TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DI2 Xenopus laevis
         (262 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738919.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    47   1e-05
gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    28   8.5  

>gb|GG738919.1| Naegleria gruberi genomic scaffold NAEGRscaffold_75, whole genome shotgun
              sequence
          Length = 216243

 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 43/187 (22%), Positives = 80/187 (42%), Gaps = 4/187 (2%)
 Frame = +1

Query: 75     DAYKQVKLGQDAPNSNVIQVSNNRTSKSVQRKFAGKCHLLDFASSERPLVVNFGSATXPP 134
              D  K++      P+S  +  +N +T             L +  ++  P+ +   S + PP
Sbjct: 112690 DFEKEINNFDQMPSSEELVANNTKTLAQF---------LTERNTNNLPVAIIGSSYS*PP 112842

Query: 135    FIS-QLPAFSKLVEEFSSVADFVLVYIDEAHPSDGWAAPGTASYEVKKHRSQEERCAAAS 193
              F   Q   F  + +EF      + +YI EAH  D W      +  +K+H++ E+R  A  
Sbjct: 112843 FRQHQQYLFESIFQEFKEKVFVITIYIKEAHACDQWKLGNRIN--IKQHQTSEDRVDATR 113016

Query: 194    KLLQ--HFSIPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIV-YLGGKGPFFYNIQE 250
               +++  +F IP     + D ++N    A+ V  E+  I+    IV Y      + ++  +
Sbjct: 113017 LMMKEMNFRIP----TIVDSIENGYEEAFAVWPEKFVIMNNNGIVEYQSKPETYGHSPTK 113184

Query: 251    IRRWLEL 257
               R  +EL
Sbjct: 113185 FRELIEL 113205


>gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome
             shotgun sequence
          Length = 536960

 Score = 28.1 bits (61), Expect = 8.5
 Identities = 17/54 (31%), Positives = 25/54 (46%)
 Frame = +2

Query: 24    FSNCLFLALYDSVVLVKHVLLQLNRSKSSQSQWRRMLTPEGLRCVWNSFLLDAY 77
             F  C  + L+  + LV+ V+   NR + S S W R       + + N  LLD Y
Sbjct: 92927 FCCCSIIFLF*FMCLVQEVI---NRKRKSFSNWERFTDSTIPKYIINKLLLDTY 93079


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.322    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,714,656
Number of Sequences: 784
Number of extensions: 180665
Number of successful extensions: 831
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 54
Number of HSP's successfully gapped in prelim test: 26
Number of HSP's that attempted gapping in prelim test: 650
Number of HSP's gapped (non-prelim): 440
length of query: 262
length of database: 13,654,695
effective HSP length: 100
effective length of query: 162
effective length of database: 13,576,295
effective search space: 2199359790
effective search space used: 2199359790
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
