TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: S.parasitica/genome.fa 
           1445 sequences; 53,132,636 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG743898.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...   153   3e-74
gb|GG743902.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    90   9e-18
gb|GG743933.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    62   3e-09
gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    61   4e-09
gb|GG743889.1| Saprolegnia parasitica CBS 223.65 genomic scaffol...    51   4e-06

>gb|GG743898.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.16, whole
              genome shotgun sequence
          Length = 619246

 Score =  153 bits (386), Expect(3) = 3e-74
 Identities = 87/200 (43%), Positives = 122/200 (61%), Gaps = 1/200 (0%)
 Frame = -1

Query: 46     GITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIG 105
              GITLDLGFS   +P  A          A+ EA         Q+TLVDCPGHASLI+T+IG
Sbjct: 246268 GITLDLGFSSLQLPFTA----------ASGEAYTA------QLTLVDCPGHASLIKTVIG 246137

Query: 106    GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTK 165
              GA IID+ +LVID  KG+Q Q+ E  V+ +IA   ++VVLNK+DLL +   Q  +  M  
Sbjct: 246136 GAHIIDMALLVIDAVKGIQMQTIESTVLAEIATSHVIVVLNKVDLLTDKATQ--LPAMLA 245963

Query: 166    KMQKTLENT-KFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPF 224
               ++  L ++   +  PI+PVAA     +A    APQG+P+L+  +   + +PTR   GP 
Sbjct: 245962 TIRTFLSSSPTLQDCPIVPVAA----GDADGARAPQGMPDLLATMQRHLHLPTRSGDGPL 245795

Query: 225    LMSVDHCFSIKGQGTVMTGT 244
                ++DHCF ++G+GTV+TGT
Sbjct: 245794 CYAIDHCFPLRGKGTVLTGT 245735



 Score =  108 bits (270), Expect(3) = 3e-74
 Identities = 70/213 (32%), Positives = 113/213 (53%), Gaps = 1/213 (0%)
 Frame = -2

Query: 245    ILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVC 304
              +LSG++ + D++ IP L   KKVKS+QMFH  +  A+QGDR+G+ +   D   LERGL  
Sbjct: 245691 VLSGTVRVNDTIAIPILGTEKKVKSLQMFHTSVEKAIQGDRVGLRLHGLDAATLERGLAI 245512

Query: 305    APESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPIL 364
               P+SL  V   ++ V ++ +F    ++ AK H+TVGH TV+ +  FF  +  +F  E   
Sbjct: 245511 TPKSLSFVLNLVLQVHRVRFFGLDCESGAKVHVTVGHSTVLAKATFFHGSHASFAVE--- 245341

Query: 365    DSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPR 424
                   + EY    +YL+  L  + TD+                   + L++ E+ +  P 
Sbjct: 245340 -----NTEY----RYLA-TLPASATDH------------------LFVLLQLEREILVPP 245245

Query: 425    LCLVIGSRLDADI-HTNTCRLAFHGILLHGLED 456
                 VI SRLD  +  +N CR+AF+G ++  L++
Sbjct: 245244 FSHVICSRLDTTVSQSNVCRIAFYGPVVATLDN 245146



 Score = 58.9 bits (141), Expect(3) = 3e-74
 Identities = 30/82 (36%), Positives = 48/82 (58%), Gaps = 1/82 (1%)
 Frame = -1

Query: 464    LPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGE-LGIIDSAF 522
              L +L + K+K +HGLVE+  +    I + LF+KETN+ +F GL V       LG ID AF
Sbjct: 245092 LSKLHIGKIKERHGLVEKCGEHGVAIVKDLFRKETNLDIFKGLTVQNERSRALGAIDGAF 244913

Query: 523    GQSGKFKIHIPGGLSPESKKIL 544
              G++GK ++    G +    +++
Sbjct: 244912 GKTGKCRVQFTDGSTQPGDRLV 244847



