TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|147898771|ref|NP_001085619.1| eukaryotic elongation factor,
selenocysteine-tRNA-specific [Xenopus laevis]
         (575 letters)

Database: P.tricornutum/genome.fa 
           31 sequences; 23,733,684 total letters

Searching...............................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...   397   e-111
gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    91   1e-18
gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    77   3e-14
gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    61   2e-09
gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    52   7e-07
gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    51   2e-06
gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    43   4e-04

>gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun
              sequence
          Length = 2535400

 Score =  397 bits (1020), Expect = e-111
 Identities = 241/629 (38%), Positives = 350/629 (55%), Gaps = 61/629 (9%)
 Frame = +2

Query: 8      LNFNVGVLGHIDSGXXXXXXXXXXXXXXXXFDKNPQSKERGITLDLGFSSFSVPVPEHLA 67
              LN N+GVLGH+DSG                 DK+ QS++RG+TLDLGFS F + +P HL 
Sbjct: 950720 LNVNLGVLGHVDSGKTSLVKALSTLLSTAALDKSKQSRQRGMTLDLGFSCFFLDMPSHLQ 950899

Query: 68     GT--GYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIA 125
               +    + LQ TLVDCPGHASLIRTIIGGAQIID+++LV+D  KG Q Q+ ECLV+ ++ 
Sbjct: 950900 ASYPDKKNLQITLVDCPGHASLIRTIIGGAQIIDMVLLVVDAVKGWQAQTTECLVLAELT 951079

Query: 126    CNKMVVVLNKIDLLAENKRQAAIDKMTKKMQKTLESTKFHGSPIVSVAAKPGGPE---AP 182
                 +VV LNK D     +R   +++   K++  L++T+F  +PIV VAA  GG +   A 
Sbjct: 951080 SPYLVVALNKADQFPPPERDQLLEQAAAKIRDRLKATRFANAPIVGVAACVGGEKVAAAS 951259

Query: 183    ESVTGLGISD-------LIELLKSHTYLPQRDPHGPFLMAVDHCFSIKGQGTVMTGTILS 235
              E ++ L + +       L++ L+     P+R P G F  +VDHCFSI+G+GTV+TGT+L+
Sbjct: 951260 EDMSKLEVRNETYNMETLVQTLQDKLPPPKRTPEGSFYFSVDHCFSIRGRGTVLTGTVLN 951439

Query: 236    GSISLNDNVEIPALKVTKKVKSMQMFHKPVSRAMQGDRLGICVTQFDPKLLERGLVCTPE 295
              G+ S+ND +E P L + +K+KS+QMF + V R  QGDR G+CV+  D KLLERG+  TP 
Sbjct: 951440 GAASVNDVLEFPTLGLDRKIKSIQMFKRQVQRIQQGDRAGVCVSNLDAKLLERGIAATPG 951619

Query: 296    SLHTIHAAVISVDKIPYFKGSLQTKAKFHITVSHETVMAKVTFFSLPPASFNEELPAN-- 353
              ++  +  A+  V K+PY+ G+L + +KFHI+V H T+MA VTF+        E+ PA+  
Sbjct: 951620 AVQLLKGAIALVRKVPYYVGTLHSGSKFHISVGHTTIMATVTFWGAVELLGKEQNPADLS 951799

Query: 354    -------------------------------AFDFGKEYLYQDGYISKDSKNSEQDKVDN 382
                                              F F +E+++QD            D +++
Sbjct: 951800 VVNPVDTKPSKRYVGSSSLGGDADMAGLPRMTFSFEEEFIHQD------------DLLES 951943

Query: 383    HQGEEAIPKQQWALLEFEKPVTCPKLCLVIGSKLDTDIHS----NTCRLAFHGVLLEGVE 438
                G+E++    WALL+F+ PV CP   LVIGS+LDT  +S    + CRLAF G L++ V+
Sbjct: 951944 LDGQESL--LHWALLDFQIPVHCPMDSLVIGSRLDTVDNSTGSASACRLAFSGRLMQSVD 952117

Query: 439    -DKNYSESFLPNLRVFKLKHKQGQVERVNDD-----------YSIIGRSLFKKETNIQLF 486
                K+ S+     LR++  K ++G + R+ D            Y + G  LFKKETN++ F
Sbjct: 952118 PGKDISK-----LRLYTPKERRGTISRLGDPHKRTDDDKLVRYEVFGSDLFKKETNMKAF 952282

Query: 487    VGLKVKLSTGEDGLIEGSFGQSGKFKIRIPNGLQLETKKMLVTSNKKRKGKGDVEAAKEE 546
               G+KV+    E G I+ SFG SGK+++  P G +                      AKE 
Sbjct: 952283 FGMKVQTERNEIGEIKSSFGTSGKYRVFFPAGTE----------------------AKEG 952396

