TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: C.parvum/genome.fa 
           8 sequences; 9,102,324 total letters

Searching........done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CM000431.1| Cryptosporidium parvum Iowa II chromosome 3, whol...    98   6e-21
gb|CM000434.1| Cryptosporidium parvum Iowa II chromosome 6, whol...    82   3e-16
gb|CM000430.1| Cryptosporidium parvum Iowa II chromosome 2, whol...    53   2e-07

>gb|CM000431.1| Cryptosporidium parvum Iowa II chromosome 3, whole genome shotgun
              sequence
          Length = 1099352

 Score = 97.8 bits (242), Expect = 6e-21
 Identities = 87/329 (26%), Positives = 153/329 (46%), Gaps = 35/329 (10%)
 Frame = -2

Query: 4      RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSV----- 58
              R+  +N+G +GH+  GK+ + RA+S   +    D+    +ER IT+ LG++   +     
Sbjct: 451192 RQATINLGTIGHVAHGKSTVVRAVSGVQTVRFKDE----KERNITIKLGYANAKIYKCTN 451025

Query: 59     ---PLPARLRSSLPEFQAAPEAEPEPGEPLLQ----VTLVDCPGHASLIRTIIGGAQIID 111
                 P P   RS     +  P  E       +Q    V+ VDCPGH  L+ T++ GA ++D
Sbjct: 451024 PNCPPPKCYRSYGSNKEDEPMCEVPSCNHKMQLLRHVSFVDCPGHDILMSTMLNGAAVMD 450845

Query: 112    LMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLPEGKRQAAIDKMTKKMQK 169
                +L++   +   Q Q++E L   +I   K +++L NK++L+ E + Q    +  K+++ 
Sbjct: 450844 AALLLVAGNETCPQPQTSEHLAAVEIMKLKHIIILQNKVELIKEAQAQ----EQYKQIKD 450677

Query: 170    TLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVD 229
               +  T  + APIIP++A               I  L E + +QI IP RD +    M + 
Sbjct: 450676 FVAGTSAQDAPIIPISA----------VLKFNIDVLCEYICTQIPIPIRDFTSSPRMIII 450527

Query: 230    HCFSIKG--------QGTVMTGTILSGSISLGDSVEI-PAL------------KVVKKVK 268
                F +          +G V  G+I+ G + +GD +E+ P +             +  K+ 
Sbjct: 450526 RSFDVNKPGEDAQNMRGGVAGGSIIKGVLKIGDEIEVRPGIYNKDADGEFTCRPIRSKIV 450347

Query: 269    SMQMFHMPITSAMQGDRLGICVTQFDPKL 297
              S+   H  +  A+ G  +G+  T+ DP L
Sbjct: 450346 SLFAEHNDLKYAVSGGLIGV-GTKIDPTL 450263


>gb|CM000434.1| Cryptosporidium parvum Iowa II chromosome 6, whole genome shotgun
              sequence
          Length = 1332857

 Score = 82.4 bits (202), Expect = 3e-16
 Identities = 81/340 (23%), Positives = 135/340 (39%), Gaps = 35/340 (10%)
 Frame = -3

Query: 1      MAGRRVNVNVGVLGHIDSGKTALARAL------------------STTASTAAF------ 36
              M   + ++N+ V+GH+DSGK+     L                  S+     +F      
Sbjct: 929115 MGKEKTHINLVVIGHVDSGKSTTTGHLIYKLGGIDKRTIEKFEKESSEMGKGSFKYAWVL 928936

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              DK    RERGIT+D+    F  P                             T++D PGH
Sbjct: 928935 DKLKAERERGITIDIALWQFETPK-------------------------YHYTVIDAPGH 928831

Query: 97     ASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE------CLVIGQIACQKLVVVLNKIDL 150
                 I+ +I G    D+ +LV+   +     S E       L+   +  ++++V +NK+D 
Sbjct: 928830 RDFIKNMITGTSQADVALLVVPADRFEGAFSKEGQTREHALLAFTLGVRQMIVGINKMDT 928651

Query: 151    LPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIP-----EL 205
                   +Q+  D++  ++   L+   +    I P  A  G       E    +P      L
Sbjct: 928650 CEY--KQSRFDEIFNEVDGYLKKVGYNTEKI-PFVAISGFVGDNMVERSDKMPWYKGKTL 928480

Query: 206    IELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVK 265
              +E L + +  P R    P  + +   + I G GTV  G + +G I  G +V      V  
Sbjct: 928479 VEALDT-MEPPKRPTDKPLRLPLQDVYKIGGVGTVPVGRVETGIIRPGMNVTFAPAGVTT 928303

Query: 266    KVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
              +VKS++M H  +  A+ GD +G  V     K ++RG V +
Sbjct: 928302 EVKSVEMHHEQMPEAVPGDNVGFNVKNVSIKDIKRGFVAS 928183



 Score = 58.5 bits (140), Expect = 4e-09
 Identities = 68/306 (22%), Positives = 125/306 (40%), Gaps = 43/306 (14%)
 Frame = +2

Query: 12     VLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEF 71
              +LGH+D+GKT L   +  T           +   GIT  +G + F    P  L   + + 
Sbjct: 519359 ILGHVDTGKTKLLDKMRKT-------NVQDNEAGGITQQIGATYFP---PEMLSEQVKKV 519508

