TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: P.pallidum/genome.fa 
           42 sequences; 32,942,533 total letters

Searching..........................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GL290995.1| Polysphondylium pallidum PN500 unplaced genomic s...   183   e-117
gb|GL291006.1| Polysphondylium pallidum PN500 unplaced genomic s...   124   3e-28
gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic s...    72   2e-12
gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic s...    71   2e-12
gb|GL290988.1| Polysphondylium pallidum PN500 unplaced genomic s...    64   3e-10
gb|GL290986.1| Polysphondylium pallidum PN500 unplaced genomic s...    57   4e-08

>gb|GL290995.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold13,
              whole genome shotgun sequence
          Length = 1052162

 Score =  183 bits (464), Expect(4) = e-117
 Identities = 84/181 (46%), Positives = 124/181 (68%), Gaps = 7/181 (3%)
 Frame = -1

Query: 163    MTKKMQKTLENTKFRGAPIIPVAAKPGGPE--APETEAPQG-----IPELIELLTSQISI 215
              MT K++K LE T F+ +P + ++A P      AP + A  G     +  LI+ LT+ + +
Sbjct: 301682 MTNKLKKVLEKTCFKDSPYVAISANPSSSSDAAPASAAATGDNNIGVDRLIKELTNYVEL 301503

Query: 216    PTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHM 275
              P RD  GPFL   DHCF IKGQG+VMTGT+L GS+ +  ++ IP L + KKVKSMQMFH 
Sbjct: 301502 PRRDDQGPFLFEFDHCFQIKGQGSVMTGTVLRGSVEINQNIHIPQLNIEKKVKSMQMFHK 301323

Query: 276    PITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKF 335
              P+  A QGDR+GICVTQ D  LLERGL+C P+++  ++ A++++EK+ +++  ++TK++F
Sbjct: 301322 PVKKASQGDRVGICVTQLDANLLERGLLCTPKTVPLLNGAIVAIEKVRFYKNEVKTKSQF 301143

Query: 336    H 336
              H
Sbjct: 301142 H 301140



 Score =  116 bits (291), Expect(4) = e-117
 Identities = 61/101 (60%), Positives = 76/101 (75%)
 Frame = -1

Query: 6      VNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLR 65
              +N+N+G++GH+DSGKT+LA+ALST+ STAA DK P S+ERGITLDLGFS F   LP RL+
Sbjct: 302285 LNINIGIMGHVDSGKTSLAKALSTSLSTAALDKSPASQERGITLDLGFSAFHSELPDRLK 302106

Query: 66     SSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGG 106
              S  P+  A    +       +Q TLVDCPGHASLI+TIIGG
Sbjct: 302105 SH-PDVIANNYNQ-------IQYTLVDCPGHASLIKTIIGG 302007



 Score =  103 bits (258), Expect(4) = e-117
 Identities = 68/253 (26%), Positives = 115/253 (45%), Gaps = 46/253 (18%)
 Frame = -2

Query: 337    ITVGHETVMGRLMFFS----------PAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTP 386
              +T+GH TV+   +FF             P+   +   L+ FN   EY + +         
Sbjct: 301063 VTIGHSTVIATAIFFGNKKQQTEQQQQQPETMIENMTLNQFNSKLEYSYLDSL------- 300905

Query: 387    AVTDNDEADKKAGQATEGHCP-RQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLA 445
                            AT    P   Q+AL+ FE PV CP   ++IGS+LD ++ T++CR+A
Sbjct: 300904 -------------NATGQEYPVGSQYALLTFEHPVLCPLNSVIIGSKLDVNLDTSSCRIA 300764

Query: 446    FHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVG 505
              FHG L+ G++  N        L +YK+K K G +ER   + ++IG+++F K+T++  F+G
Sbjct: 300763 FHGELVEGIDVTN-KQLLNNHLHIYKIKAKEGQIERIHSESTLIGKNIFGKDTDLSKFIG 300587