 Score = 47.4 bits (111), Expect(2) = 4e-07
 Identities = 25/49 (51%), Positives = 32/49 (65%)
 Frame = -2

Query: 15     HIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
              ++DSGKT+L R+LST  STAA DK PQS+ RG          S P+P+R
Sbjct: 246411 YVDSGKTSLVRSLSTHLSTAALDKNPQSKARGTL------AASPPMPSR 246283



 Score = 26.2 bits (56), Expect(2) = 4e-07
 Identities = 10/18 (55%), Positives = 14/18 (77%)
 Frame = -3

Query: 6      VNVNVGVLGHIDSGKTAL 23
              VNVNVG+LGH+ +   A+
Sbjct: 246488 VNVNVGILGHVGTSLFAM 246435


>gb|GG743902.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.20, whole
              genome shotgun sequence
          Length = 492046

 Score = 89.7 bits (221), Expect = 9e-18
 Identities = 84/331 (25%), Positives = 149/331 (45%), Gaps = 37/331 (11%)
 Frame = +3

Query: 4      RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSV----P 59
              R+  +N+G +GH+  GK+ + +A+S    T  F  +    ER IT+ LG++   +    P
Sbjct: 262986 RQATINIGTIGHVAHGKSTVVKAISGV-QTVRFKNE---LERNITIKLGYANAKIYRSEP 263153

Query: 60     LPARLRSSL--PEFQAAPEAEP-----EPGEPLL---QVTLVDCPGHASLIRTIIGGAQI 109
               PA+   +   P + A   ++P     E G        V+ VDCPGH  L+ T++ GA +
Sbjct: 263154 APAKADEAPTGPMYSAFGSSKPDRFTDEKGVTWALQRHVSFVDCPGHDILMATMLNGAAV 263333

Query: 110    IDLMMLVIDVTKGM-QTQSAECLVIGQI-ACQKLVVVLNKIDLLPEGKRQAAIDKMTKKM 167
              +D  +L++   +   Q Q++E L   +I   Q ++++ NK+DL+     +A  +    ++
Sbjct: 263334 MDAALLLVAGNEPCPQPQTSEHLAAVEIMRLQNIIILQNKVDLIKPDAAKAQYE----QV 263501

Query: 168    QKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMS 227
              ++ +  T    APIIP++A               I  + E +   I IP RD      + 
Sbjct: 263502 KQFVAGTVAHDAPIIPISA----------VLKYNIDVVCEYIVRNIPIPRRDFVSKPKLI 263651

Query: 228    VDHCFSIKG--------QGTVMTGTILSGSISLGDSVE-------------IPALKVVKK 266
              V   F +          QG V  G+IL G + +GD +E             +    +  +
Sbjct: 263652 VIRSFDVNKPGQDVENLQGGVAGGSILQGVLRIGDEIEVRPGIYSKDEDGKVQCTPIFSR 263831

Query: 267    VKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297
              + S++  H  +  A+ G  +G+  T  DP L
Sbjct: 263832 IVSLKAEHNELQYAVPGGLIGV-GTLIDPTL 263921


>gb|GG743933.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.51, whole
              genome shotgun sequence
          Length = 226637

 Score = 61.6 bits (148), Expect = 3e-09
 Identities = 68/291 (23%), Positives = 123/291 (42%), Gaps = 28/291 (9%)
 Frame = +2

Query: 12     VLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEF 71
              ++GH+D+GKT L   +  T       K   +   GIT  +G + F V        ++ + 
Sbjct: 143015 IMGHVDTGKTKLLDNIRKT-------KVQDAEAGGITQQIGATFFPV-------EAIKQK 143152

Query: 72     QAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL 131
               AA +        L  + ++D PGH S       G+ + D+ +LV+D+  G++ Q+ E L
Sbjct: 143153 TAALQKVTNLQYKLPGLLVIDTPGHESFTNLRSRGSSLCDIAILVVDIMHGLEPQTLESL 143332