Query: 547    EIKQDSQPILITLWFKRYMFDPQKKMIQS 575
              E      P++  L FKR+  DP K M Q+
Sbjct: 952397 E------PLI--LRFKRFAHDPTKAMHQT 952459



 Score = 45.4 bits (106), Expect = 9e-05
 Identities = 40/154 (25%), Positives = 64/154 (41%), Gaps = 14/154 (9%)
 Frame = +1

Query: 56     SSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQS 115
              S+F VP+ +H  G        T +D PGHA+       G+   D+++LVI    G+  Q+
Sbjct: 742351 SAFQVPLLDHAVGA------VTFLDTPGHAAFKTMRQSGSDAADILVLVIAADDGVSEQT 742512

Query: 116    AECLVIGQIACN-------KMVVVLNKIDLLAENKRQAAIDKMTKKMQKTLESTKFHGSP 168
               E L   +            +V+ +NKID     K    +D+   +++  L     HG  
Sbjct: 742513 VEILDFYKSIITSSGGSGISLVIAMNKID-----KPGIEVDEARMRIESQLLE---HGIL 742668

Query: 169    IVSVAAKPGGPEAP-------ESVTGLGISDLIE 195
                 + +   G   P         +TG G+ DLIE
Sbjct: 742669 TEGMPSDQDGEYGPPVQLVPVSGLTGQGLDDLIE 742770


>gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 21, whole genome shotgun
              sequence
          Length = 662217

 Score = 91.3 bits (225), Expect = 1e-18
 Identities = 62/224 (27%), Positives = 113/224 (50%), Gaps = 6/224 (2%)
 Frame = +3

Query: 82     PGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIG-QIACNKMVVVLNKIDLLA 140
              PGHA  ++ +I GA  +D  +LV+  T G   Q+ E +++  Q+    +VV LNK+DL+ 
Sbjct: 404019 PGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLAKQVGIPNLVVFLNKVDLVD 404198

Query: 141    ENKRQAAIDKMTKKMQKTLESTKFHGS--PIVSVAAKPGGPEAPESVTGLGISDLIELLK 198
              + +    ++ +  ++++ L+   F G   PI+  +A          +    I +L+  + 
Sbjct: 404199 DEE---LLELVEMEIRELLDFYDFPGDDIPIIRGSALAAAEGRNPEIGSEKILELMAAVD 404369

Query: 199    SHTYLPQRDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNVEIPALKVTKKV--K 256
                   P RD    FLM ++  FSI G+GTV+TG +  G +++ D +++  L    K    
Sbjct: 404370 EKIPEPMRDLDKDFLMPIEDVFSIAGRGTVVTGRVQQGKVNVGDELDVIGLDQNHKTICT 404549

Query: 257    SMQMFHKPVSRAMQGDRLGICVTQFDPKLLERG-LVCTPESLHT 299
               ++MF K +   M GD +G  +     + + RG ++C P S+ T
Sbjct: 404550 GVEMFKKLLDFGMAGDNIGALLRGLKREDVRRGQILCKPGSMKT 404681


>gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 13, whole genome shotgun
              sequence
          Length = 887524

 Score = 77.0 bits (188), Expect = 3e-14
 Identities = 66/268 (24%), Positives = 113/268 (42%), Gaps = 13/268 (4%)
 Frame = +3

Query: 39     DKNPQSKERGITLDLGFSSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQII 98
              D     +ERGIT+D+    F  P           +  FT++D PGH   I+ +I G    
Sbjct: 601719 DNLKAERERGITIDIALWKFESP-----------KYSFTVIDAPGHRDFIKNMITGTSQA 601865

Query: 99     DLMMLVID---------VTKGMQTQSAECLVIGQIACNKMVVVLNKIDLLAENKRQAAID 149
              D+ +LVID         ++K  QT+    L+   +   +M+V +NK+D       +    
Sbjct: 601866 DVAVLVIDSSQGGFEAGISKDGQTRE-HALLAYTLGVKQMIVAMNKMDDKTVKYAEDRYT 602042

Query: 150    KMTKKMQKTLESTKFHGS--PIVSVAAKPGGPEAPESVTGLGISD--LIELLKSHTYLPQ 205
              ++  ++   L+   +     P V ++   G     +S          L+E L S T  P+
Sbjct: 602043 EIKNEVSAYLKKVGYKPMKIPFVPISGWEGDNMVEKSTNMAWYKGPYLLEALDSVTP-PK 602219

Query: 206    RDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNVEIPALKVTKKVKSMQMFHKPV 265
              R       + +   + I G GTV  G + +G I    +       +  ++KS++M H+ +
Sbjct: 602220 RPTDKALRLPLQDVYKIGGIGTVPVGRVETGVIKPGIHAMFAPSGIIAEIKSVEMHHESL 602399

