TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   294   4e-83
gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...   134   3e-31
gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    90   7e-18
gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    77   6e-14
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    77   6e-14
gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    77   6e-14
gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    76   1e-13
gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    64   5e-10
gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    59   1e-08
gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    59   1e-08
gb|GG738934.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    50   8e-06

>gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_11, whole genome
             shotgun sequence
          Length = 613270

 Score =  294 bits (753), Expect(2) = 4e-83
 Identities = 160/355 (45%), Positives = 220/355 (61%), Gaps = 30/355 (8%)
 Frame = -1

Query: 12    VLGHIDSG--------------KTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFS 57
             ++GH+DSG              KT+L +ALST ASTAAFDK PQS+ERGIT DLGFS F 
Sbjct: 66928 IIGHVDSGEFLMKFILMIYLKGKTSLCKALSTHASTAAFDKSPQSKERGITQDLGFSAFY 66749

Query: 58    VPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVI 117
                P      L E+              +Q TLVDCPGHASLIRTIIGGAQIID M+LVI
Sbjct: 66748 SDSP------LSEYDR------------IQYTLVDCPGHASLIRTIIGGAQIIDFMVLVI 66623

Query: 118   DVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTK-----------K 166
             D  KG+QTQ+AEC+VIG+I+C  L+V LNK+D++P  +R+  +DK++            +
Sbjct: 66622 DAVKGVQTQTAECIVIGEISCNNLIVALNKVDMIPADQREDRVDKVSNFKIYLGN*FSLQ 66443

Query: 167   MQKTL-----ENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPS 221
             ++K+L       TKF+  PI+ V A P   E+      QG+  LI      + I  +D +
Sbjct: 66442 IKKSLIKNVFSQTKFKNPPIVSVCADPR-EESESITKEQGLKNLIAEFQKHVKISEKDEN 66266

Query: 222   GPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAM 281
              PFL  VDHCF+IKG+GT++TGT+L GS+S+G +++IP++   KKVKS+QMF  P+  A+
Sbjct: 66265 EPFLYMVDHCFTIKGKGTIITGTVLQGSVSIGKTIQIPSIGEEKKVKSIQMFKTPVDKAI 66086

Query: 282   QGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFH 336
             +GDR+GI VT+ D K LERG  C    +  V   L +  +I +F+  ++  AK+H
Sbjct: 66085 KGDRIGIAVTKLDSKKLERGFACDKGYIEIVDTILANASRIRFFKKEIKNSAKYH 65921



 Score = 66.6 bits (161), Expect = 6e-11
 Identities = 38/100 (38%), Positives = 60/100 (60%), Gaps = 6/100 (6%)
 Frame = -3

Query: 437   IHTNTCRLAFHGILLHGLEDRNYA-DSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFK 495
             I  N CRLAF+G ++      N + +  +  L V+K K K  +V+R +D  ++IG++L  
Sbjct: 65615 IDANMCRLAFNGKVIKMWNKSNVSKEQLVNSLPVFKYKEKKAVVDRVVDANTIIGKNLLT 65436

Query: 496   KET-NIQLFVGLKVHLSTGEL----GIIDSAFGQSGKFKI 530
              +T ++Q F+G K+ +   +     GIIDS FGQSGKFK+
Sbjct: 65435 SKTQSLQFFIGKKIDILIDDQPRFSGIIDSPFGQSGKFKV 65316



 Score = 34.3 bits (77), Expect(2) = 4e-83
 Identities = 33/122 (27%), Positives = 48/122 (39%), Gaps = 9/122 (7%)
 Frame = -2

Query: 337   ITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADK 396
             +++GHETVMG +   + A        I D+   + EY   E+         +T+    +K
Sbjct: 65880 VSIGHETVMGNVSLIARAKKEGLSSNIFDT---TVEYSLLEE---------LTETTYQEK 65737

Query: 397   KAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADI---------HTNTCRLAFH 447
                           +  +EFEKPV C      I S+LD DI         +TN C     
Sbjct: 65736 DV------------FVKIEFEKPVCCYPNSNYIASKLDTDISKFFTIIFSNTNRCEYV*I 65593

Query: 448   GI 449
             GI
Sbjct: 65592 GI 65587


>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun
              sequence
          Length = 707703

 Score =  134 bits (337), Expect = 3e-31
 Identities = 98/320 (30%), Positives = 156/320 (48%), Gaps = 20/320 (6%)
 Frame = -2