Query: 72     QAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL 131
              +A  E +  PG     +  +D PGH S       G+ + D+ +LV+D+  G++ Q+ E +
Sbjct: 519509 EADFELQI-PG-----LLFIDTPGHESFNNLRSRGSSLCDIAVLVVDIMHGLEPQTRESI 519670

Query: 132    VIGQIACQKLVVVLNKIDLL--------------PEGKRQAAIDKMTKKMQKTL------ 171
               + +      ++ LNKID L                 + ++  D+   ++ + L      
Sbjct: 519671 GLLRSRKCPFIIALNKIDRLYGWIEQNWSSSRSTLSIQNESTRDEFDTRLNRVLLELSEE 519850

Query: 172    --------ENTKFRG-APIIPVAAKPGGPEAPETEAPQGIPELIELLTSQ---------I 213
                      +N  FRG   I+P +A  G          +G+P+LI L+            +
Sbjct: 519851 GLNCDIYWKNDDFRGNVSIVPTSAVTG----------EGVPDLIYLIAQLTQNYMGLHCL 520000

Query: 214    SIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEI-----PALKVVKKVK 268
               + TR+ S   L       +I G G  +   ++SG +  GD++ +     P +  ++ + 
Sbjct: 520001 QLNTRELSCTIL----EVKAIDGLGVTIDVILVSGILREGDTIIVCGLSAPIVTTIRALL 520168

Query: 269    SMQMFH 274
              + Q  H
Sbjct: 520169 TPQPMH 520186



 Score = 51.6 bits (122), Expect = 5e-07
 Identities = 43/152 (28%), Positives = 72/152 (47%), Gaps = 9/152 (5%)
 Frame = +3

Query: 9      NVGVLGHIDSGKTALARAL--------STTASTAAF-DKQPQSRERGITLDLGFSCFSVP 59
              NV ++ H+D GKT LA  L        + +A T  + D +   + R IT+       +V 
Sbjct: 922386 NVCIIAHVDHGKTTLADYLLASNNILSNKSAGTIRYLDSREDEQYRLITMKSS----AVS 922553

Query: 60     LPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDV 119
              L  +    +       + E E G+ L  + L+D PGH      +I   +I D  +L++DV
Sbjct: 922554 LKFKYEEEI-------KLEVEDGDYL--INLIDSPGHVDFTYEVISSLRISDGALLLVDV 922706

Query: 120    TKGMQTQSAECLVIGQIACQKLVVVLNKIDLL 151
               +G+  Q+ + L        K+++VLNK+D L
Sbjct: 922707 AEGIGDQTRKVLQHAFKERLKIILVLNKMDRL 922802


>gb|CM000430.1| Cryptosporidium parvum Iowa II chromosome 2, whole genome shotgun
              sequence
          Length = 985969

 Score = 53.1 bits (126), Expect = 2e-07
 Identities = 67/340 (19%), Positives = 133/340 (39%), Gaps = 36/340 (10%)
 Frame = -3

Query: 5      RVNVNVGVLGHIDSGKTALARALSTTASTA------AFDKQPQSRERGITLDLGFSCFSV 58
              R ++N+  +GH+D+GK+     +  T           ++++ + + R    +  F  F +
Sbjct: 419654 RPHMNIVFIGHVDAGKSTTCGNILYTCGLVDQRTMEKYEREAKEKNR----ESWFLAFIM 419487

Query: 59     PLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVID 118
                    RS     +    A         + T++D PGH S +  +I GA   D+ +L+I 
Sbjct: 419486 DTNEEERSKGKTVEVGRAAFNLKNR---RFTILDAPGHKSFVPNMISGASQADIGVLIIS 419316

Query: 119    VTKGM---------QTQSAECLVIGQIACQKLVVVLNKID---LLPEGKRQAAIDKMTKK 166
                KG          QT+    ++   +   +L+V +NK+D    + +  R   I+K    
Sbjct: 419315 ARKGEFETGFERGGQTRE-HAMLAKTLGVNQLIVAINKMDDPTCMWDQNRYNEIEKKLTP 419139

Query: 167    MQKTLENTKFRGAPIIPVAAKPG-----------GPEAPETEA---PQGIPELIELLTSQ 212
                KT      +    +P++ + G            P+  +  A       P L ++L   
Sbjct: 419138 YLKTCGYNPAKDIFFVPISGQLGQNLKYHVSDQSNPKYFDHRASWYSMEKPTLFDILDKY 418959

Query: 213    ISIPTRDPSGP--FLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSM 270
              +S+P R        ++ +      +  G +  G I  G+I  GD++ +   +   K++S+
Sbjct: 418958 LSVPDRSSGNEDNGIIRIPLLDGYRDNGVIAMGKIELGTIRCGDNLVVMPNRAKAKIQSI 418779

Query: 271    QMFHMPITSAMQ--GDRLGICVTQFDPKLLERGLVCAPES 308
               +       A    G+ + I +   D   L +G V   +S
Sbjct: 418778 CLGEEMDEYAWSGPGENVRIKLLNIDEDSLSKGFVLCSQS 418659


  Database: C.parvum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 9,102,324
  Number of sequences in database:  8
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,772,250
Number of Sequences: 8
Number of extensions: 62505
Number of successful extensions: 265
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 215
Number of HSP's gapped (non-prelim): 136
length of query: 596
length of database: 3,034,108
effective HSP length: 97
effective length of query: 499
effective length of database: 3,033,332
effective search space: 1513632668
effective search space used: 1513632668
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)