TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
(596 letters)
Database: N.gruberi/genome.fa
784 sequences; 40,964,085 total letters
Searching....................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 294 4e-83
gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 134 3e-31
gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 90 7e-18
gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 77 6e-14
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 77 6e-14
gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 77 6e-14
gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 76 1e-13
gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 64 5e-10
gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 59 1e-08
gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 59 1e-08
gb|GG738934.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 50 8e-06
>gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_11, whole genome
shotgun sequence
Length = 613270
Score = 294 bits (753), Expect(2) = 4e-83
Identities = 160/355 (45%), Positives = 220/355 (61%), Gaps = 30/355 (8%)
Frame = -1
Query: 12 VLGHIDSG--------------KTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFS 57
++GH+DSG KT+L +ALST ASTAAFDK PQS+ERGIT DLGFS F
Sbjct: 66928 IIGHVDSGEFLMKFILMIYLKGKTSLCKALSTHASTAAFDKSPQSKERGITQDLGFSAFY 66749
Query: 58 VPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVI 117
P L E+ +Q TLVDCPGHASLIRTIIGGAQIID M+LVI
Sbjct: 66748 SDSP------LSEYDR------------IQYTLVDCPGHASLIRTIIGGAQIIDFMVLVI 66623
Query: 118 DVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTK-----------K 166
D KG+QTQ+AEC+VIG+I+C L+V LNK+D++P +R+ +DK++ +
Sbjct: 66622 DAVKGVQTQTAECIVIGEISCNNLIVALNKVDMIPADQREDRVDKVSNFKIYLGN*FSLQ 66443
Query: 167 MQKTL-----ENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPS 221
++K+L TKF+ PI+ V A P E+ QG+ LI + I +D +
Sbjct: 66442 IKKSLIKNVFSQTKFKNPPIVSVCADPR-EESESITKEQGLKNLIAEFQKHVKISEKDEN 66266
Query: 222 GPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAM 281
PFL VDHCF+IKG+GT++TGT+L GS+S+G +++IP++ KKVKS+QMF P+ A+
Sbjct: 66265 EPFLYMVDHCFTIKGKGTIITGTVLQGSVSIGKTIQIPSIGEEKKVKSIQMFKTPVDKAI 66086
Query: 282 QGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFH 336
+GDR+GI VT+ D K LERG C + V L + +I +F+ ++ AK+H
Sbjct: 66085 KGDRIGIAVTKLDSKKLERGFACDKGYIEIVDTILANASRIRFFKKEIKNSAKYH 65921
Score = 66.6 bits (161), Expect = 6e-11
Identities = 38/100 (38%), Positives = 60/100 (60%), Gaps = 6/100 (6%)
Frame = -3
Query: 437 IHTNTCRLAFHGILLHGLEDRNYA-DSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFK 495
I N CRLAF+G ++ N + + + L V+K K K +V+R +D ++IG++L
Sbjct: 65615 IDANMCRLAFNGKVIKMWNKSNVSKEQLVNSLPVFKYKEKKAVVDRVVDANTIIGKNLLT 65436
Query: 496 KET-NIQLFVGLKVHLSTGEL----GIIDSAFGQSGKFKI 530
+T ++Q F+G K+ + + GIIDS FGQSGKFK+
Sbjct: 65435 SKTQSLQFFIGKKIDILIDDQPRFSGIIDSPFGQSGKFKV 65316
Score = 34.3 bits (77), Expect(2) = 4e-83
Identities = 33/122 (27%), Positives = 48/122 (39%), Gaps = 9/122 (7%)
Frame = -2
Query: 337 ITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADK 396
+++GHETVMG + + A I D+ + EY E+ +T+ +K
Sbjct: 65880 VSIGHETVMGNVSLIARAKKEGLSSNIFDT---TVEYSLLEE---------LTETTYQEK 65737
Query: 397 KAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADI---------HTNTCRLAFH 447
+ +EFEKPV C I S+LD DI +TN C
Sbjct: 65736 DV------------FVKIEFEKPVCCYPNSNYIASKLDTDISKFFTIIFSNTNRCEYV*I 65593