Query: 7      NVNVGVLGHIDSGKTALARALSTTASTA-----------AFDKQPQSRERGITLDLGFSC 55
              ++N+G +GH+D GKT L  A++   +               DK P+ ++RGIT+      
Sbjct: 506453 HINIGTIGHVDHGKTTLTAAITKYLNELDPKANSFLAYDQIDKAPEEKQRGITIS----- 506289

Query: 56     FSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMML 115
                        +S  E+  A                +DCPGHA  I+ +I GA  +D  +L
Sbjct: 506288 ----------TSHIEYATATR----------HYAHIDCPGHADYIKNMITGAAQMDGSIL 506169

Query: 116    VIDVTKGMQTQSAECLVI-GQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENT 174
              VI    G   Q+ E L++  Q+  + +VV LNK+DL+P+ +    I+ + ++++ T++  
Sbjct: 506168 VISADDGPMLQTREHLLLCKQVGIKHIVVFLNKVDLVPDAEM---IEMVEEEIRDTVKQY 505998

Query: 175    KFRGAPIIPVAAKP-GGPEAPETE-APQGIPELIELLTSQISIPTRDPSGPFLMSVDHCF 232
               F G  I  +      G E   +E   Q I +L++ +   I  P RD + PFLM V+  F
Sbjct: 505997 GFDGDNISIIRGSALKGLEGDSSEIGTQAIQKLMDAVDKDIPEPERDINKPFLMPVEGVF 505818

Query: 233    SIKGQGTVMTGTILSGSISLGDSVEI-----PALKVVKKVKSMQMFHMPITSAMQGDRLG 287
              +I G+GTV TG +  G + LG  VE+      ALK    V  ++MFH  +     GD LG
Sbjct: 505817 TISGRGTVATGKVDQGQLKLGAEVEVVGFAPTALK--SSVTGIEMFHKLVDRGQAGDNLG 505644

Query: 288    ICVTQFDPKLLERG-LVCAP 306
              I +   D K ++RG ++C P
Sbjct: 505643 ILLRGIDKKQIKRGQVICKP 505584


>gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_28, whole genome shotgun
              sequence
          Length = 413107

 Score = 89.7 bits (221), Expect = 7e-18
 Identities = 83/298 (27%), Positives = 140/298 (46%), Gaps = 22/298 (7%)
 Frame = +3

Query: 4      RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
              R+  +N+G +GH+  GK+ + RA+S    T  F  +     R IT+ LG++   + L   
Sbjct: 112842 RQATINIGTIGHVAHGKSTVVRAISGV-KTIRFKNE---MVRNITIKLGYANAKIFLCRD 113009

Query: 64     LRSSLPEFQAAP----------EAEPEPGEPLL--QVTLVDCPGHASLIRTIIGGAQIID 111
               +   PE   +           E +   GE  L   V+ VDCPGH  L+ T++ GA ++D
Sbjct: 113010 PKCPRPECYTSQGSNHCDHFPCEVDDFKGEYELVRHVSFVDCPGHDILMATMLNGAAVMD 113189

Query: 112    LMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLPEGKRQAAIDKMTKKMQK 169
                +L+I   +   Q Q++E L   +I   K +++L NKIDL+ E       D++ + ++ 
Sbjct: 113190 AALLLIAGNEPCPQPQTSEHLAAVEIMKLKHIIILQNKIDLVKESDALNQFDEIKRFVKG 113369

Query: 170    TLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVD 229
              T+ +     +PIIP++A          +    I  + E +  +I IP RD +    + V 
Sbjct: 113370 TVAD----NSPIIPISA----------QLKYNIDVVCEHIVKRIPIPIRDFTSSPRLIVI 113507

Query: 230    HCFSIKG--------QGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITS 279
                F +          +G V  G+IL G + +GD +E+    +V K K   +   PITS
Sbjct: 113508 RSFDVNKPGEAVENLKGGVAGGSILRGVLRIGDEIEVRP-GIVTKEKDGTIKCKPITS 113678


>gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_69, whole genome
             shotgun sequence
          Length = 236320

 Score = 76.6 bits (187), Expect = 6e-14
 Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
 Frame = -2

Query: 1     MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
             M   + ++NV V+GH+D+GK+               R +      AA             
Sbjct: 70794 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 70615

Query: 37    DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
             DK    RERGIT+D+               +L +F++               T++D PGH
Sbjct: 70614 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 70510

