BLASTP 2.2.16 [Mar-25-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|256541943|gb|CM000151.3|:subseq(7223000,4000) Dictyostelium
discoideum AX4 chromosome 2, whole genome shotgun
sequence:[translate(1)]
         (227 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
           16,352,912 sequences; 1,327,212,733 total letters

Searching... please wait.. done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_642828.2| membrane selenoprotein [Dictyostelium discoideu...   384   e-134
ref|XP_003284027.1| hypothetical protein DICPUDRAFT_52650 [Dicty...   254   7e-84
gb|EFA83018.1| membrane selenoprotein [Polysphondylium pallidum ...   191   3e-59
gb|EGG17044.1| membrane selenoprotein [Dictyostelium fasciculatum]    175   8e-53
ref|XP_002947604.1| membrane selenoprotein [Volvox carteri f. na...   118   4e-30
ref|XP_002508818.1| predicted protein [Micromonas sp. RCC299] >g...   101   2e-24
ref|XP_001415402.1| predicted protein [Ostreococcus lucimarinus ...    87   4e-19
ref|XP_003060097.1| predicted protein [Micromonas pusilla CCMP15...    67   9e-11
ref|XP_002773820.1| hypothetical protein Pmar_PMAR011664 [Perkin...    58   1e-07
ref|XP_002768511.1| hypothetical protein Pmar_PMAR022894 [Perkin...    44   0.008
gb|EFW42499.1| nuclear localization sequence binding protein [Ca...    36   4.8  
ref|XP_001646943.1| hypothetical protein Kpol_2000p52 [Vanderwal...    35   8.9  

>ref|XP_642828.2| membrane selenoprotein [Dictyostelium discoideum AX4]
 sp|Q86KP5|MSP_DICDI RecName: Full=Membrane selenoprotein
 gb|EAL69009.2| membrane selenoprotein [Dictyostelium discoideum AX4]
          Length = 243

 Score =  384 bits (985), Expect = e-134
 Identities = 194/238 (81%), Positives = 195/238 (81%), Gaps = 11/238 (4%)

Query: 1   MSLFNLPKVDLGEDCE-----------ASINNASDESTPLISKTNDEEKANIGISSTSNS 49
           MSLFNLPKVDLGEDCE           +SINNASDESTPLISKTNDEEKANIGISSTSNS
Sbjct: 1   MSLFNLPKVDLGEDCEGGVUARPSSSSSSINNASDESTPLISKTNDEEKANIGISSTSNS 60

Query: 50  PQEEQTKKPXXXXXXXXXXXXPALVGSXCWPXXXXXXXXXXXXXXXXXXXHSLTFAITLS 109
           PQEEQTKKP            PALVGS CWP                   HSLTFAITLS
Sbjct: 61  PQEEQTKKPLFISILTLLISIPALVGSUCWPVLIASLSGVAVSAGSVELAHSLTFAITLS 120

Query: 110 ILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFI 169
           ILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFI
Sbjct: 121 ILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFI 180

Query: 170 SPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
           SPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR
Sbjct: 181 SPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 238


>ref|XP_003284027.1| hypothetical protein DICPUDRAFT_52650 [Dictyostelium purpureum]
 gb|EGC39469.1| hypothetical protein DICPUDRAFT_52650 [Dictyostelium purpureum]
          Length = 201

 Score =  254 bits (649), Expect = 7e-84
 Identities = 132/230 (57%), Positives = 158/230 (68%), Gaps = 36/230 (15%)

Query: 1   MSLF-NLPKVDLGEDCEASINNASDESTPLISKTNDEEKANIGISST--SNSPQEEQTKK 57
           MS F NLPKV++G+DCE                T+DEEK +I  SS+  S+ PQEEQ+KK
Sbjct: 1   MSFFKNLPKVEVGDDCEGG--------------TSDEEK-DIACSSSPSSDQPQEEQSKK 45

