TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|147898771|ref|NP_001085619.1| eukaryotic elongation factor,
selenocysteine-tRNA-specific [Xenopus laevis]
         (575 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743898.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   153   3e-77
gb|GG743902.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    62   2e-09
gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    59   2e-08
gb|GG743933.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    47   5e-05
gb|GG743889.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    45   3e-04
gb|GG743937.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    43   0.001

>gb|GG743898.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.16, whole
              genome shotgun sequence
          Length = 619246

 Score =  153 bits (387), Expect(4) = 3e-77
 Identities = 88/187 (47%), Positives = 118/187 (63%), Gaps = 2/187 (1%)
 Frame = -1

Query: 48     GITLDLGFSSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDV 107
              GITLDLGFSS  +P     +G  Y   Q TLVDCPGHASLI+T+IGGA IID+ +LVID 
Sbjct: 246268 GITLDLGFSSLQLPFTA-ASGEAYTA-QLTLVDCPGHASLIKTVIGGAHIIDMALLVIDA 246095

Query: 108    TKGMQTQSAECLVIGQIACNKMVVVLNKIDLLAENKRQAAIDKMTKKMQKTLEST-KFHG 166
               KG+Q Q+ E  V+ +IA + ++VVLNK+DLL +   Q  +  M   ++  L S+     
Sbjct: 246094 VKGIQMQTIESTVLAEIATSHVIVVLNKVDLLTDKATQ--LPAMLATIRTFLSSSPTLQD 245921

Query: 167    SPIVSVAA-KPGGPEAPESVTGLGISDLIELLKSHTYLPQRDPHGPFLMAVDHCFSIKGQ 225
               PIV VAA    G  AP+     G+ DL+  ++ H +LP R   GP   A+DHCF ++G+
Sbjct: 245920 CPIVPVAAGDADGARAPQ-----GMPDLLATMQRHLHLPTRSGDGPLCYAIDHCFPLRGK 245756

Query: 226    GTVMTGT 232
              GTV+TGT
Sbjct: 245755 GTVLTGT 245735



 Score =  120 bits (302), Expect(4) = 3e-77
 Identities = 76/217 (35%), Positives = 111/217 (51%), Gaps = 10/217 (4%)
 Frame = -2

Query: 233    ILSGSISLNDNVEIPALKVTKKVKSMQMFHKPVSRAMQGDRLGICVTQFDPKLLERGLVC 292
              +LSG++ +ND + IP L   KKVKS+QMFH  V +A+QGDR+G+ +   D   LERGL  
Sbjct: 245691 VLSGTVRVNDTIAIPILGTEKKVKSLQMFHTSVEKAIQGDRVGLRLHGLDAATLERGLAI 245512

Query: 293    TPESLHTIHAAVISVDKIPYFKGSLQTKAKFHITVSHETVMAKVTFFSLPPASFNEE--- 349
              TP+SL  +   V+ V ++ +F    ++ AK H+TV H TV+AK TFF    ASF  E   
Sbjct: 245511 TPKSLSFVLNLVLQVHRVRFFGLDCESGAKVHVTVGHSTVLAKATFFHGSHASFAVENTE 245332

Query: 350    ------LPANAFDFGKEYLYQDGYISKDSKNSEQDKVDNHQGEEAIPKQQWALLEFEKPV 403
                    LPA+A D                                     + LL+ E+ +
Sbjct: 245331 YRYLATLPASATD-----------------------------------HLFVLLQLEREI 245257

Query: 404    TCPKLCLVIGSKLDTDI-HSNTCRLAFHGVLLEGVED 439
                P    VI S+LDT +  SN CR+AF+G ++  +++
Sbjct: 245256 LVPPFSHVICSRLDTTVSQSNVCRIAFYGPVVATLDN 245146



 Score = 52.0 bits (123), Expect(4) = 3e-77
 Identities = 26/73 (35%), Positives = 44/73 (60%), Gaps = 1/73 (1%)
 Frame = -1

Query: 447    LPNLRVFKLKHKQGQVERVNDDYSIIGRSLFKKETNIQLFVGLKVKLSTGED-GLIEGSF 505
              L  L + K+K + G VE+  +    I + LF+KETN+ +F GL V+       G I+G+F
Sbjct: 245092 LSKLHIGKIKERHGLVEKCGEHGVAIVKDLFRKETNLDIFKGLTVQNERSRALGAIDGAF 244913