Query: 506    LKV-----------------------------------HLSTGELGIIDSAFGQSGKFKI 530
              +KV                                   +   G +G+++S FG++GK KI
Sbjct: 300586 MKVIFDNGMFDVLLLL*NNKRKNKSS*FNLKN*LII*CYFKIGVVGLLESGFGKTGKVKI 300407

Query: 531    HIPGGLSPESKKI 543
               +P  +   + K+
Sbjct: 300406 TLPASVDINTIKV 300368



 Score = 80.9 bits (198), Expect(4) = e-117
 Identities = 38/56 (67%), Positives = 47/56 (83%)
 Frame = -3

Query: 106    GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAID 161
              G+QIID+M LVID+TKG+QTQ+AEC+VIG+I C+K +VVLNKID LP   RQ  ID
Sbjct: 301935 GSQIIDMMFLVIDITKGIQTQTAECIVIGEITCKKAIVVLNKIDQLPIESRQNKID 301768


>gb|GL291006.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig9,
              whole genome shotgun sequence
          Length = 1029409

 Score =  124 bits (310), Expect = 3e-28
 Identities = 97/324 (29%), Positives = 153/324 (47%), Gaps = 17/324 (5%)
 Frame = +3

Query: 7      NVNVGVLGHIDSGKTALA---------RALSTTASTAAFDKQPQSRERGITLDLGFSCFS 57
              +VNVG +GH+D GKT L          R L+   S +  DK P+ R+RGIT++       
Sbjct: 826653 HVNVGTIGHVDHGKTTLTAAITKCLSDRGLANFKSYSQIDKSPEERQRGITIN------- 826811

Query: 58     VPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVI 117
                      +S  E+++                 +DCPGH   I+ +I GA  +D  +LV+
Sbjct: 826812 --------ASHIEYESDNR----------HYAHIDCPGHQHYIKNMITGAAQMDGAILVV 826937

Query: 118    DVTKGMQTQSAECLVIG-QIACQKLVVVLNKIDLLPEGKRQAA---IDKMTKKMQKTLEN 173
                  G Q Q+ E +++  ++   K+VV LNK+D       +     + ++  K     E 
Sbjct: 826938 SAPDGPQEQTREHVILSREVGIPKIVVFLNKMDNADPDLVEIVEMEVRELLAKYGFDGEA 827117

Query: 174    TKF-RGAPIIPVAAKPGGPEAPETEAPQ-GIPELIELLTSQISIPTRDPSGPFLMSVDHC 231
              T F +GA  + +A  P  P    TE  +  I +L+ +L ++I +P R    PFLM V+  
Sbjct: 827118 TPFVKGAAAVALAEDPASP----TEFGRLAIDKLVSVLDNEIPLPKRAIDKPFLMPVEEV 827285

Query: 232    FSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVK--SMQMFHMPITSAMQGDRLGIC 289
              FSI G+GTV TG I  G + +GD V I  +K V KV    ++MF   +  A  G+ +G+ 
Sbjct: 827286 FSISGRGTVATGRIDQGVVKVGDEVAIVGIKPVPKVSVTGLEMFGKLLDFAQAGENVGVL 827465

Query: 290    VTQFDPKLLERGLVCAPESLHTVH 313
              +     + + RG V +       H
Sbjct: 827466 LRGLKREDVVRGEVISKPGTIKAH 827537


>gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold2,
              whole genome shotgun sequence
          Length = 3242543

 Score = 71.6 bits (174), Expect = 2e-12
 Identities = 67/281 (23%), Positives = 114/281 (40%), Gaps = 14/281 (4%)
 Frame = -3

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              DK    RERGIT+D+    F                                T++D PGH
Sbjct: 924645 DKLKAERERGITIDIALWKFETNK-------------------------YYFTIIDAPGH 924541

