TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: P.tricornutum/genome.fa 
           31 sequences; 23,733,684 total letters

Searching...............................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...   404   e-113
gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    95   1e-19
gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    77   3e-14
gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    75   1e-13
gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    58   1e-08
gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    54   2e-07
gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    54   3e-07
gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    50   3e-06
gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ...    50   4e-06

>gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun
              sequence
          Length = 2535400

 Score =  404 bits (1039), Expect = e-113
 Identities = 236/599 (39%), Positives = 338/599 (56%), Gaps = 52/599 (8%)
 Frame = +2

Query: 6      VNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLR 65
              +NVN+GVLGH+DSGKT+L +ALST  STAA DK  QSR+RG+TLDLGFSCF + +P+ L+
Sbjct: 950720 LNVNLGVLGHVDSGKTSLVKALSTLLSTAALDKSKQSRQRGMTLDLGFSCFFLDMPSHLQ 950899

Query: 66     SSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQT 125
              +S P+            +  LQ+TLVDCPGHASLIRTIIGGAQIID+++LV+D  KG Q 
Sbjct: 950900 ASYPD------------KKNLQITLVDCPGHASLIRTIIGGAQIIDMVLLVVDAVKGWQA 951043

Query: 126    QSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVA 185
              Q+ ECLV+ ++    LVV LNK D  P  +R   +++   K++  L+ T+F  API+ VA
Sbjct: 951044 QTTECLVLAELTSPYLVVALNKADQFPPPERDQLLEQAAAKIRDRLKATRFANAPIVGVA 951223

Query: 186    AKPGGPEAPETEAPQGIPE----------LIELLTSQISIPTRDPSGPFLMSVDHCFSIK 235
              A  GG +           E          L++ L  ++  P R P G F  SVDHCFSI+
Sbjct: 951224 ACVGGEKVAAASEDMSKLEVRNETYNMETLVQTLQDKLPPPKRTPEGSFYFSVDHCFSIR 951403

Query: 236    GQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDP 295
              G+GTV+TGT+L+G+ S+ D +E P L + +K+KS+QMF   +    QGDR G+CV+  D 
Sbjct: 951404 GRGTVLTGTVLNGAASVNDVLEFPTLGLDRKIKSIQMFKRQVQRIQQGDRAGVCVSNLDA 951583

Query: 296    KLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFF---- 351
              KLLERG+   P ++  +  A+  V K+PY+ G L + +KFHI+VGH T+M  + F+    
Sbjct: 951584 KLLERGIAATPGAVQLLKGAIALVRKVPYYVGTLHSGSKFHISVGHTTIMATVTFWGAVE 951763

Query: 352    ------SPAP----DNFDQEPILDSFNFS-------------QEYLFQEQYLSKDLTPAV 388
                    +PA     +  D +P       S               + F+E+++ +D     
Sbjct: 951764 LLGKEQNPADLSVVNPVDTKPSKRYVGSSSLGGDADMAGLPRMTFSFEEEFIHQD----- 951928

Query: 389    TDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHT----NTCRL 444
                 D  +   GQ +  H     WAL++F+ PV CP   LVIGSRLD   ++    + CRL
Sbjct: 951929 ---DLLESLDGQESLLH-----WALLDFQIPVHCPMDSLVIGSRLDTVDNSTGSASACRL 952084

Query: 445    AFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDD-----------YSVIGRSL 493
              AF G L+  ++        + +L++Y  K + G + R  D            Y V G  L
Sbjct: 952085 AFSGRLMQSVD----PGKDISKLRLYTPKERRGTISRLGDPHKRTDDDKLVRYEVFGSDL 952252

Query: 494    FKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRA 552
              FKKETN++ F G+KV     E+G I S+FG SGK+++  P G   +  + L    K+ A
Sbjct: 952253 FKKETNMKAFFGMKVQTERNEIGEIKSSFGTSGKYRVFFPAGTEAKEGEPLILRFKRFA 952429


>gb|CM000623.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 21, whole genome shotgun
              sequence
          Length = 662217

 Score = 95.1 bits (235), Expect = 1e-19
 Identities = 66/224 (29%), Positives = 115/224 (51%), Gaps = 6/224 (2%)
 Frame = +3

