TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|320165142|gb|EFW42041.1| sec tRNA synthase [Capsaspora
owczarzaki ATCC 30864]
(470 letters)
Database: S.parasitica/genome.fa
1445 sequences; 53,132,636 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|GG743892.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 184 3e-99
>gb|GG743892.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.10, whole
genome shotgun sequence
Length = 790954
Score = 184 bits (468), Expect(3) = 3e-99
Identities = 119/289 (41%), Positives = 165/289 (57%), Gaps = 71/289 (24%)
Frame = +1
Query: 186 GFEVVVVDNAIEGDEVRTDLSALQHQVNTVGKDNILCIVSTTSCFAPRCPDRIVEIAKIC 245
G E VV++N ++GDE+RTDL+AL+ ++ +G +NILC++STTSCFAPR DR+ EIA++C
Sbjct: 241720 GLEPVVIENVLDGDELRTDLAALEAKMRELGPENILCVLSTTSCFAPRGYDRVDEIAQLC 241899
Query: 246 ------ATES-----------IPHVINNAYGIQSSKITALIQD----------------- 271
AT S + HVINNAYG+QSSK T L+
Sbjct: 241900 RQYDIGATSSPLAPCSICIVQLGHVINNAYGLQSSKCTHLVNQVRIY*TLENDDEARVA* 242079
Query: 272 AHKAGRVDAFVQSSDKNLMVPVGGALVA------GHDRAFIKRIGEMYP----------- 314
A + GRVDA +QS+DKN +VPVGGA+++ DR + R +P
Sbjct: 242080 AFRLGRVDACIQSTDKNFLVPVGGAVISVTSGLCTSDRQDVPRSIASHPPPPPWPCVMTL 242259
Query: 315 --GRASAAPSVDIFITLLSMGQQGFRSLLKQRKVVFEQLRAKLGAFAVSQGERLLETPHN 372
GRAS AP++D+FIT+L +G++G+++LL +RK + A L + A + GER+L T HN
Sbjct: 242260 RVGRASNAPALDLFITMLHLGRRGYKALLDERKRLVPYFSAALTSVATAHGERVLVTRHN 242439
Query: 373 P-----------------ISLAITLS-TFGSDATRLGSFLFTRCVSGTR 403
IS +TLS G+DAT LGS LF+R VSGTR
Sbjct: 242440 DVHIVDTSVPSVRPNLV*ISFCLTLSHVGGNDATFLGSMLFSRGVSGTR 242586
Score = 182 bits (463), Expect(3) = 3e-99
Identities = 101/192 (52%), Positives = 134/192 (69%), Gaps = 19/192 (9%)
Frame = +3
Query: 1 MDTEINQNLAKDILGNDAYIQQGADAIRTRQNLVKSLLAQRKWPELGWDEMSIELLLSDL 60
M + +NQ LA D + + AYI+QG+DA+++ + + +LLAQRK PE GWD++SI++LL+DL
Sbjct: 241065 MQSSVNQKLATDFV-DAAYIRQGSDALKSHEKKLTALLAQRKLPEDGWDDLSIQVLLNDL 241241
Query: 61 AQMDSNNFPANIGAGEREARVYSDLVARRHFRMAHGIGRSGDIAAIQPKAAGSSLIMKLV 120
AQMDSNNF N GAGEREARV S LV R F +AHG+GRSGDI A+QPKAAGSSL+++L
Sbjct: 241242 AQMDSNNFAHNAGAGEREARVASSLVRNRCFHLAHGVGRSGDICAVQPKAAGSSLLVQLT 241421
Query: 121 NIMCLDVIRLA--------------GVHAANACIALPLATGMAMVMTLLTL-AQT----R 161
N + LD++ LA G+ A + I LP+ATGMA++ +LTL AQ R
Sbjct: 241422 NCLVLDMLHLAGRRRIPCSDFIV*LGIKNAKSAIVLPVATGMALLFAMLTLQAQARVPGR 241601
Query: 162 PPSARKVLWLRI 173
P + R VLW RI
Sbjct: 241602 PHTKRYVLWPRI 241637
Score = 36.2 bits (82), Expect(3) = 3e-99
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +3
Query: 420 SLSNFGAHHSAYHGAYLTAAAAIGMTEQEVDAL 452
+ FGAH + Y AYL+AA AIGMT+ D++
Sbjct: 242673 AFEGFGAHSATYPTAYLSAACAIGMTQDVRDSI 242771
Database: S.parasitica/genome.fa
Posted date: Feb 9, 2011 9:02 AM
Number of letters in database: 53,132,636
Number of sequences in database: 1445
Lambda K H
0.321 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,696,756
Number of Sequences: 1445
Number of extensions: 471356
Number of successful extensions: 3240
Number of sequences better than 1.0e-05: 1
Number of HSP's better than 0.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 94
Number of HSP's that attempted gapping in prelim test: 2110
Number of HSP's gapped (non-prelim): 1998
length of query: 470
length of database: 17,710,878
effective HSP length: 107
effective length of query: 363
effective length of database: 17,556,263
effective search space: 6372923469
effective search space used: 6372923469
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 116 (49.3 bits)