TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000092_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Drosophila melanogaster # Complete
         (511 letters)

Database: E.siliculosus/genome.fa 
           35 sequences; 195,934,119 total letters

Searching...................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genom...    85   7e-16
emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genom...    77   2e-13
emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genom...    47   2e-04
emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genom...    46   4e-04

>emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly,
                unassigned sequences LGUn
          Length = 58155991

 Score = 85.1 bits (209), Expect = 7e-16
 Identities = 42/61 (68%), Positives = 50/61 (81%), Gaps = 3/61 (4%)
 Frame = +1

Query: 72       VDCPGHASLIRTIIGGAQIIDLMLLVVDAQKGKQTQTAECLIIGELLQKK---LIVVINK 128
                VDCPGHASLIRTIIGGAQI+D+MLLVVDA KG Q QT EC++IGE +      +IVV+NK
Sbjct: 17111767 VDCPGHASLIRTIIGGAQIMDMMLLVVDATKGIQAQTTECVVIGEAVMSSGADVIVVLNK 17111946

Query: 129      I 129
                +
Sbjct: 17111947 V 17111949



 Score = 58.5 bits (140), Expect = 7e-08
 Identities = 25/36 (69%), Positives = 32/36 (88%)
 Frame = +2

Query: 3        INFNIGLLGHVDSGKTTLAKALSSISSTAAFDKNPQ 38
                +N N+G+LGHVDSGKT+L KALS++ STA+ DKNPQ
Sbjct: 17110598 LNINVGILGHVDSGKTSLVKALSTVLSTASLDKNPQ 17110705



 Score = 48.9 bits (115), Expect = 5e-05
 Identities = 19/37 (51%), Positives = 27/37 (72%)
 Frame = +2

Query: 188      PQRNLADPLFMYVDHCFGIKGQGTVCTGTLLQGKVQV 224
                P R  + PL+   DHCF ++GQGT+ TGT+L G+V+V
Sbjct: 17112845 PSRRASAPLYFAFDHCFSVRGQGTILTGTVLTGEVKV 17112955



 Score = 47.4 bits (111), Expect = 2e-04
 Identities = 23/56 (41%), Positives = 37/56 (66%), Gaps = 7/56 (12%)
 Frame = +1

Query: 255      GDRIGLCVTQFNAKLLERGIITQPGYLKPIYA-------VCLQFKPIRYYKEVIKS 303
                GDR+G+CV   +AKL+ERG++T PG ++PI A       VC +F+  R ++  ++S
Sbjct: 17113384 GDRVGMCVASLDAKLMERGVVTTPGSVRPISAAIALVRKVC-RFETWRSHRSTLQS 17113548



 Score = 45.4 bits (106), Expect = 6e-04
 Identities = 26/51 (50%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
 Frame = +2

Query: 24       LSSISSTAAFDKNPQSVERGITLDLGFSGLLVDAPAHLPQGE--QLQFTFV 72
                LS     + +    QS ERGITLDLGFS   V  P HLPQ +   LQFT V
Sbjct: 17111159 LSPRGPPSRYTVRTQSRERGITLDLGFSAFTVSLPEHLPQDQYSHLQFTLV 17111311


>emb|FN649736.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG11
          Length = 5521395

 Score = 77.0 bits (188), Expect = 2e-13
 Identities = 81/321 (25%), Positives = 129/321 (40%), Gaps = 48/321 (14%)
 Frame = +1

Query: 3       INFNIGLLGHVDSGKTTLAKAL------------------------SSISSTAAFDKNPQ 38
               I+ N+ ++GHVD+GK+T    L                        SS       D    
Sbjct: 2322619 IHINLVVIGHVDAGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKSSFKYAWVLDNLKA 2322798

Query: 39      SVERGITLDLGFSGLLVDAPAHLPQGEQLQFTFVDCPGHASLIRTIIGGAQIIDLMLLVV 98
                 ERGIT+D+              +  +  FT +D PGH   I+ +I G    D+ +LVV
Sbjct: 2322799 ERERGITIDIALWKF---------ESPKYNFTVIDAPGHRDFIKNMITGTSQADVAVLVV 2322951

Query: 99      DAQKG-------KQTQTAE-CLIIGELLQKKLIVVINKID----VYPENQRASKLEKLRL 146
                +  G       K  QT E  L+   L  K++IV INK+D    +Y E +     E++ +
Sbjct: 2322952 ASGVGEFEAGISKNGQTREHALLAFTLGVKQMIVCINKMDDSSVMYGEPRYTEIKEEVAI 2323131

Query: 147     RLAKTLEATTFGGQVPICAVSA------------LQGTHIAELREVLREAYFQPQRNLAD 194
                L K          VPI   +              +G ++ E  + ++E    P R    
Sbjct: 2323132 YLKKVGYKPAKIPFVPISGWAGDNMIDKSTNMPWYKGPYLLEALDTMKE----PTRPTDK 2323299