Query: 97     ASLIRTIIGGAQIIDLMMLVI---------DVTKGMQTQSAECLVIGQIACQKLVVVLNK 147
                 I+ +I G    D  +LVI          + K  QT+    L+   +  ++++V +NK
Sbjct: 924540 RDFIKNMITGTSQADCAVLVIASPTGEFEAGIAKNGQTRE-HALLAYTLGVKQMIVAINK 924364

Query: 148    IDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPI--IPVAAKPGGPEAPE-TEAP--QGI 202
              +D       +    ++ K+    ++   +    +  +P++   G       T  P  +G 
Sbjct: 924363 MDEKSTNYSEGRYSEIVKETSSFIKKIGYNPEKVAFVPISGWNGDNMLERSTNMPWYKG- 924187

Query: 203    PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALK 262
              P L+E L + +  P R    P  + +   + I G GTV  G + +G +  G  V      
Sbjct: 924186 PTLLEALDAIVE-PKRPVDKPLRIPLQDVYKIGGIGTVPVGRVETGILKPGMVVTFAPAG 924010

Query: 263    VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303
              +  +VKS++M H  +T A  GD +G  V     K ++RG+V
Sbjct: 924009 LSTEVKSVEMHHEQLTQAAPGDNVGFNVKNLSVKDIKRGMV 923887



 Score = 64.7 bits (156), Expect = 2e-10
 Identities = 67/252 (26%), Positives = 114/252 (45%), Gaps = 23/252 (9%)
 Frame = -3

Query: 95      GHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLP 152
               GH  L+ T++ GA ++D  ML+I   +   Q Q+AE +   +I   + +++L NKIDL+ 
Sbjct: 2990652 GHDVLMATMLNGAAVMDAAMLLIAGNESCPQPQTAEHIAAIEIMNLRNIIILQNKIDLVK 2990473

Query: 153     EGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQ 212
               E    AA  +   ++ K ++ T   GAPIIP++A          +    I  + E +  +
Sbjct: 2990472 E----AAAKEQYGQILKFIQGTVAEGAPIIPISA----------QMKYNIDVICEYIVKK 2990335

Query: 213     ISIPTRDPSGPFLMSVDHCFSIKG--------QGTVMTGTILSGSISLGDSVEIPALKVV 264
               I IP RD +    + V   F +          +G V  G+I+ G + +GD +E+    + 
Sbjct: 2990334 IPIPVRDFTSEPRLIVIRSFDVNKPGAKVDELKGGVAGGSIIKGVLRVGDEIEVRPGVIT 2990155

Query: 265     K-------------KVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHT 311
               K             ++ S+      +  A+ G  +G+  T+ DP L      C  + L  
Sbjct: 2990154 KDLDGRMRCSPLFSRIISLYAEENDLQYAVPGGLIGV-GTKIDPSL------CRADRL-- 2990002

Query: 312     VHAALISVEKIP 323
               V   L SV K+P
Sbjct: 2990001 VGQVLGSVGKLP 2989966


>gb|GL290993.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold11,
               whole genome shotgun sequence
          Length = 1505626

 Score = 71.2 bits (173), Expect = 2e-12
 Identities = 64/264 (24%), Positives = 108/264 (40%), Gaps = 13/264 (4%)
 Frame = +3

Query: 37      DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
               D     RERGIT+D     F +                             VT++D PGH
Sbjct: 1128639 DNLKSERERGITIDCSLMRFDIDK-------------------------YDVTIIDAPGH 1128743

Query: 97      ASLIRTIIGGAQIIDLMMLVIDVTKG--------MQTQSAECLVIGQIACQKLVVVLNKI 148
                  I+ +I G+   D  +LV+   KG          +     L+   +  ++L+V +NK+
Sbjct: 1128744 KDFIKNMITGSSQADCAVLVVSSVKGEFEAGIDKKGSTREHALLAYTLGVRQLIVAVNKM 1128923