Query: 97    ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
                I+ +I G    D+ +LV+D T G          Q+ E  L+   +  ++++V +NK 
Sbjct: 70509 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 70330

Query: 149   D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
             D   +    KR   I     +  K+L      G P     +P++   G     +++    
Sbjct: 70329 DDTTVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 70150

Query: 202   I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
                P L++ L + +  P R    P  + +   + I G GTV  G + +G +  G  V   
Sbjct: 70149 YKGPCLLDALDNLVE-PIRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMMVHFA 69973

Query: 259   PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
             P      +VKS++M H  +  A  GD +G  V       ++RG V +
Sbjct: 69972 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 69832


>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
              sequence
          Length = 1001995

 Score = 76.6 bits (187), Expect = 6e-14
 Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
 Frame = -1

Query: 1      MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
              M   + ++NV V+GH+D+GK+               R +      AA             
Sbjct: 892882 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 892703

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              DK    RERGIT+D+               +L +F++               T++D PGH
Sbjct: 892702 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 892598

Query: 97     ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
                 I+ +I G    D+ +LV+D T G          Q+ E  L+   +  ++++V +NK 
Sbjct: 892597 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 892418

Query: 149    D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
              D   +    KR   I     +  K+L      G P     +P++   G     +++    
Sbjct: 892417 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 892238

Query: 202    I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
                 P L++ L + +  P R    P  + +   + I G GTV  G + +G +  G  V   
Sbjct: 892237 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 892061

Query: 259    PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
              P      +VKS++M H  +  A  GD +G  V       ++RG V +
Sbjct: 892060 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 891920


>gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_25, whole genome shotgun
              sequence
          Length = 747259

 Score = 76.6 bits (187), Expect = 6e-14
 Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
 Frame = +2

Query: 1      MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
              M   + ++NV V+GH+D+GK+               R +      AA             
Sbjct: 181619 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 181798

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              DK    RERGIT+D+               +L +F++               T++D PGH
Sbjct: 181799 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 181903

Query: 97     ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
                 I+ +I G    D+ +LV+D T G          Q+ E  L+   +  ++++V +NK 
Sbjct: 181904 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 182083

Query: 149    D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
              D   +    KR   I     +  K+L      G P     +P++   G     +++    
Sbjct: 182084 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 182263

Query: 202    I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
                 P L++ L + +  P R    P  + +   + I G GTV  G + +G +  G  V   
Sbjct: 182264 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 182440

Query: 259    PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
              P      +VKS++M H  +  A  GD +G  V       ++RG V +
Sbjct: 182441 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 182581


>gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_5, whole genome shotgun
              sequence
          Length = 850158

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 80/339 (23%), Positives = 137/339 (40%), Gaps = 40/339 (11%)
 Frame = -1

Query: 8      VNVGVLGHIDSGKTALARAL------------------STTASTAAF------DKQPQSR 43
              +NV ++GH+D+GK+ L   L                  S     ++F      D+  + R
Sbjct: 518502 INVVIIGHVDAGKSTLMGHLLYKLGNVSENTLRKFKKESVEIGKSSFHFAWVMDEHEEER 518323

Query: 44     ERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTI 103
              +RG+T+D+G   F  P                            VT++D PGH   I  +
Sbjct: 518322 QRGVTMDVGVRYFETPNR-------------------------HVTILDAPGHKDFIPKM 518218

Query: 104    IGGAQIIDLMMLVIDVTK-GMQT------QSAECLVIGQ-IACQKLVVVLNKIDLLPEGK 155
              I GA   D  +LVID T  G +T      Q+ E L++ + +  ++++VV+NK+D +  G 
Sbjct: 518217 ITGAAQADFAILVIDSTPGGFETGFANGGQTKEHLILARSLGVEQVIVVVNKLDSI--GW 518044

Query: 156    RQAAIDKMTKKMQKTLENTKFR---GAPIIPVAAKPGGPEAPETEAPQGIPELIELLT-- 210
               +   D +  ++   +    F+   G+ +  + A   G +        G     + L+  
Sbjct: 518043 SKERYDSIVAQLDDFMRQIGFQTQDGSHVFYIPA--SGLQGENLITKSGSISWYDGLSVV 517870

Query: 211    ---SQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKV 267
                  +     RD      MSV   +     G  + G I +G+IS GD + I  ++ V  V
Sbjct: 517869 ERIDKFEPKPRDIEKALRMSVSDVYKSLATGITVAGKIETGTISEGDELVIMPIREVCTV 517690