Query: 448 GI 449
GI
Sbjct: 65592 GI 65587
>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun
sequence
Length = 707703
Score = 134 bits (337), Expect = 3e-31
Identities = 98/320 (30%), Positives = 156/320 (48%), Gaps = 20/320 (6%)
Frame = -2
Query: 7 NVNVGVLGHIDSGKTALARALSTTASTA-----------AFDKQPQSRERGITLDLGFSC 55
++N+G +GH+D GKT L A++ + DK P+ ++RGIT+
Sbjct: 506453 HINIGTIGHVDHGKTTLTAAITKYLNELDPKANSFLAYDQIDKAPEEKQRGITIS----- 506289
Query: 56 FSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMML 115
+S E+ A +DCPGHA I+ +I GA +D +L
Sbjct: 506288 ----------TSHIEYATATR----------HYAHIDCPGHADYIKNMITGAAQMDGSIL 506169
Query: 116 VIDVTKGMQTQSAECLVI-GQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENT 174
VI G Q+ E L++ Q+ + +VV LNK+DL+P+ + I+ + ++++ T++
Sbjct: 506168 VISADDGPMLQTREHLLLCKQVGIKHIVVFLNKVDLVPDAEM---IEMVEEEIRDTVKQY 505998
Query: 175 KFRGAPIIPVAAKP-GGPEAPETE-APQGIPELIELLTSQISIPTRDPSGPFLMSVDHCF 232
F G I + G E +E Q I +L++ + I P RD + PFLM V+ F
Sbjct: 505997 GFDGDNISIIRGSALKGLEGDSSEIGTQAIQKLMDAVDKDIPEPERDINKPFLMPVEGVF 505818
Query: 233 SIKGQGTVMTGTILSGSISLGDSVEI-----PALKVVKKVKSMQMFHMPITSAMQGDRLG 287
+I G+GTV TG + G + LG VE+ ALK V ++MFH + GD LG
Sbjct: 505817 TISGRGTVATGKVDQGQLKLGAEVEVVGFAPTALK--SSVTGIEMFHKLVDRGQAGDNLG 505644
Query: 288 ICVTQFDPKLLERG-LVCAP 306
I + D K ++RG ++C P
Sbjct: 505643 ILLRGIDKKQIKRGQVICKP 505584
>gb|GG738872.1| Naegleria gruberi genomic scaffold NAEGRscaffold_28, whole genome shotgun
sequence
Length = 413107
Score = 89.7 bits (221), Expect = 7e-18
Identities = 83/298 (27%), Positives = 140/298 (46%), Gaps = 22/298 (7%)
Frame = +3
Query: 4 RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
R+ +N+G +GH+ GK+ + RA+S T F + R IT+ LG++ + L
Sbjct: 112842 RQATINIGTIGHVAHGKSTVVRAISGV-KTIRFKNE---MVRNITIKLGYANAKIFLCRD 113009
Query: 64 LRSSLPEFQAAP----------EAEPEPGEPLL--QVTLVDCPGHASLIRTIIGGAQIID 111
+ PE + E + GE L V+ VDCPGH L+ T++ GA ++D
Sbjct: 113010 PKCPRPECYTSQGSNHCDHFPCEVDDFKGEYELVRHVSFVDCPGHDILMATMLNGAAVMD 113189
Query: 112 LMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLPEGKRQAAIDKMTKKMQK 169
+L+I + Q Q++E L +I K +++L NKIDL+ E D++ + ++
Sbjct: 113190 AALLLIAGNEPCPQPQTSEHLAAVEIMKLKHIIILQNKIDLVKESDALNQFDEIKRFVKG 113369
Query: 170 TLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVD 229
T+ + +PIIP++A + I + E + +I IP RD + + V
Sbjct: 113370 TVAD----NSPIIPISA----------QLKYNIDVVCEHIVKRIPIPIRDFTSSPRLIVI 113507
Query: 230 HCFSIKG--------QGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITS 279
F + +G V G+IL G + +GD +E+ +V K K + PITS
Sbjct: 113508 RSFDVNKPGEAVENLKGGVAGGSILRGVLRIGDEIEVRP-GIVTKEKDGTIKCKPITS 113678
>gb|GG738913.1| Naegleria gruberi genomic scaffold NAEGRscaffold_69, whole genome
shotgun sequence
Length = 236320
Score = 76.6 bits (187), Expect = 6e-14
Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
Frame = -2
Query: 1 MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
M + ++NV V+GH+D+GK+ R + AA
Sbjct: 70794 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 70615
Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
DK RERGIT+D+ +L +F++ T++D PGH
Sbjct: 70614 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 70510
Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK
Sbjct: 70509 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 70330
Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
D + KR I + K+L G P +P++ G +++
Sbjct: 70329 DDTTVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 70150
Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
P L++ L + + P R P + + + I G GTV G + +G + G V
Sbjct: 70149 YKGPCLLDALDNLVE-PIRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMMVHFA 69973
Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
P +VKS++M H + A GD +G V ++RG V +
Sbjct: 69972 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 69832
>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
sequence
Length = 1001995
Score = 76.6 bits (187), Expect = 6e-14
Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
Frame = -1
Query: 1 MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
M + ++NV V+GH+D+GK+ R + AA
Sbjct: 892882 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 892703
Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
DK RERGIT+D+ +L +F++ T++D PGH
Sbjct: 892702 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 892598
Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK
Sbjct: 892597 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 892418
Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
D + KR I + K+L G P +P++ G +++
Sbjct: 892417 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 892238
Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
P L++ L + + P R P + + + I G GTV G + +G + G V
Sbjct: 892237 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 892061
Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
P +VKS++M H + A GD +G V ++RG V +
Sbjct: 892060 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 891920
>gb|GG738869.1| Naegleria gruberi genomic scaffold NAEGRscaffold_25, whole genome shotgun
sequence
Length = 747259
Score = 76.6 bits (187), Expect = 6e-14
Identities = 82/347 (23%), Positives = 138/347 (39%), Gaps = 42/347 (12%)
Frame = +2
Query: 1 MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
M + ++NV V+GH+D+GK+ R + AA
Sbjct: 181619 MGKEKSHINVVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKFEKEAAEMGKSSFKYAWVL 181798
Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
DK RERGIT+D+ +L +F++ T++D PGH
Sbjct: 181799 DKLKAERERGITIDI---------------ALWKFESKKYV----------FTIIDAPGH 181903
Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
I+ +I G D+ +LV+D T G Q+ E L+ + ++++V +NK
Sbjct: 181904 RDFIKNMITGTSQADVAILVVDSTNGGFEAGFSKDGQTREHALLAYTLGIKQMIVCMNKF 182083
Query: 149 D---LLPEGKRQAAIDKMTKKMQKTLENTKFRGAP----IIPVAAKPGGPEAPETEAPQG 201
D + KR I + K+L G P +P++ G +++
Sbjct: 182084 DDESVKYSDKRYEEIKSEVGRYLKSLGFQTEEGKPNCVQFVPISGWTGDNMIEKSDKMPW 182263
Query: 202 I--PELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI- 258
P L++ L + + P R P + + + I G GTV G + +G + G V
Sbjct: 182264 YKGPCLLDALDNLVE-PVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGKLKPGMVVHFA 182440
Query: 259 PALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
P +VKS++M H + A GD +G V ++RG V +
Sbjct: 182441 PGQAADTEVKSVEMHHTSVPEAGPGDNVGFNVKGLSTTDIKRGYVAS 182581
>gb|GG738849.1| Naegleria gruberi genomic scaffold NAEGRscaffold_5, whole genome shotgun
sequence
Length = 850158
Score = 75.9 bits (185), Expect = 1e-13
Identities = 80/339 (23%), Positives = 137/339 (40%), Gaps = 40/339 (11%)
Frame = -1
Query: 8 VNVGVLGHIDSGKTALARAL------------------STTASTAAF------DKQPQSR 43
