TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|81912790|sp|Q80VC6.2|TSAP1_MOUSE RecName: Full=tRNA
selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName:
Full=tRNA selenocysteine-associated protein 1 . Mus musculus
         (287 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   144   7e-35
gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    55   6e-08
gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    54   1e-07
gb|GG738960.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    43   3e-04
gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    42   7e-04

>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun
              sequence
          Length = 707703

 Score =  144 bits (364), Expect = 7e-35
 Identities = 85/189 (44%), Positives = 116/189 (61%), Gaps = 17/189 (8%)
 Frame = -1

Query: 2      AASLWMGDLEPYMDENFISRAFAT-MGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK 60
              A +LWMGDL+  MD+ ++ + FA  +  ++ SVKII++R TG   GY FVEFA +  A+ 
Sbjct: 463674 ARTLWMGDLDDSMDDEYVKKLFANDIRPSINSVKIIKDRNTGKSIGYGFVEFASIEIAKA 463495

Query: 61     CLHKINGKPLPGATPAKRFKLNYAT--------YGKQPDNSPE-------YSLFVGDLTP 105
               L    GKP+P   P K ++LN+A         +  QP   P         S+FVGDL P
Sbjct: 463494 VLESYAGKPIP-TLPNKIYRLNWAAQNQGSNPLFSSQPGGKPSSGGKENIVSIFVGDLAP 463318

Query: 106    DVDDGMLYEFFVKVYPSCRGGKVVLDP-TGVSKGYGFVKFTDELEQKRALTECQGAVGLG 164
              DV+D ML + F   YPS RG KVV+DP +G+SKGYGFVKF DE +  R++TE QG V + 
Sbjct: 463317 DVNDYMLEQTFRNRYPSVRGAKVVMDPKSGISKGYGFVKFADEDDMMRSMTEMQG-VYIS 463141

Query: 165    CKPVRLSVA 173
               +PV++S A
Sbjct: 463140 SRPVKISHA 463114


>gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun
              sequence
          Length = 941567

 Score = 55.5 bits (132), Expect = 6e-08
 Identities = 55/182 (30%), Positives = 84/182 (46%), Gaps = 5/182 (2%)
 Frame = +3

Query: 3      ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62
              A++++G+L+  +DE  +   F  MG  V+  KI R+R+T   +GY FVEF     A   +
Sbjct: 787725 ATIFVGNLDQQVDEELLWELFVQMGP-VVDAKIPRDRITNTHSGYAFVEFKHEHDANYAI 787901

Query: 63     HKINGKPLPGATPAKRFKLNYATYGKQPDN-SPEYSLFVGDLTP--DVDDGMLYEFFVKV 119
                +N   L G    +  KLN     K   N     +L+VG+L P    D+G+L E F + 
Sbjct: 787902 QVMNQIKLFG----RPMKLNRYDQDKSAKNLDVGANLWVGNLDPVGVSDEGILRELFGQF 788069

Query: 120    YPSCRG-GKVVLDP-TGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLSVAIPKA 177
                  +   ++  DP T  SKG+ FV + D  E   A         +  KP+ +  A    
Sbjct: 788070 GVMIQNTPRIQRDPETMESKGFAFVSY-DNFESADAAKMHLNGQYISGKPIIVEYAFKPN 788246

Query: 178    SR 179
              SR
Sbjct: 788247 SR 788252


>gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_47, whole genome shotgun
              sequence
          Length = 339217

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 48/188 (25%), Positives = 87/188 (46%), Gaps = 8/188 (4%)
 Frame = -2

Query: 2      AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61
              +ASL++GDL   + E  +   F  +G  ++S++I R+ +T    GY +V F +   AE+ 
Sbjct: 283668 SASLYVGDLHEEISEALLFDLFHEVG-PIISIRICRDAMTRKSLGYAYVNFQNPQDAERA 283492

Query: 62     LHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFF----- 116
              L  +N   + G       ++ ++        S   ++F+ +L   +D+  LY+ F     
Sbjct: 283491 LDTLNYASVKGIP----IRIMWSQRDPSIRKSGIGNIFIKNLDKSIDNKALYDTFSAFGN 283324

Query: 117    ---VKVYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLSVA 173
                  KV  + + G    D    S GYGFV F  +   ++A+ +  G + L  K V +   
Sbjct: 283323 ILSCKVCQATKKGDNGGDNV-ESAGYGFVHFETQEAAEKAIAKINGML-LNGKQVFVGPF 283150