Query: 58  PXXXXXXXXXXXXPALVGSXCWPXXXXXXXXXXXXXXXXXXXHSLTFAITLSILSNLAQY 117
           P            PALVG+                       H++T+AITL+ILSNLAQY
Sbjct: 46  PLIISILTVLISIPALVGA------------------SVTFAHAITYAITLAILSNLAQY 87

Query: 118 HFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPG 177
           H+HKC+ RP ++GHW K+GPFYLT +AVPLATFDILRHILVDN +WT+HSF+SPAAYRPG
Sbjct: 88  HYHKCQSRPREKGHWHKYGPFYLTLLAVPLATFDILRHILVDNQLWTVHSFVSPAAYRPG 147

Query: 178 CENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
           C +ENI CLSVMGWFSAI+FTYTGYA LL GTIW A+L  KIKK+W QLR
Sbjct: 148 CTHENIRCLSVMGWFSAIIFTYTGYALLLTGTIWCAELHIKIKKLWQQLR 197


>gb|EFA83018.1| membrane selenoprotein [Polysphondylium pallidum PN500]
          Length = 195

 Score =  191 bits (486), Expect = 3e-59
 Identities = 87/128 (67%), Positives = 102/128 (79%)

Query: 100 HSLTFAITLSILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVD 159
           H++TFAI+L+ILSNL+QYH+HKC  R SD+GHW KFGPFYLTA+AVP AT DILRHILVD
Sbjct: 65  HAITFAISLTILSNLSQYHYHKCCTRRSDKGHWYKFGPFYLTAVAVPFATVDILRHILVD 124

Query: 160 NSIWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKI 219
           N +WT  S  SPAAYRPGC +E++ CLS  GWF  +  TY GY  L+VGTIW ADL  KI
Sbjct: 125 NGLWTTKSRWSPAAYRPGCNSESMKCLSATGWFFVVFCTYFGYILLMVGTIWCADLHLKI 184

Query: 220 KKVWTQLR 227
           KK+W QLR
Sbjct: 185 KKLWKQLR 192


>gb|EGG17044.1| membrane selenoprotein [Dictyostelium fasciculatum]
          Length = 188

 Score =  175 bits (443), Expect = 8e-53
 Identities = 78/117 (66%), Positives = 89/117 (76%)

Query: 111 LSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFIS 170
           ++NLAQYHFHKC +R SDRGHW KFGPFYLTAIA PLAT DILRHILVDN +WT  +  S
Sbjct: 1   MTNLAQYHFHKCTQRASDRGHWHKFGPFYLTAIAAPLATIDILRHILVDNGLWTPQAHWS 60

Query: 171 PAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
           P AYRPGC +  I CL+  GWF  I  TYTGY  L++GT+W ADL  KI+K W QLR
Sbjct: 61  PVAYRPGCTSATIKCLTPTGWFFVIFCTYTGYILLMIGTVWCADLHTKIRKAWRQLR 117


>ref|XP_002947604.1| membrane selenoprotein [Volvox carteri f. nagariensis]
 gb|EFJ51137.1| membrane selenoprotein [Volvox carteri f. nagariensis]
          Length = 244

 Score =  118 bits (296), Expect = 4e-30
 Identities = 63/161 (39%), Positives = 87/161 (54%), Gaps = 9/161 (5%)

Query: 71  PALVGSXCWPXXXXXXXXXXXXXXXXXXXHSLTFAITLSILSNLAQYHFHKCKKRPSDRG 130
           PALVGS CWP                   H+ + A+TL+IL+NLAQY   K   R     
Sbjct: 88  PALVGSUCWPLLLAGFMGITATAGAKAFSHTFSLALTLAILTNLAQYTAWKSTSRSGS-- 145

Query: 131 HWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPGCEN----ENITCL 186
           HW ++GP +L  IA PL   D++RH L D++IWT  S      YRP C          CL
Sbjct: 146 HWQRYGPAWLLVIATPLMCADLVRHCLQDSAIWTGPS---SRMYRPHCGPVSGFHGFWCL 202

