TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sps2_D.Rerio
         (447 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   109   6e-24
gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   101   1e-21

>gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun
              sequence
          Length = 938756

 Score =  109 bits (272), Expect = 6e-24
 Identities = 104/391 (26%), Positives = 172/391 (43%), Gaps = 41/391 (10%)
 Frame = +1

Query: 28     LNPEEHGLDRGF--RLTAFSDMKGUGCKVPQETLLKLLQGLEPDRPPXXXXXXXXXXXXX 85
              + P     + GF  +LT+ +   G GCK+  +TL  +L  + P +P              
Sbjct: 697924 VEPSFSAENEGFNVKLTSTTHGLGCGCKIRAQTLSSILSKI-PKQP-------------- 698058

Query: 86     XADFGLVSAAQGPRLGIGM---DSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACAN 142
                          P + +G    D C +      L+L  T DFF P+V+ P   G IACAN
Sbjct: 698059 ----------FDPNVLVGTETSDDCGVYKLTSELALCATVDFFTPIVDSPKDFGAIACAN 698208

Query: 143    VLSDLYAMGITECDNMLMLLSVSQKMNEKERDLVLPLMMKGFRDAAEEGGTSVTGGQTVI 202
               LSD+YAMG  EC   L +++   K    +   +L L+++G ++ A E G ++ GG ++ 
Sbjct: 698209 ALSDVYAMG-GECKVALNIVAFPVKRLSLD---ILQLILQGAQEKASEAGVTIIGGHSIE 698376

Query: 203    NPWIIIGGVASVVCQPNDFILPDGAVPGDVLVLTKPLGTQVAVNAHQWLDIPEKWNKIKL 262
              +     G   S +  P       G   GDVL+LTKP+GT V   A           K  L
Sbjct: 698377 DNEPKFGMCVSGLVHPERIWKNVGCRNGDVLLLTKPIGTGVLTTA----------MKKGL 698526

Query: 263    VISREEVEQAYQEAMLNMATLNRTAAAL---MHKFNA-------HAATDITGFGIIGHAR 312
              +   ++  +   E    M+TLN++A+ +   +H+ N+       HA TDITGFG++GH R
Sbjct: 698527 IGPNDDCSKKCIEW---MSTLNKSASEILRGLHRVNSSSTPYEIHACTDITGFGLMGHLR 698697

Query: 313    NL----------AKQQKNDVAFV----------------IHNLPIISKMAAISKAGGNLF 346
               +          A+ +   V F+                I+N+  + K+   +++   + 
Sbjct: 698698 EMITGDNQDNLYAQIESEKVPFMDKAYEMILSDIIPGGTINNMNFVQKITKFNESVSQIV 698877

Query: 347    GLLQGTSSETSGGLLICLPREQAARFCAEMK 377
                L    ++TSGGLL  +   ++    ++ K
Sbjct: 698878 RTLL-CDAQTSGGLLFSVSPHESEEIISDFK 698967


>gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome
             shotgun sequence
          Length = 371401

 Score =  101 bits (252), Expect = 1e-21
 Identities = 77/281 (27%), Positives = 131/281 (46%), Gaps = 4/281 (1%)
 Frame = -2

Query: 40    RLTAFSDMKGUGCKVPQETLLKLLQGLEPDRPPXXXXXXXXXXXXXXADFGLVSAAQGPR 99
             +LT  +   G GCK+    L  +LQ L P +P                        +   
Sbjct: 75705 KLTQLASGLGCGCKINSRLLDSILQKL-PKQP------------------------ENNS 75601

Query: 100   LGIGMDSC---VIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECD 156
             + +G  +C    +   +  +++V T DFF P+V++PY  G IACAN LSD+YAMG     
Sbjct: 75600 VLVGNSTCDDAAVYKINDHVAIVSTIDFFAPIVDNPYDFGAIACANALSDVYAMGGKPQT 75421

Query: 157   NMLMLLSVSQKMNEKERDLVLPLMMKGFRDAAEEGGTSVTGGQTVINPWIIIGGVASVVC 216
              + ++   S+ +  +    +L  +++G  D A E G ++ GG ++ +     G   + + 
Sbjct: 75420 ALSVVAFPSRTLPIE----ILQEILRGSIDKANEAGVAIVGGHSIDDEEPKFGMSVNGII 75253

Query: 217   QPNDFILPDGAVPGDVLVLTKPLGTQVAVNAHQWLDIPEKWNKIKLVISREEVEQAYQEA 276
              P+      G   GD+L LTK +GT V  +A +  ++P +              +  Q A
Sbjct: 75252 HPDKIFRNVGCEEGDLLFLTKRIGTGVITSAMKLGNVPYE-------------SETAQSA 75112

Query: 277   MLNMATLNR-TAAALMHKFNAHAATDITGFGIIGHARNLAK 316
             + +M TLNR T+  L    +  + TD+TGFG++GH + + K
Sbjct: 75111 INSMKTLNRDTSYVLREMKSVSSCTDVTGFGLLGHLKEIMK 74989


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.320    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,990,552
Number of Sequences: 784
Number of extensions: 194070
Number of successful extensions: 627
Number of sequences better than 1.0e-03: 2
Number of HSP's better than  0.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 572
Number of HSP's gapped (non-prelim): 146
length of query: 447
length of database: 13,654,695
effective HSP length: 105
effective length of query: 342
effective length of database: 13,572,375
effective search space: 4641752250
effective search space used: 4641752250
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 98 (42.4 bits)