Query: 174    IPKASRVK 181
              + K  R+K
Sbjct: 283149 VKKTERLK 283126



 Score = 53.9 bits (128), Expect = 2e-07
 Identities = 52/193 (26%), Positives = 90/193 (46%), Gaps = 12/193 (6%)
 Frame = -2

Query: 4      SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63
              ++++ +L+  +DE  +S  F+  GE + +  ++R    G    + F+ FAD A+A   + 
Sbjct: 283098 NIYVKNLDASVDEKELSEVFSKFGE-IQNAVVMRGE-NGASKEFGFINFADHASALIAID 282925

Query: 64     KINGKPLPGAT---------PAKRFKLNYATYGKQPDNSPEY---SLFVGDLTPDVDDGM 111
              ++N K                 +R KL       + +   +Y   +L+V +L   VDD  
Sbjct: 282924 EMNEKDFKNKKLFVGRAQKKNERRAKLKEYFQKLKQEKVNKYKGLNLYVKNLDDSVDDER 282745

Query: 112    LYEFFVKVYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRALTECQGAVGLGCKPVRLS 171
              L + F K +      KV+ D    S+G+GFV F       +ALTE  G + +G KP+ ++
Sbjct: 282744 LRQEFSK-FGDITSAKVMSD-NKQSRGFGFVCFKTPEAANKALTEMSGHM-IGSKPLYVN 282574

Query: 172    VAIPKASRVKPVE 184
               A PK  R   +E
Sbjct: 282573 FAQPKELRRSQLE 282535



 Score = 45.8 bits (107), Expect = 5e-05
 Identities = 42/161 (26%), Positives = 69/161 (42%), Gaps = 8/161 (4%)
 Frame = -2

Query: 4      SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGI-------PAGYCFVEFADLA 56
              ++++ +L+  +D   +   F+  G  ++S K+ +    G         AGY FV F    
Sbjct: 283398 NIFIKNLDKSIDNKALYDTFSAFGN-ILSCKVCQATKKGDNGGDNVESAGYGFVHFETQE 283222

Query: 57     TAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEY-SLFVGDLTPDVDDGMLYEF 115
               AEK + KING  L G        +      K   N   + +++V +L   VD+  L E 
Sbjct: 283221 AAEKAIAKINGMLLNGKQVFVGPFVKKTERLKILSNEDSFTNIYVKNLDASVDEKELSEV 283042

Query: 116    FVKVYPSCRGGKVVLDPTGVSKGYGFVKFTDELEQKRALTE 156
              F K +   +   V+    G SK +GF+ F D      A+ E
Sbjct: 283041 FSK-FGEIQNAVVMRGENGASKEFGFINFADHASALIAIDE 282922


>gb|GG738960.1| Naegleria gruberi genomic scaffold NAEGRscaffold_116, whole genome
             shotgun sequence
          Length = 18323

 Score = 43.1 bits (100), Expect = 3e-04
 Identities = 31/88 (35%), Positives = 45/88 (51%), Gaps = 1/88 (1%)
 Frame = -2

Query: 95    EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDP-TGVSKGYGFVKFTDELEQKRA 153
             +Y ++  +L  +V D ML  FF + YPS     V+ D  T  SKG+GF+ F D  +   A
Sbjct: 12394 DYRIYANNLGNEVTDQMLTTFF-QSYPSFSKACVIRDKRTNRSKGFGFISFLDSNDYIHA 12218

Query: 154   LTECQGAVGLGCKPVRLSVAIPKASRVK 181
             L    G   LG +P++L  +  K   VK
Sbjct: 12217 LENLNGKY-LGNRPLQLKPSKWKDRNVK 12137


>gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_35, whole genome shotgun
              sequence
          Length = 384281

 Score = 42.0 bits (97), Expect = 7e-04
 Identities = 24/49 (48%), Positives = 30/49 (61%), Gaps = 1/49 (2%)
 Frame = -3

Query: 97     SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDP-TGVSKGYGFVKF 144
              +LF+  L  DVDD  L E F K +      KV+ DP T +SKGYGFVK+
Sbjct: 302046 NLFIFHLPGDVDDSKLMELFSK-FGEIESVKVIRDPKTNLSKGYGFVKY 301903


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.317    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,672,090
Number of Sequences: 784
Number of extensions: 107040
Number of successful extensions: 388
Number of sequences better than 1.0e-03: 5
Number of HSP's better than  0.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 319
Number of HSP's gapped (non-prelim): 120
length of query: 287
length of database: 13,654,695
effective HSP length: 101
effective length of query: 186
effective length of database: 13,575,511
effective search space: 2525045046
effective search space used: 2525045046
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 95 (41.2 bits)
