TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
         (596 letters)

Database: C.muris/genome.fa 
           84 sequences; 9,245,251 total letters

Searching...................................................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 geno...    91   6e-19
gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 geno...    83   2e-16
gb|DS989729.1| Cryptosporidium muris RN66 scf_1106632373431 geno...    54   1e-07

>gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 genomic scaffold, whole genome
               shotgun sequence
          Length = 1324930

 Score = 91.3 bits (225), Expect = 6e-19
 Identities = 87/333 (26%), Positives = 157/333 (47%), Gaps = 39/333 (11%)
 Frame = -1

Query: 4       RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSV----- 58
               R+  +N+G +GH+  GK+ + RA+S   +    D+    +ER IT+ LG++   +     
Sbjct: 1322071 RQATINLGTIGHVAHGKSTVVRAISGVQTVRFKDE----KERNITIKLGYANAKIYKCSN 1321904

Query: 59      ---PLPARLRSSLPEFQAAPEAEP--------EPGEPLLQVTLVDCPGHASLIRTIIGGA 107
                  P P   RS    + ++ E EP           + +  V+ VDCPGH  L+ T++ GA
Sbjct: 1321903 PDCPPPQCYRS----YGSSKEDEPICEVSGCNHKMKLMRHVSFVDCPGHDILMSTMLNGA 1321736

Query: 108     QIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLPEGKRQAAIDKMTK 165
                ++D  +L++   +   Q Q++E L   +I   + +++L NK++L+ E +   AI++  K
Sbjct: 1321735 AVMDAALLLVAGNESCPQPQTSEHLAAVEIMKLRHIIILQNKVELIKEAQ---AIEQ-HK 1321568

Query: 166     KMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFL 225
               +++  +  T  + A IIP++A               I  L E + +QI IP RD +    
Sbjct: 1321567 QIKDFVAGTSAQDAAIIPISA----------VLKFNIDVLCEYICTQIPIPIRDFTSSPR 1321418

Query: 226     MSVDHCFSIKG--------QGTVMTGTILSGSISLGDSVEI-PAL------------KVV 264
               M V   F +          +G V  G+I+ G + LGD +E+ P +             + 
Sbjct: 1321417 MIVIRSFDVNKPGEDAKSMRGGVAGGSIIRGVLKLGDEIEVRPGIYNKNSEGAFVCRPIR 1321238

Query: 265     KKVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297
                ++ S+   H  +  A+ G  +G+  T+ DP L
Sbjct: 1321237 SRIVSLFAEHNDLQYAVSGGLIGV-GTKIDPTL 1321142


>gb|DS989728.1| Cryptosporidium muris RN66 scf_1106632373407 genomic scaffold, whole
              genome shotgun sequence
          Length = 965821

 Score = 83.2 bits (204), Expect = 2e-16
 Identities = 80/340 (23%), Positives = 138/340 (40%), Gaps = 35/340 (10%)
 Frame = +2

Query: 1      MAGRRVNVNVGVLGHIDSGKTALARAL------------------STTASTAAF------ 36
              M   + ++N+ V+GH+DSGK+     L                  S+     +F      
Sbjct: 137657 MGKEKTHINLVVIGHVDSGKSTTTGHLIYKLGGIDKRTIEKFEKESSEMGKGSFKYAWVL 137836

Query: 37     DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
              DK    RERGIT+D+    F  P                           + T++D PGH
Sbjct: 137837 DKLKAERERGITIDIALWKFETPR-------------------------YEYTVIDAPGH 137941

Query: 97     ASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAE------CLVIGQIACQKLVVVLNKIDL 150
                 I+ +I G    D+ +LV+   +     S E       L+   +  ++++V +NK+D 
Sbjct: 137942 RDFIKNMITGTSQADVALLVVPADRFEGAFSKEGQTREHALLAFTLGVKQMIVGINKMDT 138121

Query: 151    LPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIP-----EL 205
                   +Q+  D++  +++  L+   +     IP  A  G       E    +P      L
Sbjct: 138122 CDY--KQSRYDEIHNEVEGYLKKVGYN-IEKIPFVAISGFVGDNMVEKSDKMPWYKGRTL 138292