Query: 187 SVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
           SV GW  +I+FTY+G+  ++   +W+++LI K++  WT LR
Sbjct: 203 SVTGWLFSIIFTYSGFVLMVAAVLWSSNLIGKLRVAWTGLR 243


>ref|XP_002508818.1| predicted protein [Micromonas sp. RCC299]
 gb|ACO70076.1| predicted protein [Micromonas sp. RCC299]
          Length = 144

 Score =  101 bits (251), Expect = 2e-24
 Identities = 51/124 (41%), Positives = 75/124 (60%), Gaps = 7/124 (5%)

Query: 100 HSLTFAITLSILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVD 159
           HS++F +TL++L+N+AQ    K K R   R  W K GPF LT  +VPL   D+ RH+L D
Sbjct: 19  HSVSFGVTLAVLTNIAQMVHWKSKTRGGTR--WNKRGPFLLTLASVPLVMLDLTRHVLQD 76

Query: 160 NSIWTIHSFISPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKI 219
              W   S      YRPGC + ++ CLS +G  + ++ TY G+ACL+ G +W+AD+  K+
Sbjct: 77  GGAWIEGS----RMYRPGCGHADVRCLSGIG-ATCLLATYVGFACLISGVLWSADVTRKL 131

Query: 220 KKVW 223
           +  W
Sbjct: 132 RDGW 135


>ref|XP_001415402.1| predicted protein [Ostreococcus lucimarinus CCE9901]
 gb|ABO93694.1| predicted protein [Ostreococcus lucimarinus CCE9901]
          Length = 121

 Score = 86.7 bits (213), Expect = 4e-19
 Identities = 44/98 (44%), Positives = 62/98 (63%), Gaps = 6/98 (6%)

Query: 123 KKRPSDRGH-WIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYRPGCENE 181
           +K    RG+ W  +GP   TA +V L   D +RH+L D+ +WT +S    A YRPGCE+ 
Sbjct: 12  RKARGRRGNAWRVYGPTATTAASVALLCADPMRHVLQDHELWTTNS----AMYRPGCEHG 67

Query: 182 NITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKI 219
           +I CLSV+GW   +  TY G+ACL+VG +W AD + K+
Sbjct: 68  DIRCLSVVGWV-FLTCTYLGFACLIVGALWNADALGKL 104


>ref|XP_003060097.1| predicted protein [Micromonas pusilla CCMP1545]
 gb|EEH56049.1| predicted protein [Micromonas pusilla CCMP1545]
          Length = 212

 Score = 66.6 bits (161), Expect = 9e-11
 Identities = 40/116 (34%), Positives = 58/116 (50%), Gaps = 10/116 (8%)

Query: 112 SNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISP 171
           S LAQ    K  +R   R    + GP  L A ++PL   D+ RH+L D  + +       
Sbjct: 104 STLAQALHWKSSRRRGFR----RRGPIALAAASIPLVMLDLTRHVLQDGGVIS-----DV 154

Query: 172 AAYRPGCENENITCLSVMGWFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQLR 227
             YR GC + ++ CLSV+G   A   TY G+ACLL GT+W A  +   ++   + R
Sbjct: 155 GMYRAGCAHADVRCLSVVGAACATA-TYLGFACLLAGTLWDAGAVTNARRALREHR 209


>ref|XP_002773820.1| hypothetical protein Pmar_PMAR011664 [Perkinsus marinus ATCC 50983]
 gb|EER05636.1| hypothetical protein Pmar_PMAR011664 [Perkinsus marinus ATCC 50983]
          Length = 214

 Score = 57.8 bits (138), Expect = 1e-07
 Identities = 42/167 (25%), Positives = 71/167 (42%), Gaps = 41/167 (24%)

Query: 100 HSLTFAITLSILSNLAQYHFHKCKKRPSD-RGHWIKFGPFYLTAIAVPLATFDILRHILV 158
           H+LTF + L+I +NL Q+ +   K R    + H  K+GPFY   IA      D+ RH+L+
Sbjct: 36  HTLTFGLNLAIFTNLCQFVYAGTKSRRKHIKPHVRKWGPFYCVLIATIGVMMDLTRHVLI 95