Query: 94     PGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIG-QIACQKLVVVLNKIDLLP 152
              PGHA  ++ +I GA  +D  +LV+  T G   Q+ E +++  Q+    LVV LNK+DL+ 
Sbjct: 404019 PGHADYVKNMITGAAQMDGGILVVAATDGPMPQTREHILLAKQVGIPNLVVFLNKVDLVD 404198

Query: 153    EGKRQAAIDKMTKKMQKTLENTKFRG--APIIPVAAKPGGPEAPETEAPQGIPELIELLT 210
              +   +  ++ +  ++++ L+   F G   PII  +A             + I EL+  + 
Sbjct: 404199 D---EELLELVEMEIRELLDFYDFPGDDIPIIRGSALAAAEGRNPEIGSEKILELMAAVD 404369

Query: 211    SQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKV--K 268
               +I  P RD    FLM ++  FSI G+GTV+TG +  G +++GD +++  L    K    
Sbjct: 404370 EKIPEPMRDLDKDFLMPIEDVFSIAGRGTVVTGRVQQGKVNVGDELDVIGLDQNHKTICT 404549

Query: 269    SMQMFHMPITSAMQGDRLGICVTQFDPKLLERG-LVCAPESLHT 311
               ++MF   +   M GD +G  +     + + RG ++C P S+ T
Sbjct: 404550 GVEMFKKLLDFGMAGDNIGALLRGLKREDVRRGQILCKPGSMKT 404681


>gb|CM000625.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 23, whole genome
             shotgun sequence
          Length = 512847

 Score = 77.0 bits (188), Expect = 3e-14
 Identities = 87/354 (24%), Positives = 151/354 (42%), Gaps = 60/354 (16%)
 Frame = +2

Query: 4     RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSV--PLP 61
             R+  +NVG +GH+  GK+ + +A+S    T  F  +    ER IT+ LG++   +    P
Sbjct: 45551 RQATINVGTIGHVAHGKSTVVKAISGV-QTVRFKNE---LERNITIKLGYANAKIYQGQP 45718

Query: 62    ARLRSSLPEFQAAPEAEPEPG--------------EPLL--------------------- 86
                  +L + + A     E                 PL                      
Sbjct: 45719 VVSEENLHDNEDASTTTAETPLDADGTVHNTTIATGPLYTSRGSSHADIFTEGGRTYHLR 45898

Query: 87    -QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQI-ACQKLVV 143
               V+ VDCPGH  L+ T++ GA ++D  +L+I   +   Q Q++E L   +I   + +++
Sbjct: 45899 RHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNETCPQPQTSEHLAAVEIMRLEHILI 46078

Query: 144   VLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIP 203
             + NK+DL+   K  AA+ +  ++++K +  T    API+P++A               + 
Sbjct: 46079 LQNKVDLV---KPDAAVAQQ-EQIRKFVAGTVADAAPILPISA----------VLRYNMD 46216

Query: 204   ELIELLTSQISIPTRDPSGPFLMSVDHCFSI--------KGQGTVMTGTILSGSISLGDS 255
              L E L  +I +P RD +    + V   F +        K QG V  G+IL G + +GD 
Sbjct: 46217 VLCEYLIRRIPLPVRDFTSQPRLIVIRSFDVNRPGQDVSKLQGGVAGGSILQGVLRVGDE 46396

Query: 256   VE------------IPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297
             +E            I  + +  K+ S+      +  A+ G  +G+  T+ DP L
Sbjct: 46397 IEVRPGIVHKQDDKIVCVPIFSKISSLYAEQNDLQFAVPGGLIGV-GTKIDPTL 46555


>gb|CM000615.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 13, whole genome shotgun
              sequence
          Length = 887524

 Score = 74.7 bits (182), Expect = 1e-13
 Identities = 67/282 (23%), Positives = 115/282 (40%), Gaps = 13/282 (4%)
 Frame = +3

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              D     RERGIT+D+    F                           P    T++D PGH
Sbjct: 601719 DNLKAERERGITIDIALWKFE-------------------------SPKYSFTVIDAPGH 601823

Query: 97     ASLIRTIIGGAQIIDLMMLVID---------VTKGMQTQSAECLVIGQIACQKLVVVLNK 147
                 I+ +I G    D+ +LVID         ++K  QT+    L+   +  ++++V +NK
Sbjct: 601824 RDFIKNMITGTSQADVAVLVIDSSQGGFEAGISKDGQTRE-HALLAYTLGVKQMIVAMNK 602000