Query: 149     D-LLPEGKRQAAIDKMTKKMQKTLENTKFRG--APIIPVAAKPGGPEAPE-TEAP-QGIP 203
               D        +A  +++ K+    ++   +     P IP++A  G     + T+ P    P
Sbjct: 1128924 DDEKTTNYSEARFNEIVKETSSFIKKIGYNPEKVPFIPLSAWNGDNMLEKSTKMPWYNGP 1129103

Query: 204     ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
                L E L S +  P R    P  + +   + I G GTV  G + +G +  G  V I  +  
Sbjct: 1129104 TLYEALNSIVE-PKRPVDKPLRLPIQDVYKIGGIGTVAVGRVETGVMKAGQPVVIAPVNK 1129280

Query: 264     VKKVKSMQMFHMPITSAMQGDRLG 287
               V +VKS++  H+ +  A+ G  +G
Sbjct: 1129281 VLEVKSIERHHVQVDQAIPGFNVG 1129352


>gb|GL290988.1| Polysphondylium pallidum PN500 unplaced genomic scaffold
            PPL_scaffold6, whole genome shotgun sequence
          Length = 2018088

 Score = 63.9 bits (154), Expect = 3e-10
 Identities = 72/333 (21%), Positives = 140/333 (42%), Gaps = 36/333 (10%)
 Frame = -2

Query: 7    NVNVGVLGHIDSGKTALA------------RALSTTASTA------------AFDKQPQS 42
            ++N+ V+GH+D+GK+               R +S     A              D+  + 
Sbjct: 7553 HLNMVVIGHVDAGKSTTMGHLLYKLGYVDQRTISKFEREANNIGKGSFHFAWVLDEHQEE 7374

Query: 43   RERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRT 102
            RERG+T+D+    F                   E E        ++TL+D PGH   +  
Sbjct: 7373 RERGVTMDVCVRYF-------------------ETEHR------KITLLDAPGHRDFVPN 7269

Query: 103  IIGGAQIIDLMMLVIDVTKGMQTQSAE------CLVIGQIACQKLVVVLNKIDLLPEGKR 156
            +I G    D+ +L+I+ ++     S+E       L+   +   +L+V +NK+DL+   + 
Sbjct: 7268 MISGTTQADVAILLINASEFEAGFSSEGQTKEHALLAKSLGIMQLIVAINKMDLVDWSEE 7089

Query: 157  QAAIDKMTKKMQKTLENTKF--RGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQI- 213
            +   + +T+ +++ L + KF  +    +P++   G     +   P+        L  QI 
Sbjct: 7088 R--YNYITETLKQFLVSAKFNEKNLYFMPISGFKGDNLIEKIADPRCSWYTGNTLVQQID 6915

Query: 214  --SIPTRDPSGPFLMSVDHCFSIKGQGTVMT-GTILSGSISLGDSVEIPALKVVKKVKSM 270
              S+  R  + PF M V+  +    +G+V+  G I +G + +GD + I     +  VK++
Sbjct: 6914 SFSVGERLINKPFRMGVNDIYKTNSKGSVLVGGKIEAGVLGVGDKLLISPGNELCTVKAI 6735

Query: 271  QMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303
            +  H     A+ GD + + +      +L  G +
Sbjct: 6734 RRAHNDSDWAVGGDNIDLSLAIDVTNILRLGSI 6636



 Score = 57.0 bits (136), Expect = 4e-08
 Identities = 41/145 (28%), Positives = 69/145 (47%)
 Frame = +3

Query: 12     VLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEF 71
              +LGH+D+GKT+L   + +T              RGIT  +G S   V        ++ E 
Sbjct: 513063 ILGHVDTGKTSLLDKIRSTNVQGG-------EARGITQQIGASFVPV-------ETIKEQ 513200

Query: 72     QAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL 131
                    + +    L  + L+D PGH S       G+ + DL +LVID+  G++ Q+ E +
Sbjct: 513201 TKGINEKLKVNFRLPGLLLIDTPGHESFNNLRSRGSGLCDLAILVIDIVHGIEKQTVESI 513380