Query: 195     PLFMYVDHCFGIKGQGTVCTGTLLQGKVQVNNVIELPALGEQRKVKSIQMFRKNVTSASM 254
               PL + +   + I G GTV  G +  G ++   V+         +VKS++M  + +  A  
Sbjct: 2323300 PLRLPLQDVYKIGGIGTVPVGRVETGCLKPGMVVTFAPCMLDTEVKSVEMHHEALPEAVP 2323479

Query: 255     GDRIGLCVTQFNAKLLERGII 275
               GD +G  V   + K + RG +
Sbjct: 2323480 GDNVGFNVKNVSVKDIRRGYV 2323542



 Score = 77.0 bits (188), Expect = 2e-13
 Identities = 81/321 (25%), Positives = 129/321 (40%), Gaps = 48/321 (14%)
 Frame = -2

Query: 3       INFNIGLLGHVDSGKTTLAKAL------------------------SSISSTAAFDKNPQ 38
               I+ N+ ++GHVD+GK+T    L                        SS       D    
Sbjct: 2318561 IHINLVVIGHVDAGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKSSFKYAWVLDNLKA 2318382

Query: 39      SVERGITLDLGFSGLLVDAPAHLPQGEQLQFTFVDCPGHASLIRTIIGGAQIIDLMLLVV 98
                 ERGIT+D+              +  +  FT +D PGH   I+ +I G    D+ +LVV
Sbjct: 2318381 ERERGITIDIALWKF---------ESPKYNFTVIDAPGHRDFIKNMITGTSQADVAVLVV 2318229

Query: 99      DAQKG-------KQTQTAE-CLIIGELLQKKLIVVINKID----VYPENQRASKLEKLRL 146
                +  G       K  QT E  L+   L  K++IV INK+D    +Y E +     E++ +
Sbjct: 2318228 ASGVGEFEAGISKNGQTREHALLAFTLGVKQMIVCINKMDDSSVMYGEPRYTEIKEEVAI 2318049

Query: 147     RLAKTLEATTFGGQVPICAVSA------------LQGTHIAELREVLREAYFQPQRNLAD 194
                L K          VPI   +              +G ++ E  + ++E    P R    
Sbjct: 2318048 YLKKVGYKPAKIPFVPISGWAGDNMIDKSTNMPWYKGPYLLEALDTMKE----PTRPTDK 2317881

Query: 195     PLFMYVDHCFGIKGQGTVCTGTLLQGKVQVNNVIELPALGEQRKVKSIQMFRKNVTSASM 254
               PL + +   + I G GTV  G +  G ++   V+         +VKS++M  + +  A  
Sbjct: 2317880 PLRLPLQDVYKIGGIGTVPVGRVETGCLKPGMVVTFAPCMLDTEVKSVEMHHEALPEAVP 2317701

Query: 255     GDRIGLCVTQFNAKLLERGII 275
               GD +G  V   + K + RG +
Sbjct: 2317700 GDNVGFNVKNVSVKDIRRGYV 2317638


>emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG17
          Length = 5807719

 Score = 47.0 bits (110), Expect = 2e-04
 Identities = 23/59 (38%), Positives = 32/59 (54%)
 Frame = +2

Query: 70      TFVDCPGHASLIRTIIGGAQIIDLMLLVVDAQKGKQTQTAECLIIGELLQKKLIVVINK 128
               TFVD PGHA+     + GA   D+++LVV A  G   QT E +    +    ++V INK
Sbjct: 1019297 TFVDTPGHAAFSEMRVRGANATDIVVLVVAADDGVMEQTKESIATARMADVPIVVAINK 1019473


>emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence
               assembly, chromosome LG02
          Length = 9289656

 Score = 46.2 bits (108), Expect = 4e-04
 Identities = 31/104 (29%), Positives = 51/104 (49%)
 Frame = +3

Query: 7       IGLLGHVDSGKTTLAKALSSISSTAAFDKNPQSVERGITLDLGFSGLLVDAPAHLPQGEQ 66
               + ++GHVD GKTTL  AL +  +  A      +   GIT  +G         A   + + 
Sbjct: 8437794 VSVMGHVDHGKTTLLDALRTSKAKVA-----GTEAAGITQKIG---------AFSVEVKD 8437931

Query: 67      LQFTFVDCPGHASLIRTIIGGAQIIDLMLLVVDAQKGKQTQTAE 110
                  TF D PGHA+        +++ D++++V+ A  G ++QT E
Sbjct: 8437932 SSVTFFDTPGHAAFKSMRHATSRLTDIVVVVIAADDGVKSQTVE 8438063


  Database: E.siliculosus/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 195,934,119
  Number of sequences in database:  35
  
Lambda     K      H
   0.320    0.136    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 105,734,280
Number of Sequences: 35
Number of extensions: 1629088
Number of successful extensions: 6827
Number of sequences better than 1.0e-03: 4
Number of HSP's better than  0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 6171
Number of HSP's gapped (non-prelim): 2679
length of query: 511
length of database: 65,311,373
effective HSP length: 117
effective length of query: 394
effective length of database: 65,307,278
effective search space: 25731067532
effective search space used: 25731067532
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 104 (44.7 bits)