Query: 506    GQSGKFKIRIPNG 518
              G++GK +++  +G
Sbjct: 244912 GKTGKCRVQFTDG 244874



 Score = 25.0 bits (53), Expect(4) = 3e-77
 Identities = 12/32 (37%), Positives = 14/32 (43%)
 Frame = -2

Query: 17     HIDSGXXXXXXXXXXXXXXXXFDKNPQSKERG 48
              ++DSG                 DKNPQSK RG
Sbjct: 246411 YVDSGKTSLVRSLSTHLSTAALDKNPQSKARG 246316


>gb|GG743902.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.20, whole
              genome shotgun sequence
          Length = 492046

 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 72/326 (22%), Positives = 132/326 (40%), Gaps = 51/326 (15%)
 Frame = +3

Query: 11     NVGVLGHIDSGXXXXXXXXXXXXXXXXFDKNPQSKERGITLDLGFSSFSV------PVPE 64
              N+G +GH+  G                  KN    ER IT+ LG+++  +      P   
Sbjct: 263001 NIGTIGHVAHGKSTVVKAISGVQTVRF--KN--ELERNITIKLGYANAKIYRSEPAPAKA 263168

Query: 65     HLAGTGYQRLQF----------------------TLVDCPGHASLIRTIIGGAQIIDLMM 102
                A TG     F                      + VDCPGH  L+ T++ GA ++D  +
Sbjct: 263169 DEAPTGPMYSAFGSSKPDRFTDEKGVTWALQRHVSFVDCPGHDILMATMLNGAAVMDAAL 263348

Query: 103    LVIDVTKGM-QTQSAECLVIGQIACNKMVVVL-NKIDLLAENKRQAAIDKMTKKMQKTLE 160
              L++   +   Q Q++E L   +I   + +++L NK+DL+  +  +A  +    ++++ + 
Sbjct: 263349 LLVAGNEPCPQPQTSEHLAAVEIMRLQNIIILQNKVDLIKPDAAKAQYE----QVKQFVA 263516

Query: 161    STKFHGSPIVSVAAKPGGPEAPESVTGLGISDLIELLKSHTYLPQRDPHGPFLMAVDHCF 220
               T  H +PI+ ++A          V    I  + E +  +  +P+RD      + V   F
Sbjct: 263517 GTVAHDAPIIPISA----------VLKYNIDVVCEYIVRNIPIPRRDFVSKPKLIVIRSF 263666

Query: 221    SIKG--------QGTVMTGTILSGSISLNDNVE-------------IPALKVTKKVKSMQ 259
               +          QG V  G+IL G + + D +E             +    +  ++ S++
Sbjct: 263667 DVNKPGQDVENLQGGVAGGSILQGVLRIGDEIEVRPGIYSKDEDGKVQCTPIFSRIVSLK 263846

Query: 260    MFHKPVSRAMQGDRLGICVTQFDPKL 285
                H  +  A+ G  +G+  T  DP L
Sbjct: 263847 AEHNELQYAVPGGLIGV-GTLIDPTL 263921


>gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.50, whole
             genome shotgun sequence
          Length = 253698

 Score = 58.5 bits (140), Expect = 2e-08
 Identities = 42/132 (31%), Positives = 69/132 (52%), Gaps = 10/132 (7%)
 Frame = +1

Query: 188   LGISDLIELLKS-HTYLPQ--RDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNV 244
             LG   ++EL+     Y+P   RD   PFL+ V+  FSI G+GTV++G + +G I+  D V
Sbjct: 33652 LGRDAVLELMNQVDEYIPDPTRDFEKPFLLPVEDVFSISGRGTVVSGRVETGVINTGDEV 33831

Query: 245   EIPALK---VTKKVKSMQMFHKPVSRAMQGDRLGICVTQFDPKLLERG-LVCTPESL--H 298
             E+  ++          ++MF K + R   GD +G+ +       + RG ++C P S+  H
Sbjct: 33832 ELVGIRDVPTKTTCTGVEMFRKSLDRGQAGDNVGVLLRGLKRDDVLRGQVLCKPGSISPH 34011

Query: 299   T-IHAAVISVDK 309
             T + A V  + K
Sbjct: 34012 TKVEAGVYVLKK 34047