Query: 148    IDLLPEGKRQAAIDKMTKKMQKTLENTKFR--GAPIIPVAAKPGG--PEAPETEAPQGIP 203
              +D       +    ++  ++   L+   ++    P +P++   G    E     A    P
Sbjct: 602001 MDDKTVKYAEDRYTEIKNEVSAYLKKVGYKPMKIPFVPISGWEGDNMVEKSTNMAWYKGP 602180

Query: 204    ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
               L+E L S ++ P R       + +   + I G GTV  G + +G I  G         +
Sbjct: 602181 YLLEALDS-VTPPKRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVIKPGIHAMFAPSGI 602357

Query: 264    VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
              + ++KS++M H  +  A+ GD +G  V     K L RG V +
Sbjct: 602358 IAEIKSVEMHHESLPEAVPGDNVGFNVKNVAVKDLRRGFVAS 602483


>gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun
              sequence
          Length = 1098047

 Score = 58.2 bits (139), Expect = 1e-08
 Identities = 72/315 (22%), Positives = 129/315 (40%), Gaps = 33/315 (10%)
 Frame = -1

Query: 5      RVNVNVGVLGHIDSGKTALARALSTTASTA------AFDKQPQSRERGITLDLGFSCFSV 58
              R ++N+  +GH+D+GK+ L+  +              ++K+ + R R    +  F  F +
Sbjct: 710933 REHLNLVFIGHVDAGKSTLSGNILYLTDNVDKRTIERYEKEAKERNR----ESWFLAFIM 710766

Query: 59     PLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVID 118
                    R+     +   +A+ E    L + T++D PGH + +  +I GA   D+ +LVI 
Sbjct: 710765 DTNEEERAKGKTVEVG-KAQFETD--LKRYTILDAPGHKNYVPNMIQGASQADVGVLVIS 710595

Query: 119    VTKGM---------QTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQK 169
                KG          QT+    ++   +    LVV +NK+D      ++   D+   K++ 
Sbjct: 710594 ARKGEFETGFDRGGQTRE-HAMLAKTLGVSYLVVAVNKMDEPTVQWKKERFDECVTKIRP 710418

Query: 170    TLENTKF---RGAPIIPVAAKPGGPEAPE---------------TEAPQGIPELIELLTS 211
               L++  F   R    IP++   G     E                E     P L+ LL +
Sbjct: 710417 FLKSCGFVIKREVKFIPISGLSGANVKDEVALSECSWWKEYYTSNENNTSAPTLLSLLDT 710238

Query: 212    QISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQ 271
               + I  RDP  P  + V   ++   +G +  G + SG I  G  V I   +   KV  + 
Sbjct: 710237 -LEIAGRDPEAPLRIPVLDRYT--DRGIISMGKVESGIIRPGMKVTIMPTRNKYKVDEVW 710067

Query: 272    MFHMPITSAMQGDRL 286
                  P++ A  G+ +
Sbjct: 710066 ANEDPVSGARPGENV 710022


>gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 4, whole genome shotgun
              sequence
          Length = 1360148

 Score = 54.3 bits (129), Expect = 2e-07
 Identities = 60/248 (24%), Positives = 100/248 (40%), Gaps = 3/248 (1%)
 Frame = -1

Query: 10     VGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLP 69
              V ++GH+D GKT+L   +  T  TA           GIT                   + 
Sbjct: 669560 VTIMGHVDHGKTSLLDTIRKTQVTAG-------EAGGIT-----------------QHIA 669453

Query: 70     EFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE 129
               +Q     +         +T VD PGHA+       GA I D+++LV+    G++ Q+A+
Sbjct: 669452 AYQVTRNDQT--------ITFVDTPGHAAFTDMRERGANITDIIVLVVAADDGVKQQTAD 669297

Query: 130    CLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIP-VAAKP 188
               +   + A   L+V +NK DL      +     MT+     +   +F G  +   ++AK 
Sbjct: 669296 SIACARQAGVPLIVAINKCDLETADPTKV----MTELTSYDVLTEEFGGDVLSSQISAKK 669129

Query: 189    GGPEAPETEAPQGIPELIE--LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTIL 246
              G            I +L++  +L ++I     +P       V      KG GTV T  + 
Sbjct: 669128 G----------TNIDDLLDKIMLQAEIQDIKANPDRDAECIVVEANVEKGLGTVATTLVQ 668979

