TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SecS D.rerio
         (490 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   369   e-102

>gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun
              sequence
          Length = 941567

 Score =  369 bits (948), Expect = e-102
 Identities = 203/460 (44%), Positives = 293/460 (63%), Gaps = 11/460 (2%)
 Frame = +3

Query: 8      LSEKLVSASYVRQGLDARRGHEQLIRRLLDQGKCPEEGWSESTIELFLNELAVMDSNNFL 67
              ++  L+ A++V+Q L A    +++ + LL Q + P+EG+S+  IE  L+  A MD+NN+ 
Sbjct: 822903 IASTLIQANHVKQALQAVNKQQKMYKTLLSQRQLPKEGFSDQQIESLLSFFASMDTNNYE 823082

Query: 68     GNCGVGEREGRVASSLVARRHYRLIHGIGRSGDIAAVQPKAAGSSLLNKITNSVVLDVLK 127
               + G+GEREGRV S LV +RH+ L HGIGRSG++ + QPKAAGSS++ ++ N +V   ++
Sbjct: 823083 NHIGIGEREGRVFSKLVEKRHFYLSHGIGRSGELTSNQPKAAGSSIILQLCNYLVNHAIQ 823262

Query: 128    LTGVRSVSSCFVVPMATGMSLTLCFLTLRH--RRPKARYILWPRIDQKSCFKSMVTAGFE 185
              L G ++V    VVPM TGM+L LC  +L +   +P  +Y++WPRIDQ+S  KS+ TAGF 
Sbjct: 823263 LAGCKNVKKSIVVPMCTGMTLALCLRSLANSLEQPNKKYVIWPRIDQQSVLKSIDTAGFT 823442

Query: 186    PVVIENVLEGD-ELRTNLEEVERKIEEFGAENTLCVHSTTSCFAPRVPDRLEELSVLCAK 244
              P+VIENVL  D  +  NL+ +E KI++ GAEN LCV STTSCFAPRVPD + E+  +C K
Sbjct: 823443 PIVIENVLNEDGSIEANLQSIESKIQQVGAENILCVLSTTSCFAPRVPDDVFEIGKICFK 823622

Query: 245    HDIPHIVNNAYGVQPSKCMHLIQQ--GARVGRIDAFVQSLDKNFVVPVGGAII-AGFDEN 301
              + I  +VNNAYGVQ    M  I +   +    I  FVQS DKNF+VPVGGA++ +G D  
Sbjct: 823623 YGIGQVVNNAYGVQSKIIMERINKTIASSKSTITCFVQSTDKNFMVPVGGAVVSSGSDIT 823802

Query: 302    FIQEISKIYPGRASASPSLDVLITLLTLGANGYKKLLADRKELYGHLAQELSALAARHGE 361
              FI ++SKIY GRAS +P LD+ ITLL++G +G++KLLA+R+ L+ H   E+ +       
Sbjct: 823803 FIDKVSKIYAGRASMTPILDLFITLLSMGKDGWEKLLAEREVLFQHFVSEIESKLTNTQS 823982

Query: 362    RLL----KTPHNPISLAMSLNHLEAHSSSAVTQLGSMLFTRQVSGARVVPLGVQQTVSGH 417
              +          N IS A+S++           ++G  LF R  +G RVV  G  +TV  H
Sbjct: 823983 KFSLIRGSLQRNQISFALSID----LPKEEALKVGVSLFYRNCTGPRVVLKGSSKTVLPH 824150

Query: 418    -TFSGFMSHSEAYPCPYLNAASAIGITKGDVTVCMKRLGK 456
               +F G+ SH+++YP  Y+  A AIGI K ++   +  L K
Sbjct: 824151 LSFVGYGSHTDSYPHHYITLACAIGIEKEEIESFLTLLNK 824270


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.319    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,999,115
Number of Sequences: 784
Number of extensions: 276158
Number of successful extensions: 1199
Number of sequences better than 1.0e-03: 1
Number of HSP's better than  0.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1063
Number of HSP's gapped (non-prelim): 303
length of query: 490
length of database: 13,654,695
effective HSP length: 106
effective length of query: 384
effective length of database: 13,571,591
effective search space: 5211490944
effective search space used: 5211490944
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 98 (42.4 bits)