Query: 159 DNSIWTIHSFISPAAYR---PGCENE-------------------NITCLSVMG------ 190
           D++ W +    +    +    GC+                      +T  + +G      
Sbjct: 96  DSNNWFMTDVANGDRVKFNFDGCKYRIERVEGTPGECADIGRGPLQVTEENALGVSAPMY 155

Query: 191 -----------WFSAIVFTYTGYACLLVGTIWAADLIPKIKKVWTQL 226
                      WF+ +V T+TG+  + +G +W  DL  K+   W Q+
Sbjct: 156 NPDGSYSFYGIWFT-LVATWTGFLMMFIGILWYTDLPHKLALQWRQV 201


>ref|XP_002768511.1| hypothetical protein Pmar_PMAR022894 [Perkinsus marinus ATCC 50983]
 gb|EER01229.1| hypothetical protein Pmar_PMAR022894 [Perkinsus marinus ATCC 50983]
          Length = 187

 Score = 43.9 bits (102), Expect = 0.008
 Identities = 18/53 (33%), Positives = 29/53 (54%)

Query: 123 KKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIWTIHSFISPAAYR 175
           +KR   + H  K+GPFY+  +A      D+LRH+L+D   W +   I+   Y+
Sbjct: 18  RKRKDVKPHLRKWGPFYMILVATVAVMMDLLRHVLMDAESWKLQDHITGKEYK 70


>gb|EFW42499.1| nuclear localization sequence binding protein [Capsaspora
          owczarzaki ATCC 30864]
          Length = 321

 Score = 36.2 bits (82), Expect = 4.8
 Identities = 21/57 (36%), Positives = 31/57 (54%), Gaps = 6/57 (10%)

Query: 7  PKVDLGEDCEA------SINNASDESTPLISKTNDEEKANIGISSTSNSPQEEQTKK 57
          P+V LG+  EA          A DE+TP   ++ DE  ++    S S+S +EE+TKK
Sbjct: 37 PEVTLGQLFEAYQARTSQKRKAEDEATPAAKRSKDESDSDSDSDSDSDSEEEEETKK 93


>ref|XP_001646943.1| hypothetical protein Kpol_2000p52 [Vanderwaltozyma polyspora DSM
          70294]
 gb|EDO19085.1| hypothetical protein Kpol_2000p52 [Vanderwaltozyma polyspora DSM
          70294]
          Length = 356

 Score = 35.4 bits (80), Expect = 8.9
 Identities = 16/45 (35%), Positives = 30/45 (66%), Gaps = 2/45 (4%)

Query: 10 DLGEDCEASINNASDESTPLISKTNDEEKANIGISSTSNSPQEEQ 54
          DLG D ++S+  +SDE +  +  ++DEE+AN+  +  SN+  ++Q
Sbjct: 50 DLGTDTDSSLGKSSDEES--LGTSDDEEQANVDENGNSNAKSKKQ 92


  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF excluding environmental samples
  from WGS projects
    Posted date:  Nov 28, 2011  1:35 PM
  Number of letters in database: 1,327,212,733
  Number of sequences in database:  16,352,912
  
Lambda     K      H
   0.323    0.136 5.30e-313 

Gapped
Lambda     K      H
   0.267   0.0410 5.30e-313 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 16352912
Number of Hits to DB: 224047145
Number of extensions: 7990167
Number of successful extensions: 16421
Number of sequences better than 10: 0
Number of HSP's better than 10 without gapping: 0
Number of HSP's gapped: 16504
Number of HSP's successfully gapped: 0
Length of query: 227
Length of database: 5622180029
Length adjustment: 135
Effective length of query: 92
Effective length of database: 7709504205
Effective search space: 314137395628
Effective search space used: 314137395628
T: 11
A: 40
X1: 16 (7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.4 bits)
S2: 79 (35.0 bits)