Query: 132    VIGQIACQKLVVVLNKIDLLPEGKR 156
               + ++     VV LNK+D + E ++
Sbjct: 513381 NLLRMRKTPFVVALNKVDRVYEWQK 513455



 Score = 55.1 bits (131), Expect = 2e-07
 Identities = 63/292 (21%), Positives = 111/292 (38%), Gaps = 26/292 (8%)
 Frame = -3

Query: 9      NVGVLGHIDSGKTALARALSTTASTAA--------------------------FDKQPQS 42
              N  ++ HID GKT L+  L +   T                             DK    
Sbjct: 483673 NFSIIAHIDHGKTTLSTKLLSLTGTLPKNIYGSGEDSLDQSKQIKHDNRREQYLDKLQVE 483494

Query: 43     RERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRT 102
              +ERGIT+    SC  +                     E G+  L + L+D PGH      
Sbjct: 483493 KERGITVKAQ-SCSMI-----------------YRNREDGKRYL-LNLIDTPGHVDFNYE 483371

Query: 103    IIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDK 162
              +          +LV+D  +G+Q Q+     +   +  ++V V+NKIDL       A ++ 
Sbjct: 483370 VSRSLMACQGALLVVDACQGVQAQTMANYYLALDSGLEVVPVINKIDL-----PTANVEM 483206

Query: 163    MTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSG 222
              + ++++ T     F    +I V+AK G           GI +++  +  ++  P  D   
Sbjct: 483205 VKEQLKNTF---GFHPNDVICVSAKSG----------IGITDILPAVIDRVPPPQGDTKA 483065

Query: 223    PFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFH 274
              PF   +   +  + +G +    ++ GSI  GD ++  A     +V  + + H
Sbjct: 483064 PFKALLFDSWFDRFRGVICLINVVDGSIKKGDVIQSAASGQTYEVLDLGVMH 482909


>gb|GL290986.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold4,
               whole genome shotgun sequence
          Length = 1229170

 Score = 57.0 bits (136), Expect = 4e-08
 Identities = 62/238 (26%), Positives = 110/238 (46%), Gaps = 28/238 (11%)
 Frame = +3

Query: 88      VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQK-LVVVL 145
               V  +D P H  L+  +    + +D  MLVI   +   Q Q+A+ L I +I   K +++V 
Sbjct: 1003251 VCYIDYPTHNVLMGALFNCERFVDATMLVIASNEPCPQQQTADHLAIVEILKLKNIIIVQ 1003430

Query: 146     NKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAK-------------PGGPE 192
               NKIDL+   ++Q+  ++   ++Q+ ++ +   GAPIIP++A+                P 
Sbjct: 1003431 NKIDLV---RQQSRANEHHNQIQQFVKGSVAEGAPIIPISAQLKWNIDVLCEHVVTKIPT 1003601

Query: 193     APETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISL 252
               +P ++     P LI + +  ++ P R               +KG GTV  G +LSG + +
Sbjct: 1003602 SPISDNYNSAPHLIVVRSFNMNAPYRKAE-----------EMKG-GTV-RGLLLSGILRV 1003742

Query: 253     GDSVEIPALKVVK-------------KVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297
               GD +EI    + K             K+ S+      +  A+ G  +G+ +T+ DP L
Sbjct: 1003743 GDDIEIRPGIITKNHDGTFSCTPLFSKIVSLFAEDNELQYAVPGGLIGV-LTKLDPSL 1003913


  Database: P.pallidum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 32,942,533
  Number of sequences in database:  42
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,181,076
Number of Sequences: 42
Number of extensions: 242551
Number of successful extensions: 866
Number of sequences better than 1.0e-05: 6
Number of HSP's better than  0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 692
Number of HSP's gapped (non-prelim): 327
length of query: 596
length of database: 10,980,844
effective HSP length: 106
effective length of query: 490
effective length of database: 10,976,392
effective search space: 5378432080
effective search space used: 5378432080
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 116 (49.3 bits)