 Score = 57.0 bits (136), Expect = 6e-08
 Identities = 37/138 (26%), Positives = 59/138 (42%), Gaps = 10/138 (7%)
 Frame = +2

Query: 9     NFNVGVLGHIDSGXXXXXXXXXXXXXXXX---------FDKNPQSKERGITLDLGFSSFS 59
             + NVG +GH+D G                          DK P+ + RGIT+      + 
Sbjct: 33074 HLNVGTIGHVDHGKTTLTAAITRVLSEQGGGKFIAFEDIDKAPEERARGITISTSHVEYE 33253

Query: 60    VPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL 119
                          +  +  VDCPGHA  ++ +I GA  +D  +LV+  T G   Q+ E +
Sbjct: 33254 T-----------DKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSATDGPMPQTREHI 33400

Query: 120   VIG-QIACNKMVVVLNKI 136
             ++  Q+    +VV LNK+
Sbjct: 33401 LLARQVGVPALVVFLNKV 33454


>gb|GG743933.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.51, whole
              genome shotgun sequence
          Length = 226637

 Score = 47.4 bits (111), Expect = 5e-05
 Identities = 33/131 (25%), Positives = 57/131 (43%), Gaps = 7/131 (5%)
 Frame = +2

Query: 14     VLGHIDSGXXXXXXXXXXXXXXXXFDKNPQSKERGITLDLGFSSFSVPVPEH-------L 66
              ++GH+D+G                  K   ++  GIT  +G + F V   +        +
Sbjct: 143015 IMGHVDTGKTKLLDNIRKT-------KVQDAEAGGITQQIGATFFPVEAIKQKTAALQKV 143173

Query: 67     AGTGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIAC 126
                  Y+     ++D PGH S       G+ + D+ +LV+D+  G++ Q+ E L + +   
Sbjct: 143174 TNLQYKLPGLLVIDTPGHESFTNLRSRGSSLCDIAILVVDIMHGLEPQTLESLRLLRAKR 143353

Query: 127    NKMVVVLNKID 137
                 VV LNKID
Sbjct: 143354 APFVVALNKID 143386


>gb|GG743889.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.7, whole
              genome shotgun sequence
          Length = 943373

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 32/125 (25%), Positives = 60/125 (48%), Gaps = 9/125 (7%)
 Frame = -1

Query: 47     RGITLDLGFSSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVID 106
              RG+T+D+G S F     +H+          TL+D PGH   I  +I GA   D+ +LV+ 
Sbjct: 729281 RGVTMDVGTSYFQTST-KHV----------TLLDAPGHRDFIPKMIAGASQADVAVLVVP 729135

Query: 107    VTKGM---------QTQSAECLVIGQIACNKMVVVLNKIDLLAENKRQAAIDKMTKKMQK 157
               + G          QT+    L++  +   +++V +NK+D +   +R+  + + +   + 
Sbjct: 729134 ASTGEFEGAFMKDGQTKE-HTLLVRSLGVTQLIVAVNKMDSVRSPRRRLLMLRRSTGRKT 728958

Query: 158    TLEST 162
               L S+
Sbjct: 728957 ALTSS 728943


>gb|GG743937.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.55, whole
              genome shotgun sequence
          Length = 220287

 Score = 43.1 bits (100), Expect = 0.001
 Identities = 25/90 (27%), Positives = 43/90 (47%)
 Frame = +1

Query: 204    PQRDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNVEIPALKVTKKVKSMQMFHK 263
              P+R       + +   + I G GTV  G + +G +     V+   + +  +VKS++M H+
Sbjct: 143068 PKRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVQFGPVGLQTEVKSVEMHHE 143247

Query: 264    PVSRAMQGDRLGICVTQFDPKLLERGLVCT 293
               +  A+ GD +G  V     K L RG V +
Sbjct: 143248 SLPEAVPGDNVGFNVKNVSVKELRRGYVAS 143337


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.318    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,934,998
Number of Sequences: 1445
Number of extensions: 410823
Number of successful extensions: 1932
Number of sequences better than 1.0e-03: 6
Number of HSP's better than  0.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 54
Number of HSP's that attempted gapping in prelim test: 1542
Number of HSP's gapped (non-prelim): 753
length of query: 575
length of database: 17,710,878
effective HSP length: 109
effective length of query: 466
effective length of database: 17,553,373
effective search space: 8179871818
effective search space used: 8179871818
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 100 (43.1 bits)