+NV ++GH+D+GK+ L L S ++F D+ + R
Sbjct: 518502 INVVIIGHVDAGKSTLMGHLLYKLGNVSENTLRKFKKESVEIGKSSFHFAWVMDEHEEER 518323
Query: 44 ERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTI 103
+RG+T+D+G F P VT++D PGH I +
Sbjct: 518322 QRGVTMDVGVRYFETPNR-------------------------HVTILDAPGHKDFIPKM 518218
Query: 104 IGGAQIIDLMMLVIDVTK-GMQT------QSAECLVIGQ-IACQKLVVVLNKIDLLPEGK 155
I GA D +LVID T G +T Q+ E L++ + + ++++VV+NK+D + G
Sbjct: 518217 ITGAAQADFAILVIDSTPGGFETGFANGGQTKEHLILARSLGVEQVIVVVNKLDSI--GW 518044
Query: 156 RQAAIDKMTKKMQKTLENTKFR---GAPIIPVAAKPGGPEAPETEAPQGIPELIELLT-- 210
+ D + ++ + F+ G+ + + A G + G + L+
Sbjct: 518043 SKERYDSIVAQLDDFMRQIGFQTQDGSHVFYIPA--SGLQGENLITKSGSISWYDGLSVV 517870
Query: 211 ---SQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKV 267
+ RD MSV + G + G I +G+IS GD + I ++ V V
Sbjct: 517869 ERIDKFEPKPRDIEKALRMSVSDVYKSLATGITVAGKIETGTISEGDELVIMPIREVCTV 517690
Query: 268 KSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAP 306
KS+ P+ GD + I + D + L G + P
Sbjct: 517689 KSILRHKSPVKHGYAGDNVEIGLGSIDIEKLMVGQILCP 517573
>gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun
sequence
Length = 286411
Score = 63.5 bits (153), Expect = 5e-10
Identities = 78/330 (23%), Positives = 132/330 (40%), Gaps = 28/330 (8%)
Frame = +3
Query: 8 VNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSS 67
+NV LGH+D+GK+ L+ + + G+ D F + R S
Sbjct: 151050 LNVIFLGHVDAGKSTLSGRILL--------------DTGMIDDRVIEKFKMEAKENNRES 151187
Query: 68 --LPEFQAAPEAEPEPGEPL-----------LQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
+ E E G+ + + T++D PGH + + +I D+ +
Sbjct: 151188 WYIAYIMDCSEDEKAKGKTIEVGRAHFETQKKRYTILDAPGHKAYVPNMISATTQADVGI 151367
Query: 115 LVIDVTKG-------MQTQSAECLVIGQ-IACQKLVVVLNKIDLLPEGKRQAAIDKMTKK 166
LVI KG Q+ E V+ + + +L+VV+NKID K + D++ K
Sbjct: 151368 LVISARKGEFEAGLEKGGQTMEHTVLSKTLGVDQLIVVVNKIDDPSVAKDKVRYDEIVTK 151547
Query: 167 MQKTLENTKFR---GAPIIPVAAKPG-GPEAPETEAP-QGIPELIELLTSQISIPTRDPS 221
+ TL+ + IP+ A G + P P LIE L D +
Sbjct: 151548 VSATLKGVGWNLKTQVQFIPIDALTGQNISSRNLNVPWYSGPSLIEALDDIKLTAVHDVN 151727
Query: 222 GPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAM 281
P + + + KG TV+ G +LSG I +G V + K V +V +++ + I +
Sbjct: 151728 APLRLPIIDRYRDKG-CTVVLGKVLSGQIKIGQKVVVMPNKCVCEVIALKSHEINIHGGV 151904
Query: 282 --QGDRLGICVTQFDPKLLERGLVCAPESL 309
G+ + + + D + VC PE L
Sbjct: 151905 VSAGENVELYLKGDDDNIASGHTVCDPEHL 151994
>gb|GG738863.1| Naegleria gruberi genomic scaffold NAEGRscaffold_19, whole genome shotgun
sequence
Length = 467654
Score = 59.3 bits (142), Expect = 1e-08
Identities = 60/249 (24%), Positives = 104/249 (41%), Gaps = 5/249 (2%)
Frame = -3
Query: 10 VGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLP 69
V ++GH+D GKT L L +T DK+ GIT +G
Sbjct: 215151 VTIMGHVDHGKTTL---LDKLRNTNLVDKE----FGGITQHIG----------------- 215044
Query: 70 EFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE 129
FQ E+ +P +T +D PGH + GAQ+ D+++LV+ G+ Q+ E
Sbjct: 215043 AFQVKLESVAKP------ITFIDTPGHEAFTSMRSCGAQVTDIIVLVVAADDGVMPQTRE 214882
Query: 130 CLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKM--QKTLENTKFRGAPIIPVAAK 187
+ + A ++V +NKID K A ++ + + + + + K IP++A
Sbjct: 214881 VIKHAREANVPMIVAINKID-----KPNADVEMVERHLMNEGIVSEEKGGDTQFIPISAL 214717
Query: 188 PGGPEAPETEAPQGIPELIE---LLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGT 244
G +G+ +LIE L + + + P +V C + G G T