Query: 132    VIGQIACQKLVVVLNKIDLLPEGKR---QAAIDKMTKKMQKTLENTKFRGAPIIPVAAKP 188
               + +      VV LNKID     K        D +  + +  +   + R   I+   A+ 
Sbjct: 143333 RLLRAKRAPFVVALNKIDRCYSWKTCPDMPIRDALALQNEHVVREFEDRAKKIMVEFAEQ 143512

Query: 189    G--------GPEAPETE--------APQGIPELIELLTSQISIPTRDPSGPFLMSVD--- 229
              G          +   T         + +G+P+L+ +LT      T++     L  VD   
Sbjct: 143513 GLNAEVYWRNKDLGRTVSLIPTSAISGEGVPDLLMMLTKL----TQERMAKSLAFVDILQ 143680

Query: 230    ----HCFSIKGQGTVMTGTILSGSISLGDSVEIPALK--VVKKVKSMQMFH 274
                      I+G GT +   +++GS+S G ++ +  L+  VV  ++S+   H
Sbjct: 143681 CTVLEVKVIEGLGTTIDTILVNGSLSEGQTIVVCTLEGPVVSTIRSLLTPH 143833


>gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.50, whole
             genome shotgun sequence
          Length = 253698

 Score = 61.2 bits (147), Expect = 4e-09
 Identities = 41/124 (33%), Positives = 65/124 (52%), Gaps = 6/124 (4%)
 Frame = +1

Query: 204   ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
             EL+  +   I  PTRD   PFL+ V+  FSI G+GTV++G + +G I+ GD VE+  ++ 
Sbjct: 33673 ELMNQVDEYIPDPTRDFEKPFLLPVEDVFSISGRGTVVSGRVETGVINTGDEVELVGIRD 33852

Query: 264   V---KKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERG-LVCAPESL--HTVHAALI 317
             V        ++MF   +     GD +G+ +       + RG ++C P S+  HT   A +
Sbjct: 33853 VPTKTTCTGVEMFRKSLDRGQAGDNVGVLLRGLKRDDVLRGQVLCKPGSISPHTKVEAGV 34032

Query: 318   SVEK 321
              V K
Sbjct: 34033 YVLK 34044


>gb|GG743889.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.7, whole
              genome shotgun sequence
          Length = 943373

 Score = 51.2 bits (121), Expect = 4e-06
 Identities = 39/151 (25%), Positives = 63/151 (41%), Gaps = 10/151 (6%)
 Frame = -2

Query: 9      NVGVLGHIDSGKTALARALSTTASTAAF---------DKQPQSRERGITL-DLGFSCFSV 58
              N+ V+ H+D GK+ L  AL   A   +          D +   ++RGIT+   G S +  
Sbjct: 368110 NISVIAHVDHGKSTLTDALVAKAGIISHGAAGGARYTDTRDDEKDRGITIKSTGLSMYF- 367934

Query: 59     PLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVID 118
                                 E   G+PLL V L+D PGH      +    ++ D  ++V+D
Sbjct: 367933 -------------------ETAKGDPLL-VNLIDSPGHVDFSSEVTAALRVTDGALVVVD 367814

Query: 119    VTKGMQTQSAECLVIGQIACQKLVVVLNKID 149
                 G+  Q+   L        K V+++NK+D
Sbjct: 367813 AIDGVCVQTETVLRQAMAERVKPVLMVNKVD 367721


  Database: S.parasitica/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 53,132,636
  Number of sequences in database:  1445
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,604,091
Number of Sequences: 1445
Number of extensions: 633576
Number of successful extensions: 3839
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 93
Number of HSP's that attempted gapping in prelim test: 2602
Number of HSP's gapped (non-prelim): 2215
length of query: 596
length of database: 17,710,878
effective HSP length: 109
effective length of query: 487
effective length of database: 17,553,373
effective search space: 8548492651
effective search space used: 8548492651
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 117 (49.7 bits)