Query: 266    SRAMQGDRLGICVTQFDPKLLERGLVCT 293
                A+ GD +G  V     K L RG V +
Sbjct: 602400 PEAVPGDNVGFNVKNVAVKDLRRGFVAS 602483


>gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 23, whole genome
             shotgun sequence
          Length = 512847

 Score = 60.8 bits (146), Expect = 2e-09
 Identities = 63/239 (26%), Positives = 109/239 (45%), Gaps = 22/239 (9%)
 Frame = +2

Query: 69    TGYQRLQFTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACN 127
             T + R   + VDCPGH  L+ T++ GA ++D  +L+I   +   Q Q++E L   +I   
Sbjct: 45884 TYHLRRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNETCPQPQTSEHLAAVEIMRL 46063

Query: 128   KMVVVL-NKIDLLAENKRQAAIDKMTKKMQKTLESTKFHGSPIVSVAAKPGGPEAPESVT 186
             + +++L NK+DL+   K  AA+ +  ++++K +  T    +PI+ ++A          V 
Sbjct: 46064 EHILILQNKVDLV---KPDAAVAQQ-EQIRKFVAGTVADAAPILPISA----------VL 46201

Query: 187   GLGISDLIELLKSHTYLPQRDPHGPFLMAVDHCFSI--------KGQGTVMTGTILSGSI 238
                +  L E L     LP RD      + V   F +        K QG V  G+IL G +
Sbjct: 46202 RYNMDVLCEYLIRRIPLPVRDFTSQPRLIVIRSFDVNRPGQDVSKLQGGVAGGSILQGVL 46381

Query: 239   SLNDNVE------------IPALKVTKKVKSMQMFHKPVSRAMQGDRLGICVTQFDPKL 285
              + D +E            I  + +  K+ S+      +  A+ G  +G+  T+ DP L
Sbjct: 46382 RVGDEIEVRPGIVHKQDDKIVCVPIFSKISSLYAEQNDLQFAVPGGLIGV-GTKIDPTL 46555


>gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun
              sequence
          Length = 1098047

 Score = 52.4 bits (124), Expect = 7e-07
 Identities = 67/332 (20%), Positives = 127/332 (38%), Gaps = 34/332 (10%)
 Frame = -1

Query: 39     DKNPQSKERGITLDLGFSSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQII 98
              D N + + +G T+++G + F   +            ++T++D PGH + +  +I GA   
Sbjct: 710765 DTNEEERAKGKTVEVGKAQFETDLK-----------RYTILDAPGHKNYVPNMIQGASQA 710619

Query: 99     DLMMLVIDVTKGM---------QTQSAECLVIGQIACNKMVVVLNKIDLLAENKRQAAID 149
              D+ +LVI   KG          QT+    ++   +  + +VV +NK+D      ++   D
Sbjct: 710618 DVGVLVISARKGEFETGFDRGGQTRE-HAMLAKTLGVSYLVVAVNKMDEPTVQWKKERFD 710442

Query: 150    KMTKKMQKTLESTKF---HGSPIVSVAAKPGGPEAPE---------------SVTGLGIS 191
              +   K++  L+S  F        + ++   G     E               +       
Sbjct: 710441 ECVTKIRPFLKSCGFVIKREVKFIPISGLSGANVKDEVALSECSWWKEYYTSNENNTSAP 710262

Query: 192    DLIELLKSHTYLPQRDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNVEIPALKV 251
               L+ LL +   +  RDP  P  + V   ++   +G +  G + SG I     V I   + 
Sbjct: 710261 TLLSLLDT-LEIAGRDPEAPLRIPVLDRYT--DRGIISMGKVESGIIRPGMKVTIMPTRN 710091

Query: 252    TKKVKSMQMFHKPVSRAMQGDRLGICVTQFDPKLLERG-LVCTPESLHTIHAAVISV--- 307
                KV  +     PVS A  G+ + + +     + + +G ++CT      +   +  +   
Sbjct: 710090 KYKVDEVWANEDPVSGARPGENVLVKLNGAKMEDVRKGFVICTDPPCRAVDKIICQIAVA 709911

Query: 308    ---DKIPYFKGSLQTKAKFHITVSHETVMAKV 336
                 D  P      Q     H T   E  +AK+
Sbjct: 709910 DMPDNTPIMTAGFQCMFHAH-TTEEECTVAKI 709818


>gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 18, whole genome shotgun
              sequence
          Length = 702471

 Score = 50.8 bits (120), Expect = 2e-06
 Identities = 41/172 (23%), Positives = 75/172 (43%), Gaps = 1/172 (0%)
 Frame = -3

