TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Fep15_danio_rerio
         (138 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig2448  14659 25538                                                29   4.9  
Contig415  22514 32624                                                 29   6.4  

>Contig2448  14659 25538
          Length = 25538

 Score = 29.3 bits (64), Expect = 4.9
 Identities = 26/79 (32%), Positives = 33/79 (41%), Gaps = 13/79 (16%)
 Frame = +3

Query: 2     WLTLVALLALCATGRTAENLSES-----TTDQDKLVIARGKL--------VAPSVVGXSI 48
             W TLV L+ LCA G   E L            D  V+ RG L        V   V    +
Sbjct: 12786 WTTLVMLVVLCAVGIGVEWLRGCVGYCVVMRGDCCVVMRGLLHGDRGDCCVVLRVCALRV 12965

Query: 49    KKMPELYNFLMERWALYHN 67
              ++  L  F M+RWA YH+
Sbjct: 12966 LRVCALCAF-MQRWATYHS 13019


>Contig415  22514 32624
          Length = 32624

 Score = 28.9 bits (63), Expect = 6.4
 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
 Frame = +2

Query: 52    PELYNFLMERWALYHNLEYDSGEDKN-----PRLIFYNDKDEEVKVVPVKKMKADEI 103
             PEL       W  Y NLE+  G D+       R + YND  E++  +  KK++  +I
Sbjct: 27917 PELITEKKNIWLAYLNLEHQFGSDETVSRLLSRAVAYND-PEDISKLSWKKVRRGDI 28084


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.316    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,879,270
Number of Sequences: 297657
Number of extensions: 509472
Number of successful extensions: 1862
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1861
length of query: 138
length of database: 106,339,350
effective HSP length: 101
effective length of query: 37
effective length of database: 76,275,993
effective search space: 2822211741
effective search space used: 2822211741
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)