Sbjct: 214716 KG----------KGLKDLIEAIGLQAEMLELKAMVDNVPMQGTVIECKVLHGFGISATCI 214567
Query: 245 ILSGSISLG 253
+ G++ +G
Sbjct: 214566 VRRGALKVG 214540
>gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome
shotgun sequence
Length = 536960
Score = 59.3 bits (142), Expect = 1e-08
Identities = 66/280 (23%), Positives = 117/280 (41%), Gaps = 33/280 (11%)
Frame = +1
Query: 12 VLGHIDSGKTALARALSTTASTAAFDKQPQSRERG-ITLDLGFSCFSVPLPARLRSSLPE 70
VLGH+D GKT + + +T QS E G IT +G + VP+ A + +
Sbjct: 14347 VLGHVDVGKTKILDKIRST--------NVQSGEAGGITQQIGATY--VPIDAIKKQT--- 14487
Query: 71 FQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAEC 130
F+ + + L + ++D PGH S G+ + D+ +LVID+ G++ Q+ E
Sbjct: 14488 FKLNESVKKQLQYNLPGLLIIDTPGHESFNNLRSRGSSLCDIAILVIDIKHGLERQTIES 14667
Query: 131 LVIGQIACQKLVVVLNKIDLLPEGKR--QAAIDKMTKKMQKTL----------------- 171
L + + +V LNK+D L E K A I + K +K +
Sbjct: 14668 LQLIKNRKTPFIVALNKVDALYEWKATPNAPIRESLKNQKKNVIQQFEQKVKDVQLQLME 14847
Query: 172 ---------ENTKFRGA-PIIPVAAKPGGPEAPETEAPQGIPELIEL---LTSQISIPTR 218
+N F+ ++P +A G +GIP+L+ L LT + +
Sbjct: 14848 HGLNSELYYKNKDFKSCLSLVPTSAHTG----------EGIPDLLALMCQLTQKHMVKKL 14997
Query: 219 DPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI 258
++ ++G GT + + +G + GD++ +
Sbjct: 14998 TYKSAIECTILEVKVVEGHGTTVDVILSNGILREGDTIVV 15117
>gb|GG738934.1| Naegleria gruberi genomic scaffold NAEGRscaffold_90, whole genome
shotgun sequence
Length = 97622
Score = 49.7 bits (117), Expect = 8e-06
Identities = 75/318 (23%), Positives = 130/318 (40%), Gaps = 32/318 (10%)
Frame = -3
Query: 1 MAGRRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITL-DLGFSCFSVP 59
M GR+ +G +G SGKT+L R + F K S + IT+ D+ S S
Sbjct: 55593 MDGRKPQYTLGFVGDHASGKTSLVRRM-------LFGKY-ISDPKSITINDISLSHLSAK 55438
Query: 60 LPARLRSSL-----PEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
+ SS P ++ +P E L+ P ++ R I G D ++
Sbjct: 55437 AITEITSSTTHNYDPFYRLSPTVHVYWNENLIFNLFDMIPTRGAVCRGWIKGVSFCDGVV 55258
Query: 115 LVIDVTKGM--QTQSAECLVIGQI---------ACQKLVVVLNKIDLLPEGKR-----QA 158
+ID++K M Q +IG++ + L++V+NK+DL+ +
Sbjct: 55257 FMIDISKFMVSNRQQVADEIIGKMLLGIFSTSSVVKDLIIVINKMDLVDNSLELFDFVRI 55078
Query: 159 AIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIP-ELIELLTS---QIS 214
I+K K+MQ T T + IIP++ P G P E + S ++
Sbjct: 55077 YIEKSFKQMQ-TFRKTTLK---IIPMSLM----NEKNIVRPFGSPFEQYDTFLSCLRELK 54922
Query: 215 IPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI------PALKVVKKVK 268
D + PF M +S+ G G + G ++SG + + EI + + +
Sbjct: 54921 PYPYDHTAPFRMIHFDTYSMYGLGRICAGKVISGMVRNTEEFEICQSTLRKTFQCTSQGQ 54742
Query: 269 SMQMFHMPITSAMQGDRL 286
S+ F+ +T ++ G+ L
Sbjct: 54741 SLMSFYDSLTVSVAGELL 54688
Database: N.gruberi/genome.fa
Posted date: Feb 9, 2011 9:02 AM
Number of letters in database: 40,964,085
Number of sequences in database: 784
Lambda K H
0.319 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,426,717
Number of Sequences: 784
Number of extensions: 323408
Number of successful extensions: 1245
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 1020
Number of HSP's gapped (non-prelim): 452
length of query: 596
length of database: 13,654,695
effective HSP length: 108
effective length of query: 488
effective length of database: 13,570,023
effective search space: 6622171224
effective search space used: 6622171224
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 116 (49.3 bits)