Query: 206    IELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVK 265
              +E L + +  P R    P  + +   + I G GTV  G + +G I  G +V    + +  
Sbjct: 138293 VEALDT-MEPPKRPTEKPLRLPLQDVYKIGGVGTVPVGRVETGIIKPGMNVTFAPVGITT 138469

Query: 266    KVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
              +VKS++M H  ++ A  GD +G  V     K ++RG V +
Sbjct: 138470 EVKSVEMHHEQLSEAGPGDNVGFNVKNVSIKDIKRGYVAS 138589



 Score = 47.4 bits (111), Expect = 9e-06
 Identities = 40/152 (26%), Positives = 69/152 (45%), Gaps = 9/152 (5%)
 Frame = -1

Query: 9      NVGVLGHIDSGKTALARAL--------STTASTAAF-DKQPQSRERGITLDLGFSCFSVP 59
              NV ++ H+D GKT LA  L        S TA T  + D +   + R IT+    S  S+ 
Sbjct: 144004 NVCIIAHVDHGKTTLADFLLASNNILSSRTAGTMRYLDSREDEQYRLITMKS--SAISLT 143831

Query: 60     LPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDV 119
                    + +  F    +        L  + L+D PGH      +    ++ D  ++++DV
Sbjct: 143830 Y-----NYIDPFLNVSDL-------LYTINLIDSPGHVDFAHEVSSALRLCDGALILVDV 143687

Query: 120    TKGMQTQSAECLVIGQIACQKLVVVLNKIDLL 151
               +G+  Q+ + L    I   ++++VLNK+D L
Sbjct: 143686 VEGLGDQTRKVLQQAYIERVQMLLVLNKMDRL 143591


>gb|DS989729.1| Cryptosporidium muris RN66 scf_1106632373431 genomic scaffold, whole
              genome shotgun sequence
          Length = 772423

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 74/351 (21%), Positives = 138/351 (39%), Gaps = 54/351 (15%)
 Frame = -2

Query: 12     VLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEF 71
              +LGH+D+GKT +   +  T           +   GIT  +G + F    P  L   +   
Sbjct: 152463 ILGHVDTGKTKILDKMRKT-------NIQDNEAGGITQQIGATFFP---PEMLSEQIKRV 152314

Query: 72     QAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL 131
                  E +  PG     +  +D PGH S       G+ + D+ +LV+D+  G++ Q+ E +
Sbjct: 152313 DREFELQI-PG-----LLFIDTPGHESFNNLRSRGSSLCDIAVLVVDIMHGLEPQTKESI 152152

Query: 132    VIGQIACQKLVVVLNKIDLL--------------PEGKRQAAIDKMTKKMQKTL------ 171
               + +      ++ LNKID L               + ++    D+   ++ +TL      
Sbjct: 152151 GLLRNRKCPFIIALNKIDRLYGWTGCSWLPIRDTLDKQKSYVNDEFGTRLSQTLLQLSEE 151972

Query: 172    --------ENTKFRG-APIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSG 222
                      EN   R    I+P +A  G          +G+P+LI L+       T+   G
Sbjct: 151971 GLNCCPYWENDDLRSTVSIVPTSAITG----------EGVPDLIYLIAKL----TQKIMG 151834

Query: 223    PFLMSVD---------HCFSIKGQGTVMTGTILSGSISLGDSVEI-----PALKVVK--- 265
                 + ++            +I G G  +   ++SG++  GD++ +     P +  ++   
Sbjct: 151833 LNFLKLNKTELSCTILEVKAIDGLGVTIDVILVSGTLHEGDTIVVCGLSAPIVTTIRALL 151654

Query: 266    --------KVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPES 308
                      +VKS  + H  IT++M    + IC    D  +    L+  P++
Sbjct: 151653 TPQPMRELRVKSDYVHHHSITASM---GVKICANGLDDAVAGTQLMIVPKN 151510


  Database: C.muris/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 9,245,251
  Number of sequences in database:  84
  
Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,648,870
Number of Sequences: 84
Number of extensions: 58552
Number of successful extensions: 216
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 171
Number of HSP's gapped (non-prelim): 113
length of query: 596
length of database: 3,081,750
effective HSP length: 97
effective length of query: 499
effective length of database: 3,073,602
effective search space: 1533727398
effective search space used: 1533727398
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 111 (47.4 bits)