Query: 268    KSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAP 306
              KS+     P+     GD + I +   D + L  G +  P
Sbjct: 517689 KSILRHKSPVKHGYAGDNVEIGLGSIDIEKLMVGQILCP 517573


>gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun
              sequence
          Length = 286411

 Score = 63.5 bits (153), Expect = 5e-10
 Identities = 78/330 (23%), Positives = 132/330 (40%), Gaps = 28/330 (8%)
 Frame = +3

Query: 8      VNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSS 67
              +NV  LGH+D+GK+ L+  +                + G+  D     F +      R S
Sbjct: 151050 LNVIFLGHVDAGKSTLSGRILL--------------DTGMIDDRVIEKFKMEAKENNRES 151187

Query: 68     --LPEFQAAPEAEPEPGEPL-----------LQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
                +       E E   G+ +            + T++D PGH + +  +I      D+ +
Sbjct: 151188 WYIAYIMDCSEDEKAKGKTIEVGRAHFETQKKRYTILDAPGHKAYVPNMISATTQADVGI 151367

Query: 115    LVIDVTKG-------MQTQSAECLVIGQ-IACQKLVVVLNKIDLLPEGKRQAAIDKMTKK 166
              LVI   KG          Q+ E  V+ + +   +L+VV+NKID     K +   D++  K
Sbjct: 151368 LVISARKGEFEAGLEKGGQTMEHTVLSKTLGVDQLIVVVNKIDDPSVAKDKVRYDEIVTK 151547

Query: 167    MQKTLENTKFR---GAPIIPVAAKPG-GPEAPETEAP-QGIPELIELLTSQISIPTRDPS 221
              +  TL+   +        IP+ A  G    +     P    P LIE L         D +
Sbjct: 151548 VSATLKGVGWNLKTQVQFIPIDALTGQNISSRNLNVPWYSGPSLIEALDDIKLTAVHDVN 151727

Query: 222    GPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAM 281
               P  + +   +  KG  TV+ G +LSG I +G  V +   K V +V +++   + I   +
Sbjct: 151728 APLRLPIIDRYRDKG-CTVVLGKVLSGQIKIGQKVVVMPNKCVCEVIALKSHEINIHGGV 151904

Query: 282    --QGDRLGICVTQFDPKLLERGLVCAPESL 309
                 G+ + + +   D  +     VC PE L
Sbjct: 151905 VSAGENVELYLKGDDDNIASGHTVCDPEHL 151994


>gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_19, whole genome shotgun
              sequence
          Length = 467654

 Score = 59.3 bits (142), Expect = 1e-08
 Identities = 60/249 (24%), Positives = 104/249 (41%), Gaps = 5/249 (2%)
 Frame = -3

Query: 10     VGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLP 69
              V ++GH+D GKT L   L    +T   DK+      GIT  +G                 
Sbjct: 215151 VTIMGHVDHGKTTL---LDKLRNTNLVDKE----FGGITQHIG----------------- 215044

Query: 70     EFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE 129
               FQ   E+  +P      +T +D PGH +       GAQ+ D+++LV+    G+  Q+ E
Sbjct: 215043 AFQVKLESVAKP------ITFIDTPGHEAFTSMRSCGAQVTDIIVLVVAADDGVMPQTRE 214882

Query: 130    CLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKM--QKTLENTKFRGAPIIPVAAK 187
               +   + A   ++V +NKID     K  A ++ + + +  +  +   K      IP++A 
Sbjct: 214881 VIKHAREANVPMIVAINKID-----KPNADVEMVERHLMNEGIVSEEKGGDTQFIPISAL 214717

Query: 188    PGGPEAPETEAPQGIPELIE---LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGT 244
               G          +G+ +LIE   L    + +     + P   +V  C  + G G   T  
Sbjct: 214716 KG----------KGLKDLIEAIGLQAEMLELKAMVDNVPMQGTVIECKVLHGFGISATCI 214567

Query: 245    ILSGSISLG 253
              +  G++ +G
Sbjct: 214566 VRRGALKVG 214540


>gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome
             shotgun sequence
          Length = 536960

 Score = 59.3 bits (142), Expect = 1e-08
 Identities = 66/280 (23%), Positives = 117/280 (41%), Gaps = 33/280 (11%)
 Frame = +1