Query: 247    SGSISLGD 254
               G++ +GD
Sbjct: 668978 KGTLRVGD 668955


>gb|CM000629.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 27, whole genome shotgun
              sequence
          Length = 404298

 Score = 53.9 bits (128), Expect = 3e-07
 Identities = 39/141 (27%), Positives = 65/141 (46%), Gaps = 1/141 (0%)
 Frame = -2

Query: 10     VGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLP 69
              V ++GH+D+GKT L   +  T          +    GIT  +G + F       L   + 
Sbjct: 276773 VVIMGHVDTGKTKLLDKIRKT-------NVQEGEAGGITQQIGATYFE---KKTLEQQVS 276624

Query: 70     EFQAAPEAEPE-PGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSA 128
              +  A    E + PG     + ++D PGH S       G+ + D+ +LV+D+  G++ Q+ 
Sbjct: 276623 KLNATENVELKVPG-----MLVIDTPGHESFTNLRSRGSSLCDVAILVVDLMHGLEQQTI 276459

Query: 129    ECLVIGQIACQKLVVVLNKID 149
              E L + +      VV LNK+D
Sbjct: 276458 ESLTMLRKRGVPFVVALNKVD 276396


>gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 8, whole genome shotgun
              sequence
          Length = 1007773

 Score = 50.4 bits (119), Expect = 3e-06
 Identities = 45/163 (27%), Positives = 71/163 (43%), Gaps = 22/163 (13%)
 Frame = +1

Query: 9      NVGVLGHIDSGKTALARAL--------STTASTAAF-DKQPQSRERGITL-DLGFSCF-- 56
              ++ V+ H+D GKT L  +L        S  A  A + D +    ERGIT+   G S F  
Sbjct: 702058 SMSVIAHVDHGKTTLTDSLVQKAGIISSKAAGGARYTDTRADEAERGITIKSTGISMFFE 702237

Query: 57     ---SVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVT-------LVDCPGHASLIRTIIGG 106
                      A L     E +AA  AE +  E  +Q++       L+D PGH      +   
Sbjct: 702238 YDMKAGEVAGLSKEDEEARAASIAEKQDSEANVQISEKSYLINLIDSPGHVDFSSEVTAA 702417

Query: 107    AQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149
               ++ D  ++V+D   G+  Q+   L        K V+++NK+D
Sbjct: 702418 LRVTDGALVVVDTIDGVCVQTETVLRQAIAERVKPVLMVNKVD 702546


>gb|CM000620.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 18, whole genome shotgun
              sequence
          Length = 702471

 Score = 50.1 bits (118), Expect = 4e-06
 Identities = 42/200 (21%), Positives = 87/200 (43%), Gaps = 3/200 (1%)
 Frame = -3

Query: 88     VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147
              + L+D PGH      +       +  +LV+D ++G++ Q+   + +      +++ +LNK
Sbjct: 516373 LNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGIEAQTLANVYLALENDLEIIPILNK 516194

Query: 148    IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIE 207
              IDL       A  +++ ++++ T+       + I+  +AK G           GI +++E
Sbjct: 516193 IDL-----PAADPERVAEEIEATI---GLDCSGIVHASAKTG----------IGIDDILE 516068

Query: 208    LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV---V 264
               +   +  P     GPF   +   +    +G ++   ++ G +S GD V   A      V
Sbjct: 516067 RIVQMVPPPPAATGGPFRALIFDSYYDAYRGVIVFFRVMDGEVSQGDKVRFLASDAEHDV 515888

Query: 265    KKVKSMQMFHMPITSAMQGD 284
               +V  MQ   +P+     G+
Sbjct: 515887 TEVGIMQPNQVPVDCLHAGE 515828


  Database: P.tricornutum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 23,733,684
  Number of sequences in database:  31
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,965,097
Number of Sequences: 31
Number of extensions: 252396
Number of successful extensions: 1350
Number of sequences better than 1.0e-05: 9
Number of HSP's better than  0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1102
Number of HSP's gapped (non-prelim): 408
length of query: 596
length of database: 7,911,228
effective HSP length: 104
effective length of query: 492
effective length of database: 7,908,004
effective search space: 3890737968
effective search space used: 3890737968
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 114 (48.5 bits)