
Sbjct: 7225787 PQEEQTKKPLFISILTLLISIPALVGKFLILIIIIKSIK*CYEN*YIYIYIFILIFIGS* 7225608

Query: 78      CWPXXXXXXXXXXXXXXXXXXXHSLTFAITLSILSNLAQYHFHKCKKRPSDRGHWIKFGP 137
               CWP                   HSLTFAITLSILSNLAQYHFHKCKKRPSDRGHWIKFGP
Sbjct: 7225607 CWPVLIASLSGVAVSAGSVELAHSLTFAITLSILSNLAQYHFHKCKKRPSDRGHWIKFGP 7225428

Query: 138     FYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVF 197
               FYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVF
Sbjct: 7225427 FYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVF 7225248

Query: 198     TYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
               TYTGYACLLVGTIWAADLIPKIKKVWTQLR
Sbjct: 7225247 TYTGYACLLVGTIWAADLIPKIKKVWTQLR 7225158



 Score = 28.9 bits (63), Expect = 3.3
 Identities = 16/46 (34%), Positives = 24/46 (52%)
 Frame = +3

Query: 131     HWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRP 176
               H   FG  Y   ++ PL T++ +  +L  N IW  H FI   +Y+P
Sbjct: 1639386 HT*NFGT*YSPLLSSPL*TYE*ISRLLNQN-IWKHHPFIVIKSYKP 1639520


>gb|CM000154.2| Dictyostelium discoideum AX4 chromosome 5, whole genome shotgun sequence
          Length = 5125352

 Score = 32.7 bits (73), Expect = 0.23
 Identities = 23/73 (31%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
 Frame = +3

Query: 154     RHILVDNSIWTIHSFISPA-AYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWA 212
               R+ ++ N+ +++ S I  A A +      +I C     W+S  +F      CLL+GTI  
Sbjct: 1309914 RYFIIFNTGFSLTSLIIAASASKFEAGTGSIECWIRDRWYSICLFWLPCGICLLIGTICI 1310093

Query: 213     ADLIPKIKKVWTQ 225
               A +I +I KV+ Q
Sbjct: 1310094 ASVIVEIYKVFFQ 1310132


>gb|CM000153.2| Dictyostelium discoideum AX4 chromosome 4, whole genome shotgun sequence
          Length = 5450249

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +3

Query: 170     SPAAYRPGCENENITCLSVMGWFSAIVFTYTGY 202
               +PAA R  C+N    C S+  +    + T+TGY
Sbjct: 4265151 TPAAKRKDCDNNFDNCESLYFYQKKFIGTFTGY 4265249


  Database: /users/rg/didac/GENOMES/D.discoideum_AX4/genome.fa
    Posted date:  Sep 29, 2011  8:31 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.321    0.136    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,856,507
Number of Sequences: 6
Number of extensions: 106453
Number of successful extensions: 436
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 376
Number of HSP's gapped (non-prelim): 98
length of query: 227
length of database: 11,314,357
effective HSP length: 97
effective length of query: 130
effective length of database: 11,313,775
effective search space: 1470790750
effective search space used: 1470790750
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