Query: 12    VLGHIDSGKTALARALSTTASTAAFDKQPQSRERG-ITLDLGFSCFSVPLPARLRSSLPE 70
             VLGH+D GKT +   + +T          QS E G IT  +G +   VP+ A  + +   
Sbjct: 14347 VLGHVDVGKTKILDKIRST--------NVQSGEAGGITQQIGATY--VPIDAIKKQT--- 14487

Query: 71    FQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAEC 130
             F+     + +    L  + ++D PGH S       G+ + D+ +LVID+  G++ Q+ E 
Sbjct: 14488 FKLNESVKKQLQYNLPGLLIIDTPGHESFNNLRSRGSSLCDIAILVIDIKHGLERQTIES 14667

Query: 131   LVIGQIACQKLVVVLNKIDLLPEGKR--QAAIDKMTKKMQKTL----------------- 171
             L + +      +V LNK+D L E K    A I +  K  +K +                 
Sbjct: 14668 LQLIKNRKTPFIVALNKVDALYEWKATPNAPIRESLKNQKKNVIQQFEQKVKDVQLQLME 14847

Query: 172   ---------ENTKFRGA-PIIPVAAKPGGPEAPETEAPQGIPELIEL---LTSQISIPTR 218
                      +N  F+    ++P +A  G          +GIP+L+ L   LT +  +   
Sbjct: 14848 HGLNSELYYKNKDFKSCLSLVPTSAHTG----------EGIPDLLALMCQLTQKHMVKKL 14997

Query: 219   DPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI 258
                     ++     ++G GT +   + +G +  GD++ +
Sbjct: 14998 TYKSAIECTILEVKVVEGHGTTVDVILSNGILREGDTIVV 15117


>gb|GG738934.1| Naegleria gruberi genomic scaffold NAEGRscaffold_90, whole genome
             shotgun sequence
          Length = 97622

 Score = 49.7 bits (117), Expect = 8e-06
 Identities = 75/318 (23%), Positives = 130/318 (40%), Gaps = 32/318 (10%)
 Frame = -3

Query: 1     MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITL-DLGFSCFSVP 59
             M GR+    +G +G   SGKT+L R +        F K   S  + IT+ D+  S  S  
Sbjct: 55593 MDGRKPQYTLGFVGDHASGKTSLVRRM-------LFGKY-ISDPKSITINDISLSHLSAK 55438

Query: 60    LPARLRSSL-----PEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
                 + SS      P ++ +P       E L+       P   ++ R  I G    D ++
Sbjct: 55437 AITEITSSTTHNYDPFYRLSPTVHVYWNENLIFNLFDMIPTRGAVCRGWIKGVSFCDGVV 55258

Query: 115   LVIDVTKGM--QTQSAECLVIGQI---------ACQKLVVVLNKIDLLPEGKR-----QA 158
              +ID++K M    Q     +IG++           + L++V+NK+DL+          + 
Sbjct: 55257 FMIDISKFMVSNRQQVADEIIGKMLLGIFSTSSVVKDLIIVINKMDLVDNSLELFDFVRI 55078

Query: 159   AIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIP-ELIELLTS---QIS 214
              I+K  K+MQ T   T  +   IIP++             P G P E  +   S   ++ 
Sbjct: 55077 YIEKSFKQMQ-TFRKTTLK---IIPMSLM----NEKNIVRPFGSPFEQYDTFLSCLRELK 54922

Query: 215   IPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI------PALKVVKKVK 268
                 D + PF M     +S+ G G +  G ++SG +   +  EI         +   + +
Sbjct: 54921 PYPYDHTAPFRMIHFDTYSMYGLGRICAGKVISGMVRNTEEFEICQSTLRKTFQCTSQGQ 54742

Query: 269   SMQMFHMPITSAMQGDRL 286
             S+  F+  +T ++ G+ L
Sbjct: 54741 SLMSFYDSLTVSVAGELL 54688


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,426,717
Number of Sequences: 784
Number of extensions: 323408
Number of successful extensions: 1245
Number of sequences better than 1.0e-05: 11
Number of HSP's better than  0.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 1020
Number of HSP's gapped (non-prelim): 452
length of query: 596
length of database: 13,654,695
effective HSP length: 108
effective length of query: 488
effective length of database: 13,570,023
effective search space: 6622171224
effective search space used: 6622171224
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 116 (49.3 bits)