Query: 78     LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACNKMVVVLNKID 137
              L+D PGH      +       +  +LV+D ++G++ Q+   + +      +++ +LNKID
Sbjct: 516367 LIDTPGHVDFSYEVSRSLAACEGALLVVDASQGIEAQTLANVYLALENDLEIIPILNKID 516188

Query: 138    LLAENKRQAAIDKMTKKMQKTLEST-KFHGSPIVSVAAKPGGPEAPESVTGLGISDLIEL 196
              L A +  + A         + +E+T     S IV  +AK          TG+GI D++E 
Sbjct: 516187 LPAADPERVA---------EEIEATIGLDCSGIVHASAK----------TGIGIDDILER 516065

Query: 197    LKSHTYLPQRDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDNVEIPA 248
              +      P     GPF   +   +    +G ++   ++ G +S  D V   A
Sbjct: 516064 IVQMVPPPPAATGGPFRALIFDSYYDAYRGVIVFFRVMDGEVSQGDKVRFLA 515909



 Score = 46.2 bits (108), Expect = 5e-05
 Identities = 55/263 (20%), Positives = 99/263 (37%), Gaps = 28/263 (10%)
 Frame = +1

Query: 39     DKNPQSKERGITLDLGFSSFSVPVPEHLAGTGYQRLQFTLVDCPGHASLIRTIIGGAQII 98
              D++ Q +  G+T+D+   +               R    L D PGHA  +  +I G    
Sbjct: 619114 DEDDQERAHGVTMDIATKTIETA-----------RYNVVLQDAPGHADYVPAMITGTASA 619260

Query: 99     DLMMLVIDVTKGMQTQSAE-------CLVIGQIACNKMVVVLNKIDLLAENKRQAAIDKM 151
              D  +LV+D T G    + +         V   +  N+++VV+NK+DL+  +  Q   + M
Sbjct: 619261 DAALLVVDATDGAAHTALQSGQLKEHAYVARGLGVNQILVVINKMDLV--DWSQEVYESM 619434

Query: 152    TKKMQKTLESTKFHGSPI--VSVAAKPG-----------GPEAPESVTGLGI-----SDL 193
                 ++  L S  +  S +  V V+   G            P        L         L
Sbjct: 619435 RFTLETFLTSIGYAESKLRYVPVSGLTGVNVYTDKRKRVDPTTAAEYAALSSWYENGPTL 619614

Query: 194    IELLKSHTYLPQRDPHGPFLMAVDHCFSIKGQGTVMTGTILSGSISLNDN---VEIPALK 250
               + L       +++   P L+ V      +G+G  +   + +G + LN+    + I    
Sbjct: 619615 WQALDGFVAPVRKNVEKPLLVIVTDVLGEQGKGVAVRAKMATGWLGLNETLIVLPIGDAV 619794

Query: 251    VTKKVKSMQMFHKPVSRAMQGDR 273
                 K+ S+Q      S++   DR
Sbjct: 619795 TVHKLASVQSLEASGSQSSATDR 619863


>gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 27, whole genome shotgun
              sequence
          Length = 404298

 Score = 43.1 bits (100), Expect = 4e-04
 Identities = 37/134 (27%), Positives = 58/134 (43%), Gaps = 8/134 (5%)
 Frame = -2

Query: 12     VGVLGHIDSGXXXXXXXXXXXXXXXXFDKNPQSKERG-ITLDLGFSSFSVPVPEH----L 66
              V ++GH+D+G                   N Q  E G IT  +G + F     E     L
Sbjct: 276773 VVIMGHVDTGKTKLLDKIRKT--------NVQEGEAGGITQQIGATYFEKKTLEQQVSKL 276618

Query: 67     AGTGYQRLQ---FTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQ 123
                T    L+     ++D PGH S       G+ + D+ +LV+D+  G++ Q+ E L + +
Sbjct: 276617 NATENVELKVPGMLVIDTPGHESFTNLRSRGSSLCDVAILVVDLMHGLEQQTIESLTMLR 276438

Query: 124    IACNKMVVVLNKID 137
                    VV LNK+D
Sbjct: 276437 KRGVPFVVALNKVD 276396


  Database: P.tricornutum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 23,733,684
  Number of sequences in database:  31
  
Lambda     K      H
   0.318    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,959,839
Number of Sequences: 31
Number of extensions: 197482
Number of successful extensions: 943
Number of sequences better than 1.0e-03: 7
Number of HSP's better than  0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 802
Number of HSP's gapped (non-prelim): 296
length of query: 575
length of database: 7,911,228
effective HSP length: 104
effective length of query: 471
effective length of database: 7,908,004
effective search space: 3724669884
effective search space used: 3724669884
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 97 (42.0 bits)
